Laboratory diagnosis and disease occurrence in the current African swine fever eradication program in Spain, 1989–1991     

Steen Bech-Nielsen  Maria Louisa Arias  Jesus Panadero  Jose M. Escribano  Concepcion Gomez-Tejedor  Q. Perez Bonilla  J. M. Sanchez-Vizcaino 《Preventive veterinary medicine》1993,17(3-4):225-234

The purpose of this study was to determine the rate of decline and geographical distribution by municipality of clinical and subclinical African swine fever (ASF) in the affected areas of Spain. A second aim was to evaluate the performance of diagnostic tests in the Spanish ASF eradication program. Clinical outbreaks were confirmed using both the direct and indirect immunofluorescence test (and if both were negative, by the hemabsorption test). The serological status of swine was determined by an enzyme-linked immunosorbent assay (ELISA) and suspect serum samples were confirmed by the immunoblot assay.

The number of clinical outbreaks (herds) of ASF for 1989, 1990 and 1991 was 170, 347 and 207, respectively. The numbers of municipalities within each affected province experiencing acute outbreaks for the same time periods were 49, 69 and 48, respectively. Serologically diagnosed animals positive for ASF were 1.1% of animals tested in 1989, 0.5% in 1990 and 0.8% in 1991. The corresponding positive predictive values of the standard ELISA test used were 99.0, 97.9 and 98.8, respectively. Similarly, the number of municipalities within each affected province experiencing serologically positive subclinically infected animals was 269, 178 and 147 for each of the years 1989, 1990 and 1991, respectively.  相似文献   

The effect of sowing date, stale seedbed, row width and mechanical weed control on weeds and yields of organic winter wheat   总被引：1，自引：0，他引：1  

I A Rasmussen 《Weed Research》2004,44(1):12-20

Three field experiments were carried out in organically grown winter wheat in Denmark. The treatments were sowing time (normal or late sowing) and false seedbed, row width (12 and 24 cm) and weed control method [untreated; mechanical weed control (weed harrowing at 12 cm supplemented with inter‐row hoeing at 24 cm); and herbicide weed control]. Weed biomass in midsummer was greatest on plots sown at the normal sowing time (compared with delayed sowing) and was reduced by mechanical or chemical weed control (compared with untreated plots). Row width alone had no influence on weed biomass, but in the experiment with high weed pressure, the more intensive mechanical weed control used at a row width of 24 cm reduced weed biomass. Normal sowing time tended to give higher yields, but this was only statistically significant in one of the three experiments. Wide rows gave a yield decrease in the experiment with low weed pressure. The effect of weed control on yield was dependent on the weed pressure. At low weed pressure, mechanical weed control caused a yield decrease compared with untreated or herbicide treated. At intermediate weed levels there were no differences, whereas at high weed pressure, mechanical weed control and herbicide treatment caused a yield increase compared with untreated. False seedbeds were shown to contribute to a decrease in the soil seed reserve.  相似文献   

Skin lesions in Aubrac cows strongly associated with fly bites (Haematobia irritans)          下载免费PDF全文

Marion Mosca  Murielle Vabret  Pia Randleff‐Rasmussen  Didier Pin 《Veterinary dermatology》2018,29(3):254-e94

  相似文献   

Early interspecific dynamics,dry matter production and nitrogen use in Kernza (Thinopyrum intermedium) – alfalfa (Medicago sativa L.) mixed intercropping     

Shoujiao Li  Ana Barreiro  Erik Steen Jensen  Yingjun Zhang 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(2):165-175

ABSTRACT

The global interest in growing perennial grain crops such as intermediate wheatgrass (Thinopyrum intermedium) (Kernza) for production of food and feed is increasing. Intercropping Kernza with legumes may be a sustainable way of supplying nitrogen to soil and associated intercrop. We determined the competitive interactions between intercropped Kernza (K) and alfalfa (Medicago sativa L.) (A) under three inorganic nitrogen (N) rates N0, N1, N2 (0, 200, 400 kg ha^?1) and five species relative frequencies (SRF) (100%K:0%A, 75%K:25%A, 50%K:50%A, 25%K:75%A and 0% K:100%A) in mixed intercrops (MI) in a greenhouse pot experiment. After 11 weeks of growth. Kernza dry matter yield (DM) and N accumulated (NACC) were low, but alfalfa DM and NACC high at 0 kg N ha^?1. 200 and 400 kg N ha^?1 fertiliser application increased the competitive ability (CA) of Kernza and reduced the CA of alfalfa. SRF had large impacts on alfalfa DM, NACC and NFIX only at 0 kg N ha^?1 fertiliser, and insignificant impacts on Kernza at all N fertiliser levels, indicating that adjustment of SRF may not be an effective way to modulate the interspecific competition of Kernza. Further research on the other factors that influence the interspecific competition are warranted.  相似文献   

