全文获取类型
收费全文 | 1993篇 |
免费 | 172篇 |
国内免费 | 4篇 |
专业分类
林业 | 60篇 |
农学 | 41篇 |
基础科学 | 18篇 |
239篇 | |
综合类 | 383篇 |
农作物 | 85篇 |
水产渔业 | 166篇 |
畜牧兽医 | 934篇 |
园艺 | 53篇 |
植物保护 | 190篇 |
出版年
2021年 | 19篇 |
2020年 | 37篇 |
2019年 | 30篇 |
2018年 | 39篇 |
2017年 | 44篇 |
2016年 | 38篇 |
2015年 | 30篇 |
2014年 | 39篇 |
2013年 | 101篇 |
2012年 | 82篇 |
2011年 | 110篇 |
2010年 | 59篇 |
2009年 | 51篇 |
2008年 | 94篇 |
2007年 | 93篇 |
2006年 | 73篇 |
2005年 | 85篇 |
2004年 | 65篇 |
2003年 | 86篇 |
2002年 | 67篇 |
2001年 | 48篇 |
2000年 | 49篇 |
1999年 | 51篇 |
1998年 | 24篇 |
1997年 | 18篇 |
1995年 | 14篇 |
1993年 | 14篇 |
1992年 | 29篇 |
1991年 | 28篇 |
1990年 | 14篇 |
1989年 | 32篇 |
1988年 | 29篇 |
1987年 | 14篇 |
1986年 | 20篇 |
1985年 | 27篇 |
1984年 | 23篇 |
1983年 | 18篇 |
1982年 | 13篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1979年 | 17篇 |
1978年 | 15篇 |
1977年 | 16篇 |
1974年 | 19篇 |
1973年 | 24篇 |
1972年 | 27篇 |
1971年 | 20篇 |
1970年 | 16篇 |
1968年 | 15篇 |
1966年 | 14篇 |
排序方式: 共有2169条查询结果,搜索用时 31 毫秒
1.
2.
Pilot study of the effect of acemannan in cats infected with feline immunodeficiency virus. 总被引:7,自引:0,他引:7
K M Yates L J Rosenberg C K Harris D C Bronstad G K King G A Biehle B Walker C R Ford J E Hall I R Tizard 《Veterinary immunology and immunopathology》1992,35(1-2):177-189
Acemannan, a complex carbohydrate shown to stimulate interleukin-1, tumor necrosis factor alpha and prostaglandin E2 production by macrophages, has also demonstrated antiviral activity in vitro against human immunodeficiency virus, Newcastle disease virus and influenza virus. A pilot study was undertaken to determine acemannan's effect in 49 feline immunodeficiency virus (FIV) infected cats with clinical signs of disease (Stage 3, 4 or 5), 23 of which had severe lymphopenia. Cats received acemannan either by intravenous (Group 1) or subcutaneous (Group 2) injection once weekly for 12 weeks, or by daily oral (Group 3) administration for 12 weeks. Upon entry into the study, cats were randomly assigned to one of the three groups. Laboratory analyses were performed at the beginning of the study and at Weeks 6 and 12. Cats were allowed to continue with a predetermined maintenance regimen of acemannan after completing the 12-week study. Thirteen cats died during the course of treatment. Upon necropsy, the most frequent histopathologic findings were neoplastic, kidney and pancreatic disease. Friedman's two-way ANOVA test showed no significant differences in efficacy among groups administered acemannan by the different routes. Therefore, groups were combined and a signed-ranks test was used to determine changes over time. A significant increase was seen in lymphocyte counts (P < 0.001). Neutrophil counts decreased significantly (P = 0.007), as did incidence of sepsis (P = 0.008). When cats entering with lymphopenia were analyzed separately, a much greater increase in lymphocyte counts was noted (235%) compared with non-lymphopenic cats (42%). A survival rate of 75% was found for all three groups. Thirty-six of 49 animals are alive 5-19 months post-entry. These results suggest that acemannan therapy may be of significant benefit in FIV-infected cats exhibiting clinical signs of disease. 相似文献
3.
B Mermer P Hillman R Harris T Krogmann Q Tonelli W Palin P Andersen 《Veterinary immunology and immunopathology》1992,35(1-2):133-141
We have developed an antibody detection enzyme-linked immunosorbent assay (ELISA) for the identification of animals infected by feline immunodeficiency virus (FIV). The ELISA solid-phase antigen consists of recombinant FIV gag proteins expressed in bacteria. The proteins are purified from bacterial lysates as insoluble inclusion bodies. In the case of bacterially expressed p24gag, it is shown that all of the linear, sequential epitopes presented by viral p24 during infection are retained. Purified preparations can be substituted for solid-phase whole virus in the IDEXX PetChektm immunoassay. The antibody ELISA duplicates the sensitivity and specificity of the whole virus based PetChek plate assay. 相似文献
4.
