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1.
龙牙百合的植株再生与遗传转化 总被引：19，自引：0，他引：19
以龙牙百合鳞片叶切块为外植体，通过多种培养基的比较试验，得出MS 2，4-D 2mg／L 6-BA 0．2mg／L是诱导愈伤的最佳培养基，MS 6-BA 1mg／L NAA 0．05mg／L是不定芽诱导及伸长的适宜培养基。MS N_AA2mg／L是生根的最佳培养基。本试验已建立了适用于龙牙百合遗传转化的快速高频再生系统。用携带有几丁质酶基因和β—1、3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经PCR和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16．7％，基因枪法的转化率为50％，因此可能基因枪转化法更适于龙牙百合的遗传转化。 相似文献
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Yaohu?KangEmail author Feng-Xin?Wang Hai-Jun?Liu Bao-Zhong?Yuan 《Irrigation Science》2004,23(3):133-143
A field experiment comparing different irrigation frequencies and soil matric potential thresholds on potato evapotranspiration (ET), yield (Y) and water-use efficiency (WUE) was carried out in a loam soil. The experiment included five treatments for soil matric potential: F1 (-15 kPa), F2 (-25 kPa), F3 (-35 kPa), F4 (-45 kPa) and F5 (-55 kPa) and six treatments for irrigation frequency: N1 (once every day), N2 (once every 2 days), N3 (once every 3 days), N4 (once every 4 days), N6 (once every 6 days) and N8 (once every 8 days). Results indicate that both soil matric potential and drip irrigation frequency influenced potato ET, Y and WUE. Potato ET increased as irrigation frequency and soil matric potential increased. Comparing soil water potential, the highest ET was 63.4 mm (32.1%) more than the lowest value. Based on irrigation frequency treatments, the highest ET was 36.7 mm (19.2%) more than the lowest value. Potato Y and WUE were also found to increase as irrigation frequency increased. Potato Y increased with an increase in soil water potential then started to decrease. The highest Y and WUE values were achieved with a soil matric potential threshold of -25 kPa and an irrigation frequency of once a day.Communicated by J. Ayars 相似文献
3.
Shanshan?Chen Jiahuan?Tang Li?Fu Yong?Yuan Shungui?ZhouEmail author 《Journal of Soils and Sediments》2016,16(9):2326-2334
Purpose
The low conductivity of sediments for mass and electron transport is the most severe limiting factor in sediment microbial fuel cells (SMFCs), so that sediment ameliorations yielded more remarkable effects than electrode improvements. The objective of this research was to enhance the electricity generation of SMFCs with amendments of biochar to freshwater sediments for conductivity enhancement.Materials and methods
Laboratory-scale SMFCs were constructed and biochars were produced from coconut shells at different temperatures. Variations in the power output, electrode potential, internal resistance, total organic carbon (TOC) content, and microbial communities were measured.Results and discussion
Amending with biochar reduced the charge transfer resistances of SMFCs and enriched the Firmicutes (mainly Fusibacter sp.) in the sediment, which improved the SMFC power generation by two- to tenfold and enhanced the TOC removal rate by 1.7- to fourfold relative to those without the amendment.Conclusions
The results suggested that biochar amendment is a promising strategy to enhance SMFC power production, and the electrical conductivity of biochar should be considered important when interpreting the impact biochar has on the electrical performance of soil or freshwater sediment MFCs.4.
本文以组织培养2～13周的丹参幼苗为材料，构建丹参cDNA文库并进行大规模EST序列分析，所得序列经NCBI的BLAST工具分析，克隆号为rsmsxl_014903．y1．scf序列与晚期胚胎丰富(late embryogenesis abundant)基因家族Ⅱ中的成员有较高的同源性。该序列全长864bp，包含1个长726bp开放阅读框(ORF)，编码241个氨基酸，并含有ⅡLEA蛋白的特征序列，与NCBI注册的脱水素家族基因具有较高的同源性，表明本基因可能是一种新的脱水素基因，命名为DHN2，并在GenBank上进行了注册，序列号为：AY737725。 相似文献
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6.
水稻细菌性条斑病及其抗性研究进展 总被引：13，自引：0，他引：13
本文简要综述了近年来国内外对水稻抗细菌性条斑病在病原菌鉴定、水稻抗源筛选、抗性生理遗传以及抗性的分子生物学等方面的研究进展，以期为水稻细条病的深入研究提供参考。 相似文献
7.
结核免疫与结核新疫苗的研究进展 总被引：1，自引：0，他引：1
本文对近十多年来有关结核免疫的生物学机制和结核疫苗研究进展进行了综述，指出结核疫苗的发展方向，尤其指出植物疫苗是最为廉价、有前途的发展之路。 相似文献
8.
