全文获取类型
收费全文 | 1907篇 |
免费 | 142篇 |
国内免费 | 1篇 |
专业分类
林业 | 86篇 |
农学 | 36篇 |
基础科学 | 10篇 |
233篇 | |
综合类 | 403篇 |
农作物 | 84篇 |
水产渔业 | 134篇 |
畜牧兽医 | 853篇 |
园艺 | 48篇 |
植物保护 | 163篇 |
出版年
2023年 | 24篇 |
2022年 | 28篇 |
2021年 | 44篇 |
2020年 | 64篇 |
2019年 | 68篇 |
2018年 | 46篇 |
2017年 | 62篇 |
2016年 | 53篇 |
2015年 | 41篇 |
2014年 | 56篇 |
2013年 | 74篇 |
2012年 | 132篇 |
2011年 | 131篇 |
2010年 | 72篇 |
2009年 | 64篇 |
2008年 | 106篇 |
2007年 | 119篇 |
2006年 | 118篇 |
2005年 | 100篇 |
2004年 | 93篇 |
2003年 | 82篇 |
2002年 | 77篇 |
2001年 | 36篇 |
2000年 | 33篇 |
1999年 | 23篇 |
1998年 | 14篇 |
1997年 | 6篇 |
1996年 | 14篇 |
1995年 | 7篇 |
1994年 | 8篇 |
1993年 | 14篇 |
1992年 | 5篇 |
1991年 | 14篇 |
1990年 | 13篇 |
1989年 | 18篇 |
1988年 | 14篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 11篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1980年 | 6篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1976年 | 9篇 |
1972年 | 7篇 |
1971年 | 7篇 |
1969年 | 6篇 |
1967年 | 5篇 |
1955年 | 4篇 |
排序方式: 共有2050条查询结果,搜索用时 31 毫秒
1.
2.
3.
This study was the first to examine the seroprevalence of Ornithobacterium rhinotracheale (ORT) within a commercial egg layer population. Serum samples collected from egg production companies were examined by serum plate agglutination test (SPAT) and outer membrane protein-based enzyme-linked immunosorbent assay (ELISA). Results show that 90% of layer flocks were positive by SPAT and 100% by ELISA. Of the pullet flocks examined, 43% and 52% were positive by SPAT and ELISA, respectively. Our study indicates that the prevalence of ORT antibody is high in the commercial layer population, suggesting that this respiratory pathogen can easily spread through multiple-age layer farms from older flocks to newly housed pullet flocks. 相似文献
4.
1. A 31P topical magnetic resonance study of whole chicken's eggs in vivo is described.
2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.
3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.
4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs. 相似文献
5.
Kevin Kline PhD Leonard P. Frey PhD Jonathan H. Foreman DVM MS Joseph T. Lyman DVM MS 《Journal of Equine Veterinary Science》2005,25(8):349-354
Changes in blood gases, pH, and plasma electrolyte concentrations in response to intravenously infused sodium bicarbonate (NaHCO3) and sodium acetate (NaCH3CO2) solutions (1.34 mEq/mL) in 5 light breed mares were investigated. Jugular venous blood samples were collected before and after completion of the infusions in 20-minute intervals for 200 minutes. Infusion of sodium bicarbonate and sodium acetate caused significant (P < .00l) increases in blood pH and bicarbonate ion concentration that persisted throughout the collection period. The elevation in blood pH and bicarbonate ion concentrations was greater (P < .01) for sodium bicarbonate than for sodium acetate immediately after the completion of the infusions but was not different (P > .05) thereafter. There were significant reductions (P < .01) in plasma-ionized calcium and potassium after infusion of both sodium bicarbonate and sodium acetate. This study found that significant metabolic alkalosis in horses and corresponding shifts in electrolyte concentrations can be induced by intravenous infusion of solutions of either sodium bicarbonate or sodium acetate, and they persist for at least 3 hours. These data show that the short-term elevation in pH and bicarbonate ion concentration is momentarily higher after infusion of sodium bicarbonate. This is likely due to the direct infusion of bicarbonate ions in the sodium bicarbonate treatment, such that further metabolism is not required to be effective. However, the longer-term alkalosis did not differ between isomolar solutions of sodium bicarbonate and sodium acetate. 相似文献
6.
