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Md. Shaheed Reza Nanami Mizusawa Ayaka Kumano Chiharu Oikawa Daisuke Ouchi Atsushi Kobiyama Yuichiro Yamada Yuri Ikeda Daisuke Ikeda Kazuho Ikeo Shigeru Sato Takehiko Ogata Toshiaki Kudo Mitsuru Jimbo Ko Yasumoto Kazutoshi Yoshitake Shugo Watabe 《Fisheries Science》2018,84(3):563-577
In an effort to determine genus- or species-level taxonomic profiles and diversity of bacterial consortia in the Tama River around urban Tokyo, next-generation sequencing technology targeting a 16S ribosomal RNA (rRNA) gene amplicon was employed. Metagenomic analysis performed by an Ion Personal Genome Machine after sequentially filtering samples through 5-, 0.8- and 0.2-μm filters yielded 1.48 Gb of 16S sequences (average 2.38 M reads/sample). The results indicated that half of the bacterial sequences belonged to Proteobacteria, followed by Bacteroidetes, Actinobacteria and Cyanobacteria. Flavobacterium (Bacteroidetes), possibly including a potential fish pathogen, was the most numerous genera in the Tama River metagenome, and accounted for?~?16% of assigned 16S reads, followed by Mycobacterium. Other dominant bacterial genera including Zoogloea, Sediminibacterium, Hyphomicrobium, Sphingopyxis, Thiothrix and Lysobacter, were thought to be associated with waste water and sludge. MiSeq metagenomic analysis revealed that environmental factors, particularly water temperature, influenced the bacterial composition throughout the year, with a strong negative correlation observed for Proteobacteria and a positive correlation for Bacteroidetes. In terms of bacterial genera, Flavobacterium was positively correlated with temperature, while Polaromonas, Pseudomonas and Bradyrhizobium were negatively correlated with this, suggesting dynamic change in the free-living bacterial population throughout the year and versatile adaptation strategies in relation to environmental factors. 相似文献
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Miyuki?NittaEmail author Ju?Kyong?Lee Churl?Whan?Kang Masumi?Katsuta Satoko?Yasumoto Dajun?Liu Tsukasa?Nagamine Ohmi?Ohnishi 《Genetic Resources and Crop Evolution》2005,52(7):797-804
Perilla (Lamiaceae) contains one tetraploid species, P. frutescens (L.) Britt. and three diploid species, P. citriodora (Makino) Nakai, P. hirtella Nakai and P. setoyensis G. Honda. Tetraploid species have been traditionally cultivated in Asia for their seed oil and for their fragrant leaves
that are used as medicine or as a garnish for fish. The center of diversity is still obscure. To conserve the genetic resources,
it is important to know the diversity of the tetraploid species. The three diploid species, which are possible parents of
the tetraploid species, are all believed to be indigenous to Japan. Their distribution in China and Korea was clarified on
the basis of herbarium and field surveys. The tetraploid species is assumed to have originated somewhere around the mid-to
downstream area of the Changjiang River. Though Perilla is not cultivated as often in these areas as in northern China, Korea, the Himalayan region, or Myanmar, these areas should
also be important for the conservation of genetic resources of tetraploid Perilla crops because of the expected high genetic diversity. 相似文献
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R. Nozaki M. Takushima K. Mizuno K. Kadomura S. Yasumoto K. Soyano 《Fish physiology and biochemistry》2003,28(1-4):217-218
The reproductive cycle of devil stinger was investigated by histological observation and hormone analysis. The gonadal development of devil stinger starts between January and February. Spawning season of the fish is from May to August, with peaks from May to June. 相似文献
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Toshiyuki?SuzukiEmail author Tomoji?Igarashi Kazuhiko?Ichimi Masatoshi?Watai Megumi?Suzuki Eri?Ogiso Takeshi?Yasumoto 《Fisheries Science》2005,71(4):948-955
ABSTRACT: Four toxins, okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-6 (PTX6), and yessotoxin (YTX), all associated with diarrhetic shellfish poisoning (DSP), were administered via syringe to Scallops Patinopecten yessoensis and their distribution in the hepatopancreas, adductor muscle, and combined other tissues (mantle, gill, gonad) was analyzed by liquid chromatography-mass spectrometry. Toxins exclusively remained in the hepatopancreas irrespective of the injection site, adductor muscle or hepatopancreas. When injected into hepatopancreas, OA, DTX1, and YTX were metabolized to 7- O -palmitoylOA, 7- O -palmitoylDTX1 and 45-hydroxyyessotoxin (45OH-YTX), respectively. Such metabolic changes were insignificant when toxins were injected into the adductor muscle. The residual ratio for each toxin in the hepatopancreas was less than 20%. Mortalities of scallops treated with PTX6 were lower than those treated with other toxins. 相似文献
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Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses 总被引：1，自引：0，他引：1
Maeda K Yasumoto S Tsuruda A Andoh K Kai K Otoi T Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(9):989-991
