Estrogen and progesterone receptor status of mammary carcinomas and correlation with clinical outcome in dogs.   总被引：3，自引：0，他引：3  

E A Sartin  S Barnes  R P Kwapien  L G Wolfe 《American journal of veterinary research》1992,53(11):2196-2200

Estrogen and progesterone receptors (ER, PR) were measured in cytosol fractions from 18 primary canine mammary carcinomas by use of biochemical assays. One or both receptors were detected (> 10 fmol/mg of cytosol protein) in 11 tumors: 5 ER and PR; 2 ER only; 4 PR only. Mean cytoplasmic receptor concentrations (fmol/mg of cytosol protein) were 22.8 +/- 2.9 (SEM) for ER and 51.0 +/- 10.3 for PR in tumors containing ER and PR, 28.8 +/- 12.1 for ER in tumors containing only ER and 13.2 +/- 1.5 for PR in tumors containing only PR. Estrogen or progesterone receptors or both were identified in 6 of 9 tubular adenocarcinomas, 4 of 5 papillary adenocarcinomas, and 1 of 1 squamous cell carcinoma. These receptors were not identified in solid carcinomas (n = 2) or a single spindle cell carcinoma. Although the number of cases was limited, survival times of dogs tended to be longest in those with tumors containing ER alone or in combination with PR, intermediate in those with tumors containing only PR, and shortest in those with tumors without ER or PR. A correlation was not apparent between receptor status and age, presence of ovaries, tumor size, or histologic classification of the tumor. In the analysis of this series, the extent of surgery (mastectomy of the involved gland vs unilateral or bilateral mastectomy) did not appear to influence the outcome of the disease, and metastasis to regional lymph nodes did not appear to be a reliable prognostic indicator.  相似文献   

0–7–21 Radiation Therapy for the Treatment of Canine Oral Melanoma     

Karen E. Bateman DVM  MSc    Pamela A. Catton  Paul W. Pennock  Stephen A. Kruth 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):267-272

Eighteen dogs with malignant melanoma of the oral cavity were treated with high-dose per fraction (0–7–21) radiation therapy. Eight hundred cGy was administered on days 0, 7, and 21 for a total dose of 2,400 cGy in 3 weeks. Of 17 dogs evaluated, 9 (53%) had a complete remission and 5 (30%) achieved a partial remission with an overall response rate of 83%. Local failure occurred in 2 of the 9 dogs where a complete response was initially observed. One dog died of intercurrent disease, and one died of metastatic disease without evidence of local recurrence. Five dogs are alive and free of disease 9 to nineteen months from the initiation of therapy. The 0–7–21 protocol was well-tolerated, and acute radiation reactions were low-grade and limited to the skin. The results of this study demonstrate that oral melanomas in dogs are responsive to radiation. 0–7–21 radiation therapy offers a viable alternative to radical excision, especially when tumor volume or location would require cosmetically or functionally debilitating surgery.  相似文献   

Effects of an induced pause in egg production and supplementation of the diet with iron on egg shell colour,quality and performance of brown egg layers     

H. Karunajeewa  S. Abu‐Serewa  Pamela A. Harris 《British poultry science》1989,30(2):257-264

1. A pause in egg production was induced in brown‐egg laying hens, aged 68 weeks, by feeding them whole oats for 7 d. Subsequently, these hens, together with control groups, were given laying diets with either 400 or 600 mg/kg iron. The 200 mg/kg of supplemental iron was added in the form of either ferrous sulphate or iron proteinate (chelated iron).

2. The feeding of whole oats halted egg production in 5.7 ±0.22 d and the duration of the pause was 8.3 ±0.78 d.

3. The induction of a pause improved the subsequent rate of egg production, egg output, efficiency of food utilisation, albumen quality, egg specific gravity and shell colour. It also increased food intake and body weight gain but neither egg weight nor the rate of mortality were affected.

