母猪卧向行为学的观察     

邓红雨  范佳英  包军 《家畜生态》2005,(5)

本文通过2个试验对母猪在分娩圈和分娩栏中的卧向行为进行了观察,试验一中, 选择10头大白(Yorkshire)经产母猪作为观察对象,试验二中分别选择10头大白(Yorkshire) 经产母猪和10头长白(Landrace)经产母猪作为观察对象。观察采用瞬时记录方法,每周观察 三次,隔日观察,每天上午、下午各观察一次,每次3 h,每次观察间隔5 min。观察中发现,母猪 卧向以向外为主。分娩栏(四周是栏杆)中的母猪以选择卧向外(向北)为主,其次是卧向内(向 南),卧向左和卧向右最少且差异不显著,上栏前和下栏后差异不显著。分娩圈(四周是墙壁)中 母猪以选择卧向外为主,卧向内最少,卧向左和卧向右差异不显著。长白母猪比大白母猪选择 卧向外的多,妊娠阶段比哺乳阶段选择卧向外的多。  相似文献   

母猪卧向行为学的观察     

邓红雨  范佳英  包军 《家畜生态学报》2005,26(5):52-54

本文通过2个试验对母猪在分娩圈和分娩栏中的卧向行为进行了观察，试验一中，选择10头大白（Yorkshire）经产母猪作为观察对象，试验二中分别选择10头大白（Yorkshire）经产母猪和10头长白（Landrace）经产母猪作为观察对象。观察采用瞬时记录方法，每周观察三次，隔日观察，每天上午、下午各观察一次，每次3h，每次观察间隔5min。观察中发现，母猪卧向以向外为主。分娩栏（四周是栏杆）中的母猪以选择卧向外（向北）为主，其次是卧向内（向南），卧向左和卧向右最少且差异不显著，上栏前和下栏后差异不显著。分娩圈（四周是墙壁）中母猪以选择卧向外为主，卧向内最少，卧向左和卧向右差异不显著。长白母猪比大白母猪选择卧向外的多，妊娠阶段比哺乳阶段选择卧向外的多。  相似文献   

A new Curvularia lunata variety discovered in Huanghuaihai Region in China          下载免费PDF全文

CHANG Jia-ying  LIU Shu-sen  SHI Jie  GUO Ning  ZHANG Hai-jian  CHEN Jie 《农业科学学报》2020,19(2):551-560

The purpose of this study was to identify the dominant pathogens of Curvularia leaf spot and their pathogenicity variation in Huanghuaihai Region of China in recent years. In 2013 and 2016–2017, the occurrences of Curvularia leaf spots on maize were investigated in fields located in Henan, Hebei, Shandong, and Anhui provinces, and 292 fungi were isolated from diseased leaves. These fungal isolates were subjected to morphological identification, and 232 isolates were found to have about 70% uncurved conidia and were identified as Curvularia lunata var. Most of the conidia of 2 representative isolates, namely, HNWB-131 and HNWB-185, were oblong with parallel septations and were distinctly different from a reference isolate CX-3. For further determination, the internal transcribed spacer(ITS), glyceraldehyde 3-phosphate dehydrogenase(GPDH), the large subunit(LSU), and translation elongation factor 1-alpha(EF1-α) sequences of HNWB-131, HNWB-185, and CX-3 were amplified and sequenced. The results of sequence analysis showed that the 4 gene sequences from the 3 isolates had a similarity of more than 99% to C. lunata. Based on the sequences of ITS and the combined data of the 4 genes, neighbor-joining trees were constructed for phylogenetic analysis. The results indicated that these 3 isolates were clustered together with C. lunata. The expression of Clg2 p and ClUrase genes in mycelia and conidia was significantly(P0.05) higher in CX-3 than in HNWB-131 and HNWB-185. This study found that the dominant pathogen of Curvularia leaf spot was a new variety of C. lunata with morphological variations in Huanghuaihai Region from 2013 to 2017. The pathogenicity of the C. lunata var. was not significantly enhanced, and the expression of Clg2 p and ClUrase genes of C. lunata var. was decreased.  相似文献   

