Miniaturized methods for testing the toxicity of pesticides to microorganisms     

S. L. COOPER  G. I. WINGFIELD  R. La WLEY  M. P GREAVES 《Weed Research》1978,18(2):105-107

A minialurized method of determining the toxicity of pesticides to large numbers or soil mitroorganisms is described. Bacteria. actinomycetes, yeasts, fungi and algae can be used as test organisms. A modification of the method allows determination of minimum inhibitory concentrations of pesticides to specific microorganisms. Some results obtained with herbicides are presented. Microméthodex pour éprauver la toxicité des pesticides vis-á-vis des micro-organismes Une micromethode pour determiner la toxicité des pesticides vis-á-vis de nombreux micro-organismes du sol est décrite. Des bactéries. des actinomycetes, des levures. des champignons et des algues peuvent ëtre utilisés comme organismes-tesis. Une modifitation de la méthode permel les determinations des concentrations minimales inhibitrices des pesticides vis-á-vis des micro-organismes specifiques. Quelques resultats obtenus avec des herbicides sont présentés. Miniaturisierte Methoden zur Prüf'ung der Toxizitüt von Pflan- zenhehandlumgsmit teln auf Mikraorganismen Es wird einc miniaturisierte Methode zur BestimmungderToxi- zität von Pfianzenbehandlungsmitteln für groβe Zablen von Bodenmikroorganismen beschrieben, Als Testorganismen können Bakterien. Actinomyceten. Hefen, Pilze und Algen benutzi werden. Eine Modifizierung der Methode gestattet die Bestimmung der Minimum-Hemmkonzentration von Pflanzen- behandlungsmitteln auf spezielle Mikroorganismen. Einige Ergebnisse, die mit Herbiziden erarbeitel wurden. sind darge-stellt.  相似文献   

Soil CO2 emission in sugarcane management systems     

Rose Luiza Moraes Tavares  Zigomar Menezes de Souza  Diego Silva Siqueira  Newton La Scala Júnior  Alan Rodrigo Panosso 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(8):755-762

Sugarcane management systems affect soil attributes such as the carbon cycle. This fact has stimulated the sugar and alcohol industry to refine the sugarcane production systems by replacing the pre-harvest burning (PB) and manual harvest with mechanized harvesting followed by residue deposition. The aim of this study was to evaluate different management systems with respect to C cycling carbon dioxide and soil parameters (chemical, physical and biological) which were determined over the season. Three sugarcane cultivation systems were evaluated at the following periods: (a) PB, (b) 5-year green harvest and (c) 10-year green harvest. The results indicated that CO₂ emission was 36% greater in the 10-year sugarcane green harvest system than in the PB system. The bulk density and macroporosity were the factors that were most affected by the different sugarcane management systems and that significantly influenced soil CO₂ emissions. The principal component analysis showed that soil CO₂ emission was 18% influenced by base saturation (V%) and 14% by pH, especially in the PB area. Additionally, 19% was affected by carbon and macroporosity in the 5- and 10-year green harvest areas, respectively. From our results, it can be concluded that the most CO₂ emissions are in the areas of sugarcane green, this is due to the higher carbon concentration when compared with the area of burning sugarcane. The parameters that most influenced the CO₂ emissions were bulk density, porosity, macroporosity, pH and V%.  相似文献   

Milk yield, age at first calving, and the risk of BSE: an analysis at the farm level in France     

La Bonnardière C  Bonaiti B  Abrial D  Gasqui P  Calavas D  Ducrot C  Barnouin J 《Preventive veterinary medicine》2007,78(1):67-78

The epidemic of bovine spongiform encephalopathy (BSE) in France, as in the UK, has affected dairy cattle much more than beef cattle. However, the intensification of dairy herd management as a risk factor for BSE has not to date been analyzed. For this purpose, two databases were merged: the French Milk Records database, and the French BSE database, which can be considered as being devoid of notification bias since July 2001, when systematic tests were implemented. Only pure Holstein herds were considered, which represent the vast majority of total and BSE-affected dairy herds in France. A case-control study was designed so that 20 control herds were matched to each case herd according to the location of the farm and the year of birth of the index case. Three thousand and forty five farms were included, among which 145 with a BSE case notified between July 2001 and July 2003, and 2900 controls. With respect to the risk of BSE, odds ratios for each class of milk yield and age at first calving were estimated by using conditional logistic regression models with appropriate adjustments to herd size. The two main results were the following: firstly, whereas most Holstein herds, with average production between 7000 and 10,000kg, had nearly the same BSE risk, a small category of very intensive herds, with annual milk yields above 10,000kg, were significantly more at risk than the other herds. Secondly, a very early first calving (under 26 months of age) was found to be at risk for BSE as compared to other categories, independently of the milk yield. These results are discussed in the light of the known age-dependent susceptibility to BSE.  相似文献   

春小麦高产叶龄模式的研究     

吕凤山  毛敬国  刘宝昌 《灌溉排水学报》1995,(2)

本研究根据春小麦的叶龄进程，对水、肥、密等影响产量的主要因素进行优化组合，提出了旨在提高春小麦的产量及获得最佳效益的高产模式，为指导我国北方春小麦的生产提供了科学依据。  相似文献   

