Auswirkungen von Verpilzung und Mykotoxinbehaftung der Futtermittel auf Leistung und Gesundheit bei Schweinen und Rindern     

Klaus Hörügel  Christine Gebhart  Gudrun Hanschmann  Henriette Mietke 《Gesunde Pflanzen》2003,55(5):151-157

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Enterococcus faecium NCIMB 10415 supplementation affects intestinal immune-associated gene expression in post-weaning piglets     

Bianca Siepert  Nicole Reinhardt  Susanne Kreuzer  Angelika Bondzio  Sven Twardziok  Gudrun Brockmann  Karsten Nöckler  Istvan Szabó  Pawel Janczyk  Robert Pieper  Karsten Tedin 《Veterinary immunology and immunopathology》2014,157(1-2):65-77

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Influence of soil and host plant crop on the genetic diversity of Azospirillum amazonense isolates     

Mrcio dos Santos Azevedo  Ktia Regina dos Santos Teixeira  Gudrun Kirchhof  Anton Hartmann  Jos Ivo Baldani 《Pedobiologia》2005,49(6):565-576

The genetic structure of Azospirillum amazonense populations isolated from the rhizosphere soil and washed and surface-sterilised roots of rice, maize and sorghum plants, cropped simultaneously in two different soils (clay loam and sandy loam) was characterised. Genetic diversity was measured by restriction fragment length polymorphism of the amplified 16S–23S rDNA intergenic spacer region (RISA-RFLP) and cluster analysis. Four genetically distinct clusters of isolates were observed with 78% similarity, suggesting that the A. amazonense population was heterogeneous at the strain level regardless of the soil type or host plant. Analysis of molecular variance (AMOVA) demonstrated that the host plant had a highly significant selective effect on the genetic structure of this species, especially on those isolates intimately associated with them, but also to a lesser extent on isolates from the rhizosphere and washed roots. The soil type also had a highly significant selective effect on A. amazonense genetic diversity, especially for those isolates from the rhizosphere soil. The selective effect of the soil type combined with that of the host plant suggests that environmental factors, such as soil texture and composition of exudates provided by C₃ or C₄ plants, play major roles in the overall genetic structure of A. amazonense populations associated with these cereals.  相似文献   

Amalthea's density is less than that of water     

Anderson JD  Johnson TV  Schubert G  Asmar S  Jacobson RA  Johnston D  Lau EL  Lewis G  Moore WB  Taylor A  Thomas PC  Weinwurm G 《Science (New York, N.Y.)》2005,308(5726):1291-1293

Radio Doppler data from the Galileo spacecraft's encounter with Amalthea, one of Jupiter's small inner moons, on 5 November 2002 yield a mass of (2.08 +/- 0.15) x 10(18) kilograms. Images of Amalthea from two Voyager spacecraft in 1979 and Galileo imaging between November 1996 and June 1997 yield a volume of (2.43 +/- 0.22) x 10(6) cubic kilometers. The satellite thus has a density of 857 +/- 99 kilograms per cubic meter. We suggest that Amalthea is porous and composed of water ice, as well as rocky material, and thus formed in a cold region of the solar system, possibly not at its present location near Jupiter.  相似文献   

Postoperative complications in a lamb after major surgery     

Vettorato E  Schoeffmann G  Beard P  Clutton RE 《Veterinary anaesthesia and analgesia》2011,38(1):63-69

