Effect of intramammary injection of rboGM-CSF on milk levels of chemiluminescence activity, somatic cell count, and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis          下载免费PDF全文

Hideyuki Takahashi  Masaharu Odai  Kenji Mitani  Shigeki Inumaru  Shozo Arai  Rieko Horino    Yuichi Yokomizo 《Canadian journal of veterinary research》2004,68(3):182-187

The effect of intramammary injection of recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF, 400 microg/10 mL) on quarter milk levels of chemiluminescence (CL) activity, and somatic cell count (SCC) and shedding pattern of Staphylococcus aureus was investigated. Ten Holstein cows, naturally infected with S. aureus were used, with either early-stage or late-stage subclinical mastitis. Injection of rboGM-CSF caused a remarkable increase in milk CL activity with a peak at 6 h after the cytokine injection in the early- and late-stage groups. In the early-stage group, milk SCC stayed around preinjection level at 6 h, rose significantly on days 1 and 2, and was followed by a smooth and significant decline to an under preinjection level (below 200 000 cells/mL) on day 7 postinjection. Alternatively, in the late-stage group, milk SCC rose significantly at 6 h after the cytokine injection and maintained high levels thereafter. The milk S. aureus count decreased drastically by the cytokine injection in the early-stage group. The bacterial count was moderately decreased in the late-stage group, but increased back to preinoculation levels on day 7 after the cytokine injection. The results suggest that the rboGM-CSF has a potential as a therapeutic agent for S. aureus infection causing subclinical mastitis of dairy cows, if the cytokine is applied at the initial stage of infection.  相似文献   

A morphological analysis of the skull size and shape of Kerivoulinae (Chiroptera: Vespertilionidae) from Vietnam     

Son Truong NGUYEN  Masaharu MOTOKAWA  Tatsuo OSHIDA  Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):187-198

Southeast Asia is a region of high biodiversity, containing species of plants and animals that are yet to be discovered. In this region, bats of the subfamily Kerivoulinae are widespread and diverse with six species recorded in Vietnam. However, the taxonomy of the Kerivoulinae in Asia is complicated. In our study, we used diagnostic characters and multivariate analysis to determine morphological differences between the genera Kerivoula and Phoniscus. We showed that the two genera are distinguishable by the size of second upper incisors, the shape of skull, nasal sinus, canines, second upper and lower premolars. In addition, the two genera can be osteometrically separated by measurements of the braincase height, interorbital width and shape of anterior palatal emargination. Our data clearly revealed the morphological variations in the skull shape of Kerivoula hardwickii in Vietnam. This suggests a possible separation into three morphotypes, representing cryptic species supported by statistical differences with wide variation in skull shape, size and teeth. These results demonstrated Kerivoula hardwickii can be separated three subspecies, and the result will serve as the basis for the future assessment and classification of this group in Southeast Asia.  相似文献   

A dog with myelodysplastic syndrome: chronic myelomonocytic leukemia     

Hiraoka H  Hisasue M  Nagashima N  Miyama T  Tanimoto T  Watanabe M  Itamoto K  Mizuno T  Inokuma H  Okuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):665-668

An eleven-year-old female pug was referred to Yamaguchi University Animal Hospital for evaluation of anemia and thrombocytopenia. The cytological examination of the peripheral blood showed some giant monocytic lineage blast cells. A few granulocytes and platelets had dysplastic features. On day 7, in addition to increasing the monocytic lineage cells, the dysplastic features of the blood had also increased compared to the initial examination. We performed bone marrow aspiration upon her death. The bone marrow revealed dysplastic features in all three hematopoietic cell lines, and an increase in the monocytic cell line. Based on the features of the bone marrow and the peripheral blood, this case was confirmed to be myelodysplastic syndrome--Chronic myelomonocytic leukaemia (MDS-CMML).  相似文献   

