Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene          下载免费PDF全文

Yang Zhao  Yuedong Xuan  Daolin Mou  Hong Liu  Pan Zhou  Zhengfeng Fang  Lianqiang Che  Shengyu Xu  Bin Feng  Jian Li  Xuemei Jiang  Yan Lin  De Wu 《Journal of animal physiology and animal nutrition》2018,102(5):1340-1350

The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB₁₂, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor.  相似文献   

发酵酸羊乳冻干粉的生产方法     

李志才  牟善波  姜竹茂  王利义  徐少同 《中国乳业》2021,(1)

介绍了发酵酸羊乳冻干粉的生产方法,主要有羊乳预处理、羊乳发酵、冻干制粉和包装质检四个生产工序。应用此法生产的酸羊乳冻干粉保留了酸羊乳原有的色、香、味和营养物质,还具有较高的乳酸菌生物活性。  相似文献   

Design method and homogeneity control of bridge fair-faced concrete     

Zhou Xiaojun  Mou Tingmin  Ding Qingjun  Nie Chuanzhen 《保鲜与加工》2015,(1):117-122

According to the structural characteristics of bridge engineering, the mix optimization design method of bridge fair-faced concrete was presented, and C30~C50 concrete with high cohesion and package, outstanding fluidity and excellent mechanical properties were prepared. Through the test of air content of fresh concrete, micro hardness and micro structure in different parts of hardened concrete specimens, effects of viscosity-modifying admixtures on homogeneity of concrete were discussed. The results showed that the composition of bridge fair-faced concrete were relatively homogeneous, and the microstructure were relatively dense. When C30 concrete mixed with 5 × 10 -5 cellulose ether or 6% silica fume (mass fraction of cementitious material), air content of fresh concrete were less than 2%, and slurry viscosity values were between 1 900 and 2 000 MPa·s. Also, the micro hardness values in different parts of hardened concrete specimens were closed. The results were applied in practical bridge engineering, which generally meets the apparent quality requirements of bridge fair faced concrete.  相似文献   

早籼型水稻温敏核不育系M103S的选育与光温反应特性   总被引：3，自引：2，他引：3  

牟同敏  李春海 《华中农业大学学报》2003,22(3):213-218

以温敏核不育水稻安农S—1为母本，以常规高产早籼稻品系92153为父本进行杂交，通过低温选择和人工气候箱鉴定，育成了育性转换临界温度23—24℃的早籼型温敏核不育系M103S。光周期长于12．5h，日平均温度高于24℃，可以用于制种，日平均温度低于23℃，可以用于繁殖。光周期长于13．5h，日平均温度高于23℃，可以用于制种。自然条件下不育起点温度为日均温23—24℃，耐低温期为3—4d。敏感期为抽穗前的7—14d。播始历期为53—79d，主茎叶片数10．0—12．6片。从播种到始穗的活动积温和有效积温，分别为1493．8—1766．3℃和733．4—1058．3℃。不育期的株高63．6—77．5cm，平均72．0cm，单株有效穗6．4—12．0个，平均9．3个，穗长20．9—22．7cm，平均21．2cm，每穗颖花数112．1—137．3朵，平均122．3朵。可育期株高65．7cm，单株有效穗7．2个，穗长17．2cm，每穗总粒数114．1粒，结实率47．23％，千粒重31．34g。以M103S为母本，与100多个早籼品种进行测配，杂种表现生育期早、穗大、粒多、千粒重高。  相似文献   

牡丹江地区欧洲白桦生长性状变异分析   总被引：1，自引：1，他引：0  

于长玲  兰士波  刘俊国 《中国林副特产》2009,(2):9-11

在牡丹江林区欧洲白桦各家系均能安全越冬,苗木长势良好,且区组间欧洲白桦家系苗木的高生长变异较小,苗高性状差异不显著,苗木地径生长差异显著。2007年和2008年苗高变异幅度为16.20%-28.18%、17.86%-23.70%,平均变异系数分别为23.20%和20.57%;区组间欧洲白桦家系苗木的地径生长变异较小,2007年和2008年苗高变异幅度分别在16.94%-21.89%、22.63%-33.74%范围内,平均变异系数分别为21.28%和32.04%。  相似文献   