4. Neither supplementation of the diet with iron nor its source had any significant effect on laying performance, egg quality or shell colour.  相似文献   

Serum lipase determination in the dog: a comparison of a titrimetric method with an automated kinetic method     

Walter GL  McGraw P  Tvedten HW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(1):23-27

An enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2-diglyceride by lipase. The manual method was the Cherry-Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital-induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneumonia.  相似文献   

Total hemolytic complement values in normal and diseased dog populations     

Wolfe JH  Halliwell RE 《Veterinary immunology and immunopathology》1980,1(3):287-298

The optimum conditions for handling and storage of canine sera for total hemolytic complement assays were assessed. Sera from 113 normal dogs and 217 clinical admissions to the University of Pennsylvania Veterinary Hospital were assayed for total hemolytic complement levels. Normal sera had a mean value of 185 CH50 units. Sera from animals with systemic lupus erythematosus and hemorrhagic gastroenteritis, had significantly lower mean levels of complement than the normal group. Sera from dogs with generalized demodectic mange, tumors, various inflammatory diseases, hypothyroidism, seborrhea, and rheumatoid arthritis had values significantly higher than the normal population.  相似文献   

Detection of target spot and bacterial spot diseases in tomato using UAV-based and benchtop-based hyperspectral imaging techniques     

Abdulridha  Jaafar  Ampatzidis  Yiannis  Kakarla  Sri Charan  Roberts  Pamela 《Precision Agriculture》2020,21(5):955-978

Early and accurate diagnosis is a critical first step in mitigating losses caused by plant diseases. An incorrect diagnosis can lead to improper management decisions, such as selection of the wrong chemical application that could potentially result in further reduced crop health and yield. In tomato, initial disease symptoms may be similar even if caused by different pathogens, for example early lesions of target spot (TS) caused by the fungus Corynespora cassicola and bacterial spot (BS) caused by Xanthomonas perforans. In this study, hyperspectral imaging (380–1020 nm) was utilized in laboratory and field (collected by an unmanned aerial vehicle; UAV) settings to detect both diseases. Tomato leaves were classified into four categories: healthy, asymptomatic, early and late disease development stages. Thirty-five spectral vegetation indices (VIs) were calculated to select an optimum set of indices for disease detection and identification. Two classification methods were utilized: (i) multilayer perceptron neural network (MLP), and (ii) stepwise discriminant analysis (STDA). Best wavebands selection was considered in blue (408–420 nm), red (630–650 nm) and red edge (730–750 nm). The most significant VIs that could distinguish between healthy leaves and diseased leaves were the photochemical reflectance index (PRI) for both diseases, the normalized difference vegetation index (NDVI850) for BS in all stages, and the triangular vegetation index (TVI), NDVI850 and chlorophyll index green (Chl green) for TS asymptomatic, TS early and TS late disease stage respectively. The MLP classification method had an accuracy of 99%, for both BS and TS, under field (UAV-based) and laboratory conditions.

  相似文献   

Evidence for genetic distinction among sympatric ecotypes of Arctic char (Salvelinus alpinus) in south‐western Alaskan lakes          下载免费PDF全文

Shannan L. May‐McNally  Thomas P. Quinn  Pamela J. Woods  Eric B. Taylor 《Ecology of Freshwater Fish》2015,24(4):562-574

Resource polymorphism may play an important role in the process of speciation. The Arctic char (Salvelinus alpinus) exhibits great phenotypic and genetic diversity across its range, making it an ideal species for studies of resource polymorphism and divergence. Here, we investigated genetic variation at 11 microsatellite loci among 287 Arctic char from five isolated yet proximate postglacial lakes in south‐western Alaska that were previously examined for resource polymorphism. Significant differences in pairwise F_ST were detected among all lakes (range from 0.05 to 0.28, all P < 0.02). In one lake (Lower Tazimina Lake), we found evidence for two genetic groups of char and for significant differences in the distribution of microsatellite variability among at least two of the three previously described body size morphotypes (‘large’‐, ‘medium’‐, and ‘small’‐bodied char; maximum F_ST = 0.09; differences in admixture proportions). We also found a significant association between genetic admixture proportions and gill raker counts among body size morphs (r = ?0.73, P < 0.001). Our data represent the first record of genetically distinct sympatric morphs of Arctic char in Alaska and provide further evidence that differences in morphology associated with feeding (gill rakers) and growth trajectories reflect niche diversification and promote genetic divergence in Holarctic populations of Arctic char.  相似文献   

