绿色富硒生物猪饲料中AFB₁达标生产工艺优化研究     

郑威  卢婵  余侃  黄思思  余锦平  刘金龙  程水源  魏金涛  李良华  刘欋霄  郑倬  田国政  周光来 《中国饲料》2021,(5):84-90

饲料中黄曲霉毒素(AFB₁)容易超标,检出率达80%~100%,毒性大,具强致癌性,可抑制生猪免疫机能,降低动物生产性能,引起动物继发感染,还会在动物产品中残留而威胁人类健康,给生猪养殖带来经济损失,降低猪肉食品安全性。本文通过使用先进固体发酵系统设备和益生菌发酵技术,采用单因素试验和响应面中试优化,获得发酵降解猪饲料AFB₁的最佳工艺参数为硒浓度0.3 mg/kg,发酵时间12 h,量子波强度30 Hz,益生菌菌种组合CGMCC NO.17328混合CGMCC NO.15611。该工艺将猪饲料AFB₁量从63.41μg/kg降解到2.98μg/kg,降解率达到95.30%,AFB₁含量达到国家饲料安全标准。生产工艺适合养猪场低成本快速生产AFB₁达标猪饲料。  相似文献   

核桃扁叶甲幼虫龄数及取食量研究     

刘童童  邱建生 《安徽农学通报》2021,27(20):95-97,104

为明确核桃扁叶甲幼虫的发育状况,以便进行预测预报及采取防治措施,通过测量不同时间段核桃扁叶甲幼虫的体长、体宽、头宽和角长等4项形态指标,运用频数分布法和Crosby生长法推断核桃扁叶甲幼虫的龄数,并对其发育中各个龄期幼虫的取食量进行了测定.结果表明:核桃扁叶甲幼虫共有3龄,头宽是最佳的幼虫分龄指标,体长、体宽和角长则不适合作为分龄指标;幼虫取食量随龄期的增大而增加,且增加十分明显;幼虫取食造成危害的主要阶段为3龄幼虫期,日平均取食量随天数的增加而增大,最高峰是在孵化后的第12天.  相似文献   

微机综合自动化系统在大型泵站中的应用   总被引：8，自引：3，他引：5  

阚永庚  雍成林 《中国农村水利水电》2001,(3):40-42

水利部“94．8”项目之一“江都抽水机站机组监控关键技术”的实施，在国内首次实现了大型泵站“无人值守，少人值班”的新局面，该系统自动化调节功能，充分利用潮汐河流的特点，实现了泵站的优化调度，提高了运行效率，挖掘了泵站的潜在抽水能力，不仅节约了运行费用，而且还能增灌农田，显著地提高了经济效益和社会效益。  相似文献   

吉林省西部新开发水田遥感动态监测研究     

扈晶晶  李瑞平  黄侃  黄华国 《林业调查规划》2014,(3):17-23

为了响应土地整理开发项目的政策,吉林省西部地区开发了大面积的水田。为掌握新开发水田的动态变化,有必要对吉林省地区进行土地利用分类。以吉林省前郭县为例,利用2010年环境卫星数据进行不同土地利用分类方法的比较,进而对另外3景图像进行信息提取,分析4个年份新开发水田的分布及变化情况。结果表明,支持向量机法比最大似然法的耕地分类精度高约5%,其产品和用户精度分别为95%和84%。加入纹理信息没有显著提高分类精度。2009~2012年水田面积分别增加-67.7 km2,111.7 km2和265.01 km2。  相似文献   

烟曲霉素类药物在农业领域的应用研究进展     

常白杨  王彤彤  汪建明  王敏 《中国农业科技导报》2017,19(9):57-62

烟曲霉素是一种从烟曲霉菌(Aspergillus fumigatus)提取液中分离出来的霉菌毒素,其在防治微孢子虫病方面有显著疗效,在农业中广泛作为抗生素类药物用于治疗此病。综述了烟曲霉素的起源、分子结构和化学性质,烟曲霉素类药物在国内外的研究现状,以及烟曲霉素类药物在蜜蜂、鱼类等农业领域的应用;并对合成低毒的烟曲霉素类药物、合成烟曲霉素类似物或代谢物的相关研究及其在农业中的应用前景进行了展望;旨在为烟曲霉素相关药物在我国农业领域的应用提供一些帮助,以及为今后合成一种低毒、高效的烟曲霉素药物提供理论依据。  相似文献   

Association analysis for detecting significant single nucleotide polymorphisms for phosphorus-deficiency tolerance at the seedling stage in soybean [Glycine max (L) Merr.]     