INTODUCTION: Anaesthesia in lambs undergoing experimental surgery may develop problems associated with age-related immune incompetency: a postoperative complication in a 3 week old Scottish blackface lamb after spinal surgery is presented. CASE HISTORY AND MANAGEMENT: Both lamb and ewe were in good condition. The ewe was vaccinated against Clostridium perfringens and Clostridium tetani 5 weeks pre-partum. There were no apparent problems with the lamb's intake of colostrum. Pre-anaesthetic medication was intramuscular medetomidine (10 μg kg(-1)). Anaesthesia was induced and maintained with sevoflurane in oxygen. Morphine (0.5 mg kg(-1)), meloxicam (0.6 mg kg(-1)) and ketamine (1 mg kg(-1) followed by 10 μg kg(-1) minute(-1)) were administered intravenously (IV) for perioperative analgesia. Atracurium (0.5 mg kg(-1) IV, followed by 0.17 mg kg(-1) injected when the first twitch of the four, train-of four count was palpated) was used to improve muscle relaxation. The lamb's trachea was intubated and the lungs mechanically ventilated to maintain normocapnia. Intrathecal morphine (0.2 mg kg(-1)), IV meloxicam (0.3 mg kg(-1)) and edrophonium (0.5 mg kg(-1)) were administered before recovery. Operative and initial recovery periods were unremarkable. Three hours after surgery the lamb became depressed. Tachycardia (180-250 beats minute(-1)), tachypnoea (30 breaths minute(-1)), poor peripheral perfusion and cold pelvic limb extremities were present mimicking severe pain, and/or hypovolaemic shock. Analgesics - morphine (total dose 1.3 mg kg(-1)) - and IV fluid therapy boluses - crystalloids (300 mL), colloids (120 mL) and fresh whole blood (60 mL) - failed to ameliorate clinical signs and so the lamb was euthanized 10 hours after surgery. Post-mortem findings supported a possible diagnosis of peracute Clostridium perfringens enterotoxaemia. CONCLUSION: Clostridium perfringens enterotoxaemia should be considered when clinical signs of severe pain and/or hypovolaemic shock fail to respond to analgesics and fluid resuscitation in lambs after major surgery.  相似文献   

Nutrient and persistent pollutant deposition on the Bothnian Bay ice and snow fields     

Lars Rahm  Bertil Håkansson  Per Larsson  Elisabet Fogelqvist  Gudrun Bremle  Jorge Valderrama 《Water, air, and soil pollution》1995,84(1-2):187-201

A study of atmospheric deposited nutrients and some persistent pollutants has been carried out on the ice and snow fields of the subarctic Bothnian Bay, the northernmost basin of the Baltic Sea. Total amounts of 600, 500 and 400 tons NO₃-N, NH₄-N and N_org-N, respectively are deposited in the snow while the corresponding amounts for P_tot-P is 40 tons. The corresponding amount for PCB and lindane are 1.0 and 0.2 kg, respectively. The measurements were carried out on the snow-covered ice four to six weeks old. A part of the deposited snow has been incorporated -into the snow-ice and an attempt to estimate its mean thickness and its amount of nutrients has been made. The total amounts now reach 1700, 1300 and 1100 tons of NO₃-N and NH₄-N and N_org-N, respectively in the snow and ice together. The results obtained support the use of land-based stations in estimates of seasonal atmospheric nutrient deposition to the Bothnian Bay. The observed concentrations of chloroorganic compounds correspond to those land-based observations reported from the same latitude in the northern hemisphere and reported in literature.  相似文献   

Short-term interval baiting to combat the re-emergence of fox rabies in Rhineland Palatinate (Germany) in 2005     

Selhorst T  Freuling CM  Blicke J  Vos A  Larres G  Zimmer K  Fröhlich T  Bätza HJ  Müller TF 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(5-6):191-196

In 2005, the final phase of terrestrial rabies eradication in Germany was put at risk by a severe setback due to re-introduction of the disease in Rhineland-Palatinate from neighbouring Hesse after seven years of absence. The rapid westward spread of the disease prompted veterinary authorities to react swiftly and apply a new yet unproven vaccination strategy to rapidly increase herd immunity in an almost unprotected fox population to stop the epidemic. The cornerstones of this emergency oral rabies vaccination strategy, i. e. vaccination intervals, identification of high risk spots, real time epidemiological assessment, capable to eliminate rabies within 13 months after incursion are described here. This strategy may be used as a template to tackle similar emergency situations in Europe in the future.  相似文献   

Upfront denileukin diftitox as in vivo regulatory T-cell depletion in order to enhance vaccination effects in a canine allogeneic hematopoietic stem cell transplantation model     

Knueppel A  Lange S  Altmann S  Sekora A  Knuebel G  Vogel H  Lindner I  Freund M  Junghanss C 《Veterinary immunology and immunopathology》2012,145(1-2):233-240

