Combined treatments of spinosad and chlorpyrifos-methyl for management of resistant psocid pests (Psocoptera: Liposcelididae) of stored grain     

Nayak MK  Daglish GJ 《Pest management science》2007,63(1):104-109

The combined efficacy of spinosad and chlorpyrifos-methyl was determined against four storage psocid pests belonging to genus Liposcelis. This research was undertaken because of the increasing importance of these psocids in stored grain and the problem of finding grain protectants to control resistant strains. Firstly, mortality and reproduction were determined for adults exposed to wheat freshly treated with either spinosad (0.5 and 1 mg kg(-1)) or chlorpyrifos-methyl (2.5, 5 and 10 mg kg(-1)) or combinations of spinosad and chlorpyrifos-methyl at 30 degrees C and 70% RH. There were significant effects of application rate of spinosad and chlorpyrifos-methyl, both individually and in combination, on adult mortality and progeny reduction of all four psocids. Liposcelis bostrychophila Badonnel and L. decolor (Pearman) responded similarly, with incomplete control of adults and progeny at both doses of spinosad but complete control in all chlorpyrifos-methyl and combined treatments. In L. entomophila (Enderlein) and L. paeta Pearman, however, complete control of adults and progeny was only achieved in the combined treatments, with the exception of spinosad 0.5 mg kg(-1) plus chlorpyrifos-methyl 2.5 mg kg(-1) against L. entomophila. Next, combinations of spinosad (0.5 and 1 mg kg(-1)) and chlorpyrifos-methyl (2.5, 5 and 10 mg kg(-1)) in bioassays after 0, 1.5 and 3 months storage of treated wheat were evaluated. The best treatment was 1 mg kg(-1) of spinosad plus 10 mg kg(-1) of chlorpyrifos-methyl, providing up to 3 months of protection against infestations of all four Liposcelis spp. on wheat.  相似文献   

Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India     

Richa Sood  Rekha Khandia  Sandeep Bhatia  Divakar Hemadri  Manoj Kumar  Sharan S. Patil  Atul K. Pateriya  Arshi Siddiqui  Malkanna Sanjeev Kumar  Mudalagiri Dasappa Venkatesha  Diwakar D. Kulkarni 《Tropical animal health and production》2014,46(6):1037-1043

Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world.  相似文献   

Effect of indaziflam on microbial activity and nitrogen cycling processes in an orchard soil     

Amir M. GONZ&#;LEZ-DELGADO  Pierre-Andr&#; JACINTHE  Manoj K. SHUKLA 《土壤圈》2022,32(6):803-811

Indaziflam is a preemergent herbicide widely used for the control of weeds in pecan (Carya illinoinensis) orchards in the southwestern region of the United States. Given the paucity of data regarding the effect of indaziflam on the biochemical properties of soils supporting pecan production, this study was conducted to evaluate the effects of different application rates of indaziflam on soil microbial activity, diversity, and biochemical processes related to nitrogen (N) cycling. During two consecutive growing seasons (2015 and 2016), soil samples were obtained from experimental mesocosms consisting of soil-filled pots where pecan saplings were grown and treated with indaziflam applied at two different rates (25 and 50 g active ingredient (ai) ha^-1, with the higher rate being slightly lower than the recommended field application rate of 73.1 g ai ha^-1). Soil samples were collected approximately one week before and one week after herbicide application for determination of soil microbial biomass and diversity, N mineralization, and β-glucosaminidase activity. Soil samples collected from the control mesocosms without herbicide application were treated in the laboratory with two rates of indaziflam (75 and 150 g ai ha^-1) to determine the immediate effect on microbial activity. No significant effect of herbicide treatment on soil respiration and microbial biomass was detected. The results showed a slight to moderate decrease in microbial diversity (7% in 2015 and 44% in 2016). However, decreased β-glucosaminidase activity with herbicide treatment was observed in soils from the mesocosms (33%) and soils treated with indaziflam in the laboratory (45%). The mineral N pool was generally dominated by ammonium after indaziflam application, which was consistent with the drastic decrease (75%) in nitrification activity measured in the laboratory experiment. The results of this study indicate that indaziflam, even when applied at higher than recommended rates, has limited effects on soil microbial activity, but may affect N cycling processes.  相似文献   

Geomorphology, pedology and sedimentology of the Deoha/Ganga–Ghaghara Interfluve, Upper Gangetic Plains (Himalayan Foreland Basin) — Extensional tectonic implications     

Satvindar Singh  B. Parkash  M.S. Rao  Manoj Arora  Balaji Bhosle   《CATENA》2006,67(3):148-203

