Different biological behaviour of Waldenström macroglobulinemia in two dogs     

F. Gentilini  C. Calzolari  A. Buonacucina  M. Di Tommaso  G. Militerno  P. Famigli Bergamini 《Veterinary and comparative oncology》2005,3(2):87-97

Waldenström Macroglobulinemia is a low‐grade immunosecretory disorder associated with lymphoid tumours, which is rarely reported in veterinary medicine. In this study, we describe two clinical cases of this rare syndrome in dogs, each characterized by a different onset and clinical course. In one case, a hyperacute onset and aggressive behaviour of the neoplasm was observed. Absolute serum viscosity (SV) was retrospectively evaluated in order to explain clinical findings. Rotational viscosimetry showed good precision in measuring SV. Both dogs had SV values higher than a control groups of healthy dogs although only one subject developed hyperviscosity symptoms and complications. At high paraprotein concentrations, a slight reduction of the M‐component was associated with a marked decrease in SV. Thus, this work suggests that SV assessment is a relevant tool for managing monoclonal gammopathies, whose usefulness should be further confirmed in larger cohorts of dogs.  相似文献   

Validation of a human immunoturbidimetric assay to measure canine albumin in urine and cerebrospinal fluid.     

Fabio Gentilini  Francesco Dondi  Cinzia Mastrorilli  Massimo Giunti  Claudia Calzolari  Gualtiero Gandini  Danilo Mancini  Paolo Famigli Bergamini 《Journal of veterinary diagnostic investigation》2005,17(2):179-183

The aim of this study was to validate an automated immunoturbidimetric assay used to quantify human albumin in urine and to accurately measure canine albumin concentrations in both urine and cerebrospinal fluid. The partial homology existing between human and canine albumin limited the accuracy of the human assays in measuring canine albumin without method modifications. Thus, the assay was modified by calibrating the analyzer with calibrators made in the laboratory containing known concentrations of canine albumin. To prepare the set of calibrators, the albumin concentration of pooled sera of healthy dogs was assessed in 5 replicates using the BromocresolGreen assay. Pooled samples were aliquoted and serially diluted to obtain the expected concentrations of albumin (0.5, 1, 5, 13, and 30 mg/dl) for establishing the canine calibration curve. Thereafter, the performance was assessed by analyzing canine urine and CSF The modified assay accurately quantified canine albumin in both specimens, as indicated by the following. Intra- and interassay variability was 0.92% and 2.74%, respectively; recovery was 99.66% and 99.07% in urine and 105.02% in CSF No interference was detected when hemolysate and glucose were added to urine. The test was linear within the verified range (0-225 mg/dl). These results demonstrate that the modified human albumin immunoturbidimetric assay can be a useful tool in the veterinary diagnostic laboratory. It is accurate and tends itself to automatization on chemistry analyzers.  相似文献   

Effect of dips in a 1-methylcyclopropene-generating solution on 'Harrow Sun' plums stored under different temperature regimes     

Manganaris GA  Vicente AR  Crisosto CH  Labavitch JM 《Journal of agricultural and food chemistry》2007,55(17):7015-7020

The effect of postharvest dips in a 1-methylcyclopropene-generating solution of the formulation AFxRD-038 (Rohm & Haas) on plum fruit (Prunus salicina Lindell cv. 'Harrow Sun') quality and ripening during storage was determined. Fruit weight loss, tissue firmness, soluble solids content (SSC), titratable acidity (TA), ethylene production, respiration, and the activities of the cell wall modifying enzymes polygalacturonase (PG), 1,4-beta-D-glucanase/glucosidase (EGase), beta-galactosidase (beta-gal), and pectin methylesterase (PME) were measured. Fruit reddening, anthocyanin content, and phenylalanine ammonia-lyase (PAL) activity were also analyzed. The 1-MCP-treated fruit showed reduced ethylene production and respiration rate and delayed softening, which was associated with the reduction in the activity of PG, EGase, and beta-gal. The immersion in 1-MCP-generating solutions also decreased weight and acidity loss without modifying the fruit SSC. The immersion treatment was particularly effective in the fruit stored at 5 degrees C, keeping higher overall quality, maintaining lower levels of anthocyanins and PAL activity, and preventing flesh reddening. The present data show that beneficial effects in delaying plum fruit ripening and controlling chilling injury can be obtained by dipping the fruits in a solution of this novel 1-MCP-generating formulation.  相似文献   

