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为分析绒山羊JAG1蛋白结构特征,及其在不同毛囊生长期中的表达模式,从阿拉善种羊场选择2周岁、体重相近的3只内蒙古绒山羊母羊,利用生物信息学方法比对绒山羊JAG1氨基酸序列与其他物种的相似性,分析JAG1蛋白质理化性质和结构特征;利用免疫组化和实时荧光定量PCR检测其在内蒙古绒山羊皮肤中的表达部位和表达量。结果表明,绒山羊JAG1氨基酸序列与绵羊相似性可达99.5%,JAG1蛋白信号肽位于第1~29个氨基酸序列,含有一个剪切位点和一个跨膜结构,具有61个丝氨酸、26个苏氨酸、19个酪氨酸磷酸化位点,63个O-糖基化位点,6个N-糖基化位点,属于不稳定亲水蛋白。在毛囊退行期(1月份)和休止期(4月份)未检测到JAG1蛋白的表达;生长前期(7月份)和生长期(9月份)在毛乳头细胞中表达。JAG1 mRNA在内蒙古绒山羊皮肤毛囊的4个时期均有表达,随着毛囊进入生长期JAG1 mRNA表达量逐渐增加,生长期皮肤中的表达量显著高于其他阶段(P<0.05)。综上,绒山羊JAG1氨基酸序列在物种进化过程中比较保守,JAG1 mRNA在内蒙古绒山羊毛囊生长前期和生长期表达升高,为深入研究绒山羊JAG1基因对毛囊发育的调控提供理论依据。 相似文献
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Wang D Nanding H Han N Chen F Zhao G 《Journal of agricultural and food chemistry》2008,56(4):1495-1500
Six model compounds having a 2-(1 H-pyrrolyl)carboxylic acid moiety and a hydrophobic R group were synthesized to study their effects on garlic greening, the structures of which are similar to that of 2-(3,4-dimethyl-1 H-pyrrolyl)-3-methylbutanoic acid (PP-Val) (a possible pigment precursor for garlic greening). The puree of freshly harvested garlic bulbs turned green after being soaked in solutions of all these compounds, and with both increasing concentrations and incubation time the green color of the puree became deeper. In contrast, neither pyrrole alone nor pyrrole combined with free amino acids had the ability to discolor the puree. The compounds exhibited a good relationship between structure and activity of garlic greening, namely, the smaller the size of the R group, the larger the contribution. Also, it was found that the unidentified yellow species can be produced by reacting the model compounds with pyruvic acid at room temperature (23-25 degrees C). Moreover, blue species were formed by incubation of the model compounds with di(2-propenyl) thiosulfinate at room temperature. On the basis of these observations, a pathway for garlic greening was proposed. 相似文献
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WU Jiang-hong ZHANG Yan-jun ZHANG Jia-xin CHANG Zi-li LI Jin-quan YAN Zu-wei Husile ZHANG Wen-guang 《农业科学学报》2012,12(7):1159-1166
Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression. 相似文献
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绵羊BMP15基因三个位点核苷酸多态性研究 总被引：1，自引：0，他引：1
研究目的:采用PCR-SSCP技术分析绵羊BMP15基因exon 1、B2、B4位点在蒙古羊中的多态性,分析其多态性与群体繁殖性状之间的关系.研究意义:BMP15基因是与绵羊繁殖性状有关的基因,它的多态性与群体遗传之间的研究将对绵羊的育种与繁殖有重要意义.研究结果:结果表明在exon 1所扩增的片段中存在3种基因型(AA型、BB型、AB型),在B2位点所扩增的片段中不存在多态性,B4扩增的片段中存在2种基因型(++型、CC型). 相似文献
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为挖掘脊椎动物胚胎发育过程中皮肤调控模式的改变,以鸡胚作为模型,利用来源于NCBI数据库的SRA数据子库中胚胎发育第6~21天的皮肤组织RNA-Seq数据进行聚类分析,在基因共表达网络、信号通路、蛋白互作网路3个层面基于网络拓扑属性逐步挖掘核心基因集,并分析核心基因集在不同发育阶段的互作网络变化。结果表明:1)鸡胚皮肤发育过程在基因谱水平上可分成5个阶段,分别为6~9 d、10~12 d、13~14 d、15~17 d和18~21 d;2)功能富集分析表明发育早期的皮肤分化程度低、可塑性强,中期是皮肤毛囊发育关键期,最后4 d为皮肤细胞外基质建立期,分子发育过程存在严格的顺序模式;3)在基因调控网络层面深度挖掘并筛选鸡胚皮肤发育的分子调控过程。综上,本研究根据网路拓扑属性结合蛋白互作数据库提出参与不同发育模块的关键枢纽基因,为转录组测序数据的分析及脊椎动物皮肤发育过程的分子机制研究提供一些新的视角和分析方法。 相似文献
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