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The efficacy of the commercial glyphosate [( N -phosphonomethyl) glycine] formulations Roundup Ultra, Touchdown and Engame were compared for the control of prickly sida ( Sida spinosa L.), morningglory ( Ipomeae hederacea var. integriuscula Gray), sicklepod ( Senna obtusifolia L.) and purple nutsedge ( Cyperus rotundus L.). Engame is a new formulation of glyphosate that contains glyphosate acid and 1-aminomethanamide dihydrogen tetraoxosulfate (AMADS), a proprietary mixture of sulfuric acid and urea, other than glyphosate salt and surfactants. Injury by Engame differed from Roundup Ultra and Touchdown in that necrotic lesions formed on leaves several hours after treatment. Leaves of very susceptible species, such as prickly sida, were rapidly, although incompletely, desiccated and then became chlorotic and died in a manner typical of other glyphosate formulations. Engame was 2–3 times more active to growth inhibition than either the Roundup Ultra or Touchdown formulations, based on GR50 comparisons expressed on an acid equivalent basis. The GR50 estimates did not change over the 3 week evaluation period for prickly sida and purple nutsedge, and after 2 weeks after treatment for morningglory. The GR50 estimates for sicklepod decreased over the 3 week evaluation period indicating a slower response to glyphosate. The application of AMADS alone caused minute necrotic lesions on sicklepod and purple nutsedge, and lesions up to 3 mm in diameter on prickly sida and morningglory. Further injury from AMADS was not noted and plants resumed growth without apparent delay. At glyphosate rates above 1120 g ha−1 , greater than 80% control was achieved at 7 days after treatment. These results demonstrate that glyphosate efficacy can be further enhanced by formulations that apparently improve uptake and translocation. 相似文献
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MTB-951 is a plant pathogen ( Drechslera monoceras ), which was isolated from native Echinochloa spp. in Japan. The conidia of this pathogen were used as the herbicidal active ingredient to control Echinochloa crus-galli L. The herbicidal efficacy of MTB-951 on E. crus-galli was drastically increased with an increasing water depth of between 1 and 10 cm. The efficacy also was increased when shoots were lodged by pushing down on them with a stainless steel net so that all parts of the weed were submerged. These results suggest that the contact area of the leaf surface of E. crus-galli with water is important for infection and that lodging the shoots of E. crus-galli might be effective in increasing the herbicidal efficacy. In order to find other methods to lodge the weed, we investigated several materials, of which diisononyl phthalate (DINP) was found to be the most effective. Using this material as a model, the effect of lodging on the herbicidal efficacy of MTB-951 was examined. The lodging ratio (ratio of the number of the plants lodged on the water surface to that of the total plants) was increased with an increased amount of DINP, between 0.5 and 8 kg ha−1 . The herbicidal efficacy of MTB-951 also was increased by DINP and a positive correlation was observed between the lodging ratio and herbicidal efficacy. These results indicate that lodging, which can increase the contact area between the leaf surface and water, enhances the herbicidal efficacy of MTB-951. 相似文献
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The enzyme, aryl acylamidase, was characterized in propanil-susceptible and propanil-resistant barnyardgrass with respect to kinetic parameters, the effects of inhibitors, and the levels of activity in dark- and light-grown tissues. The enzyme reaction in the resistant tissue preparation proceeded linearly with time over a 5 h time course, while activity in the susceptible tissue preparation was 2- to 4-fold lower and the activity tended to decrease after 2 h. The apparent Km values were 62.1 mmol L−1 and 3.1 mmol L−1 for the enzyme activity in the susceptible and resistant tissue preparations, respectively. Two herbicides (anilofos and piperophos), previously shown to synergize propanil injury against the resistant biotype, were found to be potent inhibitors of the in vitro aryl acylamidase activity. 相似文献
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KANGETSU HIRASE SATOSHI YOSHIGAI MAKOTO NISHIDA TATSUO SHINMI 《Weed Biology and Management》2004,4(4):213-217