A Modeling Study of the Impact of a Power Plant on Ground-Level Ozone in Relation to its Location: Southwestern Spain as a Case Study     

Nuria Castell  Enrique Mantilla  Ariel F. Stein  Rosa Salvador  Millán Millán 《Water, air, and soil pollution》2010,209(1-4):61-79

The impact of atmospheric industrial emissions on secondary pollutant formation depends on many factors; one of the most important being the environmental setting in which the industry is located. The environmental setting affects an industry’s impact on ozone levels through both the air mass dispersion (a function of topography and wind patterns) and the emissions of organic volatile compounds (VOC) and nitrogen oxides (NO _x ) in the area. This model-based study shows how the sensitivity of surface ozone changes with the choice of source location. For the analysis, seven points distributed along the Tinto–Guadalquivir Basin (in the Southwestern Iberian Peninsula) were selected. This area is characterized by the close proximity of natural environments and crop fields to cities, roads, and industrial areas with high NO _x emissions. Natural VOC emissions represent more than 60% of the total non-methane volatile organic compounds emitted in the study area. The results reveal that the largest increases in ozone levels are produced when the industry is located both far away from NO _x emission sources and near to biogenic VOC emissions. Furthermore, the highest increases over the hourly and 8-hourly maximums, as well as the highest accumulated daily values, are found in areas characterized by high VOC/NO _x emission ratios and NO _x sensitivity. The study of the recurrent meteorological patterns along with the distribution of chemical indicators of the O₃–NO _x –VOC sensitivity allows the determination of the industry’s geographical impact on ozone levels. This information enables air quality managers to decide the future location of an industry minimizing its impact on smog levels.  相似文献   

Geographical distribution of the DL1 and DL2 genes causing hybrid dwarfism in Phaseolus vulgaris L., their association with seed size,and their significance to breeding   总被引：1，自引：0，他引：1  

Shree P. Singh  J. Ariel Gutiérrez 《Euphytica》1984,33(2):337-345

Summary Dwarlism in F₁ hybrids has been observed in over 100 crosses of dry beans (Phaseolus vulgaris L.) at the Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia. In each cross, one parent always had small seeds and the other parent either medium or la ge ones. This apparent incompatibility between the two groups of germplasm was controlled by two complementary, dominant genes: DL₁ and DL₂. Smallseeded bean lines carried gene DL₁ and originated in Brazil, Colombia, Guatemala, and Mexico; medium for large-seeded bean lines carried gene DL₂ and were from Bolivia, Brazil, Chile, Colombia, Turkey, The United States, and West Germany. Thes two genes have probably played an important role in the evolution of dry bean forms of different seed sizes by serving as a genetic barrier or isolating mechanism, thus limiting free genetic recombination between the two germplasm groups.Apparent differences in the adaptiveness and yielding ability of the two groups of bean germplasm, smallys, medium- and large-seeded, and some breeding implications for manipulation of the genes causing F₁ hybrid dwarfism were also discovered.  相似文献   

Effect of plant density on selection for seed yield in two population types of Phaseolus vulgaris L.     

Shree P. Singh  J. Ariel Gutiérrez 《Euphytica》1990,51(2):173-178

Summary Pedigree selection for seed yield, using early generation yield tests, was practiced from the F₂ to F₇ in two populations of common bean (Phaseolus vulgaris L.) in three plant densities: 66, 133, and 266 thousand plants ha^-1 at CIAT-Palmira, Colombia. The six highest yielding lines selected from each plant-density, along with 13 parents, were evaluated in a 7×7 lattice design with three replications at the three densities utilized for selection, and at 399,000 plants ha^-1 in 1988 and 1989.Based on the mean performance of selected lines and the mean of the parents, selection for seed yield was effective in all densities in both populations. However, none of the lines selected from the population within the race Mesoamerica (TC 4673) significantly outyielded their best parent under any plant density. The highest yielding lines selected from the interracial population (TR 4635) outyielded their best parent irrespective of the plant-density used for selection. The highest yielding line originated from the highest density used for selection. Low density was neither good for selection nor for evaluation and identification of high-yielding cultivars of common bean. There was no significant difference between the mean yield of lines selected at the intermediate and high population densities. The effects of plant density, year, and their interactions were significant for seed yield.  相似文献   

