应用FA及PPA-ELISA技术对猪瘟的检测   总被引：3，自引：0，他引：3  

潘树德  何利昆  李学俭  马兴元 《动物医学进展》2003,24(5):114-116

用免疫荧光抗体诊断技术及单克隆抗体纯化酶联免疫吸附试验诊断技术对疑为猪瘟病毒感染猪进行了检测 ,重点阐述了 2种方法的技术原理和操作过程。通过用免疫荧光抗体诊断技术检测了 5份病料 ,其中有 2份为阳性 ,阳性率为 40 % ;用单克隆抗体纯化酶联免疫吸附试验诊断技术检测了猪瘟血清抗体 ,在 40头母猪血清样品中 ,猪瘟弱毒抗体效价 OD值较高 ,有 1 0 0 %的保护率 ,但是发现母猪群中有 1 0 %隐性猪瘟感染 ,其强毒抗体效价 OD值大于 0 .5,体内带有猪瘟病毒。结果及过程表明此两种诊断方法检测快速、鉴别准确、分辨率高  相似文献   

High efficient expression of human endostatin in E.coli and its antiangiogensis activity     

ZHANG Ge  KE Xi-song  DING Ai-ling  YING Wei  YANG kun  ZHU Zhen-yu 《园艺学报》2002,18(11):1373-1375

AIM: To examine the expression of human endostatin in E.coli, produce its fusion protein antibody and observe its biological activity. METHODS: Endostatin gene was amplified by polymerase chain reaction,recombined with plasmid vector pGEX-2T and induced expression with IPTG.The protein activity was tested by endothelial cell proliferation inhibitory assay.Inclusion body crudely purified was used to generate polyclonal antibody to detect its expression at mouse's liver and kidney etc. RESULTS: The protein expressed was 20kD after digestion by thrombin,it appeared the anti-angiogenesis activity and Western blotting indicated the expression of endostatin in liver and kidney of mouse. CONCLUSION: The successful expression of human endostatin and the preparation of polycolonal antibody indicated its potential application in anti-angiogenesis therapy and diagnosis tumors.  相似文献   

枯草芽孢杆菌B—916防治水稻纹枯病的田间试验   总被引：35，自引：4，他引：35  

陈志谊  高太东 《中国生物防治》1997,13(2):75-78

１９９２～１９９４年在田间小区试验比较两个拮抗细菌（Ｂ－９１６、Ｐ－９１）和井岗霉素对水稻纹枯病的防治效果。１９９４～１９９５年在江苏省３个基点上进行了Ｂ－９１６防治纹枯病的大面积示范试验。Ｂ－９１６发酵液在田间对水稻纹枯病的为害有较强的控制能力，发酵含菌量为２．５４×１０１１～２．５４×１０１２ｃｆｕ／ｍＬ时，用量３．７５Ｌ／ｈｍ２的防治效果为５０．０％～８１．０％。  相似文献   

复杂疾病的遗传咨询技术     

张堃  裴雨晨  杨进  董靖 《广西农业生物科学》2014,(4):916-919

复杂疾病大面积爆发使人们的生活健康面临着重大挑战。在这一环境下,遗传咨询在人们的生活中占据了越来越重要的地位。依据基因与环境互作共同影响个体健康的原理,咨询者在遗传咨询师的指导帮助下合理解决各种遗传学问题可以有效降低复杂疾病的发病率。目前国内的遗传咨询发展尚处于萌芽期,随着遗传咨询在健康领域的地位上升,该技术在未来将会进一步推广应用。  相似文献   

CAN总线在大型鸡舍温度测控系统中的应用     

韩玉坤 《农机化研究》2004,(4):168-169

大型鸡舍的环境温度控制对于肉鸡的饲养有着重要的意义。为此，介绍了基于CAN总线的鸡舍温度测控系统的设备组成和工作原理，重点叙述了智能节点的软硬件设计。  相似文献   

