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Summary Pollen from the inbred maize line HMv 1645 was used to study the effect of pollen treatments (drying and deep-freezing) on the phenotypic performance of the next generation. Fresh and artificially dried pollen samples with different water contents (56%, 18%, 13% and 10%) were used for sib pollinations immediately after collection or drying. Samples containing low amounts of water were then stored in liquid nitrogen for 7 days. Fertilization ability of the samples with 13% water was the highest after storage. Plant characteristics of the next generation originated from the seeds set by differently treated (fresh, dried to 13% water and deep-frozen) pollen were examined and statistically analysed. Pollen treatments due to the pollen storage procedure did not cause detectable changes in quantitative characters of the next generation. 相似文献
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Effect of parental genotypes and colchicine treatment on the androgenic response of wheat F1 hybrids
I. Zamani E. Gouli-Vavdinoudi G. Kovacs I. Xynias D. Roupakias B. Barnabas 《Plant Breeding》2003,122(4):314-317
The effect of the parental genotypes and colchicine treatment on the androgenic response of wheat (Triticum aestivum L.) F1 hybrids was studied. For this, anthers from three F1 hybrids and their parents were cultured on W14 initiation medium and W14 supplemented with 0.03% colchicine. The number of responding anthers, microspore‐derived structures/100 anthers, green plants/embryos cultured, green plants/100 anthers and albino plants/100 anthers were recorded. It was observed that embryo formation and plant regeneration ability were genetically controlled and genotype dependent. In both treatments the variety Kavkaz had a significantly higher percentage of responding anthers, microspore‐derived structures and green plants/100 anthers than the other genotypes. On the other hand, the variety Myconos also demonstrated high microspore‐derived structure production and green plant regeneration when treated with colchicine. The good response observed in these two varieties indicates the importance of colchicine treatment only for certain genotypes. Green plant production capacity of the hybrids was intermediate to that of the parental varieties. As one parent with a high or even an intermediate response to anther culture could lead to the production of sufficient (for breeding purposes) green plants from the F1 hybrids, it was concluded that screening the inbred lines for the response to anther culture with and without colchicine treatment could contribute to utilization of breeding material with a low response to anther culture via the proper hybrid combinations. 相似文献
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Bouzouidja Ryad Béchet Béatrice Hanzlikova Jitka Sněhota Michal Le Guern Cécile Capiaux Hervé Jean-Soro Liliane Claverie Rémy Joimel Sophie Schwartz Christophe Guénon René Szkordilisz Flora Körmöndi Barnabas Musy Marjorie Cannavo Patrice Lebeau Thierry 《Journal of Soils and Sediments》2021,21(5):1909-1927
Journal of Soils and Sediments - In urban areas, soil functions are deeply impacted by all human activities, e.g., water infiltration, carbon storage, and chemical substances degradation potential.... 相似文献
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N. M. R. Ashwin E. Leonard Barnabas A. Ramesh Sundar M. Muthumeena P. Malathi R. Viswanathan 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(2):285-297
Red rot caused by Colletotrichum falcatum is one of the most devastating fungal diseases of sugarcane in many tropical countries. In this study, the efficacy of various resistance-inducing agents was evaluated against red rot at two growth phases of sugarcane, i.e., the germination and grand growth phases, for four consecutive cropping seasons (2011–12 to 2014–15) under field conditions. Inducer candidates of both synthetic origin (benzothiadiazole [BTH], salicylic acid [SA], potassium silicate [PSi]) and biotic origin (Colletotrichum falcatum [Cf] elicitor and Reynoutria sachalinensis extract) were evaluated for their efficacy on a red rot susceptible sugarcane cultivar CoC 671. Inducer concentrations that showed significant reduction of red rot lesion length in leaf bioassays without exhibiting direct antifungal activity were used in the field trials. Overall, results of the four field trials indicated that application of BTH (125 μM) and Cf elicitor (60 μg glucose equivalent/ml) were most efficient in reducing disease incidence during the germination and establishment phases against soil-borne inoculum and suppressed disease severity in pathogen challenged cane stalks during the grand growth phase. In addition, other treatments also had significant effects on germination and stalk disease severity. Quantification of C. falcatum biomass in resistance inducer-treated canes by quantitative PCR (qPCR) substantiated the disease suppressive effect of BTH and Cf elicitor. Whereas all resistance inducers significantly increased the cane weight, only BTH and PSi significantly increased the juice quantity. Other juice quality parameters were generally not affected by the inducers. 相似文献
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Anburaj D. Barnabas Girish K. Radhakrishnan Usha Ramakrishnan 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(1):41-51
The virus causing horsegram (Macrotyloma uniflorum) yellow mosaic disease has been shown to be a typical Old World bipartite begomovirus. The viral origin of the disease has
been established through agroinoculation of horsegram using partial tandem repeat clones of both DNA-A and DNA-B. The DNA-A
genome shows less than 89% identity with the corresponding sequences of all the begomoviruses in the databases earlier to
this sequence submission (AJ627904). Therefore Horsegram yellow mosaic virus (HgYMV-[IN:Coi]) can be considered to be a new species of the genus Begomovirus (family Geminiviridae). Phylogenetic analysis shows that this virus is part of the cluster of mungbean yellow mosaic viruses of legumes from South
