哺乳犊牛的消化特点与蛋白质需要   总被引：7，自引：0，他引：7  

李辉  刁其玉 《中国饲料》2005,(21):22-24

本文从犊牛的消化生理特点出发,综述了犊牛出生后的生理特征及蛋白质、必需氨基酸的需要量,并对代乳品中蛋白质原料进行了论述。  相似文献   

代乳粉用于早期断奶犊牛的效果研究   总被引：14，自引：0，他引：14  

刁其玉  张乃锋  刘文忠  祖致富  袁耀明 《乳业科学与技术》2004,27(2):70-73

犊牛使用代乳品可以节省鲜奶，降低饲养成本，控制疾病的发生，促进消化系统的发育。试验用24头犊牛做试验，60日龄断奶。结果表明，代乳品组犊牛增重47．55千克；对照组增重41．72千克；吃代乳品的犊牛在30-60日龄的日增重达到1026克，高于对照组103克。吃代乳品的犊牛每增重1千克的直接饲料成本为15．13元，比鲜奶组犊牛低2．14元／千克。  相似文献   

茶黄酮对无角多赛特羊生长和呼吸道疾病的影响     

张乃锋  刁其玉  丁金芝  唐全生 《中国草食动物》2005,25(5):46-47

选择平均体重为26．6kg的无角多赛特断奶大羔羊22只．平均体重为49．3kg的无角多赛特育成羊48只，分别分为对照组和试验组，试验组羊只精料补充料中添加300mg／kg的茶黄酮，研究茶黄酮对羊只生长和呼吸道疾病的影响。结果表明，添加茶黄酮可提高羔羊和育成羊的增重效果．和对照组比较羔羊提高增量9．8%（P〉0．05），育成羊提高增重6．1%（P〉0．05）；在饲料中添加茶黄酮可使羔羊呼吸道疾病发病率降低64%，使育成羊呼吸道疾病发病率降低50%，可有效预防羊的呼吸道疾病。  相似文献   

棘孢木霉葡聚糖酶基因Glu1的克隆及原核表达     

刁桂萍  杨帅  遇文婧 《草业科学》2018,35(7):1685-1694

从棘孢木霉(Trichoderma asperellum)ACCC30536中克隆获得一个葡聚糖酶基因Glu1,其cDNA全长984bp,编码327个氨基酸。该葡聚糖酶属于Glyco-hydro-12家族,推测为β-1,4-葡聚糖酶,与深绿木霉IMI 206040的Glycohydro-12家族蛋白(XP_013940397.1)有91%相似性,且亲缘关系较近。运用qRT-PCR技术检测9种诱导条件下棘孢木霉Glu1基因的表达水平,结果表明,Glu1基因能参与棘孢木霉对山新杨(Populus davidiana×P.alba var.pyramidlis)或杨树病原菌的识别。构建原核表达载体并获得重组蛋白rGlu1。酶活特性分析结果表明,该酶最适pH为4.5,最适温度为45℃,且酶活性随诱导时间延长逐渐升高,在5h达到稳定。  相似文献   

Effect of urea supplementation on performance and safety in diets of Dorper crossbred sheep          下载免费PDF全文

B. Wang  T. Ma  K.‐D. Deng  C.‐G. Jiang  Q.‐Y. Diao 《Journal of animal physiology and animal nutrition》2016,100(5):902-910

This study was conducted to evaluate the efficacy and safety of dietary urea in sheep rations having a 50:50 concentrate:roughage ratio. Sixty‐four Dorper × thin‐tailed Han crossbred ram lambs with an average body weight of 30.8 (±0.02) kg were randomly divided into four groups of 16 sheep each, and each group was fed one of the following diets: a basal diet (CON), or CON supplemented with 0.5% (0.5UTM), 1.5% (1.5UTM) or 2.5% (2.5UTM) urea. Growth performance, carcass characteristics, non‐carcass offals, meat quality and peptic tissue lesions were assayed. The average daily weight gains for CON, 0.5UTM, 1.5UTM and 2.5UTM were 216, 218, 200 and 170 g, respectively, with the CON and 0.5UTM groups higher than 2.5UTM group (p < 0.05). Sheep from the 2.5UTM treatment had a significantly lower dry matter intake (1.29 kg/day) than those from the CON and 0.5UTM treatments (1.42 and 1.43 kg/day, p < 0.05), and the feed conversion ratio in the 2.5UTM group was the highest (p < 0.05). Carcass characteristics, including shrunk body weight, empty body weight, hot carcass weight, dress percentage, and the absolute or relative weight (% body weight) of heart, liver, spleen, lung and kidney, were not altered by the treatments (p > 0.05). The muscular pH of 2.5UTM was higher than that of CON (5.68 vs. 5.52, p < 0.05), and shear force in 0.5UTM was lower compared with CON and 2.5UTM (p < 0.05). The anatomical structure lesions in kidneys became more serious with the increasing dietary urea concentrations, with the 2.5UTM animals showing the most severe lesions compared with CON animals. Therefore, supplementary urea as a non‐protein nitrogen source for sheep should not exceed 1.5% of ration having a 50:50 concentrate:roughage ratio to ensure efficacy and safety.  相似文献   

Mutant-prevention concentrations of enrofloxacin for Escherichia coli isolates from chickens     

Li Q  Bi X  Diao Y  Deng X 《American journal of veterinary research》2007,68(8):812-815

