The Effect of Detopping the Field Bean Plants (Vicia faba L. minor) and Lack of the Pod Setting on the Symbiosis with Root Nodule Bacteria     

W. Filek    F. Dubert  M. Kaminski 《Journal of Agronomy and Crop Science》1996,176(1):39-45

The plants of field bean var. Nadwiślaνski were detopped after about 1 month since the beginning of flowering and after that all flower buds were removed from some plants. The effect of a change in the trophic balance on the setting and subsequent growth of root nodules was studied.
The removal of pods had the greater effect on the increase in the weight of the remaining organs than the detopping of plants. Detopping retarded the ageing of the plants and delayed the phase of the greatest reduction of the number of pods. The detopping of plants revealed their considerable compensative possibilities in terms of production of the pod weight and the potential of leaves for biomass production. The data obtained indicate that plant detopping may disturb the root–bacteria exchange of signals, necessary to initiate the nodule development, while the further growth of nodules depends, above all, on the amount of available photosynthates.
A very high correlation between the dry weight of the whole plant to the dry weight of leaves ratio and the weight of nodules was observed. It proves the importance of the trophic balance of the plant for the root nodule weight production.  相似文献   

The Content of β-endorphin-like Immunoreactivity in Porcine Corpus Luteum and the Potential Roles of Progesterone, Oxytocin and Prolactin in the Regulation of β-endorphin Release from Luteal Cells in vitro     

J Przala  T Kaminski  S Okrasa  G Siawrys  & I Bogacka 《Reproduction in domestic animals》2001,36(2):107-112

The amount of β‐endorphin‐like immunoreactivity (β‐END‐LI) in porcine corpora lutea from several stages of the oestrous cycle and the effects of progesterone, oxytocin, and prolactin on β‐END‐LI secretion in vitro by luteal cells were studied. Porcine corpora lutea obtained on days 1–5, 6–10, 11–13, 14–18, and 19–21 of the cycle were used to prepare extracts for β‐END‐LI determination. Additionally, corpora lutea from days 11–13 and 14–18 were enzymatically dissociated and isolated luteal cells were used for further study of β‐endorphin secretion in vitro. Cells were cultured in serum‐free defined M 199 medium (10⁶ cells/ml) at 37°C under 5% CO₂ in air, for 12 h. The influences of the following factors on β‐END‐LI secretion by luteal cells were tested: progesterone (10^–9, 10^–7 and 10^–5M ), oxytocin (0.01, 0.1, 1 and 10 ng/ml), and prolactin (0.1, 1, 10 and 100 ng/ml). The β‐END‐LI contents in extracts and media were measured by radioimmunoassay. The tissue concentration of β‐END‐LI was lowest on days 1–5 of the cycle (0.35 ± 0.03 ng/g wet tissue). Subsequently, it constantly increased to the highest value on days 14–18 (16.58 ± 0.52 ng/g wet tissue) and on days 19–21 it declined (11.10 ± 0.52 ng/g wet tissue). Progesterone at a low dose (10^–9 M ) resulted in significant (p < 0.05) increases and decreases in β‐END‐LI secretion by luteal cells from days 11–13 and 14–18, respectively. Higher doses of progesterone (10^–7 and 10^–5 M ) had no effect on β‐END‐LI release, compared with the control group. All dose‐levels of oxytocin used decreased β‐END‐LI secretion by luteal cells on days 11–13 and 14–18 of the cycle. Prolactin at doses of 0.1 and 1 ng/ml on days 11–13, and all doses tested on days 14–18 resulted in decreases in β‐END‐LI release from luteal cells. These results document evident changes in β‐END‐LI content in the pig corpus luteum during its development and indicate the potential roles of progesterone, oxytocin, and prolactin in luteal cell secretion of β‐END‐LI.  相似文献   

The Influence of Opioid Peptides on Steroidogenesis in Porcine Granulosa Cells     

T Kaminski  G Siawrys  I Bogacka  S Okrasa  J Przala 《Reproduction in domestic animals》2004,39(1):25-32

The present studies were undertaken to examine the influence of mu (beta-endorphin, DAMGO, FK 33-824), delta (met-enkephalin, leu-enkephalin, DPLPE) and kappa opioid receptor agonists (dynorphin A, dynorphin B, U 50488) used at different doses (1-1000 nM) alone and in combination with LH (100 ng/ml) on steroidogenesis in porcine granulosa cells derived from large follicles. The effects of mu, delta and kappa receptor agonists on both basal and LH-induced progesterone (P4) secretion were negligible. Agonists of mu opioid receptors reduced basal androstenedione (A4), testosterone (T) and oestradiol (E2) release. Co-treatment with LH entirely abolished the inhibitory effect of these agonists on A4 and E2 secretion and resulted in an increase in T release. The addition of delta receptor agonists was followed by a decrease in basal A4, T and E2 secretion. The cells incubated in the presence of LH increased the androgen production and abrogated the inhibitory effect of delta agonists on E2 output. Basal A4, T and E2 release was also suppressed by kappa receptor agonists. The presence of LH in culture media extended the inhibitory effect of these opioids on E2 output and caused either abolition of the inhibitory influence of kappa agonists or even augmentation of both androgen release in response to the opioids. In conclusion, these data support the involvement of three major types of opioid receptors in the regulation of porcine granulosa cell steroidogenesis.  相似文献   