病原检测基因芯片应用及在水产病害检测的前景   总被引：6，自引：0，他引：6  

许拉  黄健  杨冰 《海洋水产研究》2008,29(1):109-114

基因芯片技术是近年来迅速发展起来的一项新技术,已成为国内外研究的热点。作为生物芯片技术发展中最完备的一个分支,基因芯片以其高通量、高灵敏性、高特异性的特点,在细菌、病毒、真菌等病原检测和鉴定方面发挥着越来越重要的作用。本文介绍了基因芯片的原理和作为检测手段的优越性,并综述了基因芯片技术在细菌和病毒检测方面的应用,分析了现有水产动物病原检测技术,提出了在现有水产动物病原生物信息学的基础上,研发水产养殖动物病害诊断基因芯片的策略。  相似文献   

小麦抗麦红吸浆虫品种遗传多样性的表型和SSR标记分析   总被引：2，自引：0，他引：2  

仇松英  史晓芳  史忠良  张松令  谢福来  高炜  逯腊虎 《麦类作物学报》2011,31(6):1050-1056

为更深入地揭示小麦抗麦红吸浆虫品种（系）的遗传多样性,从而为进一步选育抗虫品种提供依据,在对田间虫圃1 562份小麦品种（系）损失率鉴定的基础上,取47份年度间鉴定抗性结果较为一致的材料,利用表型和SSR标记,进行遗传多样性分析。这些抗麦红吸浆虫品种农艺性状表现出较大的差异,表型聚类在遗传距离为0.68处将供试材料分为6个类群。19对SSR标记在47份不同抗性品种中检测到104个等位基因,能够将所有品种区分开来,每对引物可以检测到3～8个等位基因,平均5.47个。47个小麦品种间遗传距离为0.40～0.95,平均为0.71。SSR标记聚类分析在遗传距离为0.74处将供试材料分为6大类群。Mental测验结果表明,表型同基因型距离矩阵间存在显著正相关（r=0.76,P〈0.05）。抗虫品种晋麦65号单独聚为一类,同其余品种具有较远的亲缘关系,可作为新的抗源用于抗虫育种,并在吸浆虫发生地块推广种植。  相似文献   

西藏农村远程教育与信息服务体系发展对策研究     

黄界  牛磊  唐广元  杨勇  扎西拉宗  拉琼 《计算机与农业》2010,(1):37-41

西藏农村远程教育与信息服务体系是实现西藏农牧业跨越式和可持续发展、增加农牧民收入和促进农村经济、文化快速发展的重要平台。在基于西藏农牧业教育、农村信息化建设的现状的条件下,通过对农村远程教育与信息化服务所面临的主要问题的分析,提出了西藏农村远程教育与信息服务体系发展的对策。  相似文献   

病原微生物药敏检测方法的研究进展     

刁菁  杨秀生  李天保  叶海斌  许拉  樊英  王淑娴 《中国农学通报》2013,29(8)

抗生素敏感试验在当前病原微生物耐药性持续增长的形势下,已成为指导临床科学合理用药不可或缺的重要环节.因此,药敏检测方法的完善与发展是快速获取科学、准确微生物耐药谱的前提,也是实现有效临床治疗的重要保障.本研究介绍了当前应用于微生物药物敏感检测的常规方法,如纸片扩散法、稀释法、Etest法等,简要分析了它们之间的优缺特性,并对近年来一些新兴发展的快速药敏检测方法进行了总结以及展望了不同类型药敏检测方法的发展和应用趋势.  相似文献   

弯扭耦合柔性构件系统的变结构控制研究     

吕永桂  陈凯  魏燕定 《农业机械学报》2010,41(9):199-204

对由柔性杆和柔性梁组成的两连杆柔性构件系统的弯扭耦合振动进行了研究,建立了此柔性系统的动力学方程。在柔性杆根部粘贴一只压电扭转致动器抑制柔性杆的扭转振动,在柔性梁根部粘贴一只压电弯曲致动器抑制柔性梁的弯曲振动。采用一种带有饱和环节及滤波器的滑模变结构控制策略,进行了实验研究。结果表明：施控后的系统是稳定的,弯扭的各阶模态均能得到有效抑制,柔性梁末端的振动位移能得到显著衰减。  相似文献   

农田防护林更新树种选择及配置模式研究   总被引：5，自引：0，他引：5  

赵克昌  周腊虎 《防护林科技》2002,(3):14-17

对农田防护林更新树种和配置模式进行了分析研究。从新品种的引种上筛选出适于河西走廊发展的农田防护林树种为三倍体毛白杨B10 0 8和BT85 ,并能在瘠薄和盐碱化土壤上良好生长 ;经抗寒性分析得出垂柳、云杉、白榆、柽柳、沙枣抗冻性较强 ;为减缓天牛等蛀干害虫危害 ,在林网配置模式上引入了云杉、侧柏、樟子松、垂柳、小叶白蜡、国槐等耐瘠薄抗寒性强的树种 ,营造多树种林带 ,组成混交林网结构 ,使林网中感虫杨树的比例占 2 0 %左右 ,增加了林网结构的稳定性和长效性  相似文献