Filipe?Selau?CarlosEmail author Bruno?Loss?dos?Santos Robson?Andreazza Marino?José?Tedesco Lawrence?Morris Flávio?Anastácio?de?Oliveira Camargo 《Paddy and Water Environment》2017,15(1):133-144
Treated industrial effluents have high levels of nutrients and dissolved organic matter. The irrigation of rice by flooding can increase nutrient uptake and grain yield. Therefore, this study evaluated the nutrient contents in the shoots and grain of the rice crop and also the chemical of the soil after irrigation of the crop with leachate of the treated industrial effluent. A greenhouse experiment was conducted using pots filled with 20 kg of soil in a randomized block design with three replications. The treatments consisted of control (irrigation with distilled water) and four concentrations of the leachate (25, 50, 75, and 100 %) for irrigation. At the end of the experiment, the nutrient contents in tissues of rice plants, sterility of spikelets, and grain mass were evaluated. Results showed that irrigation with the leachate at 25 % content increased the macro- and micronutrients’ concentrations in the shoot biomass and grain, except for potassium and iron. Irrigation with the industrial leachate decreased tillering and grain yield; however, it increased chlorophyll content, sterility of spikelets, and sodium intake at this leachate concentration. The potassium and sodium levels and the electrical conductivity values of soils irrigated with treated industrial leachate were increased. The use of the treated leachate from industrial effluents is an alternative that reuses the nutritional load, but the volume of leachate should be limited and monitored to prevent the sodicity in the soil and problemsdue to eutrophication. 相似文献
9.
Mathias?FosuEmail author Ronald?F.?Kühne Paul?L.?G.?Vlek 《Biology and Fertility of Soils》2003,39(2):117-122
In order to understand the efficiency of residue-N use and to estimate the minimum input required to obtain a reasonable level of crop response, it is important to quantify the fate of the applied organic-N. The recovery of N from 15N-labelled Crotalaria juncea was followed in the soil and the succeeding maize crop. Apparent N recovery (ANR) by maize from unlabelled Crotalaria juncea, Crotalaria retusa, Calopogonium mucunoides, Mucuna pruriens and mineral fertilizer at three locations were also evaluated. The maize crop recovered 4.7% and 7.3% of the 15N-labelled C. juncea-N at 42 days after sowing (DAS) and at final harvest, respectively. The corresponding 15N recovery from the soil was 92.4% and 58.5%. The highest mean ANR of 57.4% was with mineral fertilizer, whereas the mean ANR of 14.3% from C. retusa was the lowest. A large pool substitution and added-N interaction effect was observed when comparing N recovery from the labelled and unlabelled C. juncea. The amount of residue-N accounted for by the isotope dilution method at 42 DAS was 97.1% and at final harvest 65.8%. The large residue-N recovery in the soil organic-N pool explains the residual effect usually observed with organic residue application. 相似文献
10.
M.?AshrafEmail author T.?Mahmood F.?Azam R.?M.?Qureshi 《Biology and Fertility of Soils》2004,40(2):147-152
The beneficial role of green manures in rice production is generally ascribed to their potential of supplying plant nutrients, particularly nitrogen (N). However, the mechanisms through which green manures enhance the crop productivity are poorly understood. Pot experiments were conducted using a 15N-tracer technique: (1) to compare the biomass production potential of sesbania (Sesbania aculeata Pers.) and maize (Zea mays L.) as green manuring crops for lowland rice and (2) to compare the effect of the two types of green manure and inorganic N on the dry matter accumulation and N uptake by two rice (Oryza sativa L.) cultivars, viz. IR-6 and Bas-370. Although maize produced three times higher shoot biomass compared with sesbania, the latter showed higher N concentration; and thus the total N yield was similar in the two types of plants. Applying the shoot material of the two plants to flooded rice significantly enhanced the dry matter yield and N uptake by the two rice cultivars, the positive effects generally being more pronounced with sesbania than with maize amendment. The difference in the growth-promoting potential of the two plant residues was related more to an increased uptake of the native soil N rather than to their direct role as a source of plant-available N. A positive added nitrogen interaction (ANI) was observed due to both plant residues, the effect was much more pronounced with the application of sesbania than with maize residues. In both rice cultivars, inorganic N also caused a substantial ANI, particularly at higher application rate. Losses from the applied N were 2–3 times lower from sesbania, compared with maize treatment. Green manuring with sesbania also caused much lower N losses than the inorganic N applied at equivalent or higher rates. The overall benefit of green manuring to rice plants was higher than inorganic N applied at comparable rates. The two rice cultivars differed in their response to green manuring, IR-6 generally being more responsive than Bas-370. 相似文献