Influence of caudal epidural analgesia on cortisol concentrations and pain‐related behavioral responses in mares during and after ovariectomy via colpotomy          下载免费PDF全文

Aileen L. Rowland DVM  Kati G. Glass DVM  DACVS  Sicilia T. Grady DVM  PhD  Kevin J. Cummings DVM  PhD  Katrin Hinrichs DVM  PhD  DACT  Ashlee E. Watts DVM  PhD  DACVS 《Veterinary surgery : VS》2018,47(5):715-721

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Bacteraemia in 66 cats and antimicrobial susceptibility of the isolates (1995-2004)     

Greiner M  Wolf G  Hartmann K 《Journal of Feline Medicine and Surgery》2007,9(5):404-410

Bacterial blood culture results of 292 privately owned cats presented to the Clinic for Small Animal Medicine, Ludwig Maximilian University Munich with signs of sepsis were evaluated retrospectively. Of the blood cultures, 23% were positive. In 88%, a single bacterial species was isolated. Of all bacterial isolates, 45% were Gram-positive, 43% were Gram-negative, and 12% were obligate anaerobes. The most frequently isolated bacteria were Enterobacteriaceae, obligate anaerobic species, Staphylococcus species and Streptococcus species. Of the cats with positive blood cultures, 32% were pretreated with antibiotics. Of all bacterial isolates, 77% were susceptible to enrofloxacin, 69% to chloramphenicol, 67% to gentamicin, and 64% to amoxycillin clavulanic acid. Only enrofloxacin reached an in vitro efficacy of more than 70% against Gram-positive and more than 74% against Gram-negative bacteria.  相似文献   

Solitary intraventricular tumors in dogs and cats treated with radiotherapy alone or combined with ventriculoperitoneal shunts: A retrospective descriptive case series     

Katrin Beckmann  Malwina Kowalska  Valeria Meier 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):204-215

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作物生产给植物提供真正所需     

Katrin Rutt 《农业工程》2012,2(3):90-91

精准农业的目的就是要按照作物生长需要有针对性地进行栽培管理。该文从耕种方式、播种方式、施肥方式以及农田作业轨迹方面出发,介绍了现行一些精准农业发展情况,同时指出了所面临的问题及发展方向。   相似文献   

Glycoprotein M of bovine herpesvirus 1 (BHV-1) is nonessential for replication in cell culture and is involved in inhibition of bovine respiratory syncytial virus F protein induced syncytium formation in recombinant BHV-1 infected cells     

König P  Giesow K  Keil GM 《Veterinary microbiology》2002,86(1-2):37-49

Cell cultures infected with BHV-1/F(syn), a recombinant bovine herpesvirus 1 (BHV-1) which expresses a synthetic open reading frame encoding the fusion (F) protein of the bovine respiratory syncytial virus (BRSV), showed a cytopathic effect (CPE) indistinguishable from that induced by wildtype BHV-1 although transient transfection experiments demonstrated that expression of the F protein leads to formation of large syncytia. Since it has been shown that glycoprotein M (gM) of pseudorabies virus inhibits BRSV F-induced syncytium formation in transient plasmid transfection experiments [Pseudorbies virus glycoprotein M inhibits membrane fusion. J. Virol. 74 (2000) 6760], the gM ORF of wtBHV-1 and BHV-1/F(syn) was interrupted. Infection of cell cultures with the resulting gM(-) mutant of BHV-1/F(syn) led to formation of syncytia, whereas the CPE in gM(-)BHV-1 infected cells was comparable to the CPE in wtBHV-1 infected cultures. Our results demonstrate that gM is not essential for BHV-1 replication in cell culture and that gM is involved in inhibition of the cell fusion activity of the BHV-1 expressed BRSV F protein.  相似文献   

Goniodysgenesis associated with primary glaucoma in an adult European Short-haired cat     

Katrin Trost  Robert L. Peiffer Jr  †  Barbara Nell 《Veterinary ophthalmology》2007,10(S1):3-7

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Comparison of different diagnostic tools for the detection of Anaplasma phagocytophilum in dogs     

Charlotte Barth  Reinhard K. Straubinger  Elisabeth Müller  Carola Sauter‐Louis  Katrin Hartmann 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(2):180-184

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