Denileukin Diftitox (ONTAK^®, DAB₃₈₉ IL-2) is a recombinant DNA-derived fusion protein depleting cells that express high-affinity IL-2 receptor. Important cell targets are CD4⁺CD25⁺Foxp3⁺ regulatory T cells (T_reg). Elimination of immunosuppressive T_reg by Denileukin Diftitox may provide a way to modulate immune tolerance following stem cell transplantation. Here, we combined T_reg depletion with a vaccination approach to induce donor-specific immune reactions. To investigate this approach we chose the mixed chimerism canine stem cell transplantation model which represents a high state of tolerance between two hematopoietic systems. The aim was therefore to induce a graft versus hematopoiesis effect thereby converting mixed to full donor chimerism. Dog leukocyte antigen identical siblings that had developed a stable mixed chimerism after non-myeloablative stem cell transplantation received a single dose of Denileukin Diftitox (18 μg/kg, i.v.) followed by several cell-lysate vaccinations. Host peripheral blood mononuclear cell lysates combined with CpG-ODN, and Montanide^® ISA 51 were locally applied. In vitro studies demonstrated that canine T_reg are a target of Denileukin Diftitox. The suppression of T-cell proliferation by T_reg was abolished by addition of Denileukin Diftitox (10 nM). An increase of proliferation of median 300% (range: 200%–425%) was observed. No change in donor chimerism was observed after administration of Denileukin Diftitox and vaccination. This study highlights that application of Denileukin Diftitox resulted in a depletion of T_reg followed by an increase of immune response in vitro. This effect could not be confirmed in vivo even if the immune system was stimulated by vaccinations.  相似文献   

Susceptibility of carnivore hosts to strains of canine distemper virus from distinct genetic lineages     

Nikolin VM  Wibbelt G  Michler FU  Wolf P  East ML 《Veterinary microbiology》2012,156(1-2):45-53

Using the complete haemagglutinin (HA) gene and partial phosphoprotein (P) gene we investigated the genotype of canine distemper virus (CDV) strains recovered from two wildlife species in Mecklenburg-Vorpommern, Germany. Phylogenetic analyses demonstrated significant differences between the strains from raccoons Procyon lotor (family Procyonidae) obtained in 2007 and strains from red foxes Vulpes vulpes (family Canidae) obtained in 2008. The raccoon strains belonged to the CDV European wildlife lineage whereas the red fox strains belonged to the CDV Europe lineage. We combined our genetic sequence data with published data from 138 CDV stains worldwide to investigate the proposed importance of amino acid substitutions in the SLAM binding region of the CDV HA protein at position 530 (G/E to R/D/N) and 549 (Y to H) to the spread of domestic dog-adapted CDV strains to other carnivores. We found no evidence that amino acid 530 was strongly affected by host species. Rather, site 530 was conserved within CDV lineages, regardless of host species. Contrary to expectation, strains from non-dog hosts did not exhibit a bias towards the predicted substitution Y549H. Wild canid hosts were more frequently infected by strains with 549Y, a pattern similar to domestic dogs. Non-canid strains showed no significant bias towards either H or Y at site 549, although there was a trend towards 549H. Significant differences between the prevalence of 549Y and 549H in wild canid strains and non-canid strains suggests a degree of virus adaptation to these categories of host.  相似文献   

Structure determination of 3-O-caffeoyl-epi-gamma-quinide, an orphan bitter lactone in roasted coffee     

Frank O  Blumberg S  Krümpel G  Hofmann T 《Journal of agricultural and food chemistry》2008,56(20):9581-9585

Recent investigations on the bitterness of coffee as well as 5- O-caffeoyl quinic acid roasting mixtures indicated the existence of another, yet unknown, bitter lactone besides the previously identified bitter compounds 5- O-caffeoyl- muco-gamma-quinide, 3- O-caffeoyl-gamma-quinide, 4- O-caffeoyl- muco-gamma-quinide, 5- O-caffeoyl- epi-delta-quinide, and 4- O-caffeoyl-gamma-quinide. In the present study, this orphan bitter lactone was isolated from the reaction products generated by dry heating of 5- O-caffeoylquinic acid model, and its structure was determined as the previously unreported 3- O-caffeoyl- epi-gamma-quinide by means of liquid chromatography-mass spectrometry (LC-MS) and one-/two-dimensional NMR experiments. The occurrence of this bitter lactone, exhibiting a low bitter recognition threshold of 58 micromol/L, in coffee beverages could be confirmed by LC-MS/MS (negative electrospray ionization) operating in the multiple reaction monitoring mode.  相似文献