鸡骨型白血病免疫琼扩与放射摄片诊断的比较   总被引：1，自引：1，他引：0  

陈白希  白朝勇  单玉萍  岳学民  詹国英  吴玄光  詹耀明  钟奋军  林景坤  黎建荣 《中国兽医学报》2003,23(5):490-492

为了探索特异性免疫学诊断方法对鸡骨型白血病(OP)的检测效果。用双盲法分批采样同时进行羽髓琼脂扩散试验和胫骨X线摄片检查对比,共检834只鸡(433羽属天然自发OP鸡群,401羽属人工诱发实验鸡群)。结果羽髓琼扩试验的OP阳性检出率为3．48％(29／834：)。阳性病理符合率为75．86％(22／29)。阳性误诊(假阳性)率24．13％(7／29),阴性误诊(假阴性)率36．15％(291／805)。而X线摄片检查的OP阳性检出率为38．37％(320／834)。X线阳性完全可获病理组织学证实,不存在假阳性或假阴性。把禽白血病羽髓琼扩试验试用于检测鸡骨型白血病尚属初次报道,其阳性检出率低而误诊率高,尚不宜作OP检测手段。放射摄片诊断的阳性检出率高而误诊率低,可快速诊断,故适用于OP检疫。  相似文献   

In situ rumen degradation and in vitro gas production of some selected grains from Turkey     

Umucalilar HD  Coşkun B  Gülşen N 《Journal of animal physiology and animal nutrition》2002,86(9-10):288-297

An investigation of the dry matter degradability (DMD) and effective dry matter degradability (EDDM) was performed for barley, wheat, rye, corn, triticale and oat samples, using the Nylon-bag technique. Gas production (GP), metabolizable energy (ME) and in vitro organic matter digestibility (IVOMD) were also studied by using Hohenheim gas test. The DM from barley, wheat, rye and triticale was digested rapidly in the rumen, and, at the 48 h of incubation, degradability was found to be approximately about 80%. The higher degradability observed for these grains than for oats and corn was attributable to the structure of these grains. In contrast, DM of corn and oats was degraded very slowly and reached 66.7 and 66.5 at 48 h, respectively. Effective degradability values of barley, wheat, rye, corn, triticale and oats were determined to be 61.4, 69.0, 64.0, 41.7, 66.7 and 58.6% in 5% rumen outflow rate, respectively. At the end of the 48 h incubation, total gas productions in barley, wheat, rye, corn, triticale and oats were estimated to be 83.6, 87.2, 87.5, 83.5, 85.8 and 63.9 ml/200 mg DM, respectively. The mean ME values of these grains calculated from cumulative gas amount at 24 h incubation were 11.8, 12.1, 12.3, 10.9, 12.4 and 10.2 MJ/kg DM, respectively. In vitro digestible organic matter of barley, wheat, rye, corn, triticale and oats were estimated to be 85.0, 87.3, 88.2, 79.5, 89.0 and 72.6%. Percentage overall EDDM (k=5%) of barley, wheat, rye, triticale and oats was positively correlated with in vitro GP at 6 h, cumulative GP at 24 h and total GP at 48 h (p<0.05). As a result, in situ dry matter degradation of grains showed great differences depending on the chemical compositions. In situ EDDM of grains may be predicted from in vitro gas production parameters.  相似文献   

气相色谱法测定青椒中9种农药残留方法的研究     

马丽娜  邵彦来  李鹏坤  何贶  游永  王青霞  郝小磊  曹颖 《农药科学与管理》2007,28(10):11-14

本文介绍了9种常用农药在青椒中的残留检测方法,选用VarianCP-3800气相色谱仪,cpsil-830m×0.25mm×0.25μm毛细管柱,电子捕获检测器(ECD)。平均回收率为73.2%～105.4%,变异系数<10%。该方法的测定结果满足农药多残留检测要求。  相似文献   

苹果IRAP技术体系的建立及优化   总被引：1，自引：0，他引：1  

贾怀志  刘艳红  渠慎春  高志红  王昆  章镇 《果树学报》2009,26(2)

通过苹果逆转座子长末端重复序列(LTR)保守区域设计引物,对影响苹果IRAP反应的重要因素进行了研究,建立并优化了苹果IRAP分子标记技术体系。优化的苹果IRAP分子标记体系为:25μL反应体系中,模板DNA50ng、Mg2+2.5mmol·L-1、dNTPs0.2mmol·L-1、10×buffer2.5mmol·L-1、TaqDNA聚合酶1.0U、引物0.4μmol·L-1。该体系在所试苹果品种中均获得了较理想的扩增效果,并能鉴定部分芽变品种。  相似文献   

诏安县九候山森林公园森林旅游资源现状及开发利用   总被引：2，自引：0，他引：2  

沈坤发 《林业勘察设计》2001,(2):85-87

全面叙述诏安县九候山森林公园的森林旅游资源特点,对九候山森林公园的森林旅游资源现状进行分析,提出如何开发九候山的森林旅游资源和发展九候山森林公园的旅游业。  相似文献