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Studies were carried out to optimize production of nucleopolyhedrovirus of American bollworm,Helicoverpa armigera (Hübner), by treating larvae individually with an inoculum dose that allowed maximal larval growth and also gave the highest
occlusion bodies (OB) yield/larva. The maximum virus yield of 12.2x 109 OB/larva was obtained when 6-day-old larvae were fed individually with a dose of 1 x 103 OB. Topical spiracular treatment of larvae as old as 8 days with 10 μ of 2x 107 OB ml-1 gave the highest yield, of 15.2x 109 OB from 13-day-old larvae, of 12.8x 108 OB from prepupae and of 1.49x 108 OB from pupae at the time of their death. These studies showed that dietary inoculum is the best route for 6-day-old larvae
and topical spiracular treatment is the best for 8-day-old larvae. 相似文献
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S. K. Malik R. Chaudhury O. P. Dhariwal Rajwant K. Kalia 《Genetic Resources and Crop Evolution》2006,53(7):1485-1493
Citrus indica and C. macroptera are the wild endangered species of Citrus occurring in northeastern India. Surveys were undertaken in this region for ascertaining distribution, studying variability
and for collection of germplasm of these two species. C. indica, an endemic species of this region, was collected from the Citrus Gene Sanctuary located in buffer zone of Nokrek Biosphere
Reserve in the Garo hills of Meghalaya. In addition, a putative natural hybrid of C. indica and C. limon was collected for the first time from the south Garo hills. C. macroptera had much wider distribution and was collected from Mizoram and Meghalaya states. In Jantia hills of Meghalaya, natural populations
of this species are in a highly threatened state. The two species were unevenly distributed all over the explored territory.
Morphological characterization of leaves, fruits and seeds indicated the presence of sizable variability within collected
accessions of these two Citrus species. Indigenous technical knowledge gathered on the use and socio-economic importance indicated commercial potential
for these two species in northeastern India. However, lack of cultivation of these species and clearing of forest cover at
an alarming rate has led to an urgent need to adopt complementary conservation strategies to safeguard these species and to
ensure their availability for future utilization. A major emphasis on developing methods for their propagation, multiplication
and regeneration in in situ and ex situ conditions is required. 相似文献
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Feeding and casting activity of Amynthas alexandri fed on corn, wheat leaves, and mixed grasses were monitored in laboratory cultures. Casts were produced on the surface and sides of the containers. Food consumption varied from 36.5 to 69 mg g–1 live worm day–1. Cast production ranged from 3.95 to 5.9 mg g–1 live worm day–1. The C:N ratio in casts in laboratory cultures (11.17) and in field samples (8.84) was consistently lower than the corresponding ratio in the parent soil (13.19 and 10.54, respectively). This was probably due to mineralization of plant-derived organic material during passage through earthworms with consequent low C:N ratios. 相似文献
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Kalia K Sharma K Singh HP Singh B 《Journal of agricultural and food chemistry》2008,56(21):10129-10134
The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential (FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to 41.43) mg of Trolox/g] with ABTS (*+), DPPH (*) and FRAP assays, respectively. In general, TEAC of Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly (1.2 times) higher than microwave. A positive correlation (r(2) = 0.931 to 0.982) was observed between total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity. In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also quantified in different extracts by RP-HPLC. 相似文献
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P. Saha P. Kalia P. Sharma T. R. Sharma 《The Journal of Horticultural Science and Biotechnology》2013,88(5):480-486
SummaryIn order to understand the genetics of resistance to black rot disease caused by Xanthomonas campestris pv. campestris (Xcc) (Pammel) Dowson in cauliflower (Brassica oleracea var. botrytis L.) and to identify random amplified polymorphic DNA (RAPD) markers that segregate with the resistance genes, susceptible (‘Pusa Himjyoti’, female parent) and resistant (‘BR-161’, pollen parent) plants were crossed. Six generations of plants (30 P1, 30 P2,30 F1, 120 F2, 90 B1, and 90 B2) were evaluated for the presence or absence of black rot disease in a randomised block design with three replications. The pattern of segregation of resistance was tested by the χ2 test at the 5% level of significance. All F1 progeny plants were resistant, and the segregation of resistant and susceptible plants in the F2 and two backcross generations (B1 and B2) showed that a single dominant gene caused resistance to the black rot pathogen in ‘BR-161’. Three polymorphic RAPD markers (OPO-04833, OPAW-202538, and OPG-25625) were found by bulk segregant analysis, which produced unique amplicons 833 bp, 2,538 bp, and 625 bp in length, respectively. These markers were associated in coupling phase to the resistance allele. Best fit ratios of 3:1 (resistant:susceptible) in the F2 plants with the three RAPD markers, suggested that the markers were linked to the single gene controlling black rot resistance. These markers will be useful to identify more closely-linked markers and to develop black rot-resistant hybrid cauliflower varieties. 相似文献
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Chromosomal aberrations induced in barley by LSD 总被引：1，自引：0，他引：1
Seeds of hulled barley (Hordeum vulgare) were germinated and then treated with LSD. Preparations of squashed root tips stained with Feulgen revealed extensive chromosomal aberrations, most of which were chromosome breaks. Nearly half of the breaks occurred in the region of the primary constriction. 相似文献