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1.
An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato 总被引：2，自引：0，他引：2
Andrzej Pałucha Włodzimierz Zagórski Mirosława Chrzanowska Danuta Hulanicka 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(3):287-293
The Bzura commercial potato cultivar was transformed by sense or antisense constructs which included the coat protein gene of potato leafroll virus RNA. In the sense construct, the coat protein gene was preceded by a leader sequence shorter than that in the subgenomic RNA formed in infected cells. The antisense construct consisted of a sequence complementary to the first 2020 nucleotides of the subgenomic RNA. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. In one line, expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants and therefore this transformant might be regarded as virus immune. 相似文献
2.
The dimethylformamide extracts from sewage sludges from a chosen sewage — treatment plant in highly industrialized region of Poland were investigated. Using the column chromatography with Al2O3 and silicic acid as the stationary phases, the fraction of azaarenes has been separated. GC-MS analysis of azaarenes fraction made it possible to identify 10 azaarenes and several of their alkyl derivatives, which dominate in the organic matter extracted from sewage sludges. The silicic acid column chromatography in connection with the reduction of nitroarenes and derivatization with pentafluoropropionic acid anhydride yielded an enriched nitrofraction. Nitroarenes present in this fraction as amide derivatives were also analysed by GGMS method. There was found presence of 3 nitroarenes and their methyl derivatives. 相似文献
3.
Loska Krzysztof Cebula Jan Pelczar Jacek Wiechuła Danuta Kwapuliński Jerzy 《Water, air, and soil pollution》1997,93(1-4):347-365
In this paper, the contamination degree of the Rybnik Reservoir with cadmium, copper and nickel was analyzed. Quality of the water from the reservoir was determined by drawing comparisons between the metal content in the water and both the officially permitted levels (contamination factor) and levels of metals occurring in the water of non-contaminated areas (enrichment factor). Contamination of bottom sediment with chosen metals was analyzed with reference to the metal content in mudstone (geoaccumulation index, enrichment factor, contamination factor). Trends towards changing the metal content in the bottom sediment was analyzed by determining the enrichment factor of the surface layer of the bottom sediments in relation to a deeper layer. Enrichment of the bottom sediments with metals coming from the water was also determined. 相似文献
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Forms of cadmium occurrence in the bottom sediment of a heated water reservoir, polluted substantially with this metal was analysed in the paper. Apart from the analysis mentioned above, a total cadmium content in the bottom sediment and a content of cadmium dissolved in the water of the reservoir was also determined. Dependences between particular forms of cadmium occurrence and its total content in the bottom sediment were analysed. An influence of cadmium content in water on the level of particular cadmium forms in the bottom sediment and an influence of other parameters of the reservoir's water on the content of cadmium in the bottom sediment were also examined. 相似文献
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J. Wegner Ilse Fiedler Danuta Klosowska B. Klosowski Brigitte Ziegan 《Anatomia, histologia, embryologia》1993,22(4):355-359
Changes in the distribution of muscle-fiber-types from the M. longissimus dorsi of male pigs. A Histochemical Approach Samples of the M. longissimus dorsi from growing up pigs were taken by a shooting-biopsy. The types of muscle-fibers could be demonstrated by two different histochemical staining procedures. With these two histochemical staining procedures significant differences between the different structure of the muscle-fibers during increasing age of the pigs could be detected. 相似文献
7.
Bodzek Danuta Luks-Betlej Krystyna Janoszka Beata 《Water, air, and soil pollution》1998,103(1-4):91-100
The Polycyclic Aromatic Hydrocarbons (PAHs) pollution state of the chosen town (Zabrze) of Upper Silesia, the most industrialised region in Poland, was presented. Zabrze is a town of medium size, having population twenty times bigger than a medium town polulation in Poland. In this study we determined the qualitative and quantitative composition of PAHs fraction separated from the following elements of environment: suspended dust, drinking water, soil and sewage sludge. The following analytical techniques were used: Soxhlet Extraction, Solid Phase Extraction, Gas Chromatography-Mass Spectrometry, Capillary Gas Chromatography and High Performance Liquid Chromatography. The results confirmed the significant state of examined region pollution. They stated the exceed of contents B(a)P in suspended dust. The soils in that place were heavily polluted with PAHs, about 4000 µg · kg-1 for the sum of PAHs. The total contents of PAHs in sewage sludge of Zabrze did not exceed the analogous contents published for West European sewage sludge treatment plants and the level of determined PAHs in drinking water did not show any claims. 相似文献
8.
The total contents of PAHs, organic polar fractions and 14 major hydrocarbons containing 3–6 aromatic rings as well as heavy metal contents were determined in sewage sludges from highly industrialized (Upper Silesia), agricultural and recreational regions of Poland. Sludges from the industrial region showed markedly increased concentrations of all the organic micropollutants and 2–10 times higher levels of the following heavy metals: Pb, Cd, Cr, Cu, Fe, Zn, Ni, Mn and Co. The levels, however, did not exceed the Polish as well as European Economic Community limit values, which renders all the sludges applicable to land and some of them suitable for utilization on agricultural soils. 相似文献
9.
Krzysztof Dziedzic Danuta Górecka Artur Szwengiel Hanna Sulewska Ivan Kreft Elżbieta Gujska Jarosław Walkowiak 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(1):82-88
In this report, we presented the profile of polyphenolic substances in flowers, leaves, stalk and roots of Fagopyrum tataricum estimated by using RP–UHPLC–ESI-MS equipment (reversed-phase ultra-high-performance liquid chromatography electrospray ionisation mass spectrometry). The neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose and hemicellulose were also determined. Flowers, leaves, stalk and roots showed varying levels of dietary fibre and polyphenols. The highest content of neutral and acid detergent fibre were found in the roots (63.92 and 45.45% d.m., respectively) while the most rich in phenolic compounds were flowers (4.8 mg/1 g d.m.). Root and stalk contained the highest level of cellulose, 38.70 and 25.57% d.m., respectively. Among the investigated polyphenolic substances such as: 2,6-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydrobenzoic acid, caffeic acid, catechin, chlorogenic acid, fagopyrin, ferulic acid, myricetin, gallic acid, isovanilic acid, isovitexin, kaempferol, luteolin, p-coumaric acid, procyanidin B2, quercetin, quercetin 3-D galactoside, rutin, syringic acid and vitexin, we observed that the contents of rutin and chlorogenic acid were the highest. We found some correlation between dietary fibre fractions and individual phenolic substances. The levels of acid detergent fibre (ADF), cellulose and hemicellulose were negatively correlated with isovitexin, kaempferol, vitexin, fagopyrin, caffeic acid and procyanidin B2 content. In this investigation, two solvents (water and methanol) were estimated regarding their extraction efficiency of phenolic compounds. Taking these results into consideration, we recommend using methanol as the extractor to isolate chlorogenic acid, fagopyrin, kaempferol, procyanidin B2, quercetin, quercetin 3-D-galactoside, rutin, vitexin, and water for other investigated polyphenolic substances obtained from Fagopyrum tataricum. 相似文献
10.
Delaney Kevin J Breza-Boruta Barbara Lemańczyk Grzegorz Bocianowski Jan Wrzesińska Danuta Kalka Ireneusz Piesik Dariusz 《植物病害和植物保护杂志》2015,122(3):125-132
Journal of Plant Diseases and Protection - Plants induce volatile organic compounds (VOCs) after pathogen infection and exposure to a neighbouring infected plant. In a greenhouse, we measured VOCs... 相似文献