Anaphylaxis after vaccination for cats in Japan     

Megumi YOSHIDA  Keijiro MIZUKAMI  Masaharu HISASUE  Ichiro IMANISHI  Keigo KURATA  Masaki OCHIAI  Masato ITOH  Tadahiro NASUKAWA  Jumpei UCHIYAMA  Hajime TSUJIMOTO  Masahiro SAKAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):149

Severe adverse reactions in cats after vaccination were examined from 316 cases reported to the Ministry of Agriculture, Forestry and Fisheries (MAFF) in Japan during 15-year period from April 2004 to March 2019. We found that 130 (41%) showed anaphylaxis, and 99 (76%) of the 130 cases of anaphylaxis resulted in death. Veterinarians should be well prepared to deal with vaccine-associated anaphylaxis in cats. Bovine serum albumin (BSA) as indicator of purification was detected at high levels in commercially available feline vaccines. BSA might derive from fetal calf serum in culture media. This study provides useful information about anaphylaxis including critical details of the potential clinical signs associated with adverse events to feline vaccination.  相似文献   

Post-molt processes of cuticle formation and calcification in the Japanese mitten crab <Emphasis Type="Italic">Eriocheir japonicus</Emphasis>     

Konomi Sakamoto  Wataru Honto  Masaharu Iguchi  Nobuhiro Ogawa  Kazuhiro Ura  Yasuaki Takagi 《Fisheries Science》2009,75(1):91-98

This study examines the following in the Japanese mitten crab: (1) the structure of the exoskeleton with special reference to its calcification; (2) the progression of post-molt cuticle formation and calcification. In the crab, the structure and calcification state of the exoskeleton at the molt and during the inter-molt stage were similar to those of other crustaceans. During the inter-molt, the exoskeleton consisted of four cuticle layers; the outermost epicuticle, the exocuticle, the endocuticle and the innermost membrane layer. Intense calcification was observed in the exo- and endocuticle. At the molt, the synthesis of the epi- and exocuticle was already complete, and the addition of the endocuticle began after the molt. Calcification of the exocuticle initiated soon after the molt, but there was a delay between endocuticle matrix synthesis and calcification. Histology showed that the process of calcification was similar to that in other crustaceans. However, calcium concentrations within the exoskeleton continued to increase and never reached the levels of the inter-molt stage at the end of the experiment. This suggests that the Japanese mitten crab is relatively slow to calcify compared to other crustaceans.  相似文献   

Studies on the green liver in cultured red sea bream fed low level and non-fish meal diets: Relationship between hepatic taurine and biliverdin levels   总被引：1，自引：1，他引：1  

Takanobu Goto  Shusaku Takagi  Toshiaki Ichiki  Tadashi Sakai  Makoto Endo  Terutoyo Yoshida  Masaharu Ukawa  Hisashi Murata 《Fisheries Science》2001,67(1):58-63

SUMMARY: The aim of this study was to investigate the cause of green liver in red sea bream fed substitute protein diets. Red sea bream Pagrus major was given either of the following diets for 28 weeks: (1) control diet (50% fish meal), (2) low level fish meal diet (15% fish meal), and (3) non-fish meal diet (0% fish meal). The green liver was observed in all groups tested, but the incidence was much higher in the experimental diet groups. The feeding of substitute protein diets reduced plasma triglyceride and cholesterol levels. However, there was no significant difference in plasma hepatic enzyme activities and plasma bile salts concentration among the treatments. Fish fed the substitute protein diets showed low hepatic taurine levels with an appearance of a biliverdin in the liver. Moreover, the proportion of ditaurobilirubin to total biliary bile pigments was significantly lower in fish fed the substitute protein diets. These data indicate that feeding of substitute protein diets did not induce any cholestatic hepatobiliary obstructions and that the low hepatic taurine level was one of the probable factors responsible for the occurrence of green liver in red sea bream fed substitute protein diets.  相似文献   

