Detection of chlamydial antibody by fetal serology--an aid to the diagnosis of ovine abortion.     

H E Kennedy  S J McCullough  D Graham  J Cassidy  F E Malone  W A Ellis 《Journal of veterinary diagnostic investigation》2001,13(1):30-35

Two serological tests (indirect immunofluorescence and enzyme-linked immunosorbent assay) were developed for the detection of fetal antibody to Chlamydia psittaci. Fetal blood and thoracic fluid from 126 field cases of suspected ovine chlamydial abortion were examined using both tests. Placenta and fetal tissues (lung, liver, and kidney) from the same animals were also examined by the following conventional diagnostic methods: isolation in McCoy cells, detection of chlamydial lipopolysaccharide (LPS), modified Ziehl-Nielsen staining, and direct fluorescent antibody staining of chlamydia in frozen cryostat sections. Seventy cases were positive by fetal serology, and of these, 68 were also positive by isolation and/or LPS detection. The remaining 56 cases had negative fetal serology, and of these, 39 were positive by isolation and/or LPS detection. Results indicate that fetal serology, although less sensitive than either isolation in McCoy cells or detection of chlamydial LPS antigen, may be of particular use when placenta is not available.  相似文献   

Preventing nuclear war     

Malone TF 《Science (New York, N.Y.)》1984,223(4634):340

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Effect of heat stress on the behavioral and physiological patterns of Small-tail Han sheep housed indoors     

Li  F. K.  Yang  Y.  Jenna  K.  Xia  C. H.  Lv  S. J.  Wei  W. H. 《Tropical animal health and production》2018,50(8):1893-1901

Tropical Animal Health and Production - This study was conducted to investigate the effects of heat stress on the behavioral and physiological patterns in Small-tail Han sheep housed indoors in...  相似文献   

Empirical analysis and prediction of nitrate loading and crop yield for corn-soybean rotations     

R.W. Malone  L. Ma  T. Meade  P. Heilman  J.L. Hatfield 《Geoderma》2007,140(3):223-234

Nitrate nitrogen losses through subsurface drainage and crop yield are determined by multiple climatic and management variables. The combined and interactive effects of these variables, however, are poorly understood. Our objective is to predict crop yield, nitrate concentration, drainage volume, and nitrate loss in subsurface drainage from a corn (Zea mays L.) and soybean (Glycine max (L.) Merr.) rotation as a function of rainfall amount, soybean yield for the year before the corn-soybean sequence being evaluated, N source, N rate, and timing of N application in northeastern Iowa, U.S.A. Ten years of data (1994-2003) from a long-term study near Nashua, Iowa were used to develop multivariate polynomial regression equations describing these variables. The regression equations described over 87, 85, 94, 76, and 95% of variation in soybean yield, corn yield, subsurface drainage, nitrate concentration, and nitrate loss in subsurface drainage, respectively. A two-year rotation under average soil, average climatic conditions, and 125 kg N/ha application was predicted to loose 29, 37, 36, and 30 kg N/ha in subsurface drainage for early-spring swine manure, fall-applied swine manure, early-spring UAN fertilizer, and late-spring split UAN fertilizer (urea ammonium nitrate), respectively. Predicted corn yields were 10.0 and 9.7 Mg/ha for the swine manure and UAN sources applied at 125 kg N/ha. Timing of application (i.e., fall or spring) did not significantly affect corn yield. These results confirm other research suggesting that manure application can result in less nitrate leaching than UAN (e.g., 29 vs. 36 kg N/ha), and that spring application reduces nitrate leaching compared to fall application (e.g., 29 vs. 37 kg N/ha). The regression equations improve our understanding of nitrate leaching; offer a simple method to quantify potential N losses from Midwestern corn-soybean rotations under the climate, soil, and management conditions of the Nashua field experiment; and are a step toward development of easy to use N management tools.  相似文献   

Simulating management effects on crop production, tile drainage, and water quality using RZWQM-DSSAT   总被引：1，自引：0，他引：1  

S.A. Saseendran  R. Malone  L.R. Ahuja  D.L. Karlen 《Geoderma》2007,140(3):297-309