Regional mapping of soils helped to identify and map 24 soil-geomorphic units on the Deoha/Ganga–Ghaghara Interfluve in the Upper Gangetic Plains (Himalayan Foreland Basin). On the basis of luminescence ages soil-geomorphic units can be grouped into five members of a Morphostratigraphic Sequence with ages of ≤ 1.7 ka, 1.8–3.6 ka, 4.6–6.4 ka, 6.8–10 ka and > 10 ka. Except for two units with sandy parent material, all the members with loamy parent materials show systematic increase in the degree of soil development from Member QGMS-I to V. Major pedogenic processes are salinization, alkalinization, illuviation, calcrete development and gleying, and degradation of some micromorphological features is observed in the oldest soils.Regional mapping and dating of soils show that the Interfluve between the Deoha/Ganga–Ghaghara rivers is bounded by longitudinal faults, along the bounding rivers. The major longitudinal faults trend N–S or NNE–SSW in the northern region; turn N–S in the central region and take easterly to SEE direction in the south, giving the Interfluve a curvilinear shape. Strike of a set of six transverse extensional normal faults changes from approximately E–W to NEE–SWW in the southernmost region of the Interfluve. Downthrown sides are to the south and to the east in northern region and western region, respectively.Due to the activity of different segments of various transverse faults during a probably dry sub-humid to semi-arid climatic period of 10–5 ka, terminal fans were deposited on the downthrown blocks. Thus, role of extensional tectonics in an overall compressional regime is significant. Also, tilting of smaller blocks leading to the shifting away of large rivers seems to be an additional control on the distribution of soils and sedimentation on the Interfluve.  相似文献   

Plant defense activation and management of tomato root rot by a chitin-fortified <Emphasis Type="Italic">Trichoderma/Hypocrea</Emphasis> formulation     

Manoj Kumar Solanki  Nidhi Singh  Rajesh Kumar Singh  Pratiksha Singh  Alok K. Srivastava  Sudheer Kumar  Prem L. Kashyap  Dilip K. Arora 《Phytoparasitica》2011,39(5):471-481

Tomato root rot caused by Rhizoctonia solani is a major soilborne disease resulting in significant yield loss. The culture filtrates of six isolates of Trichoderma/Hypocrea species were evaluated for in vitro production of hydrolytic enzymes. Results demonstrated that all the six isolates were able to produce chitinase, β-1, 3 glucanase and protease in the range of 76–235 μmol GlcNAc min^-1 mg^-1 protein, 31.90–37.72 nmol glucose min^-1 mg^-1 proteins and 63.05–86.22 μmol min^-1 mg^-1 proteins, respectively. Trichoderma/Hypocrea-based formulation(s) were prepared with chitin (1% v:v) and CMC (0.5% w:v) for root rot management in a greenhouse. Root dip application with bioformulation(s) resulted in a significant reduction of the root rot index. In addition, bioformulations increased plant growth attributing traits significantly relative to untreated control. Accumulation of total phenols, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase increased in chitin-supplemented Trichoderma/Hypocrea formulation-treated plants challenged with R. solani. The results suggest that chitin-fortified bioformulation(s) could be an effective system to control root rot of tomato in an eco-compatible manner.  相似文献   

Increase in Growth,Productivity and Nutritional Status of Wheat (Triticum aestivum L.) and enrichment in Soil Microbial Population Applied with Biofertilizers Entrapped with Organic Matrix     

Manoj Kumar  Kuldeep Bauddh  Manish Sainger  Poonam Ahlawat Sainger 《Journal of plant nutrition》2015,38(2):260-276

To find effective alternatives to reduce the application of conventional urea (CU), a conventional biofertilizer (CB) preparation (charcoal mixed Azotobacter chroococcum and Bacillus subtilis) and the same biofertilizers entrapped in an organic matrix consisting of cow dung, rice bran, dried powder of neem leaves, and clay soil in 1:1:1:1 ratio and 25% (w/w) saresh (plant gum of Acacia sp.), named as super granules of biofertilizers (SGBF) were applied to cultivate wheat (Triticum aestivum L. cv. ‘WH-711’) in experimental plots. The results revealed that the efficacy of commercially available charcoal mixed biofertilizers could not prove as effective alternative to CU, whereas the same dose of biofertilizers entrapped in the organic matrix, SGBF, resulted in a significant increase in growth and productivity of wheat. It appears that SGBF prepared and applied in this study is an effective organic alternative to the urea for wheat cultivation in semi-arid subtropical agro-ecosystems.  相似文献   

