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Tadao Mitui Hiroshi Onaga Yoji Nagasawa Yoshitoshi Nomura Shigemi Kuramasu 《Veterinary microbiology》1981,6(4):339-349
A latex agglutination test was evaluated as a method for the detection and titration of antibodies against swine Haemophilus infection and it was found that the test is applicable to the etiologic diagnosis of Haemophilus infection in swine. In swine infected experimentally with H. pleuropneumoniae (H. parahaemolyticus), the micromethod of agglutination using latex particles coated with antigens of H. pleuropneumoniae was found to be comparable agar-gel immunodiffusion and complement-fixation tests, which have previously been used for the etiologic diagnosis of the disease. Antibody titers determined by the latex agglutination test corrlated well with those determined by the other serological tests. The latex agglutination test tended to detect antibodies earlier than any of the other tests. By the latex agglutination test, weak cross-reactions were observed among different serotypes of H. pleuropneumoniae, whereas no cross-reaction was demonstrated between H, pleurophneumoniae and H. parasuis. The latex agglutination test was found to be simple and useful for the serological survey of swine Haemophilus infection, especially when dealing with a large number of samples. 相似文献
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Agar blocks colonized by Sclerotinia sclerotiorum, causal agent of lettuce drop, were used as inoculum in screening for resistance to this pathogen in the greenhouse.Since the degree of resistance to the pathogen among cultivars varied with the time observed after inoculation, resistance was assessed by the number of days for 50% of the plants of each cultivar to die, as well as the number of plants that died. The cultivars ‘PI (Plant Introduction) 250427’, ‘Taiwan’, ‘PI 251790’, ‘PI 255568’, ‘MSU 73-44’ and ‘Bibb’ were found to have a higher level of resistance to S. sclerotiorum than the cultivar ‘Grand Rapids’ at 12 or 15 days following inoculation. 相似文献
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Morris D. Schneider Yutaka Furusho Shigemi Iiyama 《Canadian journal of veterinary research》1965,29(4):97-101
Three hyperimmune dogs were injected intramuscularly with canine wild rabies virus. The dogs showed no clinical evidence of rabies. Intramuscular injection of the wild rabies virus in susceptible puppies induced fulminating disease and mortality in 14 to 16 days. Investigation of the serum proteins of the hyperimmune dogs by a paper electrophoresis technique revealed conspicuous changes in the beta and gamma protein bands. 相似文献
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The fate of cystic ovarian follicles that developed spontaneously during the early postpartum period of 50 lactating dairy cows was traced by ultrasonography to characterise the follicular dynamics in relation to the fertility of the cows. The absence of postpartum ovulations caused by repeated waves of anovulatory large follicles was also characterised and evaluated. Fifteen of the 50 cows developed cystic follicles, and these follicles became follicular cysts in five of the 15 cows. Most of the cystic follicles emerged before the first postpartum ovulation of the cows. The transition from cystic follicles to follicular cysts delayed the cows' first ovulation, oestrus and insemination, but had less influence on their fertility after they had recovered spontaneously. In addition to the 15 cows that developed cystic follicles or follicular cysts, six of the cows had five to 13 waves of follicles before their first ovulation. These repeated waves of follicles caused a more severe delay in the early postpartum reproductive events but did not affect the cows' fertility. 相似文献
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Use of carefully lifted, decapitated cauliflower plants with 6 to 7 leaves per se, eliminated the need of the rooting of shoots and provided seeds in reduced time. Previously, shoots obtained from decapitated and de-leafed plants were rooted and allowed to produce seeds. This latter method did not assure that seed would be available in time for the following season's planting. 相似文献
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Hamako Sasamoto Yohichi Wakita Shinso Yokota Nobuo Yoshizawa 《Journal of Forest Research》2000,5(4):265-270
An improved method for selection of large electro-fused protoplasts ofPopulus alba by a micromanipulator was developed. The conditions for electric cell fusion treatment were optimized. For the best result,
protoplasts with a cell density of 5 × 105/mL were treated with an alternate current (1 MHz, 200 V/cm) and pulsed with a direct current (2 kV/cm) for 100μs in 2.5 mM CaCl2 and 0.55 M mannitol. The electo-fused protoplasts were cultured in NH4NO3-free Murashige and Skoog’s medium containing 0.6 M of mannitol, 0.09 M sucrose, 1μM of 2,4-dichlorophenoxyacetic acid and 0.1μM of benzyladenine, the same medium used for protoplast culture, but at a very low cell density of 5–10 × 102/mL in a well of a 96-well culture plate. When cell aggregates derived from individual fused protoplasts were transferred
to fresh medium with 0, 0.3 or 0.6 M mannitol, large colonies developed. In the shoot differentiation medium, the reaction
of the calluses derived from large fused protoplasts towards the growth regulators differed from the non-fused ones. In medium
containing 1μM each of naphthalene acetic acid andN-(2-chloro-4-pyridyl)-N′-phenylurea, growth of callus from electro-fused ones was not reduced by much compared to the control, but shoot differentiation
was inhibited. Gibberellic acid (0.1–10μM) was beneficial to shoot regeneration; however, irregularly shaped leaves appeared at high gibberellic acid concentrations.
Shoots regenerated were rooted in Murashige and Skoog’s medium containing 4μM of indole-3-butyric acid. Some plantlets obtained had a varied morphology. Based on the characteristics of growth, some
cells derived from electro-fused protoplasts appear to be physiologically different from the non-fused one. 相似文献
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Kurosaki N Yamato O Sasamoto Y Mori F Imoto S Kojima T Yamasaki M Maede Y 《The Japanese journal of veterinary research》2007,55(1):3-12
In our previous study, it was demonstrated that the administration of anion salts, which slightly lower the dietary cation-anion difference (DCAD), in the prepartum period is safe and effective for preventing milk fever in multiparous cows. In the present study, several clinico-pathological constituents in serum and urine, which might be related to milk fever, were analyzed using stored samples from the previous study to identify clinico-pathological parameters for easily evaluating the efficacy of lowering DCAD and to further investigate the mechanism by which lowering DCAD prevents milk fever. Among the parameters analyzed in the present study, inorganic phosphorus (iP) was involved in milk fever because the serum concentration and urinary excretion of iP were significantly higher in the group of primiparous cows (heifer group), which did not develop hypocalcemia, than those in other groups of multiparous cows. Serum chloride concentrations in the heifer group and the group of multiparous cows fed anion salts (anion group) tended to remain higher than those in other control groups of multiparous cows suggesting that serum chloride concentration may be utilized for evaluating the status of metabolic acidosis and the efficacy of lowerng DCAD in dairy cows fed anion salts. In addition, plasma estradiol-17beta concentration in the heifer group tended to be lower at parturition compared with that in other multiparous groups suggesting that estrogen known as a potent inhibitor of bone resorption may be involved in developing milk fever. 相似文献