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Mutsumi NAKAMURA Takafumi NAGAMINE Akio TAKENAKA Rustem I. AMINOV Koretsugu OGATA Kiyoshi TAJIMA Hiroki MATSUI Yoshimi BENNO Hisao ITABASHI 《Animal Science Journal》2002,73(5):347-352
A 2.6-kb DNA fragment encoding a xylanase gene ( xyn A) was cloned from the rumen hemicellulolytic bacterium Ruminococcus albus 7. The deduced primary structure of the protein (XynA) was divided into a signal peptide region and 3 domains. Domain A was identified as a family 11 (G) catalytic domain, but one amino acid residue was replaced by another in an active site signature 1 of family 11. Domain B is a stabilizing domain for the catalytic domains of families 10 and 11. Deletion of domain B reduced stability of the xylanase at high temperature and at high and low pH. Domain B may be useful for protein engineering of xylanase. Domain C has sequence similarity to deacetylases and NodB proteins. 相似文献
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Maria R. M. Coimbra Kazunobu Kobayashi Shinrokuro Koretsugu Osamu Hasegawa Eriko Ohara Akiyuki Ozaki Takashi Sakamoto Kyoshi Naruse Nobuaki Okamoto 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):203-218
We report the first genetic linkage map of the Japanese flounder (Paralichthys olivaceus) constructed with 111 microsatellite markers and 352 AFLP fragments. The parental male linkage map consisted of 25 linkage groups while the female map consisted of 27 groups, with an average resolution of 8 and 6.6 cM, respectively. We have identified linkage among 96% of the markers and the total map length was estimated to be around 1000–1200 cM. This study reports the first low-density linkage map for the Japanese flounder and describes differences in sex recombination. Recombination rates were higher in male flounder compared to the female (7.4:1), a rare condition among vertebrates. This map is a starting point for the mapping of single loci and quantitative traits in flatfish species. 相似文献
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Sayaka TSUCHIDA Takanari HATTORI Akiko SAWADA Koretsugu OGATA Jun WATANABE Kazunari USHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):1012
We performed a comprehensive fecal metabolite analysis using LC-MS/MS and LC-QTOF-MS approaches as a preliminary study. Feces of Japanese macaques on Yakushima Island were collected from five monkeys at two separate locations. Using the former methodology, 59 substances such as free amino acids, nucleotides, nucleosides and nucleic acid bases, and organic acids in the citrate cycle were quantitatively detected and successfully differentiated in two different monkey groups by the concentrations of nucleic acid metabolites and free amino acids. In the latter, around 12,000 substances were detected both by positive and negative mode in each sample. Differences in signal intensities were observed between two monkey groups in the concentrations of plant secondary metabolites such as cyanogenic glycosides, flavonoids, and phenolics. 相似文献
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