不同土壤条件下施用硝化抑制剂DMPG对葡萄生长和品质的影响          下载免费PDF全文

叶英新  张文静  马宾  张登晓  朱佳颖  王术  赵龙  王璐 《中国土壤与肥料》2023,(2):113-121

为探究在不同土壤条件下新型硝化抑制剂3,4-二甲基吡唑磷酸盐(DMPG)对鲜食葡萄生长的影响,以西昌克伦生和秦皇岛玫瑰香2个葡萄品种为研究对象,在田间试验条件下采用多点试验方法,研究DMPG对土壤养分、葡萄生长性状和品质的影响。结果表明：配施0.35%DMPG对土壤铵态氮和硝态氮含量均有显著影响,较CK处理土壤NH₄⁺-N含量增加17.58%～26.06%,NO₃^--N含量降低11.72%～27.42%;但对土壤pH、有效磷和速效钾无明显影响。DMPG处理对克伦生和玫瑰香叶绿素含量、叶面积和叶片厚度均有促进作用,与CK相比,克伦生和玫瑰香叶面积平均分别增加11.32%和9.85%,叶片厚度平均分别增加9.05%和16.22%。同时,DMPG处理可提高葡萄果实纵横径、百粒重和可溶性固形物含量,西昌克伦生3个试验点果实纵径、横径分别显著增加7.2%～11.04%、7.10%～9.82%,百粒重平均提高29.14%,而秦皇岛玫瑰香效果不显著。因此,在葡萄种植中施用DMPG能够调控土壤氮素养分,改善土壤肥力...  相似文献   

Essential role of Pax-8 gene during embryonic heart development     

ZHANG Jia-ying  LAI Dan-dan  ZHU Mao-ping  WANG Sai-bin  SHI Xiang-xiang  NI Qiu-ming  HUANG Xiao-yan  ZHANG Huai-qin  YANG De-ye 《园艺学报》2009,25(7):1292-1297

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氯化三苯基锡对鲫的急性和亚急性毒性研究     

韩照祥  李佳颖  孙涛 《水生态学杂志》2008,29(2):62-66

以3月龄的鲫( Carassius auratus ) 为实验对象, 研究了氯化三苯基锡( TPTC) 对鲫的急性毒性以及在1 /2LD50、1 /3LD50、1 /4LD50、1 /6LD50亚急性浓度下对鲫死亡率的影响。结果表明, 在较低的TPTC 浓度下, 鲫的概率单位较小, 死亡率较低; 当浓度达到385. 6 Lg /L时, 死亡率超过50%。因此, TPTC 浓度385 Lg /L是一个安全阈值, 当TPTC 浓度达到385 Lg /L以上, 死亡率明显增加。TPTC的对数浓度和概率单位呈现出明显的线性相关; 且TPTC在鲫体内的积累量较大, 难以降解; 鲫对TPTC比较敏感, 可将其作为TPTC毒性测试的标准试验生物。  相似文献   

Effects of ZIP14 on biological behaviors of hepatocellular carcinoma cell line BEL-7404     

ZHENG Jia-ying  LI Ya-dong  ZHENG Qing-zhu  YU Li-li  WU Qing-wei  QIU Fu-nan  WU Yan-an  HUANG Yi 《园艺学报》2019,35(5):804-812

