Distribution of citrus viroids and <Emphasis Type="Italic">Apple stem grooving virus</Emphasis> on citrus trees in Japan using multiplex reverse transcription polymerase chain reaction     

Takao Ito  Nobuyuki Namba  Tsutae Ito 《Journal of General Plant Pathology》2003,69(3):205-207

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A new primer for 16S rDNA analysis of microbial communities associated with <Emphasis Type="Italic">Porphyra yezoensis</Emphasis>     

Aki Namba  Yuya Shigenobu  Masahiro Kobayashi  Takanori Kobayashi  Ichiro Oohara 《Fisheries Science》2010,76(5):873-878

To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, β-proteobacteria, γ-proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group.  相似文献   

Isolation and Characterization of Derivative Lines of the Onion Yellows Phytoplasma that Do Not Cause Stunting or Phloem Hyperplasia     

Oshima K  Shiomi T  Kuboyama T  Sawayanagi T  Nishigawa H  Kakizawa S  Miyata S  Ugaki M  Namba S 《Phytopathology》2001,91(11):1024-1029

ABSTRACT Two lines of onion yellows phytoplasma producing milder symptoms were isolated from the original line (OY-W). One has an additional characteristic, non-insect-transmissibility (OY-NIM), compared with the other (OY-M). OY-M was established after maintaining OY-W for 11 years on a plant host (Chrysanthemum coronarium) with an insect vector (Macrosteles striifrons), and OY-NIM was isolated after subsequent maintenance of OY-M in plants by periodic grafting. Polymerase chain analysis suggested that OY-NIM cannot traverse the gut or survive in the hemolymph of the leafhopper. OY-W results in witches'-broom formation and stunted growth in the host plant. In contrast, OY-M and OY-NIM do not cause stunting in the host plant, although they result in witches'-broom. Histopathological analysis of these lines revealed that the hyperplastic phloem tissue and severe phloem necrosis seen in OY-W did not exist in OY-M and OY-NIM. This was attributed to a reduction in the population of phytoplasma in tissues in both OY-M- and OY-NIM-infected plants. The results suggest that the cause of stunting and phloem hyperplasia may be genetically different from the cause of witches'-broom. Pulsed field gel electrophoresis analysis showed that OY-M had a smaller genome size ( approximately 870 kbp) than OY-W ( approximately 1,000 kbp). Thus, some of the OY-W genes responsible for pathogenicity may not be present in OY-M.  相似文献   

ECHOCARDIOGRAPHIC AND DOPPLER DIAGNOSIS: FIRST CASE OF ATRIAL SEPTAL ANEURYSM IN A CAT     

A. P. Nicolle  V. Chetboul  H. Namba  F. Crespeau  A. Tidholm  C. Carlos  V. Gouni  J. L. Pouchelon 《Veterinary radiology & ultrasound》2005,46(3):230-233

A 6-year-old female cat developed pleural and abdominal effusion. Cardiac ultrasound and 2D color tissue Doppler imaging revealed restrictive cardiomyopathy with severe biatrial dilatation and hypertension. This cardiomyopathy was associated with atrial septal aneurysm and a patent foramen ovale. The atrial septal aneurysm involved the entire atrial septum. Necropsy and histological examination confirmed all these findings. ASA is a rare malformation and, as in this cat, it generally occurs concomitantly with congenital or acquired heart disease and may be explained by greater pressure in one atrium, leading to controlateral protrusion of the atrial septum.  相似文献   

Monolayer crystallization of flagellar L-P rings by sequential addition and depletion of lipid   总被引：1，自引：0，他引：1  

T Akiba  H Yoshimura  K Namba 《Science (New York, N.Y.)》1991,252(5012):1544-1546

The L-P ring complex is thought to be a molecular bushing that supports flagellar motor rotation at about 10,000 revolutions per minute with presumably very little friction. Structural studies of this complex have been limited because only very small amount of samples are available. Therefore devising an efficient method of crystallization was essential. The addition of a phospholipid and its subsequent slow depletion by phospholipase A2 have been used to successfully grow well-ordered monolayer crystals that extend up to about 10 micrometers. The interaction of the L-P ring complex with lipid membranes was also visualized during this process.  相似文献   

