胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位     

欧承刚  孙婷婷  刘新艳  李成江  徐东辉  赵志伟  庄飞云 《园艺学报》2017,44(2):288-296

以白色的野生胡萝卜‘松滋野生’(Ws)和橘色的栽培胡萝卜品种‘Amsterdam’(Af)为亲本构建的回交重组自交系(BIL)为试材,基于低倍重测序技术开发SNP标记,构建了由1 976个Bin标记组成,包含29 435个SNP标记的遗传图谱。图谱总距离834.28 c M,平均图距0.42 c M。通过对胡萝卜肉质根中类胡萝卜素含量相关QTL分析,在连锁群LG04和LG08中检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、叶黄素、玉米黄质和总类胡萝卜素含量的主效QTL(M-QTL)2、2、3、2、2和2个,表型贡献率为11.47%~19.18%;另检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、玉米黄质和总类胡萝卜素含量的上位性QTL(E-QTL)1、1、2、1和1个,表型贡献率为2.50%~3.66%。在M-QTL显著区间内共检索到36个有功能注释的预测基因,其中Dck018297为ζ–胡萝卜素脱氢酶2基因,与调控β–胡萝卜素合成和总类胡萝卜素含量有关;Dck008006为乙烯响应因子2.2的同源基因,与调控α–胡萝卜素、ζ–胡萝卜素合成有关;Dck029898为转录因子b HLH135的同源基因,与调控玉米黄质合成有关。  相似文献   

不同生物刺激剂对鲜食玉米产量和品质的影响     

应武  骆乐谈  阮松林  李金文 《中国农学通报》2020,36(17):89-94

为研究生物刺激剂对玉米的增产提质效果,试验探讨不同生物刺激剂对玉米的产量和品质方面的影响,为鲜食玉米的优质高产栽培提供技术支撑。以鲜食玉米‘钱江糯3号’为试验品种,以3种生物刺激剂（阿美滋、美基、保康灵1号）为供试药剂,设置6个处理（阿美滋1000倍液、阿美滋1500倍液、阿美滋2000倍液、美基3000倍液、保康灵1号300倍液、清水对照）,研究大田常规施肥水平下药剂对‘钱江糯3号’的经济性状、产量及品质的影响。在阿美滋1500倍液喷施下,玉米的行粒数、单穗鲜重和单穗净重、产量均最高,蛋白质含量、粗纤维含量、还原糖含量、淀粉含量均居第3位;在保康灵1号300倍液喷施下,玉米的穗粗最高,单穗鲜重、单穗净重、产量均居第2位,蛋白质含量最高,脂肪含量最低,还原糖含量居第2位。阿美滋1500倍液在增加玉米产量上是最佳选择,保康灵1号300倍液则对提升玉米品质最有效果。建议生产上将阿美滋1500倍液与保康灵1号300倍液配合施用,这对鲜食玉米的增产提质效果最好。  相似文献   

Effects of dietary oregano essential oil supplementation on growth performance,intestinal antioxidative capacity,immunity, and intestinal microbiota in yellow-feathered chickens     

Dong Ruan  Qiuli Fan  Ahmed Mohamed Fouad  Yunyun Sun  Shengshu Huang  Aiji Wu  Chuxiao Lin  Zhixiang Kuang  Chang Zhang  Shouqun Jiang 《Journal of animal science》2021,99(2)

Essential oils are plant-derived aromatic volatile oils, and they contain bioactive compounds that have been shown to improve poultry nutrition. In this study, we investigated the effects of oregano essential oil (OEO) on intestinal antioxidative capacity, immunity, and gut microbiota of young yellow-feathered chickens. A total of nine hundred and sixty 1-d-old female Qingyuan partridge chickens were randomly allocated to four treatment groups with six replicates of 40 birds each, and the feeding trial was lasted for 30 d. The controls were fed on a basal diet without in-feed antibiotics; the birds in the antibiotic group were fed the basal diet supplemented with 20 mg/kg virginiamycin; the remaining birds were fed the basal diet containing 150 or 300 mg/kg OEO, respectively. Dietary supplementation with 150 or 300 mg/kg OEO increased average daily feed intake (P = 0.057) and average daily gain (P < 0.05). The activities of glutathione peroxidase and total antioxidative capacity in plasma, jejuna, and ileal mucosa were increased by OEO supplementation (P < 0.05), with a trend of lower jejunal content of malonaldehyde (P = 0.062). Moreover, dietary OEO increased the content of secretory immunoglobulin A (P = 0.078) and the relative expression of Claudin 1, Mucin 2, and Avain beta-defensin 1 in ileum (P < 0.05). Sequencing data of 16S rRNA indicated that dietary OEO increased the relative abundance of Firmicutes phylum, and Clostridium and Lactobacillus genera, and decreasing that of Romboutsia. Functional analyses indicated that microbial amino sugar and nucleotide sugar metabolism, replication, and repair systems were higher in OEO groups than those of controls and antibiotic treatment. In conclusion, dietary supplementation with OEO enhanced growth performance, alleviated local oxidative stress in intestine, improved production of natural antibodies, and favorably modulated intestinal microbiota composition.  相似文献   

Physiological response of one of South Africa’s premier freshwater sport angling species,the Orange-Vaal smallmouth yellowfish Labeobarbus aeneus,to catch-and-release angling     

Nico J Smit  Ruan Gerber  Richard Greenfield  Glyn Howatson 《African Zoology》2016,51(1):61-67

