Protein synthesis measured in the whole body of chick embryos cultured in vitro     

K. Kita  S. Nakagawa  R. Pinontoan  J. Okumura  T. Muramatsu 《British poultry science》1993,34(5):1043-1047

1. Whole body protein synthesis was measured in chick embryos cultured in vitro. On day 7 of incubation chick embryos were cultured for 60 min in synthetic serum‐free medium containing 4‐[³H]phenylalanine. Specific radioactivities in free and protein‐bound phenylaline in the whole embryo were measured, starting 2 min after commencement of the culture process.

2. The values for fractional synthesis rate (FSR) estimated in vitro at 20, 30, 45 and 60 min during the embryo culture agreed well, ranging from 35 to 40%/d, suggesting that the method would serve as a useful model for studying the effect of growth promoters in chick embryos.

3. Bovine insulin in the synthetic medium did not affect FSR of protein in chick embryos cultured in vitro.  相似文献   

The rescue of Epstein--Barr virus from primary cultured cells of nasopharyngeal carcinoma.     

M Takada  M Murata  M Takei  S Nakagawa  H Okumura  A Kawamura 《Comparative immunology, microbiology and infectious diseases》1979,2(2-3):177-189

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Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface layer of flooded paddy field soil     

Nakagawa  Koki  Murase  Jun  Asakawa  Susumu  Watanabe  Takeshi 《Journal of Soils and Sediments》2020,20(11):4034-4041

Journal of Soils and Sediments - To reveal whether microaerophilic Fe(II)-oxidizing bacteria (FeOB) participate in the Fe(II) oxidation at the oxic-anoxic interface in flooded paddy field soil,...  相似文献   

Identification of the core rumen bacterial taxa and their population dynamics during the fattening period in Japanese Black cattle     

Hiroto Miura  Takuya Hashimoto  Yukiko Kawanishi  Hiroki Kawauchi  Ryo Inoue  Noriaki Shoji  Kunihiko Saito  Mario Sekiya  Yosuke Saito  Jumpei Yasuda  Chiemi Yonezawa  Tetsushiro Endo  Hirotaka Kasuya  Yutaka Suzuki  Yasuo Kobayashi  Satoshi Koike 《Animal Science Journal》2021,92(1):e13601

The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period.  相似文献   

Preventive effects of CIDR-based protocols on premature ovulation before timed-AI in Ovsynch in cycling beef cows     

Sakase M  Kawate N  Nakagawa C  Fukushima M  Noda M  Takeda K  Ueno S  Inaba T  Kida K  Tamada H  Sawada T 《Veterinary journal (London, England : 1997)》2007,173(3):691-693

Ovsynch is a program developed to synchronize ovulation for timed breeding. In this paper, the authors investigate whether controlled internal drug release (CIDR)-based protocols prevent premature ovulation before timed-artificial insemination (AI) when Ovsynch is started a few days before luteolysis in cycling beef cows. Nine beef cows at 16 days after oestrus were treated with (1) Ovsynch, i.e. gonadotropin releasing hormone (GnRH) analogue on day 0, prostaglandin (PG) F(2alpha) analogue on day 7 and GnRH analogue on day 9 with timed-AI on day 10, (n=3); (2) Ovsynch+CIDR (Ovsynch protocol plus a CIDR for 7 days from day 0, n=3), or (3) oestradiol benzoate (OB)+CIDR+GnRH (OB on day 0 in lieu of the first GnRH treatment, followed by the Ovsynch+CIDR protocol, n=3). In the Ovsynch group (1) plasma progesterone concentrations fell below 0.5 ng/mL earlier (day 5) than in both CIDR-treated groups (2) and (3), where this occurred on day 8. Plasma oestradiol-17beta concentrations peaked on day 8 in the Ovsynch group and on day 9 in both CIDR-treated groups. The dominant follicle ovulated on day 10 in the Ovsynch group and on day 11 in both CIDR-treated groups. Thus, both CIDR-based protocols prevented premature ovulation before timed-AI in Ovsynch when the protocol was started a few days before luteolysis. This reflects the fact that progesterone levels remained high until the beef cattle were treated with PGF(2alpha).  相似文献   

Quantitative structure-activity analysis of larvicidal 1-(substituted benzoyl)-2-benzoyl-1-tert-butylhydrazines against Chilo suppressalis     

Nobuhiro Oikawa  Yoshiaki Nakagawa  Keiichiro Nishimura  Tamio Ueno  Toshio Fujita 《Pest management science》1994,41(2):139-147

