Breakdown of resistance in cotton to cotton leaf curl disease in Pakistan   总被引：5，自引：0，他引：5  

S. Mansoor  I. Amin  S. Iram  M. Hussain  Y. Zafar  K. A. Malik  R. W. Briddon 《Plant pathology》2003,52(6):784-784

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First report of Mungbean yellow mosaic India virus on mungbean in Pakistan     

M. Hussain  J. Qazi  S. Mansoor  S. Iram  M. Bashir  Y. Zafar 《Plant pathology》2004,53(4):518-518

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Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)     

S. Ali  M. Ijaz  S. H. Farooqi  A. Z. Durrani  M. I. Rashid  A. Ghaffar  A. Ali  A. Rehman  S. Aslam  I. Khan  A. Masud  K. Mehmood 《Equine Veterinary Education》2021,33(2):75-83

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.  相似文献   

Effect of nitrogen deficiency on growth and some biochemical characteristics in salt‐tolerant and salt‐sensitive lines of Lentil (lens cul1naris medic.)     

M. Ashraf  Z.U. Zafar 《Archives of Agronomy and Soil Science》2013,59(4):231-239

The response of a salt‐tolerant line ILL 6793 and a salt‐sensitive line ILL 6439 of lentil (Lens culinaris) to N deficiency was studied in a pot experiment under glasshouse conditions. Plants of the two lines were treated with 56, 28, 14 and 7 mg N L^?1 in Rorison's nutrient solution. The salt‐tolerant line excelled the salt‐sensitive line in relative biomass production (per cent of control basis) under varying N regimes. Of the various physiological/biochemical variables measured in the present study only chlorophyll b and total soluble sugars proved to be helpful in discriminating the lines. Chlorophyll b was significantly greater in ILL 6793 as compared with ILL 6439 at the two lower N levels. Total soluble sugars increased consistently in both lines with decrease in N level of the growth medium and the salt‐tolerant line ILL 6793 had significantly greater soluble sugars than the salt‐sensitive ILL 6439 at 28 and 14 mg N L^?1. Chlorophyll a and free amino acids were uniformly reduced in both lines with decrease in N levels. The salt‐tolerant line showing high efficiency for N utilization could be of great economic value in terms of its use in salt‐affected soils which are usually deficient in N provided it also produces reasonable grain yield.  相似文献   

Molecular simulations of Taxawallin I inside classical taxol binding site of β-tubulin     

Khan I  Nisar M  Ahmad M  Shah H  Iqbal Z  Saeed M  Halimi SM  Kaleem WA  Qayum M  Aman A  Abdullah SM 《Fitoterapia》2011,82(2):276-281

A new taxoid Taxawallin I (1) along with two known taxoids (2-3) were isolated from methanolic bark extract of Taxus wallichiana Zucc. Structural characterization was confirmed by mass and NMR spectral techniques. Taxawallin I exhibited significant in-vitro anticancer activity against HepG2, A498, NCI-H226 and MDR 2780AD cancer lines. Tubulin binding assay was performed to assess its tubulin binding activity. Molecular docking analysis was performed to study the potential binding mode inside the taxol binding site of β-tubulin.  相似文献   

Inulin effects on bioavailability of soy isoflavones and their calcium absorption enhancing ability     

Zafar TA  Weaver CM  Jones K  Moore DR  Barnes S 《Journal of agricultural and food chemistry》2004,52(10):2827-2831

The effect of inulin on isoflavone absorption and the effect of isoflavones and synergy with inulin on calcium absorption in rats was investigated. Rats (n = 48) were divided into three groups and fed inulin (50 mg/g), isoflavone (8 mg/g) or inulin + isoflavone (50 mg/g + 8 mg/g) diets for 21 days. After a 2-h fast, rats were given (45)Ca orally or intraperitoneally, together with 25 mg of calcium as calcium acetate. Blood and femurs were collected 4 days later. Sera were analyzed for isoflavones using HPLC-MS, femurs for (45)Ca by beta-scintillation counting, and total femoral calcium by atomic absorption spectroscopy. Both groups fed isoflavones had similar and significantly higher weight-adjusted total femoral calcium content compared to the inulin-fed group (p < 0.0001). (45)Ca absorption was significantly higher (p < 0.01) when isoflavones were added to the diet, and serum equol was significantly lower (p < 0.01) when inulin was added to the diet containing isoflavones. We conclude that isoflavones enhance calcium absorption, without synergy from inulin, and that inulin decreases equol production.  相似文献   

Dynamic shifts of limited working memory resources in human vision     

Bays PM  Husain M 《Science (New York, N.Y.)》2008,321(5890):851-854

Our ability to remember what we have seen is very limited. Most current views characterize this limit as a fixed number of items-only four objects-that can be held in visual working memory. We show that visual memory capacity is not fixed by the number of objects, but rather is a limited resource that is shared out dynamically between all items in the visual scene. This resource can be shifted flexibly between objects, with allocation biased by selective attention and toward targets of upcoming eye movements. The proportion of resources allocated to each item determines the precision with which it is remembered, a relation that we show is governed by a simple power law, allowing quantitative estimates of resource distribution in a scene.  相似文献   

Comment on "Detecting awareness in the vegetative state"     

Nachev P  Husain M 《Science (New York, N.Y.)》2007,315(5816):1221; author reply 1221

Owen et al. (Brevia, 8 September 2006, p. 1402) claimed that a patient's brain activity revealed that she was consciously responding to commands despite being in a vegetative state. However, several alternative explanations were not eliminated. Specifically, the activity could reflect unconscious reactions to the last word in the command, not conscious decisions to respond. A refined experimental design could clarify these issues.  相似文献   

Physico-chemical characteristics of apricot (Prunus armeniaca L.) grown in Northern Areas of Pakistan     

Sartaj Ali  Tariq Masud  Kashif Sarfraz Abbasi 《Scientia Horticulturae》2011

In order to ascertain physico-chemical, functional and geometrical traits of apricot fruit from Northern Areas of Pakistan, six predominantly grown varieties namely, Alman, Habi, Khakhas, Mirmalik, Neeli and Shai were selected in this study. Proximate composition as crude fat (2.1–3%), crude protein (6.18–8.7%), crude fiber (11.85–13.6%), ash (9.45–12.1%) and total sugars (56.8–64.9%) were determined on dry weight basis. The data showed variations among the investigated parameters in all varieties. Functional properties of apricot fruit viz. ascorbic acid (67.39–90.94 mg/100 g), total phenolic compounds (4590–7310 mgGAE/100 g), total carotenoids (10.09–18.13 mg/100 g β-carotene) and antioxidant activity (56.84–82.33%) were also recorded. The data pertaining to mineral contents (mg/100 g) revealed K as the predominant element (2040–3000) followed by P, Mg, Ca, Na and Fe among all the tested samples. Furthermore, geometrical characters of apricot varieties were also determined as important sensory and technological attributes on fresh weight basis. The result from the present study showed that all the tested varieties are highly nutritious and rich in functional components.  相似文献   

Gel filtration clean-up for multi-residues of organophosphorus pesticides in rice     

Z. Masud  V. Btora  J. Kova i ov 《Pest management science》1973,4(1):131-136

Gel filtration on Bio-Beads S-X8 and Sephadex LH-20 columns has been successfully applied to the clean-up of multiple residues of malathion, fenitrothion and dichlorvos in rice. Recoveries were checked by gas-liquid chromatography using CsBr-thermionic detector and ranged from 77 to 104% at 5 mg/kg level. The clean-up was found to be highly effective. No interference was observed in the gas chromatograms.  相似文献