Effects of music stimulus on behavior response,cortisol level,and horizontal immunity of growing pigs     

Jiafang Li  Xiang Li  Honggui Liu  Jianhong Li  Qian Han  Chao Wang  Xiangyin Zeng  Yutao Li  Wenbo Ji  Runxiang Zhang  Jun Bao 《Journal of animal science》2021,99(5)

An enriched environment is widely used to improve domestic animals’ welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs.  相似文献   

云南省部分养殖场鸡传染性贫血病毒核酸检测及VP2基因序列分析     

王斌  张振兴  宋建领 《中国动物检疫》2021,38(10):27-29

为了解云南省鸡传染性贫血病毒（chicken anemia virus，CAV）的流行及其VP2基因变异情况，2017—2020年，在昆明市、楚雄州、大理州、红河州、玉溪市等家禽养殖密集区的89个鸡场，采集422份疑似病鸡肝脏样品，通过PCR方法检测CAV核酸。结果显示，检出阳性245份，平均阳性率为58.06%；不同年份的CAV核酸阳性率为53.61%~60.91%，不同区域核酸阳性率为51.68%~63.52%。对3份CAV阳性样品进行VP2基因序列分析，发现核苷酸序列同源性为99.6%~99.8%，与参考毒株1852TW和N7的核苷酸同源性为99.6%~99.8%，均属于GroupA分支。结果表明：云南省普遍存在CAV感染，且感染较严重；VP2基因仍可作为PCR病毒核酸检测的首选目标基因。通过加强鸡群CAV监测，淘汰阳性鸡群和结合疫苗免疫，可减少该病造成的损失。  相似文献   

东北宿根花卉在花海建设中的应用——以莲花山花海项目为例     

张晓丽  陈亚林  程彬  杨帆  朱春英  于春波 《吉林林业科技》2021,50(5):30-33

本文以莲花山南北向花海的东北宿根花卉种植为例,介绍了东北宿根花卉在花海及园林绿化工程中的应用,并总结了在施工过程中遇到的问题,为今后花海的景观设计及施工提供思路和方向.  相似文献   

特早熟抗病棉花品种辽棉35选育及栽培技术     

王巍  赵志强  高艳平  吴晓东  韩晓军  王子胜 《中国棉花》2018,45(12):37-38

本文主要介绍辽棉35的选育过程、生物学特性、产量、纤维品质、抗病性及栽培技术要点。  相似文献   

日光温室新型装配式骨架节点设计与有限元分析   总被引：3，自引：0，他引：3  

周凤  何斌 《北方园艺》2021,(12):50-56

为实现日光温室骨架的装配化,把控安装质量,提高温室骨架受力性能,设计完成了日光温室新型装配式骨架及装配式节点,以期为装配式日光温室提供新的研究思路.利用ANSYS Workbench软件建模辅助分析,比选外套管和内插管2种装配方式,在逐级均布荷载作用下主管和套管所受应力、剪力及弯矩情况;用Workbench软件分析装配式骨架在最不利荷载作用下轴向应力、剪力、弯矩及变形情况.结果 表明:骨架在外套管的装配方式下,主管及套管自身所受的轴向应力和弯矩更小,安全性更高.用外套管装配的三段桁架在最不利荷载组合作用下,最大轴向应力17.35MPa,最大变形量为0.26 mm,套管最大轴向应力5.0 MPa,最大剪力2345.3 N,最大弯矩为41.3N·m.该日光温室新型装配式骨架及装配式节点安全、稳定、变形量小,可推广使用.  相似文献   

4个甜樱桃品种在德州的引种表现及设施栽培技术   总被引：1，自引：0，他引：1  

谭延肖  郑现和  徐文胜  韩梅梅 《落叶果树》2020,52(3):33-35

对引入山东德州的4个甜樱桃品种美早、萨米脱、布鲁克斯、拉宾斯进行了日光温室栽培试验,对其性状表现进行了观察。结果表明,4个品种均表现生长良好,适应性强,结果正常,果实品质优良,可以作为德州地区日光温室的栽培品种发展。简述了4个甜樱桃品种在日光温室的栽培技术。  相似文献   