H E Kennedy S J McCullough D Graham J Cassidy F E Malone W A Ellis 《Journal of veterinary diagnostic investigation》2001,13(1):30-35
Two serological tests (indirect immunofluorescence and enzyme-linked immunosorbent assay) were developed for the detection of fetal antibody to Chlamydia psittaci. Fetal blood and thoracic fluid from 126 field cases of suspected ovine chlamydial abortion were examined using both tests. Placenta and fetal tissues (lung, liver, and kidney) from the same animals were also examined by the following conventional diagnostic methods: isolation in McCoy cells, detection of chlamydial lipopolysaccharide (LPS), modified Ziehl-Nielsen staining, and direct fluorescent antibody staining of chlamydia in frozen cryostat sections. Seventy cases were positive by fetal serology, and of these, 68 were also positive by isolation and/or LPS detection. The remaining 56 cases had negative fetal serology, and of these, 39 were positive by isolation and/or LPS detection. Results indicate that fetal serology, although less sensitive than either isolation in McCoy cells or detection of chlamydial LPS antigen, may be of particular use when placenta is not available. 相似文献
5.
6.
Maria J. Perestrello de Vasconcelos Bernard P. Zeigler Lee A. Graham 《Landscape Ecology》1993,8(4):273-286
A multi-scale spatial ecological model of a wet sclerophyllous forest subject to recurrent fires is presented. The model is specified in a Discrete Event Systems framework (DEVS) (Zeigler, 1990) interfaced with a Geographic Information System (GIS), and includes the ability to simulate landscape dynamics at several levels of resolution simultaneously. This is achieved by encoding a modular hierarchical representation of the forest landscape components into a set of nested, interconnected, and spatially referenced dynamic models. The results of the landscape dynamics simulations are displayed as sequences of maps through time, illustrating the potential of this modeling methodology for dealing with complex hierarchical structures that operate at several spatial and temporal resolutions.Research work supported by Junta Nacional de Investigaçäo Científica e Tecnológica, Portugal, and the Advanced Resource Technology Program, University of Arizona, USA. 相似文献
7.
8.
9.
Effect of pelleting and beta-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig 总被引:5,自引:0,他引:5
The influences of pelleting and beta-glucanase supplementation on the digestibility of dietary components in a barley-based diet were investigated in pigs fitted with cannulas in the terminal ileum. The diet contained 49.0% starch, 18.9% crude protein and 14.8% dietary fiber, with arabinoxylans (4.9%), mixed-linked beta-glucans (3.4%) and cellulose (3.3%) as the main fiber components. Pelleting increased mixed-linked beta-glucan solubility from 45 to 62%. Neither treatment influenced the ileal or fecal apparent digestibilities of dry matter (64 and 80%, respectively), energy (62 and 79%), crude protein (53 and 75%), crude fat (26 and 27%) or dietary fiber (58 and 72%). However, pelleting increased (P less than .01) the pre-ileal apparent digestibility of starch from 91.5 to 95.3% and decreased (P less than .03) that of ash from -10 to -23%, while increasing (P less than .005) the fecal apparent digestibility of starch from 98.6 to 99.7%. The solubility of mixed-linked beta-glucans in ileal digesta was lower (P less than .001) in pelleted (26%) than in unpelleted (58%) diets. beta-glucanase supplementation also increased (P less than .05) the ileal apparent digestibility of starch, from 92.6 to 94.3%, and of mixed-linked beta-glucans, from 95.7 to 97.1%. Significant between-pig differences in the apparent digestibility of dietary components were observed. In conclusion, treatments that disrupt the endosperm cell walls in barley can increase the proportion of the diet digested prior to the large intestine. 相似文献
10.
T M Frye S N Williams T W Graham 《Veterinary Clinics of North America: Food Animal Practice》1991,7(1):217-275
Deficiencies of vitamins A, D, K, E and thiamin can cause severe limitations in beef production. In particular, vitamin A and E can be common causes of lost profit, secondary to limitations of reproductive and growth potential. Prolonged dry periods will reduce available A and E in pasture forage, as can ensiling and prolonged storage of harvested feedstuffs. Polioencephalomalacia is a thiamin responsive disorder, associated with high concentrate feeding and lush pastures. Antimetabolites, such as amprolium, will cause thiamine deficiency when fed in excess. Recent information has shown improved performance with supplemental beta carotene and niacin. The positive responses in reproductive performance, noted with cattle fed supplemental beta carotene, was independent of vitamin A. Supplementation of vitamins above National Research Council recommendations can be justified. However, proper evaluation of feed and animal status, and documentation of a response to supplementation is necessary before diagnosing deficiencies of specific nutrients. 相似文献