Julia B. Ewaschuk Jonathan M. Naylor Manuel Chirino-Trejo Gordon A. Zello 《Canadian journal of veterinary research》2004,68(4):249-253
Diarrhea is a common occurrence in neonatal calves. Several veterinary probiotics claiming to prevent or treat calf diarrhea are available, but have not been well studied. This study assessed the capability of Lactobacillus rhamnosus strain GG (LGG) to maintain viability in the gastrointestinal tract of calves. We also determined whether LGG can be administered in an oral rehydration solution (ORS) without compromising the efficacy of the ORS or the viability of LGG, and whether LGG produces D-lactate or not. To investigate the intestinal survival of LGG, 15 calves were randomized into 3 groups and LGG was administered orally with their morning milk feeding on 3 consecutive days at a low (LD), medium (MD), or high (HD) dosage. Fecal samples were collected on days 0 (control), 1, 2, 3, 5, and 7 and incubated for 72 h on deMan, Rogosa, Sharpe agar. Twenty-four hours after the 1st feeding, LGG was recovered from 1 out of 5 calves in the LD group, 4 out of 5 calves in the MD group, and 5 out of 5 calves in the HD group. To determine if LGG caused the glucose levels in the ORS to drop below effective levels, 1.5 L of the ORS was incubated with LGG for 2 h at 37 degrees C and the glucose concentration was measured every 20 min using a glucose meter. This ORS was then further incubated for 10 h and aliquots analyzed by high performance liquid chromatography to determine if D-lactate was produced by LGG. Glucose concentrations did not change over the 2 h of incubation, and no D-lactate was produced after 48 h. The LGG maintained viability in ORS. Therefore, this study demonstrated that LGG survives intestinal transit in the young calf, produces no D-lactate, and can be administered in an ORS. 相似文献
7.
Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease 下载免费PDF全文
E. Milne Y. Martinez Pereira C. Muir T. Scase D. J. Shaw G. McGregor L. Oldroyd E. Scurrell M. Martin C. Devine H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271
Objectives
To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.Materials and Methods
Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.Results
Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.Clinical Significance
An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted. 相似文献8.
Laryngeal mask airway and transient hypercapnic hyperpnea for video‐endoscopic assessment of unilateral laryngeal paralysis in dogs 下载免费PDF全文
9.
Distribution,characteristics and condition of Arctic charr (Salvelinus alpinus) spawning grounds in a differentially eutrophicated twin‐basin lake 下载免费PDF全文
10.
Soybean (Glycine max [L.] Merr.) is cultivated primarily for its protein and oil in the seed. In addition, soybean seeds contain nutraceutical compounds such as tocopherols (vitamin E), which are powerful antioxidants with health benefits. The objective of this study was to identify molecular markers linked to quantitative trait loci (QTL) that affect accumulation of soybean seed tocopherols. A recombinant inbred line (RIL) population derived from the cross ‘OAC Bayfield’ × ‘OAC Shire’ was grown in three locations over 2 years. A total of 151 SSR markers were polymorphic of which a one‐way analysis of variance identified 42 markers whereas composite interval mapping identified 26 markers linked to tocopherol QTL across 17 chromosomes. Individual QTL explained from 7% to 42% of the total phenotypic variation. Significant two‐locus epistatic interactions were identified for a total of 122 combinations in 2009 and 152 in 2010. The multiple‐locus models explained 18.4–72.2% of the total phenotypic variation. The reported QTL may be used in marker‐assisted selection (MAS) to develop high tocopherol soybean cultivars. 相似文献