Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves     

Brajesh C. Varshney  N.M. Ponnanna  Pranati A. Sarkar  Pragna Rehman  Jigar H. Shah 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):57-64

Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to purified K99 antigen were produced by the hybridoma technique, and a specific clone, NEK99-5.6.12, was selected for propagation in tissue culture. The antibodies, thus obtained, were affinity-purified, characterized and coated onto Giemsa-stained Cowan-I strain of Staphylococcus aureus (S. aureus). The antibody-coated S. aureus were used in a co-agglutination test to detect K99+ E. coli isolated from feces of diarrheic calves. The specificity of the test was validated against reference monoclonal antibodies used in co-agglutination tests, as well as in ELISA. Specificity of the monoclonal antibodies was also tested against various Gram negative bacteria. The developed antibodies specifically detected purified K99 antigen in immunoblots, as well as K99+ E. coli in ELISA and co-agglutination tests. The co-agglutination test was specific and convenient for large-scale screening of K99+ E. coli isolates.  相似文献   

Development of a new CMS system in pigeonpea utilizing crosses with Cajanus lanceolatus (WV Fitgz) van der Maesen     

Sandhya Srikanth  Rachit K. Saxena  M. V. Rao  Rajeev K. Varshney  Nalini Mallikarjuna 《Euphytica》2015,204(2):289-302

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Identification of a non-redundant set of 202 in silico SSR markers and applicability of a select set in chickpea (Cicer arietinum L.)     

Gaurav Agarwal  Murali M. Sabbavarapu  Vikas K. Singh  Mahendar Thudi  S. Sheelamary  Pooran M. Gaur  Rajeev K. Varshney 《Euphytica》2015,205(2):381-394

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Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard   总被引：1，自引：0，他引：1  

A. K. Mukherjee    T. Mohapatra    A. Varshney    R. Sharma  R. P. Sharma   《Plant Breeding》2001,120(6):483-497

Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC‐144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC‐144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0_l000 and OPB06₁₀₀₀, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes.  相似文献   

Role of Excretory–Secretory Metabolites of Fasciola gigantica in Modulating Delayed-type Hypersensitivity in Rats     

Ganga G  Varshney JP  Sharma RL 《Veterinary research communications》2004,28(5):387-393

The role of excretory-secretory metabolites of Fasciola gigantica in modulating the delayed type of hypersensitivity in the host (rats) was investigated. Eighteen rats of either sex, aged 3-4 months, were assigned to three groups of 6 animals each. Rats in group 1 served as non-inoculated controls and each rat in this group was administered only Freund's complete adjuvant on day 7. Animals in groups 2 and 3 were administered inoculation dose(s) of somatic F gigantica antigen (SFgA) and excretory-secretory F gigantica antigens (ESFgA) according to the experimental schedule. The delayed-type hypersensitivity was monitored by assessing alterations in the foot pad thickness, its histopathology and lymphocyte proliferation assay. It was observed that the ESFgA caused diminution in delayed-type hypersensitivity response to a significant level (p <0.01) against SFgA in rats. This finding was further confirmed by lower stimulation indices of peripheral blood mononuclear cell in rats sensitized with ESFgA prior to inoculation of SFgA (group 1) than in nonsensitized rats receiving only SFgA (group 2).  相似文献   

Low level of polymorphism detected by SSR probes in bread wheat   总被引：2，自引：1，他引：1  

R. K. Varshney    P. C. Sharma    P. K. Gupta    H. S. Balyan    B. Ramesh    J. K. Roy    A. Kumar  A. Sen 《Plant Breeding》1998,117(2):182-184

In-gel hybridization patterns were studied in a set of nine diverse bread wheat ( Triticum aestivum L. em. Thell) genotypes using 23 simple sequence repeat (SSR) probes in combination with 14 different restriction enzymes. Multilocus fingerprints due to SSR probes, shown earlier to be characteristic of a majority of plant genomes, were not obtained and only a very low level of polymorphism was detected when using as many as 142 probe-enzyme combinations. The hybridization of a prominent solitary high molecular weight fragment (> 23 kb) with a number of SSR probes suggested the presence of these SSRs (microsatellites) within the long stretches of repeated DNA sequences. This indicates that the genome of bread wheat differs from that of other plants in the organization and distribution of SSRs and that SSR probes detect very little polymorphism.  相似文献   

