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Aquaculture International - The utilization of sustainable and innovative raw materials to substitute for traditional fishmeal (FM) ingredients is required for the aquaculture sector. Sacha inchi...  相似文献   
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In this study, RAPD and ISSR markers were used to investigate the genetic diversity and genetic relationships among different germplasm of Nelumbo including 70 Chinese ornamental cultivars, 7 wild Thai genotypes, 2 Nelumbo lutea genotypes and 8 hybrids of Nelumbo nucifera and N. lutea. High genetic diversities of 96.4% and 91.2% respectively were detected in the Nelumbo accessions using RAPD and ISSR markers. A dendrogram based on both RAPD and ISSR clustering data indicated that: (1) the genotypes of N. nucifera and N. lutea from different geographical origins were clustered into different groups. This indicated significant genetic differentiation attributed to extensive periods of geographical isolation and lack of gene exchange; (2) the Thai wild genotypes were separated from Chinese genotypes. This indicated genetic divergence between germplasm from Southeast Asia and that from China. Geographical location appears to have affected genetic diversity due to adaptation of the plants to the different environments. A new Southeastern Asia Lotus category is suggested as an addition to the current lotus cultivars classification system; (3) data on three morphological traits (namely: plant size, petal shape and flower color), showed that only the data on plant size was consistent with the dendrogram constructed from molecular data. This finding suggests that using data on genetic relationships in combination with morphological characteristics would serve to improve the classification system of lotus cultivars currently in use. The finding of previously unknown germplasm in this study indicated the potential of RAPD and ISSR techniques in identifying and managing lotus resources. Both marker techniques are potentially useful in improving the current strategies in breeding and germplasm conservation to enhance the ornamental and economic value of lotus.  相似文献   
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借助生物信息学工具对辣椒热激蛋白HSP90家族基因进行鉴定,并分析其理化性质、结构特征、系统进化关系以及在各组织器官和高温胁迫下的表达模式。结果表明,辣椒基因组中含有7个HSP90家族基因,分布于5条染色体上,内含子数2 ~ 19不等,含有5个保守基序;系统进化关系分析显示辣椒7个HSP90家族基因分为3组;亚细胞定位结果显示辣椒HSP90蛋白定位于细胞质与内质网中;基于已发表的转录组数据进行组织表达分析,HSP90在不同组织中的表达模式不同;高温处理可不同程度地激活HSP90的表达。  相似文献   
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