High efficient expression of human endostatin in E.coli and its antiangiogensis activity     

ZHANG Ge  KE Xi-song  DING Ai-ling  YING Wei  YANG kun  ZHU Zhen-yu 《园艺学报》2002,18(11):1373-1375

AIM: To examine the expression of human endostatin in E.coli, produce its fusion protein antibody and observe its biological activity. METHODS: Endostatin gene was amplified by polymerase chain reaction,recombined with plasmid vector pGEX-2T and induced expression with IPTG.The protein activity was tested by endothelial cell proliferation inhibitory assay.Inclusion body crudely purified was used to generate polyclonal antibody to detect its expression at mouse's liver and kidney etc. RESULTS: The protein expressed was 20kD after digestion by thrombin,it appeared the anti-angiogenesis activity and Western blotting indicated the expression of endostatin in liver and kidney of mouse. CONCLUSION: The successful expression of human endostatin and the preparation of polycolonal antibody indicated its potential application in anti-angiogenesis therapy and diagnosis tumors.  相似文献   

稻瘿蚊综合防治新技术与示范   总被引：2，自引：0，他引：2       下载免费PDF全文

胡美英  许再福  黄炳球  钟国华  侯任环 《植物保护学报》2003,30(4):401-406

20世纪80年代后，稻瘿蚊发生危害明显回升，损失相当严重。以大田试验示范为主，系统研究了新耕作条件下稻瘿蚊综合防治，首次总结提出“喷药除草、压低虫源、抓住晚秧、做好测报、挑治本田”的综合防治新技术体系。室内外试验和大田示范表明，应用草甘膦杀死越冬寄主游草，可显著减少虫源基数；灭线磷(商品名益舒宝)、氯唑磷(商品名米乐尔)是防治稻瘿蚊的高效药剂，抗蚊青占品种高抗稻瘿蚊。此外，合理利用杀虫植物、性引诱剂和天敌等亦是防治稻瘿蚊的重要措施。在面积达12．46万hm^2的示范推广实践中，本田防治效果均达80％以上。  相似文献   

五种鬼伞过氧化物酶和酯酶的同工酶研究   总被引：2，自引：0，他引：2  

柯丽霞  朱胜东  邵剑文 《中国食用菌》2004,23(4):33-35

应用垂直板聚丙烯酰胺凝胶电泳对五种野生鬼伞 (Coprinus)真菌进行了过氧化物酶 (POD)和酯酶(EST)的同工酶分析 ,结果表明 :五种鬼伞的POD和EST同工酶酶谱比较稳定清晰 ,且分别有一条共同的酶带 ,可能是鬼伞属的POD和EST同工酶特征酶带 ;POD和EST同工酶酶谱均表明 ,家园鬼伞 (C .domesticus)和瓦鳞鬼伞 (C .clavatus)间有较近的亲缘关系 ;不同种鬼伞的POD和EST同工酶之间既有共同的特征 ,又各自有本物种的特有特征 ,POD和EST同工酶酶谱可以作为鬼伞属种类鉴定、亲缘关系比较的重要依据。  相似文献   

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction     

DU Yi-mei  TANG Ming  LIU Chang-jin  HONG Zhi-gang  KE Qin-mei  DI Jiu-fang  LUO Hong-yan  HU Mou-xian  HU Xin-wu  XI Jiao-ya  TANG Bi  Jurgen Hescheler 《园艺学报》2004,20(9):1537-1541

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.  相似文献   

Gly-Gly肽和Gly-L-Leu肽在蛋鸡肠道内水解的研究     

张君涛  刘国华  刘庆生  王建华 《中国家禽》2004,8(Z1):158-160

采用蛋鸡离体外翻肠段培养法,将小肠分为十二指肠、空肠前段、中段、后段和回肠五个部位肠段,洗净外翻。2种二肽培养液(Gly-Gly肽和Gly-L-Leu肽溶液)作为两组对照培养液,每种二肽培养液中添加肽酶抑制剂(Bestatin和Amastatin),作为试验培养液,39.5℃培养25min,每5min取样一次,测定培养液中的二肽含量。结果表明,培养5min后,含Gly-Gly肽的对照和试验培养液中均未能检测到完整Gly-Gly肽。而含Gly-L-Leu肽的对照培养液Gly-L-Leu肽的含量降低到16.75％,试验培养液则下降到43.40％。而试验培养液中Gly-L-Leu肽的含量10min后无显著降低(P>0.05)。各培养时段试验培养液Gly-L-Leu含量均显著高于对照培养液(P<0.05)。由此认为,离体肠囊在培养过程中能够迅速释放肠肽酶及一些生物活性物质,快速水解2种二肽。肽酶抑制剂(Bestatin和Amastatin)能抑制Gly-L-Leu的水解,但不能抑制Gly-Gly的水解。  相似文献   