The search for carbon sinks in the tropics     

Ariel E. Lugo 《Water, air, and soil pollution》1992,64(1-2):3-9

The study and modeling of the global C cycle have been dominated by the assumption that the atmosphere and the biota were in C steady state prior to the industrial revolution. This view led to the perception that most of the terrestrial biota was neutral with regards to the C concentration of the atmosphere. Recent evidence suggests that neither the atmosphere nor the biota were in C steady state prior to, or since, the industrial revolution. Therefore, it is now necessary to re-visit the role of natural processes in the global C cycle, study the C cycle in its totality, and focus attention on the magnitude of potential C sinks in ecosystems previously thought to be neutral with respect to atmospheric C. “An improved understanding of the CO₂ cycle is essential to predict the future rate of any atmospheric CO₂ increase and to plan eventually for an international CO₂ management strategy” Tans et al. (1990).  相似文献   

Mineral nutrient fluxes in rainfall and throughfall in a lowland Atlantic rainforest in southern Brazil     

Maurício Bergamini Scheer 《Journal of Forest Research》2011,16(1):76-81

The ecosystems occurring on dystrophic soils, such as sandy soils, are highly dependent on nutrients from the atmosphere and those cycled by their own biota. Nutrient inputs from rainfall and throughfall were measured between October 2001 and August 2003 in a secondary Atlantic rainforest in southern Brazil. Canopy interception (rainfall minus throughfall) was 17.3% of the annual rainfall of 2,235 mm. Monthly interception ranged from 12 to 31% during the rainiest months (precipitation above 200 mm) and from 1 to 45% during the driest months (precipitation below 50 mm) indicating relatively high variability during this period. The studied site may be susceptible to water stress in this period due to the high permeability of the sandy soil. Approximately 80% of the Ca and Na and 57% of Mg were mainly from rainfall (bulk deposition) whereas the main input source for K was net throughfall (about 78%). Mean annual inputs via throughfall (in kg ha⁻¹) were: 90.6 for Na, 29.1 for K, 7.1 for Ca, and 2.9 for Mg. The highest nutrient inputs occurred during the rainy season. Na fluxes were relatively high, while K, Ca, and Mg inputs were low, compared with other tropical and subtropical forests. Information on nutrient fluxes for different forest ecosystems are fundamental for building up a database that can give support to environmental diagnosis, to forest management, and to conservation and restoration techniques.  相似文献   

Acknowledgments     

Dr. Joe Wisniewski  Dr. Ariel E. Lugo 《Water, air, and soil pollution》1992,64(1-2):xi-xi

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Generation of spatially coherent light at extreme ultraviolet wavelengths     

Bartels RA  Paul A  Green H  Kapteyn HC  Murnane MM  Backus S  Christov IP  Liu Y  Attwood D  Jacobsen C 《Science (New York, N.Y.)》2002,297(5580):376-378

We present spatial coherence measurements of extreme ultraviolet (EUV) light generated through the process of high-harmonic up-conversion of a femtosecond laser. With a phase-matched hollow-fiber geometry, the generated beam was found to exhibit essentially full spatial coherence. The coherence of this laser-like EUV source was shown by recording Gabor holograms of small objects. This work demonstrates the capability to perform EUV holography with a tabletop experimental setup. Such an EUV source, with low divergence and high spatial coherence, can be used for experiments involving high-precision metrology, inspection of optical components for EUV lithography, and microscopy and holography with nanometer resolution. Furthermore, the short time duration of the EUV radiation (a few femtoseconds) will enable EUV microscopy and holography to be performed with ultrahigh time resolution.  相似文献