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In this study, the effect of various vermicompost substitutions (0, 25, 50, and 75%) was investigated on germination, growth and development, photosynthetic parameters, the essential oil content, and disease resistance of Thymus vulgaris. Results showed that 25% vermicompost substitution promoted the best seedling emergence indices, whereas maximum length, fresh and dry weight of aerial parts and root, chlorophyll and carotenoid contents, photosynthetic efficiency, and the highest essential oil content were observed in 50% vermicompost substitution. Vermicompost was an effective biocontrol agent against Fusarium oxysporum and Phytophthora infestans. Suppression of pathogens by vermicompost was enhanced progressively with increasing rates of substitution of vermicompost, and the highest suppression obtained in 75% vermicompost substitution. The disease protection correlated to development of activities of defense-related enzymes including β-1,3-glucanase, phenylalanine ammonialyase, polyphenol oxidase and peroxidase, and total phenolics content. These results suggest the potential of vermicompost in promoting of plant yield as well as inducing systemic resistance in Thymus vulgaris.  相似文献   
2.
In DAS-ELISAs of 86 grapevine samples from northwestern Iran, Grapevine fanleaf virus (GFLV) was detected in 18 samples. RT-PCR with two primer pairs (M2/M4 or M0/M4) corresponding to GFLV movement protein (MP) amplified the expected 854- and/or 1,489-bp fragment(s), respectively, from all ELISA-positive samples. Four smaller and three larger PCR products were cloned and sequenced, which revealed that the MP region of the isolates was 1,044 nucleotides (nt) long, corresponding to the GFLV MP. There were 83–86% nucleotide and 93–94% amino acid identities deduced between the MPs of the sequenced isolates. Nucleotide sequence identities of 81–87 and 75–79% were found between the MP regions of these isolates and that of previously published GFLV and Arabis mosaic virus (ArMV) strains/isolates, respectively. On a consensus parsimony tree based on the nucleotide sequences, isolates La208 and X300 remained distinct from previously reported GFLVs. This is the first molecular characterization of GFLV MP isolates from Iran. The sequence data reported in this paper have been submitted to the DDBJ/EMBL/GenBank databases and have been assigned accession numbers DQ286901 to DQ286916.  相似文献   
3.
Maedi-visna (MV) is an important slow viral disease of sheep leading to a progressive lymphoproliferative disease. It affects multiple organs primarily the lungs, where it causes interstitial pneumonia (maedi). In this study, the lungs of 1,000 sheep carcasses were grossly inspected and those suspected to have maedi were studied at histopathological and molecular levels. A polymerase chain reaction (PCR) technique that amplified a 291-base pair DNA in the long terminal repeat (LTR) sequence of MV provirus was conducted on all the 50 suspected lungs together with 10 normal appearing lungs as controls. Amplicons of the expected size were detected in 11 (n = 11/50) suspected sheep, and one of the 10 control sheep. Histopathologic study of the pulmonary lesions of all 11 (n = 11/11) positive sheep showed MV lesions, including hyperplasia of the perivascular and peribronchiolar lymphoid cells, interstitial lymphoplasmacytic infiltration and smooth muscle hyperplasia and the histopathologic findings were correlated with PCR results. In contrast, the tissue sections of control animals were almost normal at histopathological level; however, PCR technique demonstrated that one of them was affected by maedi. This study showed that the LTR-PCR had high specificity and sensitivity in diagnosis of this viral infection. This study is the first to evaluate the prevalence of MV virus infection in sheep in Iran.  相似文献   
4.
Eurasian Soil Science - The major goal of this research was to assess the ability of visible-near-infrared (VIS-NIR) spectroscopy combined with multivariate regressions based on four modeling...  相似文献   
5.
The physiological basis of genetic variation in drought response and its association with yield and related indices is not clear in tall fescue. In this study thirty genotypes of tall fescue (Festuca arundinacea Schreb.) were sampled from a polycross population and evaluated under two levels of irrigation in 2010 (normal and intense stress) and 2011 (normal and mild stress). Physiological traits including relative water content (RWC), total chlorophyll (TChl), chlorophyll a (Chla), chlorophyll b (Chlb), Chla/Chlb, carotenoids (Car), TChl/Car and proline content along with forage yield, agro-morpholgical traits and selection indices (stress tolerance index, STI and drought susceptibility index, DSI) were studied. Large variation and moderate to high heritability was estimated for most of the studied traits. Intense drought condition decreased chlorophyll content while mild stress significantly increased it. In the other hand intense drought stress increased Chla/b while mild stress didn’t change it. Under mild drought stress condition STI was positively correlated with RWC while under intense drought stress condition STI was positively correlated with chlorophyll content. Although proline content was significantly increased in both intense and mild drought stress conditions, no relationship was found between proline accumulation with forage yield and STI. Applications of principle component analysis for screening suitable genotypes are also discussed.  相似文献   
6.
