古籍咨询工作中的新视角     

卜林  张杰 《农业图书情报学刊》2009,21(8):71-73

本文讨论古籍咨询的服务创新问题,提出了内容咨询这一新概念。认为根据个人自身条件,有些咨询人员不妨可以对古籍的内容进行研究,这有利于与读者进行深入的交流,可以提高咨询水平的深度。  相似文献   

Leptin对动物繁殖机能的影响及其应用前景   总被引：2，自引：0，他引：2  

张海艳  岳文斌 《畜牧兽医杂志》2003,22(3):14-16

Leptin是由脂肪组织分泌的一种“厌食”激素。自1995年发现以来，就成为最活跃的研究领域。它可通过下丘脑或直接作用于卵巢，对动物青春期的发育、生殖器官的发育以及性激素的分泌都有重要的调节作用。本文综述了Leptin的生物学特性、作用机理及其在繁殖方面的作用，井指出了它在畜牧生产中应用前景。  相似文献   

天然芸苔素内酯缓解胺苯磺隆对后茬水稻药害的作用机理初探   总被引：4，自引：0，他引：4       下载免费PDF全文

周小毛  柏连阳  刘雪源  任新国  李双祁  卜小莉  黄柯程 《农药学学报》2003,5(1):61-67

通过测定天然芸苔素内酯对水稻幼苗谷胱甘肽(GSH)含量、谷胱甘肽-S-转移酶(GST)活性以及在离体和活体条件下对水稻幼苗乙酰乳酸合成酶(ALS)活力的影响,研究天然芸苔素内酯减轻胺苯磺隆对水稻药害的作用机理。结果表明,其作用机理与水稻幼苗GSH含量、GST活性变化没有太大相关性,可能与其能间接激活水稻幼苗ALS活力有较大相关。  相似文献   

Activation of renal tubular epithelial cells by monocytes/macrophages and the relative mechanisms     

YANG Li  LI Xiao-mei  WANG Rong  WANG Hai-yan 《园艺学报》2002,18(10):1217-1221

AIM: To observe the direct effects of peripheral blood monocytes/macrophages (MO/MAC) on renal tubular epithelial cells (RTEC),and further probe into the possible mechanisms. METHODS: Conditioned medium(M-CM) of human peripheral blood MO/MAC was collected and added to HK-2,a human renal proximal tubular cell line.After incubation with M-CM for 24 hours,HK-2 cells were detected for DNA synthesis by [³H]-TdR incorporation,osteopontin (OPN) and α-smooth muscle actin (α-SMA) expression by Western blot,and fibronectin(FN) secretion by ELISA.Furthermore,anti-TGFβ₁ neutralizing antibody and interlukin-10(IL-10) were used separately to antagonize the effects of M-CM on HK-2 cells. RESULTS: ①DNA synthesis,α-SMA expression and FN secretion were all increased in HK-2 cells when incubated with M-CM.②When adding with anti-TGFβ₁ neutralizing antibody (5 mg/L) in the M-CM,the degree of upregulation of α-SMA and FN in HK-2 cells was much lower than that stimulated by M-CM alone.③M-CM added with IL-10 (20 μg/L) had a weaker ability to induce the increasing in α-SMA expression and FN excretion in HK-2 cells, compared with M-CM itself alone.M-CM from MO/MAC preincubated with IL-10 caused a lower upregulation of α-SMA expression in HK-2 cells than M-CM from non-preincubated MO/MAC. CONCLUSION: MO/MAC can directly induce proliferation,transdifferentiation and extracellular matrix secretion in RTEC.TGFβ₁ and proinflammatory cytokines secreted by MO/MAC might be involved in the aboveeffects.  相似文献   