The transcriptome of the sea urchin embryo     

Samanta MP  Tongprasit W  Istrail S  Cameron RA  Tu Q  Davidson EH  Stolc V 《Science (New York, N.Y.)》2006,314(5801):960-962

  相似文献   

甘蔗ATP柠檬酸裂解酶基因的克隆与表达分析     

李长宁  农倩  谭秦亮  杨丽涛*  李杨瑞* 《作物学报》2012,38(11):2024-2033

ATP柠檬酸裂解酶(ACL)为细胞质中乙酰辅酶A合成途径的关键调控酶,在生物体正常生长发育中扮演着重要角色。本研究通过Race和电子克隆技术获得编码甘蔗ACL蛋白2个亚基的基因SoACLA-1和SoACLB-1,其编码框长度分别为1 272 bp和1 827 bp,编码423个和608个氨基酸,推测的氨基酸序列与其他物种具有高度相似性,都优先与禾本科植物聚于同一进化分支。2个基因在ATP-grasp功能域、柠檬酸结合位点、组氨酸磷酸化位点和ATP结合、CoA结合、磷酸化区域等,序列高度保守。实时荧光定量PCR结果表明,2个基因均受外源ABA、水分胁迫、水分胁迫加ABA处理诱导表达,且叶中表达量显著高于根系,其中又以水分胁迫处理下的表达量最高,2个基因表现出协同表达模式。SoACLA-1和SoACLB-1的表达与ABA、ROS含量具有相关性,说明它们可能参与了ABA调控的植物对逆境胁迫反应的代谢过程。  相似文献   

Increase in growth,productivity and nutritional status of rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L. cv. Basmati) and enrichment in soil fertility applied with an organic matrix entrapped urea     

Manoj Kumar  Kuldeep Bauddh  Manish Sainger  Poonam Ahlawat Sainger  Jay Shankar Singh  Rana Pratap Singh 《Journal of Crop Science and Biotechnology》2012,15(2):137-144

Field experiments were conducted to evaluate the effects of eco-friendly organic matrix entrapped urea (OMEU) on growth, productivity, and yield of rice (Oryza sativa L. cv. Basmati) and soil enrichment in the paddy field at Rohtak (Haryana) located near Delhi. The OMEU prepared in granular form contained cow dung, rice bran (grain cover of Oryza sativa), powder of neem leaves (Azadirachta indica), and clay soil (diameter of particles < 0.02 mm) in 1:1:1:1 ratios and saresh (plant gum of Acacia sp.) as binder along with half of the recommended dose of commercially available soluble urea (free urea; FU). Single basal application of OMEU showed an increase in plant growth in terms of fresh and dry weights, root length, root, leaf and tiller numbers, soluble protein, total N and ammonium in leaves, productivity in terms of grain and straw yield, and nutritional and microbial activities of field soil over free form of urea and no fertilizer application. Nutritional status of rice grains was also improved over the free urea and no fertilizer controls. Our data indicate that OMEU, which is low cost and based on bio-degradable, non-toxic, and locally available agro-waste, can be attempted to replace the conventional use of soluble urea in rice.  相似文献   

Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]     

Sarika Gupta  Kajal Kumari  Mehanathan Muthamilarasan  Alagesan Subramanian  Manoj Prasad 《Plant Breeding》2013,132(4):367-374

Although the foxtail millet [Setaria italica (L.) P. Beauv.] is recently regarded as a model crop for studying functional genomics of biofuel grasses, its genetic improvement to some extent was limited due to the non‐availability of molecular markers, particularly the microsatellite markers and the saturated genetic linkage map. Considering this, we attempted to generate a significant number of microsatellite markers in cultivar ‘Prasad’. Two hundred and fifty‐six clones were sequenced to generate 41.82‐kb high‐quality sequences retrieved from genomic library enriched with dinucleotide repeat motifs. Microsatellites were identified in 194 (76%) of the 256 positive clones, and 64 primer pairs (pp) were successfully designed from 95 (49%) unique SSR‐containing clones. The 67.4% primer designing ability, 100% PCR amplification efficiency and 45.3% polymorphic potential in the parents of F₂ mapping population established the efficacy of genomic microsatellites. All the 64 microsatellite markers displayed high level of cross‐species amplification (~67%) in 10 millets and non‐millets species. These experimental findings suggest the utility and efficacy of SSRs in diverse genotyping applications, resolving QTLs, phylogenetic relationships and transferability in several important grass species.  相似文献