Fertilizer ammonium : nitrate ratios determine phosphorus uptake by young maize plants     

Ingeborg F. Pedersen  Peter Srensen  Jim Rasmussen  Paul J. A. Withers  Gitte Holton Rubk 《植物养料与土壤学杂志》2019,182(4):541-551

We investigated the interacting effects of inorganic nitrogen and the main inorganic phosphorus form in dairy manure (dicalcium phosphate, CaHPO₄) on growth, nutrient uptake, and rhizosphere pH of young maize plants. In a pot experiment, three levels of CaHPO₄ (0, 167, and 500 mg P pot^?1) were combined with nitrogen (637 mg N pot^?1) applied at five NH₄‐N : NO₃‐N ratios (0 : 100, 25 : 75, 50 : 50, 75 : 25, and 100 : 0) and a nitrification inhibitor in a concentrated layer of a typical acid sandy soil from Denmark. ¹⁵N‐labeled NH₄‐N was applied to differentiate the role of nitrification and to partition nitrogen uptake derived from NH₄‐N. Among treatments including nitrogen, shoot biomass, rooting and phosphorus uptake were significantly higher at the five‐leaf stage when CaHPO₄ was applied with NH₄‐N : NO₃‐N ratios of 50 : 50 and 75 : 25. In these treatments, rhizosphere pH dropped significantly in direct proportion with NH₄‐N uptake. The fertilizers in the concentrated layer had a root‐inhibiting effect in treatments without phosphorus supply and in treatments with pure NO₃‐N or NH₄‐N supply. Increased nitrogen uptake as NH₄‐N instead of NO₃‐N reduced rhizosphere pH and enhanced acquisition of applied CaHPO₄ by young maize plants, which may have positive implications for the enhanced utilization of manure phosphorus.  相似文献   

Serum levels of reproductive steroid hormones in captive sand tiger sharks, <Emphasis Type="Italic">Carcharias</Emphasis><Emphasis Type="Italic">taurus</Emphasis> (Rafinesque), and comments on their relation to sexual conflicts     

Henningsen AD  Murru FL  Rasmussen LE  Whitaker BR  Violetta GC 《Fish physiology and biochemistry》2008,34(4):437-446

Levels of reproductively-related steroids were determined in captive male sand tiger sharks, Carcharias taurus, maintained at two institutions: SeaWorld Adventure Park Orlando and the National Aquarium in Baltimore. Sexual conflicts were absent at the former, but were documented at the latter. Serum titers of 17β-estradiol, progesterone, testosterone, and 5α-dihydrotestosterone were determined via radioimmunoassay in adult male sharks from 1988 to 2000. Sampling overlap between the two institutions occurred for 3 months of the year, but steroid concentrations were compared only for April due to the occurrence of sexual conflicts in the sharks at the National Aquarium in Baltimore in that month. For April, testosterone and dihydrotestosterone were significantly higher in the SeaWorld males, and progesterone was significantly higher in the National Aquarium in Baltimore males, while estradiol was not significantly different. Steroid levels were also determined from serial samples taken monthly over 17 months from three male sharks and one female shark at the National Aquarium in Baltimore in 2001–2002 and were compared with corresponding observed sexual conflicts. The steroid levels obtained showed distinct annual hormonal cycles in the male sharks and corroborated a biennial cycle for the single serially-sampled female shark. Furthermore, the steroid levels for individual males correlated with sexual conflicts as well as their position within the male dominance hierarchy. As this species is depleted in some regions globally, insight into the steroid profile of mature sand tiger sharks is important for a greater understanding of the relationship between their reproductive physiology and behavior, and may aid in captive management and reproduction. L. E. L. Rasmussen—Deceased.  相似文献   

Use of in-growth soil cores in mesh bags for studies of relations between soil compaction and root growth     

Eliel Steen  Inge Hkansson 《Soil & Tillage Research》1987,10(4)