In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network          下载免费PDF全文

AH Alm‐Kristiansen  ER Gaustad  G Bai  FB Standerholen  G Klinkenberg  E Kommisrud  KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370

Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital^® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital^® (SV) technology, the first commercialized production line of SpermVital^® (C) and by conventional procedure applying Biladyl^® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital^® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen.  相似文献   

广西边境地区活禽批发市场禽流感流行病学监测     

邹联斌  莫胜兰  屈素洁  施开创  陆文俊  尹彦文  李军  邓国华 《中国动物检疫》2018,35(5):1-4

为了给广西边境地区重大动物疫病防控阻截带建设提供数据支持,以及了解中越边境地区家禽禽流感病毒生态学,2012年12月—2017年12月,对广西边境地区主要活禽批发市场家禽及市场环境进行了系统监测。结果显示:广西边境地区活禽批发市场内禽流感病毒污染较重,呈多亚型病毒共存状态;家禽样品的平均病毒分离率为15.95%,环境样品则高达23.65%,明显高于家禽样品。市场调查发现:广西9个边境县(市、区)中,年活禽交易量在100万只以上的有5个;市场交易活禽主要为本地散养或规模场养殖家禽、从其他地区批发转运来的家禽以及边境走私家禽;大部分活禽市场没有配备消毒和无害化处理设施,也未严格执行休市制度。监测和调查结果表明,广西边境地区活禽批发市场家禽交易量大且频繁,来源复杂;市场中禽流感病毒污染严重,多亚型病毒在市场内常年蓄积循环,且存在家禽隐性带毒现象,因而有病毒散播风险。污染严重主要是因市场管理水平不高和生物安全措施不到位造成的,因此需要加强市场的动物卫生监管,提高禽流感监测力度。  相似文献   

菱传粉生物学研究     

李双梅  柯卫东  彭静  黄新芳  李明华 《中国蔬菜》2019,1(8):54-58

以6 个菱品种为试材，对花器形态特征、群体花期及单花开花动态、开花时间、柱头可授性、花粉活力、杂交指数 及传粉形式等进行观察测定。结果表明：菱花单生，两性，单花花期15 h，群体花期在5～10 月，盛花期在7～8 月；不同 品种在一天中的开花高峰期存在差异；柱头可授期主要在萼片开裂后2 h 内，结实率在50% 以上；花粉在自然授粉条件下活 力可保持7.5 h；以自花授粉为主，同时存在一定程度的无融合生殖和少量异花授粉。  相似文献   

氨基酸介导的TOR信号传导通路研究进展     

邓会玲  刘国华  刘宁 《动物营养学报》2011,23(4):529-535

TOR(target of rapamycin)是一种进化上十分保守的丝氨酸(Ser)/苏氨酸(Thr)蛋白激酶,可以感知营养状况、能量、生长因子等信号,进而调节细胞的生长、增殖和凋亡等生理进程.本文综述了TOR的上、下游信号传导通路及各种氨基酸对TOR信号传导通路的影响,为探讨氨基酸调控蛋白质合成的作用机制及建立精确...  相似文献   

一种高质量天门冬基因组DNA提取方法     

韦荣昌  韦树根  付金娥  柯芳  潘丽梅  董青松  唐其 《湖北农业科学》2014,(7):1678-1681

针对天门冬[Asparagus cochinchinensis(Lour.)Merr.]成熟叶片富含多糖、多酚、蛋白质及其他次生代谢物质的特点,采用改良CTAB法对其基因组总DNA进行提取,并对DNA进行质量检测。结果表明,改良CTAB法可从天门冬成熟叶片中获得纯度高、完整性好的总DNA,其OD260 nm/OD280 nm为1.86,OD260 nm/OD230 nm为2.36,浓度为390μg/mL,产率达11.7μg/g,多糖、多酚和RNA杂质被去除干净,无降解现象,说明该方法提取的天门冬基因组总DNA质量较高。  相似文献