Assessing seed desiccation responses of native trees in the Caribbean     

Mattana  Efisio  Peguero  Brígido  Di Sacco  Alice  Agramonte  Wilvin  Encarnación Castillo  Wilkin Rafael  Jiménez  Francisco  Clase  Teodoro  Pritchard  Hugh W.  Gómez-Barreiro  Pablo  Castillo-Lorenzo  Elena  Terrero Encarnación  Marianny  Way  Michael J.  García  Ricardo  Ulian  Tiziana 《New Forests》2020,51(4):705-721

New Forests - Native trees from the Caribbean were tested for seed desiccation responses, by adapting the “100-seed test” protocol. Ninety-seven seed lots of 91 species were collected...  相似文献   

B-mode ultrasound examination of canine mammary gland neoplastic lesions of small size (diameter < 2 cm)     

Iacopo Vannozzi  Matteo Tesi  Marta Zangheri  Viola Maria Innocenti  Alessandra Rota  Simonetta Citi  Alessandro Poli 《Veterinary research communications》2018,42(2):137-143

Ultrasonography is a valuable tool for the evaluation of neoplastic lesions in the dog and there is a growing interest in the use of this technique for the stadiation of canine mammary tumours. An accurate assessment of small sized nodules facilitates the stadiation of the mammary lesions and helps the clinician in the choice of the most indicated surgical therapy. The aim of this study was to identify those ultrasound criteria that may be useful in discriminating between benign and malignant lesions of small size (diameter smaller than 2 cm). Sixty-two nodules, < 2 cm in larger diameter, belonging to thirty-five bitches presented between January 2012 and February 2014 were evaluated. Tumours were observed by conventional ultrasound and assessed for: shape (regular-irregular), limit (defined-ill-defined), margins (regular-irregular), echogenicity (hypoechoic-isoechoic-hyperecoic), echotexture (homogeneus-heterogeneus), presence of hyperecoic halo, distal acoustic enhancement or shadowing and surrounding tissue alterations. Among the alterations in surrounding tissues, the disruption of the glandular tissue and the increase in echogenicity of the peritumoral tissues were assessed. Thereafter, bitches were subjected to mastectomy and nodules were evaluated histologically. None of the ultasound criteria considered in the current study showed a statistically significant relation with malignancy, except for the presence of alterations in the tissue surrounding the nodules. According to our results, this characteristic may indicate malignancy, however its subjectivity may affect the applicability in clinical practice. In conclusions, conventional ultrasound in bitches had a limited ability in discriminating benign and malignant mammary gland neoplastic lesions of small size (diameter?<?2 cm).  相似文献   

Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology     

Chiara Gomiero  Giulia Bertolutti  Tiziana Martinello  Nathalie Van Bruaene  Sarah Y. Broeckx  Marco Patruno  Jan H. Spaas 《Veterinary research communications》2016,40(1):39-48

Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.  相似文献   

Comparison of six microscopic methods and two operators for estimation of white and red blood cells in canine urine sediment     

Marco Giraldi  Filippo Tagliasacchi  Saverio Paltrinieri  Tiziana Vitiello  Paola Rossetti  Paola Scarpa 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(1):71-78

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Synthesis and in-vitro antifungal activity of 6-trifluoromethyipyrazolo[3,4-6d]pyrimidin-4(5H)-thiones     

Chiara B. Vicentini  Tiziana Poli  Augusto C. Veronese  Vincenzo Brandolini  Maurizio Manfrini  Mario Guarneri  Paolo Giori 《Pest management science》1989,27(1):77-83

Twelve 6-trifluoromethylpyrazolo[3,4-d]pyrimidin-4(5H)-thiones were prepared by the reaction between 4-thiocarbamoyl-5-aminopyrazoles and trifluoroacetic anhydride. They were tested in vitro for antifungal activity against a series of phytopathogenic fungi of different taxonomic classes. The EC50 and MIC values of four compounds were comparable or inferior to those of reference commercial fungicides in controlling Sclerotinia minor, Corticium solani and Phytium ultimum.  相似文献   

Fungitoxicity of 5-aminoisoxazole-4-thiocyanate derivatives     

Chiara B. Vicentini  Vincenzo Brandolini  Tiziana Poli  Mario Guarneri  Paolo Giori 《Pest management science》1992,34(2):127-131

3-Methyl-5-(acyl/alkyl)aminoisoxazole-4-thiocyanates were prepared by rhodanation of N-acyl/alkyl-3-methyl-5-isoxazolamines. These products were tested for antifungal activity against a series of phytopathogenic fungi of different taxonomic classes. Some of the compounds showed interesting in-vitro activity. The more active compound in the in-vitro test displayed a moderate preventive activity against Plasmopara viticola, Alternaria solani and Pyricularia recondita.  相似文献   

Feline leishmaniosis due to Leishmania infantum in Italy     

Poli A  Abramo F  Barsotti P  Leva S  Gramiccia M  Ludovisi A  Mancianti F 《Veterinary parasitology》2002,106(3):181-191

A case of leishmaniosis in domestic cats (Felis catus domesticus) is described. The subject showed a nodular lesion on the eyelid. The diagnosis was achieved by serological, parasitological, and light and electron microscopic investigations. By molecular techniques the aetiological agent was identified as belonging to Leishmania infantum, the species implicated in human and canine leishmaniosis in southern Europe. A preliminary study on the prevalence of asymptomatic feline leishmaniosis, performed in the areas where the infected cat was identified, revealed a low seroprevalence of infection: only 1 (0.9%) of the 110 cat sera examined by indirect fluorescent antibody test was positive for anti-Leishmania antibodies. Because clinical signs in feline leishmaniosis are unspecific and similar to those observed in other diseases commonly found in this species, leishmaniosis must be added to the differential diagnosis by feline veterinary practitioners and adequate serologic and histopathologic investigations must be performed in endemic areas.  相似文献   

Immunopathology of the eye: Purification of canine retinal “S” antigen     

C. Secchi  L. Oldani  C. Peruccio  M. Guttinger  A. Berrini  C. Caldora  G. Poli 《Comparative immunology, microbiology and infectious diseases》1985,8(3-4):297-303

Canine retinal S antigen has been purified to study the retinal progressive atrophy of the dog. The purified antigen will be used to detect, by the ELISA technique, specific autoantibody in dogs with ocular diseases.  相似文献   

Analysis of pharmaceutically relevant taxoids in wild yew trees from Sardinia     

Ballero M  Loi MC  van Rozendaal EL  van Beek TA  van de Haar C  Poli F  Appendino G 《Fitoterapia》2003,74(1-2):34-39

Needles from a series of wild yews (Taxus baccata L.) from Sardinia were investigated for their contents of 10-deacetyl baccatin III (DAB-III, 1), paclitaxel (Taxol) (2) and taxine (3). Despite a common geographical origin, ample variation of the taxoid profile was discovered, and several samples were surprisingly devoid of all terpenoid markers above. This finding is unprecedented within the European yew, while the general lack of taxine might rationalize the observation that most plants investigated are actively and impunently browsed by goats.  相似文献   

Comparison of FEDESI and SCORFAD scoring systems for the evaluation of skin lesions in allergic cats          下载免费PDF全文

Chiara Noli  Tiziana Cena 《Veterinary dermatology》2015,26(6):481-e113

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