Using in-growth soil cores in cylindrical mesh bags, the effects of 3 soil compaction treatments on growth of crop roots were studied in a sandy soil. The bags were inserted after crop emergence in holes (70 mm diameter; 60 cm depth) augered in the soil in crop row interspaces. In 1984 (with rapessed), at all sampling dates, root biomass in the inserted cores decreased with increased compaction of the plough layer (0–25 cm) as well as the subsoil (25–60 cm). Root biomass in the subsoil was low. In 1985 (with wheat), the effects of compaction in the subsoil were similar, although root biomass was greater than in 1984. However, in the plough layer there were significant differences in root biomass on only one sampling date. The mesh bag technique should be a useful complement to other field methods in studies of relations between physical soil characteristics or tillage treatments and root growth.  相似文献   

A novel approach to estimating the competitive ability of Cirsium arvense in cereals using unmanned aerial vehicle imagery     

J Rasmussen  J Nielsen 《Weed Research》2020,60(2):150-160

Eight experiments were carried out in Denmark to determine the yield loss of spring barley due to Cirsium arvense in farmers' fields and to suggest and evaluate a novel approach for quantifying C. arvense infestation in large plots. Literature about the competitive ability of C. arvense is old, scattered and inconclusive, and existing models for estimating crop yield loss are based on data from North America. This study showed that C. arvense coverage could be quantified from unmanned aerial vehicle imagery using a manual image analysis procedure. This gave similar results as scoring the coverage. Yield loss of spring barley due to C. arvense infestation assessed at harvest was given by Y = 100·(1−exp(−0.00170·X)) where Y is the percentage of crop yield loss and X is the percentage of C. arvense coverage. The yield loss was much lower than estimates from models that have been developed in North America. It is speculated that the main reason for this is the later emergence of C. arvense than the crop due to lower soil temperatures in spring. Grain moisture increased linearly with C. arvense coverage: M = 0.0310·X where M is the proportional (%) increase in grain moisture and X is the proportion (%) of C. arvense coverage. Automated image analysis procedures are needed to estimate C. arvense coverage on field scales, and further experiments are needed to reveal whether the low competitive ability of C. arvense found in this study is representative for Northern Europe.  相似文献   

Effects of cultural methods and physical weed control on intrarow weed numbers, manual weeding and marketable yield in direct-sown leek and bulb onion     

B Melander  & G Rasmussen 《Weed Research》2001,41(6):491-508

Manual weeding of intrarow weeds in direct-sown leek and bulb onion crops grown organically can be very labour-intensive. Four field experiments, two in direct-sown leek and two in direct-sown bulb onion were made in this investigation to study the effects of physical and cultural methods on intrarow weed numbers, time consumption for hand-weeding and marketable yield parameters. The physical methods considered were: pre-emergence flaming and harrowing, and post-emergence hoeing close to the row (only leek) and vertical brush weeding. The cultural methods were: seed priming, slurry placement and cultivar choice (only leek). Generally, flaming plus brush weeding gave the highest intrarow weed control at 92% and 87%, respectively, in the two leek experiments and 39% (only brush weeding was effective) and 74%, respectively, in the two onion experiments. Time consumption for hand-weeding after the different treatments was linearly related to the remaining numbers of intrarow weeds, with no significant influences of the experimental factors on the general relationship. Generally, the cultural methods had no significant influence on the effects of physical weeding in terms of their effect against intrarow weeds. They did not affect the tolerance or robustness of the crop plants against negative impact from the physical control methods. However, generally, seed priming and cultivar choice did improve yield in the leek experiments and seed priming also did so in the one experiment with onion.  相似文献   

Concentrations of trimethoprim and sulphadoxine in tissues from goats and a cow.     

P Nielsen  F Rasmussen 《Acta veterinaria Scandinavica》1975,16(3):405-410

The concentration of trimethoprim and sulphadoxine in plasma and tissue from goats and a cow have been determined after a single intravenous injection. Furthermore, the concentration of the two drugs and their metabolites in plasma and tissues have been determined after continuous intravenous infusion for 2½–3 hrs. Trimethoprim was present in all tissues but brain at higher concentrations than in plasma while the concentration of sulphadoxine in the different tissues were lower than in plasma. The highest concentration of the 2 drugs and their metabolites was found in the kidney. The distribution pattern of trimethoprim and sulphadoxine was similar in cow and goats.  相似文献