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Evaluation of a Killed Vaccine Against Porcine Pleuropneumonia Due to Haemophilus pleuropneumoniae 总被引:20,自引:2,他引:18
Higgins R Larivière S Mittal KR Martineau GP Rousseau P Cameron J 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(2):86-89
A bacterin containing serotypes 1 and 5 of Haemophilus pleuropneumoniae was developed for the prevention and the control of porcine pleuropneumonia. It was injected intramuscularly into three groups of ten piglets, the first group with one dose, the second one with two doses and the third one with three doses at two-week intervals. Another group of ten piglets did not receive the vaccine. All the piglets were then challenged by an aerosol of mixed suspensions of H. pleuropneumoniae serotypes 1 and 5. Two and three injections of vaccine completely prevented mortality, whereas half of the control piglets and of those receiving only one dose of vaccine died. All surviving piglets, both control and vaccinated, had severe signs of respiratory disease for at least 36 hours after exposure to challenge. Moreover, vaccination did not induce the production of antibodies at high titers. Local reactions were not noted after vaccination and at postmortem; ten weeks after the challenge, there were no signs of abscess formation or induration. 相似文献
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Although often seen as a scale-independent measure, we show that the fractal dimension of the forest cover of the Cazaville Region changes with spatial scale. Sources of variability in the estimation of fractal dimensions are multiple. First, the measured phenomenon does not always show the properties of a pure fractal for all scales, but rather exhibits local self-similarity within certain scale ranges. Moreover, some sampling components such as area of sampling unit, the use of a transect in the estimation of the variability of a plane, the location, and the orientation of a transect all affect, to different degrees, the estimation of the fractal dimension. This paper assesses the relative importance of these components in the estimation of the fractal dimension of the spatial distribution of woodlots in a fragmented landscape. Results show that different sources of variability should be considered when comparing fractal dimensions from different studies or regions. 相似文献
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During 1972 to 1974, 112 Escherichia coli strains isolated from diarrheic piglets were recieved from different parts of the Province of Quebec, Canada. Fifty-six strains elicited a positive gut loop response in three week old piglets and were then considered as Moon's class 1 enteropathogens, while four of the 56 remaining strains reacted only in ten day old piglets and were classified as class 2 enteropathogens. Forty-eight strains produced both a heat-labile and a heat-stable enterotoxin, while 12 isolates which included the four class 2 enteropathogens produced only a heat-stable enterotoxin. Fifty-one enterotoxigenic strains could be serogrouped using OK antisera against E. coli strains commonly associated with colibacillosis in piglets. The most common serogroups encountered were O157: K "V17"; 88a,c, O149:K91; 88a.c. O157:K"V1c, O149:K91; 88a.c, O157:K"V17"; 88 a,c or a,b and O45:K"E5"; 88a,c. No significant difference was observed in the fermentation patterns, antibiotic susceptibility, colicin production, production of a filterable hemolysin and transferable tetracycline resistance between the enterotoxigenic and the nonenterotoxigenic strains. 相似文献
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Functional brain alterations induced by lindane were examined in Rat chronically exposed to low doses of lindane (1 and 2 μg L−1 in drinking water) from conception. Histological and electrophysiological investigations were conducted at 14 weeks of age. Lindane did not induce histological changes. It did not significantly modify the sleep-wakefulness cycle and EEG did not show major alterations. The spectral EEG analysis, by recording cortical variations in both energy spectrum and energy levels of different frequency bands, showed an increase in the 11-15 Hz activity in both groups exposed to lindane. This activity was characterised by spindles well-depicted during slow wave sleep or associated with fast activity during wakefulness and theta activity in paradoxical sleep. These data suggest that chronic exposure to lindane doses as low as 1 μg L−1 in drinking water could lead to central electrophysiological effects possibly involving GABA-benzodiazepine mechanisms. 相似文献
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Bérénice Agnola Stéphane Boury Claudie Monot Anne Quillévéré Yves Hervé Drissa Silué 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(5):471-478
A rapid resistance/susceptibility test for Peronospora parasitica (downy mildew) was established by inoculating leaf-disks of four Brassica oleracea accessions. Several conditions were tested: disk disinfection or not, agar medium with or without nutrients and with 50 or 100 ppm of benzimidazole. Using disinfected disks placed on agar (no nutrient and benzimidazole at 50 or 100 ppm), the responses of leaf-disks to four isolates were similar to those obtained using the classical cotyledon test, whereas undesired contaminations occurred in all other conditions. The possible effect of the particular leaf used for obtaining the disks was also studied. In each incompatible interaction tested, disks were resistant whatever the leaf used. In compatible interactions, susceptible phenotypes were observed on disks derived from the six lowest leaves, but disks from upper leaves were resistant. The genetic basis of resistance in a F1 hybrid broccoli was assessed, by testing six isolates on an F2 population derived from this hybrid. The cotyledon test only allows inoculation of two isolates per seedling, whereas many isolates can be tested on each plant by using leaf-disks. The segregation of the resistance to each of the six isolates was analysed: two dominant genes (tightly linked) control resistance to all isolates (one to five isolates; the other to only one isolate). 相似文献