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An 8‐week feeding trial was conducted to establish the dietary vitamin E requirement of juvenile cobia. The basal diet was supplemented with 10, 20, 30, 40, 60, 120 mg vitamin E kg?1 as all‐rac‐α‐tocopheryl acetate. The results indicated that fish fed the diets supplemented vitamin E had significantly higher specific growth rate, protein efficiency ratio, feed efficiency and survival rate than those fed the basal diet. It was further observed that vitamin E concentrations in liver increased significantly when the dietary vitamin E level increased from 13.2 to 124 mg kg?1. Fish fed the basal diet had significantly higher thiobarbituric acid‐reactive substances concentrations in liver than those fed the diets supplemented vitamin E. Fish fed the diets supplemented with 45.7 and 61.2 mg kg?1 vitamin E had significantly higher red blood cell and haemoglobin than those fed the basal diet, while fish fed the diets supplemented with 61.2 and 124 mg kg?1 vitamin E had higher immunoglobulin concentration than those fish fed the basal diet. Lysozyme and superoxide dismutase were significantly influenced by the dietary vitamin E level. The dietary vitamin E requirement of juvenile cobia was established based on second‐order polynomial regression of weight gain and lysozyme to be 78 or 111 mg all‐rac‐α‐tocopheryl acetate kg?1 diet, respectively.  相似文献   
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Leaf area index (LAI) is a key ecophysiological parameter in forest stands because it characterises the interface between atmospheric processes and plant physiology. Several indirect methods for estimating LAI have been developed. However, these methods have limitations that can affect the estimates. This study aimed to evaluate the accuracy and applicability of a visual method for estimating LAI in clonal Eucalyptus grandis × E. urophylla plantations and to compare it with hemispherical photography, ceptometer and LAI-2000® estimates. Destructive sampling for direct determination of the actual LAI was performed in 22 plots at two geographical locations in Brazil. Actual LAI values were then used to develop a field guide with photographic images representing an LAI range of 1.0–5.0 m2 m?2 (leaf area/ground area). The visual LAI estimation guide was evaluated with 17 observers in the field. The average difference between actual LAI and visual LAI estimation was 12% and the absolute difference between the two methods was less than or equal to 0.5 m2 m?2 in 77% of plots. Pearson’s correlation coefficients were high between actual LAI and hemispherical photographs (0.8), visual estimation (0.93) and LAI-2000® (0.99) and low for the ceptometer (0.18). However, absolute values differed among methods, with the average difference between the actual and estimated LAI of [12]% for visual estimation, 28% for the LAI-2000®, 37% for the ceptometer and ?43% for hemispherical photographs. The LAI-2000® and ceptometer overestimated LAI in all plots, whereas hemispherical photographs underestimated the values in all measurements, showing that these methods need calibration to be used. No differences were observed between actual LAI and visual estimates across stand ages of 2–8 years and LAI of 1.5–5.3 m2 m?2 (P > 0.05). The results show that visual estimation of LAI in Eucalyptus stands is a practical method that is unaffected by atmospheric characteristics and can be used on an operational scale.  相似文献   
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An 11‐week feeding trial was conducted to evaluate the effect of dietary methionine on the growth, antioxidant status, innate immune response and disease resistance to Aeromonas hydrophila of juvenile yellow catfish. Six isonitrogenous and isolipidic practical diets were formulated to contain different graded methionine levels ranging from 6.1 to 16.4 g kg?1 of dry weight. The results indicated that growth performance and feed utilization were significantly influenced by the dietary methionine levels; fish fed the diet containing 6.1 g kg?1 methionine level had lower specific growth rate, percentage weight gain (PWG), feed efficiency and protein efficiency ratio than those fed the other diets (P < 0.05). Fish fed the diet containing 16.4 g kg?1 methionine level had lowest protein contents in whole body and muscle among all treatments. Triacylglycerols, cholesterol, aspartate aminotransferase, alanine aminotransferase and haemoglobin (Hb) in plasma or whole blood were significantly affected by dietary methionine levels. Fish fed the diet containing 6.1 g kg?1 methionine level had higher superoxide dismutase, glutathione peroxidase activities and malondialdehyde values than those fed other diets. Fish fed diets containing 9.7 and 11.8 g kg?1 methionine levels had higher lysozyme activity, total immune globulin, phagocytic activity and respiratory burst than those fed other diets. The lowest survival after A. hydrophila challenge was observed in fish fed a diet containing 6.1 g kg?1 methionine. Quadratic regression analysis of PWG against dietary methionine levels indicated that the optimal dietary methionine requirement for the maximum growth of juvenile yellow catfish was estimated to be 11.5 g kg?1 of the diet in the presence of 4.0 g kg?1 cystine (corresponding to 23.5 g kg?1 of dietary protein on a dry weight basis).  相似文献   
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In Kenya almost all sugarcane grown is under rainfall conditions that are planted in different locations during both long and short rainy seasons. Effect of season, location, cultivar and spacing on yield and quality of sugarcane is not clearly understood. The research was conducted at Kibos and Mumias to determine if the yields and quality of three sugarcane cultivars were affected by season, location and row spacing. Cultivars CO 945, EAK 73-335 and KEN 83-737 were manually planted during June and September in 2011. The trial was laid out as split-split plot arranged in a randomized complete block design (RCBD) replicated three times. Data collected at harvest, 17 months after planting (MAP) including cane yields, cane yield components and cane quality. The results revealed that cane length differed significantly between June and September. Stalk population and cane yields were significantly more at Mumias compared to Kibos. Cultivar EAK 73-335 showed significantly more cane yield (t/ha) than KEN 83-737 and CO 945. Both row spacing 1.2 m and 1.5 m did not affect cane yields. Brix%, Sucrose% and Purity% differed significantly between cultivars. The relationship between cane yield and stalk population was positive and significant, which indicated stalk population is a strong determinant of cane yield. The results showed that cane yield was significantly affected by location and cane quality by cultivars. Therefore Mumias is a better zone for sugarcane cultivation for both June and September seasons compared to Kibos.  相似文献   
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