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses. 相似文献
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Ryota Takeuchi Mitsuru Jimbo Fumika Tanimoto Mariko Iijima Hiroshi Yamashita Go Suzuki Saki Harii Yoshikatsu Nakano Ko Yasumoto Shugo Watabe 《Marine drugs》2021,19(3)
Many corals establish symbiosis with Symbiodiniaceae cells from surrounding environments, but very few Symbiodiniaceae cells exist in the water column. Given that the N-acetyl-d-glucosamine-binding lectin ActL attracts Symbiodiniaceae cells, we hypothesized that corals must attract Symbiodiniaceae cells using ActL to acquire them. Anti-ActL antibody inhibited acquisition of Symbiodiniaceae cells, and rearing seawater for juvenile Acropora tenuis contained ActL, suggesting that juvenile A. tenuis discharge ActL to attract these cells. Among eight Symbiodiniaceae cultured strains, ActL attracted NBRC102920 (Symbiodinium tridacnidorum) most strongly followed by CS-161 (Symbiodinium tridacnidorum), CCMP2556 (Durusdinium trenchii), and CCMP1633 (Breviolum sp.); however, it did not attract GTP-A6-Sy (Symbiodinium natans), CCMP421 (Effrenium voratum), FKM0207 (Fugacium sp.), and CS-156 (Fugacium sp.). Juvenile polyps of A. tenuis acquired limited Symbiodiniaceae cell strains, and the number of acquired Symbiodiniaceae cells in a polyp also differed from each other. The number of Symbiodiniaceae cells acquired by juvenile polyps of A. tenuis was correlated with the ActL chemotactic activity. Thus, ActL could be used to attract select Symbiodiniaceae cells and help Symbiodiniaceae cell acquisition in juvenile polyps of A. tenuis, facilitating establishment of symbiosis between A. tenuis and Symbiodiniaceae cells. 相似文献
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Satoko Yasumoto Natsumi Maki Makoto Kojima Yasuo Ohshita 《Plant Production Science》2017,20(3):279-287
We investigated changes in the developmental duration of rice cultivars with a wide range of maturities in response to late planting. Elucidating the relationship between cropping season and the progress of growth stages is important for establishing direct-seeded cultivation and creating growth models. Late planting of the intermediate to late maturing cultivars Akidawara and Hoshijirushi decreased the time from emergence to panicle formation and decreased the cumulative effective temperature (CET) and cumulative effective soil temperature (CEST). In the very early maturing cultivars Ichibanboshi and Fusakogane, the changes in number of days, CET, and CEST from emergence to panicle formation with late planting were small. From emergence to heading and maturity, the number of days, CET, and CEST of many cultivars tended to increase until mid-May and then decreased. The changes in the number of days, CET, and CEST caused by late planting were greater for the intermediate to late maturing cultivars than for the very early maturing cultivars. The differences between cultivars were greatest with early May sowing, and then decreased with later planting. Short-day condition revealed significant differences in the duration of vegetative growth and CET among cultivars, but long-day condition erased these differences. These results demonstrate that the photosensitivity and thermosensitivity of cultivars are especially important in crop planning and for creating growth models of direct-seeded rice. 相似文献
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Tomioka Keisuke Asami Hidenori Chiba Masahiro Kobayashi Hidekazu Nagata Kenji Mori Shinsuke Yamazaki Ryo Kawasaki Yohei Yasumoto Satoko Masunaka Akira Sekiguchi Hiroyuki Ishikawa Naoyuki 《Journal of General Plant Pathology》2021,87(5):281-286
Journal of General Plant Pathology - Brown lesions were found on leaves of the weed barnyardgrass in paddy fields in Hiroshima Prefecture in western Japan in June 2017. A fungus, isolated... 相似文献
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Masuda T Koseki SY Yasumoto K Kitabatake N 《Journal of agricultural and food chemistry》2000,48(7):2670-2674
Five monoclonal antibodies (OVA-01, -02, -03, -04, -06) produced against irradiated ovalbumin were investigated in relation to the conformational change in the ovalbumin molecule induced by irradiation with Cobalt-60 gamma-rays. Four antibodies (OVA-01, -02, -04, -06) recognized both native and irradiated ovalbumin, but OVA-03 reacted only with irradiated ovalbumin. These antibodies were classified by modified competitive ELISA, and their K(d) values were determined by the Klotz equation. Epitope analyses were also performed on OVA-03 using CNBr-cleaved peptide fragments from ovalbumin, and it was confirmed that OVA-03 bound to the fragment corresponding to residues Val173-Met196 of the ovalbumin molecule that consists of internal beta-sheet strand 3A and helix F1 containing one open turn. These results demonstrate that dramatic conformational changes in proteins can be induced or that some tertiary or secondary structures can be broken down by gamma-ray irradiation, producing new antigenic sites. 相似文献