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Balamurugan Vinayagamurthy Alamuri Anusha Bharathkumar K. Patil Sharanagouda Siddanagouda Govindaraj Gurrappa Naidu Nagalingam Mohandoss Krishnamoorthy Paramanandham Rahman Habibur Shome Bibek Ranjan 《Tropical animal health and production》2018,50(5):1131-1138
Tropical Animal Health and Production - In this study, the seroprevalence and distribution of Leptospira in dairy cattle in endemic states of India were investigated in association with... 相似文献
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Polymerase chain reaction (PCR) was used to amplify the spacer regions between the 16S and 23S genes of rRNA genetic loci of Salmonella serovars for their rapid identification. These genetic loci revealed a significant level of polymorphism in length across the species/serovar lines. When the 16S-23S spacer region amplification products were subjected to agarose electrophoresis, the patterns observed could be used to distinguish all the serovars of Salmonella tested. Unique elements obtained in amplification products were mostly clustered at serovar level, although certain genus-specific patterns were also observed. On the basis of the results obtained, the amplification of 16S-23S ribosomal spacer region could suitably be used in a PCR-based identification method for Salmonella serovars. 相似文献
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Bhaskar Choudhury 《Agricultural and Forest Meteorology》1985,34(4):333-336
Well-watered crops having nearly identical ratio of intercept and slope of the “non-water-stressed baseline” would attain their equivalence point temperature at roughly identical air vapor pressure deficit. However, such a behavior of these crops might not prevail as the wet soil dries out progressively. The methods for calculating the equivalence point temperature due to Linacre (1964) and Paw U (1984) gave results which sometimes differed by more than 10 K. 相似文献
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Bhaskar Sharma B. S. Negi A. B. Pandey S. K. Bandyopadhyay H. Shankar M. P. Yadav 《Tropical animal health and production》1988,20(2):109-113
Summary The counterimmunoelectrophoresis (CIE) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. The chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. The precipitinogens of goat pox virus (GPV) were found to be soluble in nature. The CIE test was quick as well as more sensitive than the agar gel precipitation test for detection of GPV antibody/antigen. The CIE employing inactivated antigen has been used for the first time in the detection of GPV antibodies/antigens.
Deteccion Del Antigeno Y Anticuerpos De Viruela Caprina Mediate La Prueba De Contrainmunoelectroforesis
Resumen Se estandarizó la prueba de contrainmunoelectroforesis, para la detección del antígeno y anticuerpos del virus de la viruela caprina, usando antígenos inactivados. El antígeno inactivado con cloroformo y el antígeno vivo, fueron igualmente sensitivos para la detección de precipitinas de viruela caprina. Los precipitógenos del virus de la viruela caprina se encontraron que eran solubles. La prueba de contrainmunoelectroforesis fue más rápida y más sensitiva que la precipitación agar gelatina para la detección de anticuerpos/antígenos del virus de la viruela caprina. La prueba de contrainmunoelectroforesis con antígeno inactivado ha sido utilizada por vez primera en la detección de anticuerpos/antígenos del virus de la viruela caprina.
Detection De l'Antigene Et De l'Anticorps Variole Caprine Par Un Test De Contrimmuno-Electrophorese
Résumé Le test de contrimmuno-électrophorèse (CIE) a été standardisé pour la détection de l'antigène et de l'anticorps variole caprine avec des antigènes inactivés. Les antigènes vivants et inactivés par le chloroforme sont de sensibilité équivalente pour la détection des précipitines variole caprine. On a montré que ces précipitogènes du virus variole caprine (VVC) étaient de nature soluble. Le CIE est rapide et plus sensible que le test de précipitation en gélose pour la détection des antigènes et anticorps VVC. C'est la première fois que le CIE mettant en oeuvre un antigène inactivé a été utilisé.相似文献
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V. S. A. K. Sama K. Himabindu S. Bhaskar Naik R. M. Sundaram B. C. Viraktamath J. S. Bentur 《Euphytica》2012,187(3):393-400
Host plant resistance is the preferred management strategy for Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. Identification of simple sequence repeat (SSR) markers that are tightly linked to pest resistance genes can accelerate development of gene pyramids for durable/multiple resistance. Based on conventional and molecular allelism tests, we report herein that rice genotype Aganni possesses Gm8 gene, conferring hypersensitive independent (HR– type) resistance to gall midge biotypes GMB1, GMB2, GMB3, GMB4, and GMB4M. The gene Gm8 was mapped to chromosome 8 within a 400-kbp region, and the SSR markers RM22685 and RM22709 flank the gene closely. Using these closely linked flanking markers, nine other gall midge-resistant genotypes were identified as carrying the same gene Gm8. Through marker-assisted selection, Gm8 has been introgressed into an elite bacterial blight-resistant cultivar, Improved Samba-Mahsuri (IS). 相似文献
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Shanti Shrestha Bhaskar Singh Karky Anup Gurung Rajesh Bista Ole Reidar Vetaas 《Small-Scale Forestry》2013,12(4):507-517
This study assessed the net above-ground carbon stock in six community forests in the Dolakha district, Nepal. A survey was conducted of above-ground timber species, using random sampling. A tree-ring chronology for Pinus roxburghii was created to construct a growth model representative of the various mainly-pine species. The allometric model combined with tree ring analysis was used to estimate carbon stock and annual growth in the above-ground tree biomass. The out-take of forest biomass for construction material and fuelwood was estimated on the basis of interviews and official records of community forest user groups. The average annual carbon increment of the community forests was 2.19 ton/ha, and the average annual carbon out-take of timber and fuelwood was 0.25 ton/ha. The net average carbon balance of 1.94 ton/ha was equivalent to 117.44 tons of carbon per community forest annually. All the community forests were actively managed leading to a sustainable forest institution, which acts as a carbon sink. It is concluded that community forests have the potential to reduce emissions by avoiding deforestation and forest degradation, enhance forest carbon sink and improve livelihoods for local communities. 相似文献
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