Detection of hemorrhagic septicemia virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two noncompetitive monoclonal antibodies against early viral nucleoproteins.     

F A Sanz  J M Coll 《American journal of veterinary research》1992,53(6):897-903

Inclusion of high-ionic strength buffers helped us to develop a sandwich ELISA to detect hemorrhagic septicemia virus (HSV) in cell culture and infected trout tissue extracts. For maximal sensitivity of 0.1 to 0.2 ng/well/100 microliters or about 10 to 50 TCID50/well/100 microliters, trout extracts were diluted 1:1 and assayed for the earliest synthesized nucleoprotein N. Simultaneous binding of the N protein from HSV in the sample to the wells coated with monoclonal antibody (2D5 against the N protein) and to the peroxidase-labeled monoclonal antibody (2C9 against the N protein) proceeded during a 2-hour incubation at 20 to 22 C (room temperature). The response was linear between 6 to 60 ng/well of purified virus. Monoclonal antibodies used were noncompetitive with each other and reacted with F1, F2, 23.75, and 5 Spanish isolates of HSV, but not with infectious hematopoietic necrosis or infectious pancreatic necrosis viruses. Tissue specimens with low content of HSV virus may now be assayed directly without use of cell culture, rapidly, and with high precision, during the acute phase of the disease in salmonid fishes.  相似文献   

作物活体多探头测量仪的研制     

罗时石  龚荐  葛才林  张浩  彭永欣  封超年  郭文善 《扬州大学学报(农业与生命科学版)》1991,(4)

采用TP—801单板机制作作物活体多探头测量仪。该机可在田间对生长状态下的农作物进行多点体内养分积累、运输、分配等测定,且结果可靠。  相似文献   

江苏毛竹林生产力类型区的划分及分析   总被引：1，自引：0，他引：1  

卢义山  徐宜良 《江苏林业科技》1992,19(3):1-5

根据毛竹生产力和环境指标将江苏省毛竹林区划分出高生产力、中生产力、一般生产力和低生产力类型区,并分析了各类型区的主要特点。同时,提出了各类型区的经营发展方向。  相似文献   

苏北滨海盐土区杉木生态适应性及栽培技术的研究     

王为耘  丁神奇 《江苏林业科技》1996,23(3):32-35

调查研究结果表明，盐城市滨海盐土区与杉木中心产区的气候因子比较接近，基本适宜杉木的生长，杉木造林选择地下一在１．５ｍ以下，排水良好的采伐更新迹地并采用 栽植法效果较好。新造幼林套种作物以棉花最好。  相似文献   

A Quantitative Polymerase Chain Reaction Assay for the Detection and Quantification of Epizootic Epitheliotropic Disease Virus (EEDV; Salmonid Herpesvirus 3)     

Gavin W. Glenney  Patricia A. Barbash  John A. Coll 《Journal of aquatic animal health》2016,28(1):56-67

Epizootic epitheliotropic disease virus (EEDV; salmonid herpesvirus [SalHV3]; family Alloherpesviridae) causes a systemic disease of juvenile and yearling Lake Trout Salvelinus namaycush. No cell lines are currently available for the culture and propagation of EEDV, so primary diagnosis is limited to PCR and electron microscopy. To better understand the pervasiveness of EEDV (carrier or latent state of infection) in domesticated and wild Lake Trout populations, we developed a sensitive TaqMan quantitative PCR (qPCR) assay to detect the presence of the EEDV terminase gene in Lake Trout tissues. This assay was able to detect a linear standard curve over nine logs of plasmid dilution and was sensitive enough to detect single-digit copies of EEDV. The efficiency of the PCR assay was 99.4 ± 0.06% (mean ± SD), with a 95% confidence limit of 0.0296 (R² = 0.994). Methods were successfully applied to collect preliminary data from a number of species and water bodies in the states of Pennsylvania, New York, and Vermont, indicating that EEDV is more common in wild fish than previously known. In addition, through the development of this qPCR assay, we detected EEDV in a new salmonid species, the Cisco Coregonus artedi. The qPCR assay was unexpectedly able to detect two additional herpesviruses, the Atlantic Salmon papillomatosis virus (ASPV; SalHV4) and the Namaycush herpesvirus (NamHV; SalHV5), which both share high sequence identity with the EEDV terminase gene. With these unexpected findings, we subsequently designed three primer sets to confirm initial TaqMan qPCR assay positives and to differentiate among EEDV, ASPV, and NamHV by detecting the glycoprotein genes via SYBR Green qPCR.