Molecular epidemiology of Plum pox virus in Japan     

Maejima K  Himeno M  Komatsu K  Takinami Y  Hashimoto M  Takahashi S  Yamaji Y  Oshima K  Namba S 《Phytopathology》2011,101(5):567-574

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Quantitative single radial immunodiffusion of foot-and-mouth disease viral antigens using convalescent cattle sera     

T Tsuda  K Namba  Y Yamasaki  S Nilchavee  N Pattanaprasit  W Sinchongsubongkoie  T Onodera 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(3):461-468

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Rapid and reliable detection of phytoplasma by loop-mediated isothermal amplification targeting a housekeeping gene     

Kyoko Sugawara  Misako Himeno  Takuya Keima  Yugo Kitazawa  Kensaku Maejima  Kenro Oshima  Shigetou Namba 《Journal of General Plant Pathology》2012,78(6):389-397

Phytoplasmas are plant pathogenic bacteria that infect more than 700 plant species. Because phytoplasma-resistant cultivars are not available for the vast majority of crops, the most common practice to prevent phytoplasma diseases is to remove infected plants. Therefore, developing a rapid, accurate diagnostic method to detect a phytoplasma infection is important. Here, we developed a phytoplasma detection assay based on loop-mediated isothermal amplification (LAMP) by targeting the groEL gene and 16S rDNA. We designed 19 primer sets for the LAMP assay and evaluated their amplification efficiency, sensitivity, and spectra to select the most suitable primer sets to detect Candidatus Phytoplasma asteris. As a result, DNA was efficiently amplified by one of the primer sets targeting the groEL gene, and LAMP assay sensitivity with this primer set was 10-fold higher than that of the polymerase chain reaction. Moreover, the groEL gene was successfully amplified from several strains of Ca. Phytoplasma asteris by this primer set, indicating that the groEL gene can be used as a LAMP assay target gene for a broad range of phytoplasma strains. Additionally, a simple DNA extraction method that omits the homogenizing and phenol extraction steps was combined with the LAMP assay to develop a simple, rapid, and convenient diagnostic method for detecting phytoplasma.  相似文献   

First report of fig mosaic virus infecting common fig (Ficus carica) in Japan     

Kazuya Ishikawa  Kensaku Maejima  Susumu Nagashima  Nobuo Sawamura  Yusuke Takinami  Ken Komatsu  Masayoshi Hashimoto  Yasuyuki Yamaji  Jun Yamamoto  Shigetou Namba 《Journal of General Plant Pathology》2012,78(2):136-139

For the first time, fig mosaic virus (FMV) was detected in common fig (Ficus carica) trees in Shimane, Japan. These trees exhibited mosaic, ringspots, or distortion, accompanied by chlorosis on leaves and yellow spots on fruits. Some of the symptomatic trees were infested with the eriophyid mite Aceria ficus. The virus was detected based on RT-PCR, followed by sequencing. The amplified 300 base-pair fragments shared 83.5–91.5% identity with the corresponding region of RNA-dependent RNA polymerase gene of FMV isolates previously reported in Turkey, Iran, and Italy.  相似文献   

First report of ‘<Emphasis Type="Italic">Candidatus</Emphasis> Phytoplasma malaysianum’ associated with <Emphasis Type="Italic">Elaeocarpus</Emphasis> yellows of <Emphasis Type="Italic">Elaeocarpus zollingeri</Emphasis>     

Nozomu Iwabuchi  Ai Endo  Norikazu Kameyama  Masaya Satoh  Akio Miyazaki  Hiroaki Koinuma  Yugo Kitazawa  Kensaku Maejima  Yasuyuki Yamaji  Kenro Oshima  Shigetou Namba 《Journal of General Plant Pathology》2018,84(2):160-164

“Elaeocarpus yellows” (ELY) is a widely reported phytoplasma disease of Elaeocarpus zollingeri trees in Japan. The phytoplasma associated with ELY (ELY phytoplasma) had not been identified at the species level because its 16S rRNA sequence had yet to be reported. Here, we report the results of a sequence analysis based on 16S rRNA and secA gene sequences, which showed that the ELY phytoplasma is related to ‘Candidatus Phytoplasma malaysianum’. To our knowledge, this is the first report showing the occurrence of ‘Ca. P. malaysianum’ outside Malaysia and the infection of E. zollingeri by the phytoplasma.  相似文献