Randomized controlled clinical study evaluating the efficacy and safety of intratumoral treatment of canine mast cell tumors with tigilanol tiglate (EBC‐46)     

Thomas R. De Ridder  Justine E. Campbell  Cheryl Burke‐Schwarz  David Clegg  Emily L. Elliot  Samuel Geller  Wendy Kozak  Stephen T. Pittenger  Jennifer B. Pruitt  Jocelyn Riehl  Julie White  Melissa L. Wiest  Chad M. Johannes  John Morton  Pamela D. Jones  Peter F. Schmidt  Victoria Gordon  Paul Reddell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):415-429

  相似文献   

Effect of the Nixtamalization Process on the Dietary Fiber Content,Starch Digestibility,and Antioxidant Capacity of Blue Maize Tortilla     

Luis A. Bello‐Prez  Pamela C. Flores‐Silva  Gustavo A. Camelo‐Mndez  Octavio Paredes‐Lpez  Juan de Dios Figueroa‐Crdenas 《Cereal Chemistry》2015,92(3):265-270

Nixtamalization is an ancient process developed by the Mesoamerican cultures. Initially, volcanic ashes were used and then calcium hydroxide in commercial production, and more recently nixtamalization with calcium salts (NCS) has been proposed. The aim of this study was to evaluate the effect of NCS on carbohydrate digestibility and antioxidant capacity in the elaboration of blue maize tortillas. NCS in blue tortillas showed a high amount of total dietary fiber (14.27 g/100 g), the main fraction being insoluble dietary fiber. The contents of resistant starch and slowly digestible starch did not change with the nixtamalization process. The predicted glycemic index value was lower in blue tortillas with the NCS process (58) than with the traditional nixtamalization process (71). In general, NCS in blue tortillas presented a higher antioxidant capacity than traditional tortillas (ferric reducing antioxidant power method), indicating that phenolics present in blue maize maintain their activity after cooking. It can be concluded that the nutraceutical features (high dietary fiber content and antioxidant capacity) of blue maize tortillas are enhanced when they are elaborated with the NCS process.  相似文献   

Incidence and effects of West Nile virus infection in vaccinated and unvaccinated horses in California     

Gardner IA  Wong SJ  Ferraro GL  Balasuriya UB  Hullinger PJ  Wilson WD  Shi PY  MacLachlan NJ 《Veterinary research》2007,38(1):109-116

A prospective cohort study was used to estimate the incidence of West Nile virus (WNV) infection in a group of unvaccinated horses (n = 37) in California and compare the effects of natural WNV infection in these unvaccinated horses to a group of co-mingled vaccinated horses (n = 155). Horses initially were vaccinated with either inactivated whole virus (n = 87) or canarypox recombinant (n = 68) WNV vaccines during 2003 or 2004, prior to emergence of WNV in the region. Unvaccinated horses were serologically tested for antibodies to WNV by microsphere immunoassay incorporating recombinant WNV E protein (rE MIA) in December 2003, December 2004, and every two months thereafter until November 2005. Clinical neurologic disease attributable to WNV infection (West Nile disease (WND)) developed in 2 (5.4%) of 37 unvaccinated horses and in 0 of 155 vaccinated horses. One affected horse died. Twenty one (67.7%) of 31 unvaccinated horses that were seronegative to WNV in December, 2004 seroconverted to WNV before the end of the study in November, 2005. Findings from the study indicate that currently-available commercial vaccines are effective in preventing WND and their use is financially justified because clinical disease only occurred in unvaccinated horses and the mean cost of each clinical case of WND was approximately 45 times the cost of a 2-dose WNV vaccination program.  相似文献