贵州省退耕还林工程社会经济效益阶段评价研究   总被引：2，自引：0，他引：2  

周红周军  张晓珊缪杰 《贵州林业科技》2007,35(2):1-6

通过对水城、黎平、望谟、关岭、麻江、黔西、龙里、习水、开阳、铜仁10个退耕还林工程综合效益监测重点县107个固定样地户主共91户退耕农户进行连续4年的跟踪调查，同时结合10个县面上统计数的分析及其典型县204户退耕农户的典型调查，对监测区退耕还林的经济、社会效益进行了评价：退耕还林工程区环境效益经济值明显提高，水土流失得到有效控制；工程区退耕农户收入增加，粮食总产量稳定；退耕还林促进了农村劳动力的转移。  相似文献   

Salix tetradenia Hand.‐Mazz: a new natural plant host of ‘Candidatus phytoplasma’     

H.‐Q. Mou  X. Xu  R.‐R. Wang  Q. Tian  Y. Wei  S.‐F. Zhu  X.‐L. Liao  W.‐J. Zhao 《Forest Pathology》2014,44(1):56-61

This article reports Salix tetradenia Hand.‐Mazz as a new host of Candidatus phytoplasma and demonstrates its association with witches' broom disease on S. tetradenia plants. Plants exhibited typical visual symptoms of phytoplasma with virescence, abnormality of flowers and witches' broom, and phytoplasma bodies were observed by transmission electron microscopy. Products of 1.2 kb were amplified by nested PCR using phytoplasma universal primer pairs R16F2n/R16R2, but no amplification products were obtained from symptomless plants. The sequence analysis of three 16S rDNA isolates showed 99.84%, 99.68% and 99.76% identify, respectively, with the homologous gene (nc_005303) of member of ‘Candidatus phytoplasma asteris’ (16SrI) group. Phylogenetic and virtual computer‐simulated restriction fragment length polymorphism analysis of the 16S rRNA, tuf and rp gene sequences confirmed that this phytoplasma clustered in the 16SrI‐B subgroup. These results indicated that the diseased S. tetradenia plants were infected by a phytoplasma of the 16SrI group. This is the first report on the occurrence of phytoplasma disease on S. tetradenia worldwide.  相似文献   

基于检测技术和PLC的窖储白菜自动控制系统     

赵昌龄  申勋业  杨家鹏 《农机化研究》2010,32(9)

随着农业生产的飞速发展,大规模的窖储白菜已出现在农村的生产中.降低白菜在窖内的损耗率已成为实际农业生产生活的重要问题.由于PLC具有可靠性高、便于扩展等特点,已逐渐代替了传统继电器控制的方式,检测技术和PLC应用于白菜窖储系统可实现该系统的功能要求.为此,介绍了检测技术和PLC在白菜窖储控制系统中的作用与功能的实现.  相似文献   

昆虫生长调节剂的开发现状与发展趋势   总被引：11，自引：0，他引：11  

刘长令 《农药科学与管理》1998,19(3):29-31,23

本文简要地介绍了与环境友好，相容的昆虫生长调节剂包括几丁质抑制剂，保幼激素类似物和蜕皮激素似物的开发现状和发展趋势，昆虫生长调节剂的发展虽缓慢，但前景广阔。  相似文献   

生猪屠宰前囊虫病的检验方法     

张春范姜常令  贺红军 《中国动物检疫》2001,18(7):40-40

猪囊虫病是人畜共患循环感染的寄生虫病，近年来，随着早期诊断、虫苗免疫和治疗技术的不断提高和完善，该病呈逐年下降趋势，主要在农村散养猪中发生。 在生猪屠宰检疫工作中，囊虫病是重点检疫对象，而县乡的屠宰场点是个体收猪集中屠宰，屠宰户在购猪时用舌检法检查猪囊虫病，检出率低，并需一定的经验，宰后检验发现囊虫病猪予以销毁，使屠宰户蒙受很大经济损失。为提高宰前检疫效果，我们采取超前服务措施，应用定量血片间接血凝反应诊断猪囊虫病，先检验、后买猪，或者是先定性、后屠宰，具体做法是向屠宰户发放检验用滤纸条若干，在…  相似文献