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1.
Victor JV Rossetto Lígia SLS da Mota Noeme S Rocha Hélio A Miot Fabrizio Grandi Cláudia VS Brand?o 《Acta veterinaria Scandinavica》2013,55(1):39
Background
Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.Results
We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson''s Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.Conclusions
We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS. 相似文献2.
Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis,angiogenesis and survivability of cultured buffalo luteal cells 
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下载免费PDF全文 SR Mishra MS Parmar VP Yadav R Reshma J Bharati MK Bharti A Paul VS Chouhan G Taru Sharma G Singh M Sarkar 《Reproduction in domestic animals》2016,51(6):855-869
The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P4) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P4 secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P4 secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P4 secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL. 相似文献
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Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus 下载免费PDF全文
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Katherine A Damcevski Greg Dojchinov James D Woodman Victoria S Haritos 《Pest management science》2010,66(4):432-438
BACKGROUND: The ethyl formate/carbon dioxide (CO2) formulation Vapormate? is a rapid‐acting fumigant being developed for the control of stored‐grain insects. The effects have been investigated of concentration, exposure times of 1, 3, 24 and 72 h and two grain temperatures, 15 and 25 °C, on its efficacy against mixed‐stage cultures of Sitophilus oryzae (L.) Tribolium castaneum (Herbst) and strongly phosphine‐resistant Rhyzopertha dominica (F.) strain QRD569. RESULTS: High mortalities (≥92%) of mixed‐stage cultures of all three species were obtained when grain was fumigated with the formulation (193 g m?3 ethyl formate) for 1 h. Complete control of R. dominica QRD569 and T. castaneum was achieved with 63 and 76 g m?3 ethyl formate respectively, with exposure for 24 h, whereas mean mortality of S. oryzae was 86% under the same conditions. Mortalities of S. oryzae juvenile stages were significantly lower than adults under the conditions tested, which was due to pronounced tolerance of mid‐stage pupae to the fumigant. Reducing grain temperature from 25 to 15 °C had no effect on insect mortality. CONCLUSION: Ethyl formate/CO2 formulation is highly effective against stored‐grain insects over a range of concentrations and exposure times. Efficacious fumigations were conducted in as little as 1 h, and a strongly phosphine‐resistant R. dominica strain was readily controlled with the fumigant. Copyright © 2009 CSIRO, Australia. Published by John Wiley & Sons, Ltd 相似文献
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Ronan A. Mullins MVB DECVS Carlos Sanchez Villamil DVM Laura E. Selmic BVetMed MPH DACVS-SA DECVS Michael S. Tivers BVSc PhD DECVS J. Brad Case DVM MS DACVS Ameet Singh BSc DVM DVSc DACVS Kelley M. Thieman Mankin DVM MS DACVS Davina M. Anderson MA VetMB PhD DSAS DECVS Robert N. White BSc BVetMed DSAS DECVS SFHEA Kathryn M. Pratschke MVB MVM DECVS Hilde de Rooster DVM MVM PhD DECVS Anne Kummeling DVM PhD DECVS Donald A. Yool BVMS PhD DECVS SFHEA Melanie Olive DVM Jean-Philippe Billet Dr vét DECVS Ines Gordo DVM MS Herve Brissot DV DECVS Cameron Broome BVSc DVCS FANZCVS Barbara M. Kirby DVM MS DACVS DECVS 《Veterinary surgery : VS》2020,49(5):958-970
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Gregory B. Daniel DVM MS Leslie Wantschek DVM Ronald Bright DVM MS Ilse Silva-Krott VS MS 《Veterinary radiology & ultrasound》1990,31(4):182-185
Radionuclide angiography was used to document occlusion of the distal aorta by thromboemboli in two dogs. Findings were confirmed by necropsy. The location of the thrombus corresponded with the scintigraphic lesion. Information obtained by the radionuclide angiogram was instrumental in patient management. We suggest the use of radionuclide angiography as a quick, non-invasive alternative to contrast angiography in animals suspected of having aortic thromboembolism. 相似文献
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Assessing the spatial distribution of Tuta absoluta (Lepidoptera: Gelechiidae) eggs in open‐field tomato cultivation through geostatistical analysis 下载免费PDF全文
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VS Chouhan RP Panda VP Yadav V Babitha FA Khan GK Das M Gupta SS Dangi G Singh S Bag GT Sharma B Berisha D Schams M Sarkar 《Reproduction in domestic animals》2013,48(5):810-818
The aim of this study was to document the expression and localization of VEGF system comprising of VEGF isoforms (VEGF 120, VEGF 164 and VEGF 188) and their receptors (VEGFR1 and VEGFR2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors. In general, all the components of VEGF system (the VEGF isoforms and their receptors) were found in the water buffalo CL during the oestrous cycle. The mRNA as well as protein expression of VEGF system was highest during the early and mid‐luteal phase, which later steadily decreased (p < 0.05) after day 10 to reach the lowest level in regressed CL. As demonstrated by immunohistochemistry, VEGF protein was localized predominantly in luteal cells; however, VEGFR1 and VEGFR2 were localized in luteal cells as well as in endothelial cells. In conclusion, the dynamics of expression and localization of VEGF system in buffalo corpora lutea during the luteal phase were demonstrated in this study, indicating the possible role of VEGF system in the regulation of luteal angiogenesis and proliferation of luteal as well as endothelial cells through their non‐angiogenic function. 相似文献