The objective of this study was to explore if more crop-specific plant growth modules can improve simulations of crop yields, and N in tile flow under different management practices compared with a generic plant growth module. We calibrated and evaluated the Root Zone Water Quality Model (RZWQM) with the Decision Support for Agrotechnology Transfer (DSSAT v3.5) plant growth modules (RZWQM-DSSAT) for simulating tillage (NT — no till, RT — ridge till, CP — chisel plow, and MP — moldboard plow), crop rotation {CC — continuous corn, and CS — corn (Zea mays L.)-soybean [Glycine max (L.) Merr.]}, and nitrogen (N) (SA — single application at preplant, and LSNT — late spring soil N test based application) and manure (SM — fall injected swine manure) management effects on crop production and water quality. Data from 1978 to 2003 from a water quality experiment near Nashua (Nashua experiments), Iowa, USA, were used. The model was calibrated using data from one treatment plot and validated for the rest of the plots. Simulated management effects on annual N loading in tile flow were agreeable with measured effects in 85%, 99%, 88%, and 78% of the cases for tillage, crop rotation (CS vs. CC), N application timing (SA vs. LSNT), and swine manure applications (SM vs. SA), respectively. On average, the LSNT plots were simulated to have 359 kg ha^− 1 higher corn yield compared to SA, when the observed increase was 812 kg ha^− 1. Grain yield simulations were not sensitive to differences between RT and NT, between SM and SA treatments, and between CS and CC. We conclude that considering the uncertainties of basic input data, processes in the field, and lack of site specific weather data, the results obtained with this RZWQM-DSSAT hybrid model were not much better than the results obtained earlier with the generic crop growth module.  相似文献   

Investigations into an outbreak of tuberculosis in a flock of sheep in contact with tuberculous cattle     

Malone FE  Wilson EC  Pollock JM  Skuce RA 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(10):500-504

Tuberculosis, associated with Mycobacterium bovis infection, occurs infrequently in sheep. A sheep flock, which was potentially exposed to a high level of infection from in-contact tuberculous cattle, was examined for evidence of infection. Six sheep that had given a positive reaction to the comparative intradermal tuberculin test were examined post mortem. Tuberculous lesions were present in four of these sheep. Lesion morphology and distribution in the sheep was similar to that in cattle. M. bovis was cultured from the lesions and the isolates were strain typed by spoligotyping and variable number of tandem repeats (VNTR) typing. Tuberculin-reacting sheep also reacted positively to an assay for in vitro release of interferon-gamma. This paper describes the first report of an outbreak of tuberculosis in sheep in either Britain or Ireland. The report describes immunology and pathology findings and, using molecular typing techniques, suggests that the sheep had been infected from in-contact cattle.  相似文献   

A cross-sectional coprological survey of liver flukes in cattle and sheep from an area of the southern Italian Apennines   总被引：3，自引：0，他引：3  

Cringoli G  Rinaldi L  Veneziano V  Capelli G  Malone JB 《Veterinary parasitology》2002,108(2):137-143

A cross-sectional coprological survey of liver flukes (Fasciola hepatica and Dicrocoelium dendriticum) was conducted on 81 bovine farms and 197 ovine farms with animals pasturing in an area (3971 km(2)) of the southern Italian Apennines. The farms were selected to be uniformly distributed throughout the study area using geographical information system (GIS) software. Between June 1999 and March 2000, faecal samples were collected from 975 cattle and 3940 sheep and examined using a modified McMaster technique. The results were subjected to statistical analysis and point distribution maps (PDMs) were drawn by GIS.Cattle of 9 of the 81 (11.1%) farms were positive for F. hepatica and of 43 (53.1%) for D. dendriticum. Sheep of 8 of the 197 (4.1%) farms were positive for F. hepatica and of 133 (67.5%) for D. dendriticum. Co-infection was found in cattle of 2 (2.5%) farms, and in sheep of 8 (4.1%) farms.The findings of the present survey show that D. dendriticum was the predominant liver fluke found in cattle and sheep with respect to egg count numbers for both farms and animals. In addition, the general trends of the PDMs show that D. dendriticum was widely and homogeneously spread throughout the study area, whereas F. hepatica was present only in a few concentrated zones of the study area that had both positive bovine and positive ovine farms.  相似文献   