MTB-951 is a potential mycoherbicide using a fungal plant pathogen ( Drechslera monoceras ) isolated from native Echinochloa species in Japan. Conidia of this pathogen were used as the active ingredient and the effect of temperature on its properties was examined in a laboratory. The optimum temperature for conidium germination was broad between 15 and 35°C and no difference in the germination ratio existed within this temperature range. Mycelial growth was the fastest at 25°C but was slower outside of the range 15–35°C. When leaves of Echinochloa crus-galli L. were immersed in deionized water containing the conidia, cellular electrolyte leakage from the leaves was observed. However, no electrolyte leakage occurred from leaves of rice ( Oryza sativa L.) under the same conditions. The occurrence of electrolyte leakage from E. crus-galli was dependent on the temperature and was the highest at 25°C. Herbicidal activity of MTB-951 against E. crus-galli was high between 25°C and 30°C, but decreased at 35°C in a glass vessel. In the present study, it was revealed that the physiological and herbicidal properties of MTB-951 depended on the temperature. 相似文献
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The conidia of a plant pathogen ( Drechslera monoceras [Drechsler] Subram. et Jain [= Exserohilum monoceras [Drechsler] Leonard et Suggs]), MTB-951, which was isolated from native Echinochloa species in Japan, were used as the herbicidal active ingredient. The effects of δ-aminolevulinic acid (ALA) on the efficacy of foliar-applied MTB-951 on Echinochloa crus-galli L. were examined. First, foliar-applied MTB-951 exhibited almost no efficacy when no dew period was provided. However, the efficacy increased depending on the length of the dew period. MTB-951 and ALA exhibited an additive effect on E. crus-galli under 3 day dew conditions, although they exhibited a significant synergistic effect under no-dew conditions. When the leaves of E. crus-galli were immersed in deionized water containing the conidia of MTB-951, cellular electrolyte leakage from the leaves was observed. The ALA increased the electrolyte leakage by MTB-951 synergistically. Also, ALA increased the number of lesions on the leaves of E. crus-galli caused by MTB-951 under light conditions but failed to increase the number under dark conditions. These results suggest that ALA promotes infection with MTB-951 for E. crus-galli through its photodynamic action. 相似文献
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The effects of surfactants and simulated rain were investigated on the efficacy of Engame and Roundup Ultramax formulations of glyphosate on johnsongrass ( Sorghum halepense L.), prickly sida ( Sida spinosa L.) and yellow nutsedge ( Cyperus esculentus L.). Flame surfactant provided the greatest enhancement of Engame efficacy and the effect was species-dependent. Flame enhanced the activity of Engame on johnsongrass and yellow nutsedge but not on prickly sida. Engame and Engame plus Flame were more active than Roundup Ultramax on a glyphosate acid-equivalent basis on johnsongrass without rain, and on yellow nutsedge with or without rain. The Engame and Roundup Ultramax activities on johnsongrass were similar with rain, and rain occurring between five and 30 min after treatment diminished their activities to < 38% of the control. With the addition of Flame surfactant, Engame activity on johnsongrass increased, such that 50% and 80% of the control were realized, even with rain occurring between five and 15 min after treatment, respectively. Engame and Roundup Ultramax provided better control of prickly sida than of johnsongrass following a rain event. The addition of Flame surfactant to Engame did not enhance the activity on prickly sida. Yellow nutsedge control with Engame and Engame plus Flame was greater than with Roundup Ultramax and rain had little effect on control regardless of the length of the rain-free period. These results demonstrated that the activities of Engame, Engame plus Flame and Roundup Ultramax were species-dependent and surfactant-dependent. 相似文献
7.
Sulfur assimilation in plants and weed control: Potential targets for novel herbicides and action sites of certain safeners 总被引：1，自引：0，他引：1
Sulfur is an indispensable element for plants. It is found in sulfur-containing amino acids, cysteine and methionine, and in various other important biochemical components and processes. Inhibitors of sulfur assimilation, or cysteine and methionine synthesis, could be potential herbicides. In the present paper, the sulfur assimilation pathway in plants is described, followed by the introduction of several compounds (inhibitors and safeners) acting on this pathway. Uptake of inorganic sulfate through the roots is the first step of sulfur assimilation in plants. Sulfate is reduced mainly in chloroplasts to sulfide by a multistep process, and sulfide is then incorporated into cysteine. Cysteine is converted to cystathionine, homocysteine and methionine. Cysteine is incorporated into glutathione (GSH) by γ-glutamylcysteine synthetase and GSH synthetase. Three enzymes involved in cysteine and methionine biosynthesis, cysteine synthase, cystathionine γ-synthase and cystathionine β-lyase, have been investigated as target sites for herbicides. Several inhibitors of these enzymes (e.g. rhizobitoxine and propargylglycine) were also phytotoxic, suggesting that the synthetic pathway of sulfur-containing amino acids could be a new target site for herbicides. Some safeners for herbicides were found to act on the sulfur assimilation pathway and on GSH synthesis to increase GSH, which can be involved in herbicide metabolism and detoxification. Several safeners elevate GSH levels by increasing the activities of enzymes involved in sulfur assimilation and GSH synthesis. Further studies on plant sulfur metabolism may lead to the discovery of new herbicides and to the comprehensive understanding of the mode of action of safeners. 相似文献