Capturing genetic variability and selection of traits for heat tolerance in a chickpea recombinant inbred line (RIL) population under field conditions     

Pronob J. Paul  Srinivasan Samineni  Sobhan B. Sajja  Abhishek Rathore  Roma R. Das  Sushil K. Chaturvedi  G. R. Lavanya  Rajeev K. Varshney  Pooran M. Gaur 《Euphytica》2018,214(2):27

Chickpea is the most important pulse crop globally after dry beans. Climate change and increased cropping intensity are forcing chickpea cultivation to relatively higher temperature environments. To assess the genetic variability and identify heat responsive traits, a set of 296 F_8–9 recombinant inbred lines (RILs) of the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant) was evaluated under field conditions at ICRISAT, Patancheru, India. The experiment was conducted in an alpha lattice design with three replications during the summer seasons of 2013 and 2014 (heat stress environments, average temperature 35 °C and above), and post-rainy season of 2013 (non-stress environment, max. temperature below 30 °C). A two-fold variation for number of filled pods (FPod), total number of seeds (TS), harvest index (HI), percent pod setting (%PodSet) and grain yield (GY) was observed in the RILs under stress environments compared to non-stress environment. A yield penalty ranging from 22.26% (summer 2013) to 33.30% (summer 2014) was recorded in stress environments. Seed mass measured as 100-seed weight (HSW) was the least affected (6 and 7% reduction) trait, while %PodSet was the most affected (45.86 and 44.31% reduction) trait by high temperatures. Mixed model analysis of variance revealed a high genotypic coefficient of variation (GCV) (23.29–30.22%), phenotypic coefficient of variation (PCV) (25.69–32.44%) along with high heritability (80.89–86.89%) for FPod, TS, %PodSet and GY across the heat stress environments. Correlation studies (r = 0.61–0.97) and principal component analysis (PCA) revealed a strong positive association among the traits GY, FPod, VS and %PodSet under stress environments. Path analysis results showed that TS was the major direct and FPod was the major indirect contributors to GY under heat stress environments. Therefore, the traits that are good indicators of high grain yield under heat stress can be used in indirect selection for developing heat tolerant chickpea cultivars. Moreover, the presence of large genetic variation for heat tolerance in the population may provide an opportunity to use the RILs in future-heat tolerance breeding programme in chickpea.  相似文献   

Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc     

Simi Jacob  Ramgopal Rao Sajjalaguddam  K. Vijay Krishna Kumar  Rajeev Varshney  Hari Kishan Sudini 《Journal of General Plant Pathology》2016,82(2):96-104

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Vernalization response in chickpea is controlled by a major QTL     

Srinivasan Samineni  Suresh Kamatam  Mahendar Thudi  Rajeev K. Varshney  Pooran M. Gaur 《Euphytica》2016,207(2):453-461

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Foliar fungal disease‐resistant introgression lines of groundnut (Arachis hypogaea L.) record higher pod and haulm yield in multilocation testing          下载免费PDF全文

Janila Pasupuleti  Manish K. Pandey  Surendra S. Manohar  Murali T. Variath  Premalatha Nallathambi  Hajisaheb L. Nadaf  Harikishan Sudini  Rajeev K. Varshney 《Plant Breeding》2016,135(3):355-366

Introgression lines (ILs) of groundnut with enhanced resistance to rust and late leaf spot (LLS) recorded increased pod and haulm yield in multilocation testing. Marker‐assisted backcrossing (MABC) approach was used to introgress a genomic region containing a major QTL that explains >80% of phenotypic variance (PV) for rust resistance and 67.98% PV for LLS resistance. ILs in the genetic background of TAG 24, ICGV 91114 and JL 24 were evaluated for two seasons to select 20 best ILs based on resistance, productivity parameters and maturity duration. Multilocation evaluation of the selected ILs was conducted in three locations including disease hot spots. Background genotype, environment and genotype × environment interactions are important for expression of resistance governed by the QTL region. Six best ILs namely ICGV 13192, ICGV 13193, ICGV 13200, ICGV 13206, ICGV 13228 and ICGV 13229 were selected with 39–79% higher mean pod yield and 25–89% higher mean haulm yield over their respective recurrent parents. Pod yield increase was contributed by increase in seed mass and number of pods per plant.  相似文献