Effect of dietary vitamins C and E fortification on lipid metabolism in red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli     

HONG  JI  Ahmad Daud  OM  Takao  YOSHIMATSU  Masahiro  HAYASHI  Tetsuya  UMINO  Heisuke  NAKAGAWA  Masaya  ASANO  Atsushi  NAKAGAWA 《Fisheries Science》2003,69(5):1001-1009

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Application of acoustic Doppler current profiler combined with a scientific echo sounder for krill Euphausia pacifica density estimation     

Kyounghoon  LEE  Tohru  MUKAI  Donhyug  KANG  Kohji  IIDA 《Fisheries Science》2004,70(6):1051-1060

ABSTRACT:   An application of the acoustic Doppler current profiler (ADCP, 153.6 kHz) in combination with a scientific echo sounder (EK60, 38 and 200 kHz) was investigated to estimate the density of krill Euphausia pacifica . The acoustic backscattering strength from sound scattering layers was compared with biomass estimates from midwater trawls. Euphausia pacifica was targeted among mixed species populations in the sound scattering layer in the offshore Funka Bay area of Hokkaido, Japan. The frequency characteristics of acoustic backscattering by krill were calculated using a distorted wave Born approximation scattering model at three frequencies. Krill aggregations identified from the EK60 data were extracted as the mean volume backscattering strength difference between two frequencies. They were then used to identify similar aggregations in the ADCP data by matching observation times and depths for the two methods, which were applied simultaneously. Results from the comparison of the mean volume backscattering strength and the density calculated from the ADCP and EK60 showed that ADCP can be used to measure density and spatial–temporal distribution of krill aggregations. Current speed and direction at the study site were found to be 16.1 cm/s and 187.0°, respectively, and krill speed and direction (including the current component) were found to be 19.8 cm/s and 172.2°, respectively. Based on the ADCP data, the net speed and direction of the krill aggregations were found to be 5.9 cm/s and 128.0°, respectively.  相似文献   

Salinity stress responses of a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta)     

Makoto  KAKINUMA  Yoshinao  KUNO  Hideomi  AMANO 《Fisheries Science》2004,70(6):1177-1179

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Dielectric properties of surimi at 915 MHz and 2450 MHz as affected by temperature, salt and starch     

Weijie  MAO  Manabu  WATANABE  Noboru  SAKAI 《Fisheries Science》2003,69(5):1042-1047

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Effects of added hemolysate from mackerel, herring and rainbow trout on lipid oxidation of washed cod muscle     

Mark P.  RICHARDS  Herbert O.  HULTIN 《Fisheries Science》2003,69(6):1298-1300

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Na/K-ATPase activity during larval development in the giant freshwater prawn Macrobrachium rosenbergii and the effects of salinity on survival rates     

Do Thi  Thanh HUONG  Vidya  JAYASANKAR  Safiah  JASMANI  Hisako  SAIDO-SAKANAKA  Andrew J.  WIGGINTON  Marcy N.  WILDER 《Fisheries Science》2004,70(3):518-520

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Comparative pharmacokinetics and bioavailability of oxytetracycline in giant tiger prawn     

Koolvara  SANGRUNGRUANG  Amornthep  CHOTCHUANG  Ryuji  UENO 《Fisheries Science》2004,70(3):467-472

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Expression of a recombinant crustacean hyperglycemic hormone of the kuruma prawn Penaeus japonicus in methylotrophic yeast Pichia pastoris     

TSUYOSHI  OHIRA  HIDEKAZU  KATAYAMA  KATSUMI  AIDA  HIROMICHI  NAGASAWA 《Fisheries Science》2003,69(1):95-100

ABSTRACT:   Until now, six crustacean hyperglycemic hormones (CHH) designated Pej-SGP-I, -II, -III, -V, -VI and -VII have been characterized in the kuruma prawn Penaeus japonicus . All CHH consist of 72 amino acid residues and have an amidated carboxyl (C)-terminus. In the present study, we expressed Pej-SGP-III in methylotrophic yeast Pichia pastoris in order to obtain a large quantity of recombinant CHH possessing biological activity. A cDNA encoding Pej-SGP-III that had been previously cloned was processed by polymerase chain reaction (PCR) and the resulting product was ligated into an expression vector. Pichia pastoris was transformed with this vector after which a recombinant Pej-SGP-III was expressed having an additional amino acid residue (glycine) at the C-terminus (rPej-SGP-III-Gly), a form considered to be a putative precursor of this hormone. rPej-SGP-III-Gly secreted into the culture medium was purified by reversed-phase HPLC, and amidated using a peptidylglycine alpha-amidating enzyme. The amidated rPej-SGP-III (rPej-SGP-III) showed hyperglycemic activity in in vivo bioassay almost comparable to that of the natural Pej-SGP-III. rPej-SGP-III thus obtained will be a useful tool not only for its physiological study but also for the determination of its 3-D structure.  相似文献   

Pueruli of Panulirus longipes bispinosus (Crustacea, Decapoda, Palinuridae) stranded on the beach of Kuroshima Island, Ryukyu Archipelago, southern Japan     

NARIAKI  INOUE  HIDEO  SEKIGUCHI  HIROSHI  MISAKI 《Fisheries Science》2002,68(2):332-340

ABSTRACT: Stranded organisms were observed on the sandy beach of Kuroshima Island, Ryukyu Archipelago, in the early morning of 28 January 1993. Stranded organisms of 525 individuals were classified into 74 species. These organisms included 13 individuals of Panulirus pueruli, which were identical to P. longipes bispinosus. Kuroshima Island is located near Ishigakijima and Iriomote islands, Ryukyu Archipelago, where coral reefs develop. Coral reefs are common and develop in the Ryukyu Archipelago, southern Japan, with the exception of Yakushima and Tanegashima islands. Panulirus japonicus in Japanese waters has not been found in coral reef regions where P. longipes bispinosus are mainly found. The present study describes and identifies to species level puerulus specimens that were stranded on the beach of Kuroshima Island.  相似文献   

Powdery Mildew of Prairie Gentian: Characteristics,Molecular Phylogeny and Pathogenicity   总被引：3，自引：0，他引：3  

OKAMOTO  Jun  LIMKAISANG  Saranya  NOJIMA  Hidenobu  TAKAMATSU  Susumu 《Journal of General Plant Pathology》2002,68(3):200-207

In March 1999, we found prairie gentian (Eustoma grandiflorum) infected with powdery mildew in a greenhouse in Oita Prefecture, Japan. Morphological observation revealed that the causal fungus belongs to the mitosporic genus Oidium subgenus Pseudoidium [teleomorph: Erysiphe sensu Braun and Takamatsu (2000)]. Precise taxonomic position of the fungus, however, is uncertain due to lack of the perfect stage. We determined the nucleotide sequence of the rDNA ITS region of the fungus. Comparison of the sequence with those obtained from DNA databases of this fungal group revealed that the sequence is identical to those of powdery mildews from garden four-o'clock (Mirabilis jalapa) and broad bean (Vicia faba). Inoculation of an isolate from garden four-o'clock caused mildew on prairie gentian and broad bean, suggesting that the prairie gentian mildew originates from garden four-o'clock or broad bean. Molecular phylogenetic analysis indicated a close relationship of this fungus to Erysiphe baeumleri on Vicia spp. and E. trifolii on Trifolium pratense. From these results, we propose that prairie gentian mildew diverged from a Fabaceae-parasitic ancestor. Received 14 March 2002/ Accepted in revised form 28 May 2002  相似文献