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郑州牧专牛膝课题组 《郑州牧业工程高等专科学校学报》1999,(1)
经过近1年的实验室工作,于1997年5月得到发育良好的牛膝细胞转化苗.1997年6月将室内培植14日的6棵幼苗移栽至田间.种植1O日后去弱保留3棵.1997年1O月收获种子.一部分留样,一部分于1998年5月进行田间播种,扩大种植. 相似文献
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Radix Suharjo Hiroyuki Sawada Yuichi Takikawa 《Journal of General Plant Pathology》2014,80(3):237-254
Forty-one representative Japanese Dickeya spp. (Erwinia chrysanthemi) strains isolated from 24 plants in Japan were investigated using multilocus sequence analysis of recA, dnaX, rpoD, gyrB and 16S rDNA; PCR–RFLP (restriction fragment length polymorphism) of recA, rpoD and gyrB genes; PCR genomic fingerprinting; and biochemical tests. Based on the recA, dnaX, rpoD, gyrB and 16S rDNA sequences and PCR genomic fingerprinting, the strains were essentially divided into six groups (I–VI). Group I corresponded to D. chrysanthemi, group II corresponded to D. dadantii, group III to D. dianthicola and group IV to D. zeae. Meanwhile, group V and group VI could not be assigned to any existing Dickeya species, and they were deduced to be two putative new species. The PCR–RFLP analysis of gyrB, rpoD and recA clearly differentiated the six groups of Dickeya strains. From the results of the biochemical tests, the strains were assigned to biovars 1, 3, 5, 8 and 9; only one strain (SUPP 2525) was not assignable to the existing biovars. We also showed that the PCR–RFLP analysis of rpoD, gyrB and recA can be used as a rapid technique to identify Japanese Dickeya strains. 相似文献
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Walnut is one of the major regional crops produced in Dauphiné (SE France). Unfortunately, walnut blight has been recorded as the main disease for years. Crop losses due to this disease may reach high levels in spite of repeated copper sprays. In order to monitor the bacterial contamination of the fruits, two orchards differing in soil, irrigation and treatment were selected. Fruit sampling was performed once a week for 11 weeks. Results showed that the percentage of contaminated fruits was more important at the beginning of a maturation, that bacterial populations rapidly reached a maximum level and that there was no obvious relation between the number of bacteria infecting a fruit and the occurrence of necrosis. These results did not indicate any significant difference between the two orchards, in these respects. 相似文献
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