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In vitro assay procedures for measuring the activity of cysteine biosynthesis from serine (CBS), which is a coupled reaction catalyzed by serine acetyltransferase and cysteine synthase, were developed using crude extracts from sorghum shoots. Cysteine biosynthesis from serine activity was dependent on acetyl‐CoA concentrations (up to 1.5 mmol L?1), serine (at least up to 20 mmol L?1) and sulfide (up to 0.25 mmol L?1), respectively, and was proportional to the protein concentration in the reaction mixture below 0.4 mg mL?1. The reaction rate was 6.6 nmol min?1 per mg of protein during the first 5 min, but increased to 45.6 nmol min?1 per mg of protein between 30 and 45 min after reaction initiation. Sorghum had the highest CBS total activity (222.4 nmol min?1 per g of fresh weight), and large crabgrass had the lowest CBS total activity (4.7 nmol min?1 per g of fresh weight) when CBS activity in shoots was extracted from sorghum, corn, johnsongrass, barnyardgrass, goosegrass, green foxtail and large crabgrass. Similar results were obtained for CBS specific activity (nmol min?1 per mg of protein). There was no correlation between total CBS activity and susceptibility to metolachlor; however, when corn was excluded, a correlation of R2 = 0.690 was found. Flurazole seed treatment (1.25 g per kg of seed) conferred metolachlor resistance by sorghum, and enhanced total CBS activity and non‐protein thiol content by 27 and 61%, respectively. The increase in thiol content presumably contributed to metolachlor tolerance in sorghum. From these results, the difference in CBS activity partially contributes to the selectivity to metolachlor among certain grass species, and to the safening action of flurazole by increasing thiol content. 相似文献
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KANGETSU HIRASE SATOSHI YOSHIGAI MAKOTO NISHIDA KAZUNORI TAKANAKA TATSUO SHINMI 《Weed Biology and Management》2004,4(2):71-74
MTB-951 is a potential mycoherbicide using a fungal plant pathogen ( Drechslera monoceras ) isolated from native Echinochloa species in Japan. Conidia of this pathogen were used as the active ingredient and its herbicidal performance was examined in a greenhouse. The efficacy of MTB-951 on Echinochloa crus-galli L. was higher in deep water (7–9 cm) than in relatively shallow water (3–5 cm). In a postemergence application, the efficacy decreased as the leaf stage of E. crus-galli proceeded between the 1 and 2.5 leaf stage. For example, the control ratio (%) of E. crus-galli was 95% when applied at the 1 leaf stage, and 72% at the 2.5 leaf stage in 5 cm water. Generally, mycoherbicidal efficacy was less when applied pre-emergence rather than postemergence. Efficacy was also influenced by the duration of submergence in deep water. For example, when water depth was kept at 5 cm for more than 7 days after application and then decreased down to 3 cm, the efficacy was high. However, when the water depth was kept at 5 cm for less than 7 days, the efficacy was low. Efficacy was lower under high temperatures (35°C/25°C, day/night) than under low temperatures (25°C/15°C, day/night). Water management, application timing and temperature are important factors on herbicidal efficacy of MTB-951 to control E. crus-galli . 相似文献
10.
A 4 year field study was conducted from 1998 to 2001 to evaluate the response of prickly sida ( Sida spinosa L.) and hyssop spurge ( Euphorbia hyssopifolia L.) growing in either ultra narrow row (UNR) or wide row (WR) cotton ( Gossypium hirsutum L.) management systems. Weeds surviving pre- and postemergence herbicide treatments were harvested just prior to defoliation and the morphological characteristics were compared. Prickly sida growth was significantly reduced with regard to the number of main stem nodes, primary, secondary and tertiary branching, number of seed capsules produced and dry weight under UNR compared with WR. However, plant height was not affected by the management system. Spurge growth was significantly reduced with regard to branching and fresh weight but not height. These results show that UNR might suppress weed development by reducing vegetative and reproductive growth of prickly sida and vegetative growth of spurge. The reduction in seed and seed capsule production of prickly sida is likely to reduce its reproductive potential and also diminish the subsequent seed rain and soil seed bank reserves. Thus, a potential benefit of UNR cotton management systems might be to reduce the competitive ability of weeds and decrease seed production. 相似文献
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