Ceramide aminoethylphosphonate from jumbo flying squid Dosidicus gigas attenuates the toxicity of cyanotoxin microcystin-LR     

Masaharu Komatsu  Naoki Ichiyama  Takashi Kurimoto  Shota Takumi  Kazuhiro Shiozaki  Yasumasa Sugiyama  Tatsuhiko Furukawa  Seiichi Ando  Saki Itonori  Hiroaki Saito 《Fisheries Science》2013,79(2):313-320

We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR.  相似文献   

Establishing an acid deposition monitoring network in East Asia     

Masaharu Yagishita 《Water, air, and soil pollution》1995,85(1):273-278

Acid deposition is widely recognized as one of the most serious international atmospheric pollution problems. East Asian countries are currently experiencing acid deposition and have only recently begun expanding domestic research and monitoring activities. However, acid deposition is not only a domestic but also a transboundary problem. In addressing the transboundary aspect, cooperative and collaborative action will be essential. The Environment Agency of Japan is advocating the need to establish an acid deposition monitoring network in East Asia. Therefore, it is sponsoring three Expert Meetings on Acid Precipitation Monitoring Network in East Asia between 1993 and 1995. The meetings are attended by both scientific researchers and government officials from numerous East Asian countries. At the administrative and scientific discussions of the first two meetings a consensus on the desirability of establishing a monitoring network in East Asia was obtained and a preliminary technical guideline manual for monitoring acid deposition was adopted. By the end of the third meeting it is hoped that a basic consensus can be reached on a framework for an East Asian acid deposition monitoring network. It is essential to establish such a network in order to evaluate the present state of acid deposition in the region and to reach a common scientific understanding on the acid deposition problem. This is a vital step toward promoting international cooperation on the issue.  相似文献   

Clinical response of ovarian cysts in dairy cows after PRID treatment     

Zulu VC  Nakao T  Yamada K  Moriyoshi M  Nakada K  Sawamukai Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):57-62

We investigated the therapeutic effects of a progesterone releasing intravaginal device (PRID) on cystic ovarian disease (COD) and reproduction performance of cows. The possible influence of PRID on metabolic and/or health status was also examined. A total of 40 Holstein-Friesian cattle, with ovarian cystic structures, > or =2.5 cm in diameter, persisting for more than 7-14 days, without a corpus luteum (CL) were used for the study. PRID or placebos were inserted into the vagina for 12 days. Five animals lost the intravaginal device before removal and one was culled. Based on plasma progesterone concentration on the day of treatment, 20 (17 PRID and 3 placebos) of the remaining 34 cows had follicular cysts (progesterone < or =1 ng/ml) and 14 (10 PRID and 4 placebos) had luteal cysts (progesterone >1 ng/m l). Fourteen (82%) of the PRID-treated follicular cystic cows responded with formation of a CL within 14 days after treatment, and an overall conception rate of 53.8%. Likewise, 70% of the treated luteal cystic cows responded with CL formation and 71.4% conception rate. No significant differences were observed in hematocrit (Ht), white blood cell count and serum levels of glucose, blood urea nitrogen, aspartate aminotransferase, and alanine aminotransferase, between the day of PRID insertion and removal, in animals with follicular and luteal cysts. PRID treatment resulted in ovulation 2-4 days later and formation of a CL in cows that recovered.  相似文献   

Appearance of race Pfs:5 of spinach downy mildew fungus, Peronospora farinosa f. sp. spinaciae, in Japan     

Mamoru Satou  Kazufumi Nishi  Masaharu Kubota  Michiko Fukami  Hideaki Tsuji  Kees van Ettekoven 《Journal of General Plant Pathology》2006,72(3):193-194

The races for the causal agent of spinach downy mildew Peronospora farinosa f. sp. spinaciae were identified by inoculation of race-differential cultivars. One isolate was identified as Pfs:5s and the others belonged to a new race. This is the first report of race Pfs:5 and another new race in Japan.  相似文献