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1.
The polygalacturonases (PG) and oxalic acid produced by Sclerotinia sclerotiorum in infected soybean hypocotyls were investigated as elicitors of the phytoalexin glyceollin I.Purification to homogeneity through isoelectrofocusing and ion-exchange fast protein liquid chromatography revealed three endo-PG isoenzymes (PG-I, PG-II and PG-IV) and one exo-PG (PG-III) in 6-day-old etiolated soybean hypocotyls infected with the B-24 isolate of S. sclerotiorum.PG-I and PG-III, in the range of concentrations tested (0·15–1·2 reducing units ml−1), did not act as elicitors of glyceollin I synthesis. Some elicitor activity was shown by PG-II at 0·6–1·2 reducing units ml−1. PG-IV, at lower doses (0·038–0·30 reducing units ml−1), was even more effective in inducing phytoalexin synthesis. However higher concentrations of PG-IV induced tissue softening and decreased phytoalexin accumulation.PG-II and PG-IV released heat-stable elicitors from purified soybean cell walls supporting the evidence that uronides are intermediate inducers in elicitation by endo-PGs. Oxalic acid was an active elicitor of glyceollin I over the range of concentrations tested (0·31–20 m
) with the maximum at a concentration of 5 m
. The inability of oxalic acid to release uronides from purified cell walls makes it unlikely that uronide intermediate elicitors are involved in elicitation by oxalic acid. 相似文献
2.
Glyceollin I was fungistatic rather than fungicidal towardSclerotinia sclerotiorum. Within the mycelial mat apical cells were more vulnerable than mature cells.S. sclerotiorum removed large amounts of glyceollin from solution by a non-energy-requiring process.
Contribution from Ministero dell’Università e della Ricerca Scientifica e Tecnologica. 相似文献
3.
Paola De Benedictis Cristian De Battisti Sabrina Marciano Franco Mutinelli Ilaria Capua Giovanni Cattoli 《Veterinary journal (London, England : 1997)》2013,195(3):388-390
Replication competent vaccines have been used successfully for the control of terrestrial rabies, mainly in wildlife; however, these vaccine strains occasionally may induce rabies. In this study, a pyrosequencing protocol for the rapid identification of vaccine-associated rabies viruses was applied to the 2008–2011 Italian epidemic. There was no evidence of vaccine-associated rabies cases following oral vaccination of foxes with the SAG2 and SADB19 vaccine strains. 相似文献
4.
Marcio Rocha Francelino Carlos Ernesto G.R. Schaefer Felipe Nogueira Bello Simas Elpídio Incio Fernandes Filho Jos Joo Lelis Leal de Souza Liovando Marciano da Costa 《CATENA》2011,85(3):194-204
The main pedological, geomorphological and cryogenic features of Keller Peninsula, part of Admiralty Bay, King George Island, Maritime Antarctica, were mapped and quantified with emphasis on the relationship between the ice retreat process, melt-out, landform development and soil distribution. Moraines, protalus, scree slopes, inactive glacial cirques, uplift marine terraces, biogenic landforms, arêtes and Felsenmeer were mapped. Scree slope is the main landform, covering approximately 25% of the peninsula, indicating prominent paraglacial features. Inherited, glacial landforms, such as lateral moraines, highland plateau and exhumed U shaped-valleys, are now being exposed in north Keller by ice shrinkage of former ice protecting cover. Landforms influenced soil formation and stability. Cryosols and Leptosols (WRB) roughly corresponding to Gelisols and Entisols (SSS), respectively, are the most common soil classes, with an overall tendency of absent permafrost in the coastal areas, changing to sporadic permafrost at mid-slope, and discontinuous permafrost with greater altitude and substrate stability. 相似文献
5.
Salvador Becerra-Rodríguez Víctor Manuel Medina-Urrutia Marciano Manuel Robles-González Timothy Williams 《Euphytica》2008,164(1):27-36
Grapefruit growers in the tropics require information about existing and new citrus cultivars with high productivity potential.
The objective of this study was to determine the growth, yield, and fruit quality performance of seven pigmented and four
white grapefruit cultivars under the dry tropic conditions of Colima, Mexico. The trees were budded on sour orange (Citrus aurantium L.) rootstock and planted at a distance of 8 × 4 m. ‘Oroblanco’ and ‘Marsh Gardner’ white-fleshed grapefruit cultivars and
‘Chandler’, a pink-fleshed pummelo, were the largest trees with the greatest height (5.0–5.6 m), canopy diameter (6.2–6.3 m),
trunk diameter (21.9–23.3 cm), and canopy volume (109–123 m3). Lower height (4.3–4.8 m) and canopy volume (73–96 m3), but with similar canopy diameter to the previously mentioned cultivars, were recorded for the remaining pigmented cultivars.