AIM: To study the expression of zinc transporter ZRT/IRT-like protein 14 (ZIP14) in the hepatocellular carcinoma (HCC) tissues, and to investigate the effects of ZIP14 over-expression on the biological behaviors of HCC cells. METHODS: The expression of ZIP14 at mRNA and protein levels in the HCC tissues and adjacent non-tumor tissues were detected by real-time PCR and immunohistochemical staining, respectively. The lentivirus expression system containing GV365-ZIP14 was constructed, and was used to infect the HCC cell line BEL-7404, which had relatively poor expression of ZIP14. The expression of ZIP14 at mRNA and protein levels in the transfected cells were detected by real-time PCR and Western blot, respectively. Under the conditions of zinc sulfate stimulation at different concentrations, the cell viability, the cell cycle, and the cell migration and invasion abilities were detected by MTT assay, DNA ploid detection, and Transwell assay, respectively. RESULTS: The mRNA expression level and the strong-positive rate of protein expression of ZIP14 in the HCC tissues were significantly lower than those in the adjacent non-tumor liver tissues (P<0.01). The expression of ZIP14 at mRNA and protein levels in the BEL7404 cells was significantly enhanced by infection of GV365-ZIP14 expression lentivirus. Compared with negative control group (transfected with negative control lentivirus), the cell viability, migration and invasion in ZIP14 over-expression group (transfected with GV365-ZIP14 expression lentivirus) were significantly reduced, and the percentage of the cells in G₂/M phase was significantly increased, all of which were more obvious with the elevation of zinc concentration in the culture medium. CONCLUSION: ZIP14 is low expressed in the HCC tissues. The ZIP14 over-expression has inhibitory effects on the viability, migration and invasion of HCC cells, and blocks the cell cycle in G₂/M phase, which might be closely related to the elevation of zinc concentration in cytoplasma of HCC cells due to enchanced zinc transport by ZIP14.  相似文献   

如何解决农民就地城市化的问题研究     

熊佳颖 《湖南农机》2017,44(6)

当前,农民就地城市化已经成为了一个重要的问题,文章主要分析了如何进一步解决农民就地城市化的问题,针对这些问题进行了探讨和分析,提出了进一步的对策和建议,希望能够为今后农民就地城市化的问题提供更好的参考和借鉴.  相似文献   

中性纤维素酶整理牛仔布的工艺优化   总被引：1，自引：0，他引：1  

蔡金涛  焦聪  崔琰  杨佳颖  陈红漫  阚国仕 《辽宁农业科学》2011,(3):12-15

根据芽孢杆菌M01-ZL-110所产纤维素酶的酶系组成及酶学性质特点,将该菌株发酵所产纤维素酶酶液应用于牛仔布的整理工艺。以减量率为试验评价指标,通过正交试验对加酶量、温度、整理时间及pH值等参数进行优化。试验结果表明由芽孢杆菌M01-ZL-110制备的中性纤维素酶对牛仔布酶洗整理的最佳条件为：30 m l的溶液体系中酶液加入量为20 m l,pH为6,温度为60℃,整理时间是60 m in,并且对牛仔布表面进行了酶洗前后扫描电镜观察比较。  相似文献   

盐胁迫下不同粳稻品种的形态和生理特性   总被引：1，自引：0，他引：1  

田蕾  王娜  张雪艳  杨斌林  孙佳莹  李敏  李培富 《广东农业科学》2014,41(23):1-6

用国际水稻所标准营养液培养60个粳稻品种,发芽14 d后加入125 mmol/L NaCl胁迫处理,于盐胁迫后第3、6d分别取样测定叶片伤害率、叶片丙二醛含量;叶片盐害症状明显时(盐胁迫6d后),根据盐害症状评价水稻耐盐级别,分别测定株高、地上部和根的干鲜重等耐盐相关生理指标.结果表明,叶片伤害率、叶片丙二醛含量等生理指标均与耐盐级别呈极显著负相关,耐盐品种的相对干鲜重极显著高于盐敏感品种,而叶片伤害率、盐胁迫6d后叶片丙二醛含量则均极显著低于盐敏感品种;利用耐盐级别将各品种分为耐盐组(T)、中间组(M)和盐敏感组(S)3组,3组品种盐胁迫3d叶片丙二醛含量差异不显著,而耐盐组(T)的叶片伤害率(盐处理3、6d),盐胁迫6d叶片丙二醛含量均极显著低于盐敏感组(S)和中间组(M),且在分布范围上与盐敏感组没有重叠部分.表明这两个指标能有效区分苗期耐盐和盐敏感水稻品种,可作为定量生理指标评价水稻苗期耐盐性,为水稻种质资源苗期耐盐鉴定,耐盐基因发掘和品种培育奠定了基础.  相似文献