Molting and Mortality of Red Swamp and White River Crawfish Subjected to Eyestalk Ablation: A Preliminary Study for Commercial Soft-Shell Crawfish Production   总被引：2，自引：0，他引：2  

Shulin  Chen Ronald F.  Malone  Jay V.  Huner 《Journal of the World Aquaculture Society》1993,24(1):48-57

Eyestalk ablation may reduce the cost of soft-shell crawfish production by reducing the molt interval. In this study, both immature and mature red swamp crawfish Procambarus clarkii and white river crawfish Procambarus zonangulus , formally Procambarus actus actus (Hobbs and Hobbs 1990), were ablated using a pair of modified pliers and placed in a recirculating system. Molting percentages, mortalities and mean molt intervals of the ablated crawfish were analyzed.
Eyestalk ablation resulted in dramatic reduction of molt intervals and mortalities comparable with the current commercial (non-ablation) soft-shell crawfish production systems. The mean molt intervals of the ablated red swamp crawfish ranged from 6.7 to 7.8 days for immature and 9.2 days for mature animals; whereas, the molt interval of ablated white river crawfish was 8.9 and 11.2 days for immature and mature animals, respectively. Mortalities obtained in this study ranged from 20 to 48% and 32 to 66% for immature and mature crawfish, respectively. During the experiments, molting percentages and mortalities were not consistent. Secondary treatments such as air clotting and cauterization did not alter mortality significantly.  相似文献   

Bench-Scale Evaluation of a Micro-Computer Automated Algal Turbidostat   总被引：1，自引：0，他引：1  

Kelly A.  Rusch Ronald F.  Malone 《Journal of the World Aquaculture Society》1993,24(3):379-389

A bench-scale, micro-computer automated algal turbidostat was constructed and its production and culture maintenance capabilities were evaluated. The computer program "Supervisor," written in Turbo Pascal, consisted of a series of functions and procedures integrated through a core program consisting of a: 1) stack; 2) bubble sort routine; and 3) supervisor procedure. The program controlled the daily routine procedures including harvesting, feeding, monitoring and a semimonthly disinfection process. Data received from the monitoring devices were used by the computer for decision making processes concerning changes in operation. In addition to reducing labor, continuous computer monitoring insured more reliable system operation.
The system operated successfully for five months, reliably producing Chlorella minutissima . For each harvesting event, the percent volume removed from each chamber depended on the solar cell reading recorded immediately before the harvest cycle began. Significant correlations between millivolts and algal biomass concentrations allowed the use of solar cells as a reliable and inexpensive method of culture density management. Kinetic studies using Chlorella minutissima resulted in a maximum biomass concentration of approximately 190 g/m³ and an average specific growth rate of 0.66/d. Experimental data combined with a first order computer analysis projected a daily harvest of 82 g/m³/d (dry weight) based on a 38% turnover rate, the average observed harvest rate for the three chambers.  相似文献   

A mathematical model for soft-shell crawfish production     

Shulin Chen  R.F. Malone 《Aquacultural Engineering》1992,11(4):217-229

A mathematical model was developed for soft-shell crawfish production management based on the assumption that the crawfish molt interval follows a normal distribution. The mean and standard deviation of the molt interval, the two main parameters of the model, were estimated to be 48 and 15 days, respectively, based on experimental data for the red swamp crawfish (Procambarus clarkii). The model was further verified through comparison with experimental data. Simulations using the model were conducted to evaluate the impact of mortality and management strategies upon soft-shell crawfish production. The results indicated that mortality is a major factor in determining the production rate of a soft-shell crawfish facility. For a 6-month operation period, the predicted total production is 263% of the loading capacity for a mortality rate of 1%/day. This production will drop to 101% of the loading capacity with a mortality rate of 5%/day. Besides variations in the mortality rate, loading and replacement strategies also affect soft-shell crawfish operation, as demonstrated by the simulation results.  相似文献