Lihua Ning  Guizhen Kan  Wenkai Du  Shiwei Guo  Qing Wang  Guozheng Zhang  Hao Cheng  Deyue Yu 《Breeding Science》2016,66(2):191-203

Tolerance to low-phosphorus soil is a desirable trait in soybean cultivars. Previous quantitative trait locus (QTL) studies for phosphorus-deficiency tolerance were mainly derived from bi-parental segregating populations and few reports from natural population. The objective of this study was to detect QTLs that regulate phosphorus-deficiency tolerance in soybean using association mapping approach. Phosphorus-deficiency tolerance was evaluated according to five traits (plant shoot height, shoot dry weight, phosphorus concentration, phosphorus acquisition efficiency and use efficiency) comprising a conditional phenotype at the seedling stage. Association mapping of the conditional phenotype detected 19 SNPs including 13 SNPs that were significantly associated with the five traits across two years. A novel cluster of SNPs, including three SNPs that consistently showed significant effects over two years, that associated with more than one trait was detected on chromosome 3. All favorable alleles, which were determined based on the mean of conditional phenotypic values of each trait over the two years, could be pyramided into one cultivar through parental cross combination. The best three cross combinations were predicted with the aim of simultaneously improving phosphorus acquisition efficiency and use efficiency. These results will provide a thorough understanding of the genetic basis of phosphorus deficiency tolerance in soybean.  相似文献   

高浓度葡萄糖对猪卵母细胞体外成熟和早期胚胎发育的影响     

张童桐  司莉南  许红霞  程文秀  陶瑞鑫  李娟 《畜牧与兽医》2021,(4):10-16

试验旨在探究高浓度葡萄糖对猪卵母细胞体外成熟及早期胚胎发育能力的影响。取体外分离处于生发泡期的猪卵丘卵母细胞复合体(COCs),分为3个处理组。分别用含葡萄糖浓度为5.6 mmol/L(C组)、10 mmol/L(G-1组)、15 mmol/L(G-2组)的培养液,进行体外成熟(IVM)处理,42 h后观察,并统计卵丘细胞扩散情况和第一极体排出率;对体外成熟42 h后的卵母细胞孤雌激活,统计2-细胞、4-细胞和第7天囊胚发育。结果发现,G-1组和G-2组卵丘细胞扩散度显著低于C组(P<0.05);G-1组和G-2组的MII期卵母细胞死亡率和存活率与C组相比无显著差异(P>0.05),但G-1组极体率显著降低(P<0.05),G-2组极体率极显著低于C组(P<0.01)。孤雌激活后,与C组相比,G-1组和G-2组的2-细胞分裂率显著降低(P<0.05),4-细胞分裂率以及囊胚发育率均极显著降低(P<0.01),但G-1、G-2组囊胚细胞数量与C组相比无显著性差异(P>0.05)。进一步线粒体染色发现,G-1组和G-2组的线粒体与C组相比分布不均。与C组相比,荧光结果显示G-1组和G-2组不仅活性氧(ROS)水平极显著升高(P<0.01),而且G-1组与G-2组谷胱甘肽(GSH)含量显著降低(P<0.05),虽然G-1组丙二醛(MDA)无显著性差异(P>0.05),但G-2组丙二醛(MDA)含量显著升高(P<0.05)。研究结果表明,葡萄糖浓度高会影响猪卵母细胞线粒体分布,氧化应激水平升高,成熟效率降低,损害早期胚胎的发育潜能。  相似文献   

Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells     

Yukako Tokutake  Marcin Taciak  Kan Sato  Masaaki Toyomizu  Motoi Kikusato 《Animal Science Journal》2021,92(1):e13604

Peptide transporter 1 (PepT1) is a transporter responsible for absorbing dipeptide and tripeptide in enterocytes and is upregulated by dipeptide in mammals. It has not been certain whether intestinal PepT1 expression is responsive to dipeptides in chickens because of the lack of in vitro study using the cultured enterocytes. This study established a primary culture model of chicken intestinal epithelial cells (IECs) in two-dimensional monolayer culture using collagen gel by which the response of chicken PepT1 gene expression to dipeptide stimuli was evaluated. The cultured chicken IECs showed the epithelial-like morphology attached in a patch-manner and exhibited positive expression of cytokeratin and epithelial cadherin, specific marker proteins of epithelial cells. Moreover, the chicken IECs exhibited the gene expression of intestinal cell type-specific marker, villin1, mucin 2, and chromogranin A, suggesting that the cultured IECs were composed of enterocytes as well as goblet and enteroendocrine cells. PepT1 gene expression was significantly upregulated by synthetic dipeptide, glycyl-l-glutamine, in the cultured IECs. From the results, we herein suggested that dipeptide is a factor upregulating PepT1 gene expression in chicken IECs.  相似文献   

香蕉茎秆纤维提取机刀片的分析和优化设计—基于SolidWorks     

李成斌  梁栋  张喜瑞  郑侃  陈实 《农机化研究》2015,(1):68-70,74

为减小香蕉茎秆纤维提取机刀片工作时的最大应力,增大其安全系数,利用SolidWorks软件对刀片进行三维建模,运用软件的Simulation插件分析刀片的应力、应变云图,并对刀片相关尺寸进行优化设计。优化结果显示,刀片的最大应力由68.58MPa下降到49.33MPa,下降约28%;刀片的最小安全系数由3.21上升到4.47,增加了约1.4倍;刀片的质量由0.702kg下降到0.698kg,达到了优化的目标。  相似文献   

基于模糊层次分析法的农产品加工企业风险评价研究   总被引：1，自引：0，他引：1  

王侃 《中国农机化》2006,(5):76-79

风险评价是风险管理的重要阶段,针对农产品加工企业特点,提出了一种基于模糊层次分析法的农产品加工企业风险评价方法。该方法初步解决了农产品加工企业风险管理中的关键问题——风险因素的识别。以某农产品加工公司为例,介绍了该方法的应用过程。  相似文献