In this paper, the surface of magnetic manganese ferrite nanoparticles (MFN) was modified using cetyl trimethylammonium bromide (CTAB). The modified MFN was studied using Fourier transform infrared spectroscopy (FTIR). The adsorption capacity of surface modified MFN (MFN-CTAB) was investigated for dye removal for single and ternary systems. Three anionic dyes, C.I. Direct Red 80 (DR80), C.I. Direct Red 31 (DR31), and C.I. Acid Blue 92 (AB92), were used as model compounds. The effects of operational parameters on dye removal (i.e. adsorbent dosage, dye concentration and salt) and the kinetic and isotherm of dye adsorption were studied. The adsorption kinetic for the dyes was found to be well described by the pseudo-second order model. The maximum dye adsorption capacity (Q 0) of MFN-CTAB for DR80, DR31 and AB92 was 83 mg/g, 59 mg/g and 70 mg/g, respectively. The adsorption isotherm data were analyzed using the Langmuir, Freundlich, and Temkin equations. The results revealed that the Langmuir model fitted the adsorption data better. The results showed that the MFN-CTAB as a magnetic adsorbent might be a suitable alternative to remove dyes from colored aqueous solutions.  相似文献   
7.
Using pedotransfer functions (PTF) is a useful way for field capacity (FC) and permanent wilting point (PWP) prediction. The aim of this study was to model PTF to estimate FC and PWP using regression tree (RT) and stepwise multiple linear regressions (SMLR). For this purpose, 165 and 45 soil samples from UNSODA and HYPRES datasets were used for development and validation of new PTFs, respectively. %Clay, geometric mean diameter (dg), and bulk density (BD) were selected as predictor variables due to the highest correlation and lowest multicollinearity. The results showed that clay percentage with W* = 0.89 and dg with W* = ?0.57 were the most effective variables to predict PWP and FC, respectively. The RT method had a better performance (R2 = 0.80, ME = ?0.002 cm3cm?3, RMSE = 0.05 cm3cm?3 for FC and R2 = 0.85, ME = 0.003 cm3cm?3, RMSE = 0.03 cm3 cm?3 for PWP) than SMLR in estimation of FC and PWP.  相似文献   
8.
Temperature is a key factor for the living organisms on earth. It influences weed management practices, either directly or indirectly. Field experiments were conducted to determine the effect of temperature on the postdispersal seed predation of four important weed species (Cuscuta compestris, Stellaria media, Taraxacum officinale and Veronica persica) in two lucerne fields in Mashhad and Chenaran, north‐eastern Iran. These two cities have the same climate: temperate and cold alpine but the temperature varies between them. Wire mesh cages were used to determine the relative importance of birds in predation and pitfall traps were used to detect the species and the activity density of invertebrate predators. The results showed that the predation preference of different weed species was significantly different between and within fields. Seed predation fluctuated widely throughout the sampling periods, matching the periodic forage harvest and regrowth cycle of lucerne. Despite the level of seed predation fluctuating, it declined toward the last sampling periods. Using wire mesh exclusion cages showed no significant effect of birds on weed seed predation in both fields. Ants, crickets and carabid beetles were the invertebrate seed predators that were caught in the pitfall traps. There were significant correlations between the mean temperature and predator activity densities and also between the predated seeds and the mean air temperature in both locations. The results of this study indicate the significant effect of temperature on postdispersal weed seed predation. Therefore, with respect to climate change and increasing global warming, it would be possible to focus on postdispersal seed predation in weed management in the future.  相似文献   
9.
The occurrence of ovarian tumors is uncommon in domestic animals. Reports are documented more often in the bitch, mare, and cow. In the mare, granulosa-thecal cell tumor is the most common ovarian neoplasms, and other tumors occur less often. Fibrothecoma is a rare gonadal-stromal tumor of the ovary. There is only one reported case in the veterinary literature. This tumor consists of theca cells and fibrous tissues. The present study describes a rare case of fibrothecoma in a 10-year-old Arabian mare that was presented due to infertility problems. The abnormal left ovary was detected via rectal palpation and ultrasonography. Postoperation study showed a solid tumoral mass that completely replaced the normal ovarian tissues. The tumor was large, firm in consistency, with smooth surface. On the cut surface, the mass was white-gray to yellow with cystic areas containing green gelatinous materials. Microscopic examination revealed the structure was composed of fibroblastic cell producing collagen fibers and theca cells containing lipids. Based on histopathologic features, diagnosis of fibrothecoma was confirmed postoperatively.  相似文献   
10.
Serum lipid abnormalities are an established risk factor for cardiovascular disease in hyperlipidemic patients. Because of the side effect of long-term medications in these patients, soy protein, as a part of healthy diet, may improve their lipid profile. The purpose of this study was to determine the effect of soy protein with low-calorie diet on lipid profiles in hyperlipidemic patient. Fifty two hyperlipidemic patients of both sex and age range of 25-65 years were participated. The patients received low-calorie diet based on 1400 kcal energy 18% protein, 24% fat and 58% carbohydrate per day for 4 weeks. The treatment group received low-calorie diet including (30 g day(-1)) of soy protein. Blood samples were obtained after an overnight (12 h) fasting period before the study and in the last day of the intervention period. Anthropometric indices and levels of serum triacylglycerol, total cholesterol, low-density lipoprotein and high-density lipoprotein were measured before and after the intervention. In groups, weight, body mass index, waist and hip circumferences were significantly reduced (p<0.05) after four weeks. In comparison with control group, the results of treatment of soy protein with low-calorie diet showed that at the end of 4th week serum low-density lipoprotein was significantly (p<0.05) decreased while an insignificant reduction was seen in total cholesterol. This study suggest that soy protein with low calorie diet for 4 weeks treatment may be safe and effective alternative therapy for diminished the cardiovascular risk factors in hyperlipidemic patients.  相似文献   
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