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Ashalatha?S.?Nair Chee?How?Teo Trude?Schwarzacher Pat?Heslop?HarrisonEmail author 《Euphytica》2005,143(3):285-290
Summary The banana cultivars are originated from the intra- and inter-specific hybridization of two wild diploid species, Musa acuminata Colla and Musa balbisiana Colla, contributing the A and B genomes, respectively. They are classified into genomic groups by scoring morphological features.
Molecular markers provide a quick and reliable system of genome characterization and manipulation in breeding lines. In the
present study a PCR based molecular marker specific for B genomes is been reported. The IRAP primer, designed based on the
LTR sequence of banana Ty3-gypsy-like retroelement (Musa acuminata Monkey retrotransposon, AF 143332), was used to identify the B genome in the banana cultivars. Further a primer pair designed
from B specific bands of Musa balbisiana `Pisang Gala' was used to classify AAB and ABB cultivars in the collection. Among the 36 cultivars tested with this primer,
the B specific band was absent in the AA and AAA cultivars (except in one AAA and AAB cultivar) but present in all other AB,
AAB and ABB cultivars. Among the triploid AAB/ABB, the PCR products with B specific primers showed restriction pattern polymorphism
with AluI. In ABB genomes the band intensity was high whereas low intensity band observed in AAB genomes. Four cultivars reported
to have the ABB genome showed a pattern similar to AAB, and one cultivar reported to have AAA genome showed a pattern similar
to ABB genome, suggesting missampling or misidentification. The primers used in this study are useful to identify the presence
of B genome in banana cultivars, and band intensity may be a preliminary indicator of ploidy level of the B genome but needs
further studies with competitive PCR for clarification.
These authors contributed equally in this paper. 相似文献
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P How 《The Journal of small animal practice》1966,7(1):25-28
Abstract— A general discussion of the application of heating and ventilating engineering to practical construction in new and converted premises. Résumé— Une discussion générale pour l'application des plans de chauffage et d'aération à la construction pratique dans des locaux nouveaux ou transformés. Zusammenfassung— Eine allgemeine Besprechung über den Einbau von Heizungs- und Lüftungsanlagen bei sachdienlichen Konstruktionen in neuen und umgebauten Gebäuden. 相似文献
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Hypocuprosis without clinical symptoms in single-sucked calves 总被引：1，自引：0，他引：1
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Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported large numbers of differences between DNA and messenger RNA in human cells, indicating unprecedented levels of RNA editing, and including sequence changes not produced by any of the known RNA editing mechanisms. However, common sources of systematic errors in high-throughput sequencing technology, which were not properly accounted for in this study, explain most of the claimed differences. 相似文献
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The aim of the present study was to establish the technology of intracytoplasmic sperm injection (ICSI) in rabbit by using the sperm frozen without cryoprotectants. Observation under an electron microscope revealed that the rabbit spermatozoa frozen without cryoprotectants had severe damage especially in the plasma membrane and junction between head and tail. However, after being injected into the oocytes, the sperm frozen without cryoprotectants retained the capability of supporting the cleavage and development of the ICSI oocytes, with no significant difference from that of fresh sperm, although the development of ICSI embryos derived from either frozen sperm or fresh sperm is much lower than that of in vivo‐fertilized zygotes. When additional artificial activation was applied following ICSI, the rates of cleavage and blastocyst formation of ICSI oocytes were significantly increased when compared with the oocytes without additional activation. Yet, the cell numbers in blastocysts were not significantly different between the activation and non‐activation group. After embryo transfer, four offspring were obtained from the oocytes microinjected with the sperm frozen without cryoprotectants. The technology established by this study may facilitate exploring the ICSI‐based transgenic method in rabbit and broaden the application of ICSI technique in related field. 相似文献
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长期以来，在生物制药行业，油佐剂疫苗的定量灌装成了令各个生产厂家头疼的问题。因疫苗中含有一定的油佐剂成分，所以黏度较大，在灌装过程中速度提高不上去，灌装量不稳定。随着GMP的进一步落实，药机行业更新换代，这些问题也逐步得到改善。罐装机械不仅从材质、结构、原理上发生改变，而且控制模式也发生较大变化。尽管如此，一些细小环节，仍对罐装速度及稳定性有一定影响。 相似文献
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Chee?How?Teo Han?Pin?Pui Rofina?Yasmin?Othman Jennifer?Ann?HarikrishnaEmail author 《Genetic Resources and Crop Evolution》2011,58(5):713-725
The Argonaute proteins are key components in the effector complex of RNA silencing pathways which interact with small RNAs
to mediate sequence specific silencing of nucleic acid targets. In plants, these proteins are involved in a diversity of biological
roles such as antiviral defence, heterochromatin regulation and in the regulation of growth and development. This report describes
the study of Argonaute gene sequences in bananas which are an important food staple for many developing nations. This study successfully isolated
AGO7-specific PIWI domain genomic sequences from 12 diploid Musa species of three Musa sections and also from Ensete. The Musa AGO7-specific PIWI domain sequences showed the highest similarity to rice AGO7/SHOOTLESS4 with an average amino acid identity
of 78%. Phylogenetic analysis of the Musa sequences revealed phylogenetic grouping that agrees fairly well with the present knowledge of the taxonomic classification
of Musa species. In addition, this study estimated that there are at least 15 Argonaute genes or loci containing PIWI domain sequences in the genome of Musa acuminata ssp. malaccensis. 相似文献
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甲酰胺为伊维菌素生产过程中的残留物质，为确保本品的安全，须建立其含量测定方法。本试验采用气相谱法、火焰离子（FID）检测器两种方法进行测定。甲酰胺在2－10μl/ml范围内，峰面积与其浓度呈良好线性关系。建立的定量方法简便，准确，可用于甲酰胺的含量测定。 相似文献