and South East Asia. 相似文献
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Barnabas Neba Nfornkah Tchamba Martin Louis Zapfack Djomo Chimi Cedric Mairong Nsakii Frederick Bonaventure Sonke 《Journal of Sustainable Forestry》2019,38(7):670-685
ABSTRACTForest Management Unit (FMU) is a growing phenomenon in Cameroon, impacting biodiversity negatively. This study was initiated to assess vascular epiphytes’ species loss on exploited trees to suggest conservation strategies. We hypothesized that: 1) epiphyte loss in this managed forest was significantly high and different with respect to phorophyte species; 2) epiphyte diversity loss has threatened status. Epiphyte survey was conducted on four most exploited trees in this study area. 63 exploited trees (Phorophytes) were surveyed. Shannon, Chao1 and Sorensen’s similarity indexes were used. Kruskal–wallis test was used to test the hypotheses. We recorded (70 epiphyte species, 33 genera and 12 families). Orchidaceae constituted highest epiphyte species with 43 species (61.4%). Epiphytes diversity was similar with respect to different phorophyte species (Sorensen?50%). Shannon’s index (3.36) showed a high epiphyte diversity loss. Kruskal–wallis showed no significant difference in epiphyte loss between phorophytes but a significant difference between zones, with canopies losing the highest epiphytes. With one epiphyte species’ status almost threatened and epiphyte diversity loss (significant); strict sustainable conservation methods like: epiphyte shade-houses and relocation are solicited. More data are needed from similar and different managed forest strata for epiphyte conservation policy enactment in Cameroon. 相似文献
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Barnabas Neba Nfornkah Louis Zapfack Martin Tchamba Cedric Djomo Chimi Bonaventure Sonke 《Journal of Sustainable Forestry》2018,37(6):619-631
Vascular epiphyte biomass is poorly studied in Congo Basin forest. The aim of this study was to develop a protocol to estimate epiphyte biomass. Epiphytes were collected on 77 phorophytes of diameter 57–169.5 cm, oven-dried for biomass. Allometric equations for epiphyte biomass were developed with diameter and phorophyte height as predictor variables using regression analysis. The mean epiphyte biomass was 24.48 kg/phorophyte with a standard deviation of ±49.99. Cylicodiscus gabunensis and Triplochiton scleroxylon hosted the greatest epiphyte biomass with a mean vascular epiphyte biomass of 71.65 and 62.3 kg/phorophyte, respectively. Kruskal–Wallis test (p = 0.005) showed that there was a significant difference between epiphyte biomass with respect to phorophyte species. The relationship found between epiphyte biomass and dendrometric variables of the phorophytes were intermediate (Adj.R2 < 0.630) meaning epiphyte biomass increased with increasing tree diameter. However, models with diameter input variables had greater prediction than input variables of total height. Based on the Akaike Information Criterion (AIC), the allometry lnY = ?21.37 + 5.21 x ln(D) ?0.35 x ln(H) with AIC (183) was the best equation for the prediction of epiphyte biomass. These findings shall contribute to REDD+ (Reducing Emissions from Deforestation and Forest Degradation) strategy for climate change mitigation in Congo Basin. 相似文献
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Yoseph Beyene Stephen Mugo Kassa Semagn Godfrey Asea Walter Trevisan Amsal Tarekegne Tadele Tefera James Gethi Barnabas Kiula John Gakunga Haron Karaya Andrew Chavangi 《Euphytica》2013,192(3):379-392
In contrast to conventional inbreeding that takes up to seven generations to develop inbred lines, the doubled haploid (DH) technology allows production of inbred lines in two generations. The objectives of the present study were to: (a) evaluate testcross performance of 45 doubled haploid lines under drought stress and non-stress conditions (b) estimate heritabilities for grain yield and other traits and (c) to assess the genetic distance and relationship among the DH lines using 163,080 SNPs generated using genotyping-by-sequencing (GBS). The 45 hybrid and five checks were evaluated using a 10 × 5 alpha lattice in six drought stress and nine well-watered environments in Kenya, Uganda, and Tanzania. Differences in trait means between the drought stress and well-watered conditions were significant for all measured traits except for anthesis date. Genetic variances for grain yield, grain moisture, plant height and ear height were high under well-watered environments while genetic variance for anthesis date, root lodging and stalk lodging were high under drought stress environments. Combined analyses across drought stress and well-watered environments showed that ten top hybrids produced 1.6–2.2 t/ha grain yield under well-watered condition and 1–1.4 t/ha under drought stress condition higher than the mean of the commercial checks. Genetic distance between pairwise comparisons of the 38 of the 45 DH lines ranged from 0.07 to 0.48, and the overall average distance was 0.36. Both cluster and principal coordinate analysis using the genetic distance matrix calculated from 163,080 SNPs showed two major groups and the patterns of group was in agreement with their pedigree. Thirteen (13) of the best hybrids are currently in National Performance Trials testing, an important step towards commercialization in Kenya, Tanzania and Uganda. 相似文献
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Keny Vanlalhriatpuia Vanlalnghaka Chhakchhuak Satralkar K Moses SB Iyyer MS Kasture AJ Shivagaje Barnabas J Rajneesh Dilip S Joshi 《Journal of circadian rhythms》2007,5(1):1-11
Background
We recently reported that the altitude of origin altered the photic and thermal sensitivity of the circadian pacemaker controlling eclosion and oviposition rhythms of high altitude Himalayan strains of Drosophila ananassae. The present study was aimed at investigating the effects of altitude of origin on the pacemaker controlling the adult locomotor activity rhythm of D. helvetica. 相似文献
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