利用SOM聚类分析及主成分分析方法鉴定小麦品种萌发期耐盐性     

韩丽萍  郭卫卫  韩晓凡  李夕梅  王会芳  张玉梅  仇宏伟 《中国农学通报》2021,37(24):6-17

为了研究不同小麦品种萌发期的耐盐性,为小麦萌发期耐盐性鉴定提供快速、准确的鉴定方法和理论依据。本研究以40个冬小麦品种为试验材料,通过主成分分析法(PCA)与神经网络自组织映射(SOM)聚类分析法,研究盐胁迫对40个小麦品种萌发期各项形态指标的影响。主成分分析法结果表明,发芽势、发芽率、最长根长、第一片叶长及胚芽鞘长在PCA下载荷量最大;根据SOM聚类分析结果,‘德抗961’等4个小麦品种属于高度耐盐品种,‘济麦22’等14个小麦品种属于耐盐性品种,‘济麦20’等16个品种属于中等耐盐性品种,‘鲁麦21’等6个小麦品种属于盐敏感性品种。发芽势、发芽率、最长根长、第一片叶长、胚芽鞘长能够作为萌发期小麦耐盐性筛选的主要评价指标,并且结合主成分、SOM聚类分析方法进行小麦萌发期耐盐性鉴定更为科学、方便。  相似文献   

Three SNPs within exons of INHA and ACVR2B genes are significantly associated with litter size in Dazu black goats     

Shi-Zhi Wang  Guang-Xin E  Yan Zeng  Yan-Guo Han  Yong-Fu Huang  Ri-Su Na 《Reproduction in domestic animals》2021,56(6):936-941

The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary.  相似文献   

黔东南州打造全域森林公园的可行性研究     

粟燕  刘人源  覃斌  李权 《绿色科技》2020,(4):152-153,157

指出了建设全域森林公园是经济和社会发展的必然要求。在对黔东南州发展全域森林公园进行必要性和打造条件后认为:黔东南州在打造全域森林公园方面具有明显的政策优势,有着优良的森林生态环境资源,以及良好的林业产业基础等优势条件。通过不断完善相关的配套服务体系,已具备了打造全域森林公园的可行性。  相似文献   

马疱疹病毒1型主要毒力基因分析及TK基因缺失载体的构建     

范斌  陈卓  刘建华  鲍子磊  胡月  贾钦瑞  王雪竹  冉多良 《中国畜牧兽医》2018,45(10):2681-2690

为了解新疆马疱疹病毒1型（EHV-1）主要毒力基因遗传进化情况并构建TK基因缺失株，本研究以EHV-1 XJ2015株DNA为模板，对其主要毒力基因TK、gI和gE全长进行克隆、测序及生物信息学分析，并扩增TK基因左右重组臂TKL和TKR，构建质粒pUC-TKLR，将扩增后的增强绿色荧光蛋白（EGFP，含有CMV+polyA）插入pUC-TKLR质粒，构建TK基因缺失打靶质粒。TK、gI和gE基因同源性分析结果显示，XJ2015株与国外EHV-1分离株TK、gI和gE基因同源性均较高，分别为99.8%~100.0%、99.6%~100.0%和99.9%~100.0%；与EHV-3分离株同源性均最低，分别为72.9%、59.4%和62.1%；遗传进化分析显示，3个基因均与国外EHV-1同属于一个遗传进化分支，与EHV-9和EHV-4进化关系较近，但与EHV-3进化关系较远，表明XJ2015毒株与国外EHV-1毒株TK、gI、gE基因核苷酸上差异不明显，没有明显的地域性特征，功能基因保守且进化缓慢，同源基因功能相同或相近；经PCR扩增、酶切、测序及转染鉴定，本试验成功构建了用于TK基因缺失的打靶质粒pUC-TKLR-EGFP。通过对EHV-1主要毒力基因的分析及TK基因缺失打靶载体的构建，为新疆地区马鼻肺炎流行病学调查分析、TK基因缺失株的构建提供理论依据。  相似文献