OBJECTIVE: To investigate the development of enrofloxacin resistance among Escherichia coli isolates obtained from chickens by determining mutant-prevention concentrations (MPCs) and sequence the quinolone resistance-determining regions (QRDRs) of gyrA and parC genes in selected isolates. SAMPLE POPULATION: 15 chicken-derived E coli isolates. PROCEDURES: For all isolates, MPC and minimal inhibition concentration (MIC) of enrofloxacin were determined. The MPCs and maximum serum drug concentrations attained with enrofloxacin doses recommended for treatment of E coli infections in chickens were compared. Mutation frequencies and QRDR sequence changes in gyrA and parC were also determined. RESULTS: In 2 of 15 E coli strains, MPCs were low (0.016 and 0.062 microg/mL), MPC:MIC ratios were 2 and 4, and the GyrA and ParC proteins had no mutations. In 9 susceptible isolates with a GyrA point mutation, MPCs ranged from 2 to 16 microg/mL. For isolates with double mutations in GyrA and a single mutation in ParC, MPCs were > 32 microg/mL (several fold greater than the maximal plasma concentration of enrofloxacin in chickens); mutation frequencies were also much lower, compared with frequencies for single-mutation isolates. CONCLUSIONS AND CLINICAL RELEVANCE: For E coli infections of chickens, MPC appears to be useful for determining enrofloxacin-dosing strategies. The high MPC:MIC ratio may result in enrofloxacin-treatment failure in chickens infected with some wild-type gyrA E coli isolates despite the isolates' enrofloxacin susceptibility (MICs 0.125 to 1 microg/mL). For infections involving isolates with high MPCs, especially those containing mutations in gyrA and parC genes, treatment with combinations of antimicrobials should be adopted.  相似文献   

凹凸棒添加对有机肥发酵温度及养分的影响     

王钰轩  俄胜哲  袁金华  姚佳璇  王少琦  赵天鑫 《农业环境科学学报》2021,40(12):2779-2787

为充分利用凹凸棒石的吸附性能,降低有机肥发酵过程中的碳氮损失,减少臭气排放,设置4个不同凹凸棒石添加量（0、5%、10%、15%）,探讨凹凸棒石添加量对有机肥发酵温度、养分含量及养分损失率的影响。结果显示：相比不添加凹凸棒石的对照,添加凹凸棒石可降低堆肥发酵最高温度,延长高温持续时间,降低堆体水分损失速率; 5%凹凸棒石添加可显著降低氮素损失约10个百分点,但当其添加量超过10%时,氮素损失反而增加;添加凹凸棒石显著降低了有机质含量和损失率,且凹凸棒石添加量与有机质损失率呈显著负相关关系。此外,添加凹凸棒石可提高有机肥的钾素含量,但对磷素的含量和损失无显著影响。研究表明,有机肥生产工艺中,凹凸棒石的适宜添加量应为物料总质量的5%~10%。  相似文献   

正反转组合式水稻宽苗带灭茬播种机设计与试验     

张银平  杜瑞成  刁培松  杨善东  王振伟 《农业工程学报》2017,33(3):7-13

针对稻麦两茬轮作区,小麦收获时间短、水稻插秧费时费力、直播整地要求高的问题,利用反转灭茬技术和主动防拥堵技术,同时借鉴小麦宽幅精播技术,设计了一种正反转组合式水稻宽苗带灭茬播种机,一次完成旋耕、灭茬、防堵、深施肥、宽苗带播种、覆土和镇压功能。在稻麦两熟区进行了试验,结果表明,反转旋耕装置能很好的灭茬,正转清草装置能有效防堵,在正反旋耕的配合作用下,种床土壤细碎,播种覆土均匀,播种深度和施肥深度变异系数分别为4.58%和2.40%,种肥垂直间距变异系数为4.72%;平均苗带宽度为138.4 mm,与理论设计宽度差异不显著;不同苗带宽度上种子分布有差异但不显著,符合设计要求;苗带宽度对水稻生长有影响,苗带两侧有效分蘖和成穗率显著高于苗带中间(P0.05);机具的通过性满足农艺要求。  相似文献   

山东省水稻免耕旱直播试验及可行性分析   总被引：4，自引：3，他引：4  

张银平  杜瑞成  刁培松  杨善东 《农业工程学报》2016,32(12):24-30

为探究水稻免耕旱直播在山东省的适应性,使用2BDMM-8稻麦宽苗带灭茬播种机,在涛雒稻麦轮作两熟区和高青春季稻一熟区分别进行了免耕旱直播试验,以机械育苗插秧为对照,分析免耕旱直播对水稻株高、分蘖情况的影响,对不同种植方式下的水稻产量及产量构成因素、生产成本等进行统计分析。结果表明:水稻免耕旱直播与机械育苗插秧相比,生育期短,生长速度快,株高无显著差异;分蘖率分别为57%和58%,比插秧对照低6.5%和10.4%;产量分别为8 970和10 020 kg/hm2,与插秧对照无显著差异(P0.05),验证了水稻免耕旱直播在山东常规稻种植区基本可行。通过成本比较,免耕旱直播成本比插秧对照分别节约2 472和3 312元/hm2,说明免耕旱直播在山东地区有很好的推广前景。该研究为山东常规稻种植区免耕旱直播提供了参考。  相似文献   

病原微生物药敏检测方法的研究进展     

刁菁  杨秀生  李天保  叶海斌  许拉  樊英  王淑娴 《中国农学通报》2013,29(8)

抗生素敏感试验在当前病原微生物耐药性持续增长的形势下,已成为指导临床科学合理用药不可或缺的重要环节.因此,药敏检测方法的完善与发展是快速获取科学、准确微生物耐药谱的前提,也是实现有效临床治疗的重要保障.本研究介绍了当前应用于微生物药物敏感检测的常规方法,如纸片扩散法、稀释法、Etest法等,简要分析了它们之间的优缺特性,并对近年来一些新兴发展的快速药敏检测方法进行了总结以及展望了不同类型药敏检测方法的发展和应用趋势.  相似文献