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Daniela Moyankova Aneta Lyubenova Slavtcho Slavov Dimitar Djilianov 《European journal of plant pathology / European Foundation for Plant Pathology》2014,138(1):149-155
The Phytophthora pathogens are among the most important pests in the modern agriculture and forestry. This makes them a subject of intensive studies, including laboratory experiments with in vitro culture on various plant-derived media. Here we show that leaf extracts from the resurrection endemic plant Haberlea rhodopensis stimulates significantly the in vitro growth of various isolates from several Phytophthora species. The extracts stimulate the formation of oospores in some heterothallic species. In this respect, the idea to propose Haberlea extracts as a potential ingredient of culture media for Phytophthora spp. maintenance under controlled conditions or as a component of detection systems for Phytophthora presence in nature seems quite attractive and promising. 相似文献
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Petya Koeva Christova Aneta B. Lyubenova Kaloyan V. Kostov Slavtcho B. Slavov 《Forest Pathology》2019,49(3)
Two Phytophthora pseudosyringae isolates were recovered from aquatic ecosystems in Bulgaria during a two‐year investigation of Phytophthora distribution in water sources in the country. Isolate RVit2016/6d was derived from Boyana Lake at the Vitosha Mountain, whereas isolate RTr2016/32d was obtained from Erma River at the Ruy Mountain. Both isolates belong to P. pseudosyringae species according to their morphological and physiological characteristics, as well as to the DNA sequence analysis of the internal transcribed spacer (ITS) region. The pathogenicity of the isolates to wild cranberry plants (Vaccinium vitis‐idea) was studied by detached leaves experiments and in planta. Both P. pseudosyringae isolates were able to cause leaf necrosis and death of plants within 3 months. The ability of the pathogens to infect cranberry leaves at different temperatures was also investigated. The significance of P. pseudosyringae species and its potential threat for forest ecosystems is discussed. To our knowledge, this is the first report of P. pseudosyringae isolation in Bulgaria. 相似文献
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In vitro culture of Orobanche ramosa 总被引:3,自引:0,他引:3
Orobanche spp. (broomrapes) are holoparasites that subsist on the roots of many important crops and can considerably reduce yield. The control of Orobanche spp. includes physical, chemical and biological methods. Interactions between parasitic angiosperms and their hosts first occur at the level of parasite seed germination. The seeds of all Orobanchaceae germinate in soil under natural conditions only in response to specific chemical exudates from the host plant. This study describes the influence of different plant growth regulators and host plant root exudates on germination and development of calli from Orobanche seeds in vitro . The effect of indole-3-acetic acid, gibberellic acid and kinetin on the germination of Orobanche seeds varied with concentration. These plant growth regulators also affected the period of germination and the structure of calli and protrusions. An in vitro system for the collection of tobacco root exudates was established. Compounds released from the host roots of three different tobacco cultivars were found to provoke high levels of germination of the Orobanche seeds without any period of pre-conditioning. This study developed methods for the investigation of host–parasite interactions and the effect of germination stimulants in Orobanche spp. 相似文献
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Christova Petya K. Dincheva Ivaiyla N. Slavov Slavtcho B. Dobreva Ana M. Yordanova Zhenia P. Dimkova Stela D. Zaprianova Nadejda G. Atanassova Bistra Y. Sobiczewski Piotr Iakimova Elena T. 《植物病害和植物保护杂志》2021,128(6):1531-1545
Journal of Plant Diseases and Protection - The growth response of three phytopathogens to inhibitory activity of essential oils (EOs), derived from three monarda (Monarda didyma L.) genotypes, was... 相似文献
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Evgeni Slavov Ivan Penchev Georgiev Petko Dzhelebov Ivan Kanelov Maria Andonova Teodora Mircheva Georgieva Silviya Dimitrova 《Veterinary research communications》2010,34(3):205-215
As obesity is a state of low-grade inflammation, we aimed to investigate the combined effect of high-fat diet and bacterial
infection on β-cell function and insulin sensitivity in dogs. We used 20 healthy, male, mongrel dogs randomly divided into
four groups: control group—healthy, non-obese dogs; infected group—non-obese dogs with experimentally induced infection (Staphylococcus intermedius); obese group—obese dogs (after 90 day high-fat diet) and obese-infected group—obese dogs with experimentally induced infection
(Staphylococcus intermedius). To evaluate insulin sensitivity and β-cell function an intravenous glucose tolerance test (IVGTT) was performed. Plasma
insulin increased in all group after glucose infusion. The lowest values were found in obese-infected group. Blood glucose
also increased on 3 min after glucose infusion and then gradually decreased. In obese-infected group glucose concentration
on 30 min was still significantly higher than initial levels, while in other groups glucose concentration returned to the
initial values. The lowest rate of glucose elimination was found in infected group. In dogs of obese group and obese-infected
group AUCins 0–60 min was lower compared to controls. AUCglucose 0–60 min values were lowest in infected group, while in obese-infectd group values were the highest. Levels of ∆I/∆G in dogs of obese-infected
group were significantly lower compared to controls and infected group. In conclusion, these results reveal that infection
in obese dogs leads to impaired glucose tolerance, which is result of impairment in both insulin secretion and insulin sensitivity. 相似文献
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Simeonova GP Slavov E Usunov R Halacheva K Dinev DN 《Veterinary research communications》2008,32(8):619-626
The objective of the study was to investigate the hypothesis that perioperative lymphocytopenia was due to apoptosis of these
cells induced by either halothane or epidural anaesthesia in dogs. The relationship between apoptosis induction and plasma
concentrations of the stress hormone cortisol and the cytokines TNF-alpha and IL-10 was examined as well. The study was performed
on 22 healthy mongrel dogs, equal numbers from both genders, weighing 18.3 ± 2.9 kg, and aged between 3–5 years. Dogs were
divided in three groups. Eight of the animals were anaesthetized with halothane, another eight received epidural anaesthesia
using lidocaine, and six served as controls. Venous blood samples were obtained immediately before (0 minute) anaesthesia,
during deep anaesthesia (120 minute), and on the next day (24 hour) in order to determine the following parameters: the total
lymphocyte counts, the percentage of apoptotic peripheral blood mononuclear cells (PBMC) by flow cytometry, plasma concentrations
of the cytokines tumor necrosis factor – alpha (TNF-alpha) and interleukin – 10 (IL-10) by enzyme-linked immunosorbent assay
(ЕLISA), and plasma cortisol levels by radioimmune assay. Both halothane and epidural anaesthesia in dogs induces apoptosis
of PBMC with slight decrease in total lymphocyte counts. These immunomodulatory effects were transient and faded till the
24th hour. Concerning the mechanism of inducing lymphocyte apoptosis by general or epidural anaesthesia, it seemed that neither
cortisol, nor the tested cytokines TNF-alpha and IL-10 were implicated in this process. Further investigations are necessary
to confirm this assumption. 相似文献
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