The larvicidal activity of a number of 1-(substituted benzoyl)-2-benzoyl–1 -ten-butylhydrazines against the rice stem borer (Chilo suppressalis Walk.) was measured. Variations in the activity were examined quantitatively using physico-chemical substituent and molecular parameters and regression analysis. The results indicated that the molecular hydrophobicity and the electron-withdrawing inductive/ field effect of ontho substituents are favourable to larvicidal activity. The bulkiness of substituents at the meta and para positions was unfavourable to activity, substitution at the para position being more unfavourable than that at the meta position in terms of van der Waals' volume. The 2,3–, 2,5- and 2,6-disubstitution patterns were also unfavourable to activity. Reductions in larvicidal activity caused by the 2,6-,- 2,3,5- and 2,3,4,5-substitutions were greater than those induced by the 2,3- and 2,5-disubstitutions. When the sum of contributions from favourable effects is greater than that from unfavourable effects, the larvicidal activity is expected to be superior to that of the unsubstituted compound.  相似文献   

Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha     

Shoji Y  Inoue Y  Sugisawa H  Itou T  Endo T  Sakai T 《Veterinary immunology and immunopathology》2001,82(3-4):183-192

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Application of short-time magnetic resonance examination for intervertebral disc diseases in dogs     

Yamada K  Nakagawa M  Kato T  Shigeno S  Hirose T  Miyahara K  Sato M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(1):51-54

The usefulness of magnetic resonance (MR) is already established, but it has a disadvantage of requiring a long scanning time. A short-time examination is more or less needed so as to be more practical in veterinary clinics. A protocol of the short-time MR examination was devised based on parameters determined, and validity of the protocol was assessed through the diagnosis of clinical cases with intervertebral disc diseases. With this protocol, it was possible to complete an MR examination for the spine within 15 min. The MR images and myelographic findings were correlated well in this study, suggesting the short-time protocol of MR examination can be used in the clinical diagnosis of spinal diseases.  相似文献   

Proportions of melanocyte stimulating hormone-immunoreactive cells in the adenohypophysis of Silky fowl and hyperpigmentation-free cockerels     

Shotaro NISHIMURA  Hitomi SHIMODA  Ichiro OSHIMA  Yoshitaka ONO  Kaoru OKANO  Akira ISHIBASHI  Shoji TABATA  Hisao IWAMOTO 《Animal Science Journal》2005,76(6):575-579

Alpha‐melanocyte stimulating hormone (α‐MSH) is one of the main regulators for melanocytes, and the adenohypophysis is one of the major tissues for the synthesis of this hormone. The Silky fowl is a characteristic breed of chicken with hyperpigmentation throughout the body. The involvement of the adenohypophysis in the hyperpigmentation of this breed is not known. In the present study, the proportion of melanocyte stimulating hormone‐immunopositive cells (MSH cells) in the adenohypophysis was immunocytochemically compared between Silky, Red Cornish × New Hampshire (RN) crossbred and Japanese bantam cockerels at 15 weeks of age. After the body and gland were weighed, the adenohypophyseal cells were enzymatically dispersed and immunostained for α‐MSH, and the immunopositive MSH cells were counted. The weights of the body and adenohypophysis were heaviest in the RN crossbred, followed by the Silky and lightest in the bantam cockerels. In contrast, the ratio between adenohypophysis and bodyweight was much larger in the Silky and the bantam than in the RN crossbreed (P < 0.05). The population of MSH cells in the adenohypophysis was larger in the Silky (14.3%) than in the RN crossbreed (8.0%) and the bantam (8.1%) cockerels (P < 0.05). From these results, it was concluded that prepubertal Silky cockerel have numerous MSH cells in the adenohypophysis suggesting a relationship to hyperpigmentation.  相似文献   

Quantitative structure—activity relationships of light-dependent herbicidal 4-pyridone-3-carboxanilide derivatives II. Substituent effects of anilide and pyridone moieties     

Hirokazu Osabe  Yasuo Morishima  Yukihisa Goto  Kazuhisa Masamoto  Yoshiaki Nakagawa  Toshio Fujita 《Pest management science》1992,34(1):27-36

The variations in the light-dependent herbicidal activity against Echinochloa oryzicola of a number of 4-pyridone-3-carboxanilides with various substituents on the anilide and pyridone rings have been examined by use of physicochemical substituent parameters and regression analysis. The effects of substituents of the anilide ring were such that the activity was related parabolically not only with the substituent hydrophobicity (π) but also with the steric bulk parameter (E_s) of the bulkier ortho substituent. The effect of substituents at the 1-position of the pyridone ring was analysed with steric(STERIMOL L and B₅) and electronic(γ₁) parameters. That of substituents at the 5-position of the pyridone ring was explained by a parabolic function of a steric (MR) parameter. Analyses of the activity of 105 analogs confirmed that our previous selection of a candidate compound in this series for field trials was indeed appropriate in terms of physicochemical substituent effects.  相似文献