‘Chandler’ pummelo and four pigmented grapefruit cultivars (‘Shambar’, ‘Río Red’, ‘Ray Ruby’, and ‘Redblush #3’) had yearly
productions of 34.8, 34.9, 34.1, 32.7, and 30.6 ton ha−1, respectively. The most productive white grapefruit cultivar was ‘Marsh Gardner’ (30.5 ton ha−1). Grapefruit cultivars having the largest fruit size showed a higher inverse relationship between fruit weight and yield
than those with small fruit. Most genotypes had higher values of fruit weight, juice content, and maturity index than those
required by the local market. The most promising grapefruit cultivars based on their acceptable growth, yield superior to
30 ton ha−1, and acceptable fruit color were ‘Río Red’, ‘Shambar’, ‘Ray Ruby’, and ‘Redblush #3’. 相似文献
6.
7.
P. Marciano 《Phytoparasitica》1994,22(2):101-108
The polygalacturonase (PG) activity in culture filtrates fromSclerotinia sclerotiorum is reduced when glyceollin I, the major soybean phytoalexin, is present in the culture medium. When the enzyme activities
in the culture filtrates are expressed per unit of fungal growth, PG activity decreases with increasing concentration of glyceollin
I in the culture medium. The phytoalexin does not influence the isoenzyme pattern. This suggests that glyceollin I may inhibit
quantitative but not qualitative enzyme production. Only the highest glyceollin I concentration tested inhibits oxalic acid
production. The inhibitory effect on mycelial growth is confirmed. The data suggest a further hypothesis about the role of
phytoalexin during pathogenesis. 相似文献
8.
Tegenes Senna de Oliveira Liovando Marciano de Costa Carlos Ernesto Schaefer 《Soil & Tillage Research》2005,83(2):260-269
Widespread intensive land use in the seasonal tropics can damage the physical stability of aggregates. Similar damage can be expected from wetting and drying cycles causing aggregate fragmentation and, consequently, leading to an increase in their specific area and exposure of internal electric charges. Thus, we hypothetised that the influence of wetting and drying cycles is dependent on the mineralogical composition of oxisols (latosols) and it is higher in soils with low aggregate stability. A greenhouse experiment was carried out to test this hypothesis in highly weathered soils from Brazil, all with variable-charge clays and highly stable aggregates. Wetting and drying cycles were defined from the quantity of water available between field capacity and the permanent wilting point. Soil columns were submitted to 0, 2, 6, 9, 12, 15 and 18 wetting and drying cycles. After each number of wetting and drying defined physical and chemical properties were determined. Statistical analysis, such as simple and multiple linear regression and Pearson's correlation were performed, showing significantly correlated WDC contents with wetting and drying cycles. The obtained results led to the conclusion that there was a close interdependence among mineralogical composition, aggregate stability and WDC influenced by wetting and drying cycles. Soils of reduced aggregate stability like kaolinitcs made them more susceptible to the action of wetting and drying on the WDC. Changes in the WDC with wetting and drying cycles showed correlated with eletrochemical properties. 相似文献
9.
The effects ofSclerotinia sclerotiorum live and autoclaved sclerotia, and sclerotial exudates, and commercial oxalic acid were testedin vitro on sevenConiothyrium minitans strains differing in aggressiveness towardsS. sclerotiorum. Only sclerotial exudates and autoclaved sclerotia affected the mycelial growth rate of almost all the strains tested, whereas
a change in theC. minitans mycelial growth pattern was observed in the presence of autoclaved sclerotia and live sclerotia germinating by the myceliogenic
eruptive germination. In addition, sclerotial exudates had a stimulatory effect on spore germination. These findings indicate
that the various treatments could influence theC. minitans strains regardless of their aggressiveness. 相似文献
10.
Tiago W.P. Mineo Adriano O.T. CarrascoJosé A. Marciano Karin WertherAramis A. Pinto Rosangela Z. Machado 《Veterinary parasitology》2009
Neospora caninum infections in chickens have been recently described by epidemiological and experimental approaches, and these birds may be considered natural intermediate hosts of the parasite. It has been postulated that other bird species might perform this role in wildlife as well. To better understand the sylvatic life cycle of N. caninum, further studies are required. In that sense, this work aimed to observe infection kinetics in pigeons experimentally infected with N. caninum. Experimental infections were conducted in parallel with a related protozoan, Toxoplasma gondii, which has been already described as able to infect pigeons in nature. Our results demonstrated that N. caninum disseminated through various tissues of this host and induced parasite-specific IgG seroconversion. Infection parameters were similar to that observed in the T. gondii infected group, although N. caninum-infected pigeons presented lower IgG titers during acute phase. The results herein described demonstrate that pigeons are a suitable model for N. caninum infection, considering that these data are in agreement with those observed in chickens experimentally infected with this parasite. As pigeons may be revealed as important reservoirs for N. caninum infection in nature, future studies are necessary to determine the real prevalence of this parasite in this and other birds in wildlife. 相似文献