Received April 20, 2015; accepted November 10, 2015  相似文献   

棉花烂铃空间格局及抽样技术研究     

王昆  顾芳明  戴启洲  顾宝贵  周其玉  孙同勋  戴启红  施秀兰  严发根  陈宝兰  贺云梅  李华  谢金莲 《棉花学报》1996,(5)

本文通过聚集度指标（如：Ｃ、Ｋ、Ｃ＿Ａ、Ｉ、Ｉ＿δ、）测定，以及Ｉｗａｏ’线性回归、Ｔａｙｌｏｒ’ｓＳ￣２一回归和格局纹理分析，结果表明，棉花烂铃空间格而在一切密度下都呈聚集型，且聚集强度随烂铃密度的升高而增加，其空间格局的基本成分是疏松的个体群，其大小约为２～５株棉花，平均约含２．８４个烂铃个体，且个体群内的个体呈随机分布，经μ检验和方差分析，双对角线５点式、棋盘式和平行跳跃式等３种取样方法均适用于棉花烂铃田间调查，尤以双对角线５点式为佳，用双对角线５点式应样法调查的最适抽样数：一般病情普查至少１０个样方（每样方系５株棉花），系统测报和科研调查为２０～３０个样方。最后给出了棉花烂铃平均密度的零频率简易估计模型。  相似文献   

特种优质水稻灌溉技术的试验研究     

王永林  胡新民  顾正明 《中国农村水利水电》1993,(9)

为了达到节水、改土和增产的目的,通过研究灌溉方式对耗水量、灌溉定额、灌水次数和特种优质水稻生长发育及其产量的影响,水文年型和品种对耗水量的影响,优选出了特种优质水稻最适宜的田间水分控制模式。  相似文献   

灌区评估指标的选取及灰关联分析     

葛书龙 《中国农村水利水电》1993,(4)

该文探讨了灌区评估指标体系的建立方法,对评估指标的选取与预处理进行了系统分析,并尝试应用灰色系统理论中的灰关联度分析法,对灌区评估指标进行关联分析,从而很方便地进行灌区之间的评估、排队及比较,与传统的强制打分法相比,此法简便易行,无需预先建立评判标准,因此是一种新的评估分析法。  相似文献   

太湖平原水田区灌溉回归水分析     

聂杰 《中国农村水利水电》1992,(2)

江苏太湖平原地区灌溉水源丰富,排水通畅,但由于年际水量分配不均,干旱仍时有发生。根据这一情况,对灌溉水的利用问题进行了研究。在大、小区水量平衡的基础上,提出了灌溉水的回归系数和回归水的重复利用系数,以及灌溉水的重复利用系数。为本区水利规划、用水管理和供水规划提供了可靠的依据。  相似文献   

One-step purification of the major rainbow trout immunoglobulin     

C Sánchez  J Coll  J Domínguez 《Veterinary immunology and immunopathology》1991,27(4):383-391

Salmonid immunoglobulin purification has been hampered by time-consuming, labour-intensive, conventional chromatographic procedures. In this report we describe the use of immunoaffinity chromatography for the purification of immunoglobulins from trout serum in a single step. An anti-heavy chain monoclonal antibody (1G7) which reacts with more than 85% of total trout immunoglobulins was used to purify the trout immunoglobulin. The purity achieved was higher than 95%, and the immunoglobulins recovered were fully immunogenic. This method served equally well in isolating immunoglobulins from the sera of other salmonids (coho salmon and chinook salmon). The procedure should be useful in the standardization of salmonid immunoglobulin reagents.  相似文献