Use of DNA array to screen blastocyst genes potentially involved in the process of murine implantation     

Qin J  Takahashi Y  Imai M  Yamamoto S  Takakura K  Noda Y  Imakawa K 《The Journal of reproduction and development》2003,49(6):473-484

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Sericin Accelerates the Production of Hyaluronan and Decreases the Incidence of Polyspermy Fertilization in Bovine Oocytes During In Vitro Maturation     

Misa HOSOE  Nao YOSHIDA  Yutaka HASHIYADA  Hidetoshi TERAMOTO  Toru TAKAHASHI  Sueo NIIMURA 《The Journal of reproduction and development》2014,60(4):268-273

Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%, 0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine oocytes.  相似文献   

Identification of hypothalamic genes in associating with food intake during incubation behavior in domestic chicken     

Misa Takeda  Takeshi Ohkubo 《Animal Science Journal》2019,90(9):1293-1302

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Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY)   总被引：1，自引：0，他引：1  

Qiaochun Wang  Yong Liu  Yonghong Xie  Misa You 《Potato Research》2006,49(2):119-129

Viral diseases constitute a major constraint to high yield and high quality production of potato. Potato leafroll virus (PLRV) and Potato virus Y (PVY) are among the most damaging potato viruses and are prevalent in most potato growing areas. In the present study, attempts were made to eliminate PLRV and PVY by three cryogenic protocols, i.e., encapsulation-dehydration, encapsulation-vitrification and droplet. Results showed that both PLRV and PVY could be efficiently eliminated by cryogenic treatments with 83–86% and 91–95% of frequencies of virus-free plantlets obtained for the former and latter, respectively. Frequencies of virus-free plantlets produced by cryogenic treatments were higher than those by meristem culture (56% for PLRV and 62% for PVY) and thermotherapy (50% for PLRV and 65% for PVY), and similar to those by thermotherapy followed by meristem culture (90% for PLRV and 93% for PVY). Survival (75–85%) and regrowth (83–89%) from cryo-treated shoot tips were higher than those from meristem culture (50–55%) and thermotherapy followed by meristem culture (40–50%), but similar to those from thermotherapy (80–87%). The morphology of the plantlets regenerated from cryo-treated shoot tips was similar to that of non-treated plantlets. Thus, cryotherapy would provide an alternative method for efficient elimination of potato viruses, and can be simultaneously used for long-term storage of potato germplasm and for production of virus-free plants.  相似文献   

Gene expression profiling in embryonic chicken ovary during asymmetric development          下载免费PDF全文

Amir Hossan Shaikat  Shoko Namekawa  Sadequllah Ahmadi  Misa Takeda  Takeshi Ohkubo 《Animal Science Journal》2018,89(4):688-694

The reproductive system in female birds arises as bilateral asymmetrical anlagen, excluding the birds of prey. Earlier, histological and messenger RNA (mRNA) expression profile studies of several genes related to gonadal sex differentiation in chicken embryos tried to elucidate the query of this asymmetry in a scattered manner. To understand the matter precisely, we have focused on mRNA expression of a cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) in second half of the embryonic days (E10–E18). The established role of leptin in development of the embryo and its expression in the embryonic ovary also drove us to check leptin receptor (LEPR) expression in the ovary. Increased expression of FSHR and CYP19A1 in the left ovary compared with that in the right ovary was identified (P < 0.05), promoting preferential left ovarian development and functionality. Significant high expression (P < 0.05) of the apoptotic genes in the right ovary were also involved here. Leptin probably has no direct influence on ovarian asymmetry as no significant variation in gonadal mRNA expression of LEPR was observed within the same experimental days. We propose that asymmetric expression of this cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) leads to the development of dimorphic gonads during embryogenesis.  相似文献   

Development of DNA markers that discriminate between white- and blue-flowers in Japanese gentian plants     

Takashi?Nakatsuka  Misa?Saito  Yuka?Sato-Ushiku  Eri?Yamada  Takashi?Nakasato  Nobue?Hoshi  Kazumichi?Fujiwara  Takashi?Hikage  Masahiro?NishiharaEmail author 《Euphytica》2012,184(3):335-344

We developed molecular markers for discrimination of white and blue flower color in Japanese gentian plants. White-flowered gentians can be classified into two types, based on genetic and physiological features. One type includes four allelic variations (gtmyb3-1, gtmyb3-2, gtmyb3-3, and gtmyb3-4) of an anthocyanin biosynthetic regulator gene (GtMYB3), distinguished by three PCR-based molecular markers. The other type contains a newly identified inactive allele (ans1) of the anthocyanidin synthase (ANS) gene with a premature stop codon generated from a 4-bp deletion in the second exon. The ans1 allele was distinguished from the active ANS allele by a cleaved amplified polymorphism sequence (CAPS) marker. The genotypes of 12 white-flowered gentian cultivars/lines could be identified and classified as either ans1 or gtmyb3 using these four molecular markers. No white-flowered gentians contained ans1 and gtmyb3 alleles simultaneously. The mutated ANS gene co-segregated with white flower color in an F₂ population, demonstrating that the CAPS marker is useful to discriminate between white and blue flowers in gentian. Markers to discriminate flower color in Japanese gentian will be useful for early selection of progeny and for breeding management.  相似文献   

Steroidal but not embryonic regulation of mucin 1 expression in bovine endometrium     

Kaiyu KUBOTA  Masafumi MIWA  Ken-Go HAYASHI  Misa HOSOE  Miki SAKATANI 《The Journal of reproduction and development》2021,67(6):386

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Estimation of the assimilative capacity of fish-farm environments based on the current velocity measured by plaster balls     

Hisashi Yokoyama  Misa Inoue  Katsuyuki Abo 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):233-247

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Expression of monocarboxylate transporter 1 (MCT1) in the dog intestine     

Shimoyama Y  Kirat D  Akihara Y  Kawasako K  Komine M  Hirayama K  Matsuda K  Okamoto M  Iwano H  Kato S  Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):599-604

In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine.  相似文献   

Land Use After Forestland Allocation and the Potential for Farm Forestry in a Mountainous Region of Northeast Vietnam     

Thu Thuy Nguyen  Misa Masuda 《Small-Scale Forestry》2018,17(4):485-503

As a response to serious deforestation in Vietnam, the forestland allocation (FLA) policy was introduced in the 1990s. The process still continues, having allocated 80% of the forestland to various management entities, among which individual households are the most important one for production forests. While previous studies focusing on local livelihoods reveal negative impacts of the FLA, particularly in the initial stage, studies on forest cover change as well as government statistics indicate positive changes in the longer term. We focused on micro-scale land use change after the FLA, and selected two upland villages with different accessibility in the Northeast region of Vietnam. Though the FLA was initiated in 1995 in both villages, the government reforestation program first reached Village G with paved road, and they did second planting. However, forestland recipients in Village A, with no paved road, also planted timber species as soon as the program was introduced. Though the mean allocated areas in Village A was larger than that of Village G, the mean of tree plantation areas was comparable, 1.39 ha (SE?=?1.09) in Village A and 1.38 ha (SE?=?1.01) in Village G. These results suggest that government support is a determinant of the timing and area of tree planting activities. After the FLA, one of the next policy challenges is how to make small-scale farm forestry financially independent.  相似文献