Identification and characterization of two phytoplasma subgroups (16SrXI-D and 16SrXIV-A) associated with lychee (Litchi chinensis Sonn) in India     

Rao  Govind P.  Tiwari  Ajay K.  Madhupriya  Dubey  Durgesh 《植物病害和植物保护杂志》2017,124(3):235-239

Journal of Plant Diseases and Protection - Leaf rolling, necrosis and apical bud proliferation symptoms were recorded on lychee plants at Bhatpar Rani (Deoria district) and Gorakhpur (Gorakhpur...  相似文献   

Association of different groups of phytoplasma in flower malformation,phyllody, foliar yellowing,and little leaf disease of rose (Rosa sp.)     

Madhupriya  Namita Banyal  Raju Dantuluri  V.S.  G. P. Rao  S. M. Paul Khurana 《The Journal of Horticultural Science and Biotechnology》2017,92(4):424-431

An extensive survey on rose cultivars in New Delhi showed phytoplasma-suspected symptoms such as leaf chlorosis, phyllody, virescence, and little leaf in 35 rose cultivars. Vegetative propagation through stem and tissue culture was performed. The cuttings/budded plants raised from infected plants showed the symptoms of phytoplasma disease. Phytoplasma was detected in 13 symptomatic plants using nested polymerase chain reaction (PCR) with phytoplasma-specific primer pairs P1/P7 and R16F2n/R16R2. Nested PCR amplicons from the 13 most severely infected rose cultivars (Arunima, Sindoor, Taboo, Sunsation, Raktgandha, Jawahar, Surkhab, Pusa arun, Marco Polo, Perfume de French Comete, Green Rose, Snowfire, and Gladiator) were cloned and sequenced. BLASTn analysis of 16S rRNA gene sequences, virtual RFLP and phylogenetic analysis revealed that the phytoplasma sequences amplified from rose belonged to ‘Candidatus Phytoplasma asteris’ subgroup 16SrI-B and ‘Ca. P. australasia’ subgroup 16Sr II-D. Our results suggest the inclusion of phytosanitary measures in planting materials for management of these phytoplasma diseases.  相似文献   

Identification of sugarcane grassy shoot-associated phytoplasma and one of its putative vectors in India     

Govind Pratap Rao  Madhupriya  Ajay Kumar Tiwari  Shailendra Kumar  Virendra Kumar Baranwal 《Phytoparasitica》2014,42(3):349-354

During a survey of sugarcane fields at the Sugarcane Research Institute, Shahjahanpur, Uttar Pradesh, India, in August–September 2012, 6% to 28% incidence of sugarcane grassy shoot disease was observed in different fields of sugarcane variety CoS 7250. The association of phytoplasma with symptomatic sugarcane was confirmed by direct and nested PCR amplification of phytoplasma ribosomal gene. Four different delphacid leafhopper species, viz. Cofana unimaculata Signoret, Exitianus indicus (Distant), Sogatella kolophon Kirkaldy and Hishimonus phycitis (Dist.) were the prevalent feeding species of the Auchenorrhyncha fauna in the symptomatic sugarcane fields. Out of these four leafhopper species, only E. indicus tested positive for phytoplasma presence. Phylogenetic analysis suggested that the phytoplasmas from sugarcane and E. indicus in the present study were members of 16Sr XI. The confirmation of association of sugarcane grassy shoot phytoplasma in E. indicus population is important to understand the secondary spread of this phytoplasma in sugarcane plants.  相似文献