苹果渣发酵料栽培糙皮侧耳优良菌株的选育   总被引：2，自引：0，他引：2  

卜庆梅  王淑芳  王书卿  黄清荣 《中国食用菌》2002,21(4):10-12

新选育的糙皮侧耳菌株PL -A2 属于早熟型品种。该菌株菌丝在 5～ 37℃温度范围内均能生长 ,菌丝生长适温为 18～ 30℃ ,最适温度为 2 5～ 2 8℃。在苹果渣发酵料培养基上 ,菌丝粗壮、整齐、洁白 ,爬壁能力强 ,满袋天数为 2 2d± 1d。经推广栽培证明 ,PL -A2 是高产优质菌株  相似文献   

蔗渣滤泥培养料栽培平菇试验   总被引：2，自引：0，他引：2  

刘晓波  李海燕  赵鹏  陈朝银 《中国食用菌》2002,21(6):17-18

以不同比例滤泥代替栽培料中的麦麸进行平菇栽培试验。结果表明 ,滤泥能替代栽培料中的麦麸。  相似文献   

杏鲍菇优良品种HRX的选育研究   总被引：3，自引：1，他引：3  

王淑芳  卜庆梅  辛晓林  杨立红 《中国食用菌》2003,22(3):10-12

通过对杏鲍菇优质菌株HRX3定向驯化试验,结果表明:经过热处理的菌株,其对温度的耐受性能有明显改善;提高培养基中的木质素和纤维素成分,可以增强菇体内相关酶的活性;并筛选出适合本地栽培该菇的培养基质.  相似文献   

灰树花不同配方栽培研究   总被引：4，自引：0，他引：4  

卜庆梅  王淑芳  梁建光  胡奎娟 《中国食用菌》2003,22(6):23-24,39

对灰树花七种栽培配方进行了研究，其结果表明：木屑和棉籽壳作为栽培的主要原料，若等比例混合。生物学效率最高；麦麸与玉米粉搭配使用，可以提高灰树花的产量及生物学效率；平菇废料是栽培灰树花较好的原料，并具有很好的经济效益、社会效益和生态效益；并筛选出灰树花高产的三个配方。  相似文献   

莫莫格湿地干-湿界面优势植物种群动态分析   总被引：1，自引：0，他引：1  

田迅  卜兆君 《四川草原》2004,(11)

莫莫格湿地从高河漫滩底端到顶端选取有代表性的植物群落片断作为固定观测样带,并设置6个观测样方,生长季5月、7月、9月3次观测结果表明,小叶章优势种群5月份密度最高达1154-1594株/平方米,7月种群数量下降,9月有所升高;踏头苔草优势种群5-7月份密度在3 414-7 193株/平方米,9月份种群数量大幅度下降;土壤相对含水量低地段小叶章种群相对优势度大,土壤相对含水量高地段踏头苔草种群相对优势度大,局部地段生长季内有相对优势度相互替代过程,莫莫格湿地干-湿界面上小叶章和踏头苔草两个种群可以共存。  相似文献   

Comparison of different conditions inducing embryonic stem cells     

QIAN Hai-yan  LI Geng-shan  XU Hong-xin  L&#  Jing-jun  JIANG Wen  WANG Jing 《园艺学报》2004,20(12):2198-2201

AIM: To evaluate the different conditions inducing mouse embryonic stem cells (ESC) in vitro to differentiate into cardiomyocytes. METHODS: BRL conditioned medium was used to promote the growth of ESC and maintain them in an undifferentiated state. During the inducing process, retinoic acid (RA), DMSO, activin-A and TGF-β₁ were used as inducing reagents, and made up six kinds of differentiating medium. Then a three-step method inducing ESC cultured in hanging drops, in suspension and in plating was used to induce the differentiation of ESC. RESULTS: ESC were induced in vitro to differentiate into cardiomyocytes. Of all groups, the highest differentiating rate was observed in the group induced by activin-A (20 μg/L) and TGF-β₁ (2 μg/L). CONCLUSION: The inducing conditions including activin-A (20 μg/L) and TGF-β₁ (2 μg/L) is very valuable in inducing ESC differentiation into cardiomyocytes.  相似文献