The practice of catch-and-release fishing has been widely promoted by angling fraternities as a fisheries manage- ment tool. The aim of this investigation was to determine the physiological response of Orange-Vaal smallmouth yellowfish, Labeobarbus aeneus, to catch-and-release angling in the Vaal River, South Africa. Fish were collected using standard fly-fishing techniques, anaesthetised in clove oil and blood was drawn from the caudal vein; thereafter the fish were weighed, measured, revived and released. Blood plasma was analysed for concentra- tions of glucose, cortisol and lactate to determine the effects of angling duration, fish size and water tempera- ture. Larger fish were angled for a longer duration compared with smaller fish. Levels of glucose were affected by water temperature (influenced by time of year). Plasma glucose concentrations decreased with greater angling duration. Few individuals (n = 12) showed increased plasma cortisol concentrations. In extended-capture fish (angled for >1 min), lactate concentrations increased significantly above values for rapid-capture fish (angled for >30 s). These data suggest that catch-and-release causes physiological stress to fish, but nonetheless this practice can be a valuable fisheries management tool to ensure the sustainability of fish populations. Other factors beyond the ‘angling’ time are likely to contribute to physiological disruptions in homeostasis and therefore handling and air exposure of angled fish should be included in future catch-and–release angling studies. In addition, the longer-term impact of angling on fish health should also be determined.  相似文献   

From killer to carer: steroid hormones and paternal behaviour     

Ruan de Bruin  Andre Ganswindt  Aliza le Roux 《African Zoology》2016,51(4):173-182

Mammalian parental investment (i.e. care of descendant offspring) is largely biased towards maternal contributions due to the specific feeding needs of mammalian offspring; however, varying degrees of paternal investment have been reported in about 10% of all mammalian species. Within the order Carnivora, paternal contribution to rearing offspring is particularly high: an estimated 32% of all studied carnivore species exhibit direct paternal care. Despite the prominence of paternal investment in carnivores, the endocrine basis of this behaviour is not well understood. This review examines the current – highly constrained – state of knowledge about the endocrine basis of carnivore paternal investment. We attempt to link changes in androgen and glucocorticoid levels with variation in direct and indirect paternal care behaviour making specific predictions regarding the way forward. Well-studied species, such as bat-eared foxes (Otocyon megalotis), dwarf mongoose (Helogale parvula) and meerkats (Suricata suricatta), where social dynamics are relatively well understood, can act as ideal model systems through which we may further investigate the endocrine basis of paternal investment in carnivores.  相似文献   

红酵母的性质及其应用研究   总被引：10，自引：0，他引：10  

李臣  阮榕生  林向阳  陈卫江  罗爱香  陈金海 《农产品加工．学刊》2006,(5):20-22,25

红酵母是酵母菌的一个属,腐生,分布广泛,在医学、食品、化工和农业等方面都有一定的应用,但目前并没有引起人们足够的重视。综述了红酵母的种属特性、理化特性、培养及其应用。  相似文献   

茶树钾营养特性的研究   总被引：5，自引：0，他引：5  

王晓萍  阮建云 《作物学报》1995,21(3):324-329

本文对我国主要茶区茶园土壤的含钾状况，茶树品种和土壤水分对茶树吸收钾的影响和茶园施肥钾肥的增产效果进行了系统研究。结果表明，我国有近６０％的茶园土壤处于罐钾状态，这已成为限制茶叶高产优质的主要障碍因子之一。不同品种茶树对钾的吸收具有明显差异，它们对钻的最大速率Ｖｍａｘ为龙井４３＞碧云＞苹云＞碧峰＞菊花春，米氏亲和常数Ｋｍ为碧峰＞碧云＞龙井４３＞苹云＞菊花春。干旱处理的茶树体内累积的钾和叶片的含钾量  相似文献   

BY-150型种子包衣机检测控制系统设计   总被引：1，自引：0，他引：1  

桑杰  赵春宇  朱成刚 《农机化研究》2015,(3):83-86

包衣机在批量加工流程中需要针对供种量和进液量等重要参数进行检测,否则无法保证良好的包衣效果。为此,设计了一种包衣机检测控制系统,可实现对供种量和进液量参数信息的检测和控制。同时,需在供种量检测模块的硬件选型和电路设计中采用尽可能多的抗干扰措施保证种子质量的检测精度,在进液量检测模块的设计中通过采用主板信号控制动作启动、传感器检测电路直接控制动作结束的方式保证进液控制的稳定性,并通过液晶触摸屏为用户提供一个简单直观的人机交互方式。整个系统稳定可靠、抗干扰性好,满足预期的精度要求,包衣效果良好。  相似文献   

用Delphi实现温度传感器的数据采集和误差分析   总被引：1，自引：0，他引：1  

柴成钢  陈建军 《华中农业大学学报》2002,21(3):292-294

利用Delphi嵌入式汇编的扩展功能实现基于I／O的热敏电阻温度传感器数据采集，并用二次样条插值法研究电压和温度特性非线性化的S曲线，减少非线性误差。  相似文献   

风送式喷雾机导流器结构优化及试验研究   总被引：1，自引：5，他引：1  

宋淑然  夏侯炳  卢玉华  洪添胜  阮耀灿 《农业工程学报》2012,28(6):7-12

风送式喷雾机风筒内部结构影响风场的分布及喷雾机的效率。该文利用仿真方法,研究了风筒内导流片数目对内部流场的影响;仿真研究柱形导流器、锥形导流器及半椭球形导流器结构对风筒流场的出风速度、压力损失的影响;以效率高为优化目标,优化导流器的形式及结构;利用试验样机,对安装优化后导流器的风筒进行了实际测量,并与导流器优化前的测量结果进行了对比分析。仿真及试验结果表明:导流片数目一般以4～5为宜;导流器优化后,当风扇工作转速为2926.5r/min时,可节约电能4.88%。导流片的安装既有利于将风筒内的旋转气流转化为轴向的气流,同时又产生压力损失;导流器的结构对风筒的压力损失率、出风口风速产生较大的影响,其中半椭球导流器产生的压力损失率最小。  相似文献