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Ziyad Al-Kass Alexander Brown Anders Johannisson Theodoros Ntallaris Jane M. Morrell 《Reproduction in domestic animals》2021,56(6):848-856
Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective of this study was to determine the variation among stallions in the effects of SLC on sperm quality during cooled storage for up to 48 hr. Semen samples from seven stallions (18 ejaculates) were split, with one portion being used for SLC and the other serving as a control (CON). Sperm quality (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) and chromatin integrity) were analysed at 0, 24 and 48 hr using computer-assisted sperm analysis and flow cytometry. Sperm quality was better in SLC than in CON at all timepoints, especially chromatin integrity and MI (p < .0001 for both), and some categories of ROS production (e.g. proportion of live hydrogen peroxide negative spermatozoa, p < .0001), but the degree of improvement varied among stallions and type of ROS (p < .05–p < .0001). Total and progressive motility were also better in SLC samples than in CON at 24 and 48 hr (p < .0001), although the effect on sperm kinematics varied. The interaction of treatment, time and stallion was not significant. In conclusion, sperm quality was better in SLC samples than in CON, although there was considerable individual variation among stallions. The improvement in sperm quality, particularly in chromatin integrity, was clearly beneficial, and therefore the use of this technique would be warranted for all stallion semen samples. 相似文献
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Persson Waller K Grönlund U Johannisson A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(3):121-127
Udder health problems associated with Staphylococcus aureus infections in dairy cows are difficult to control and antibiotics have limited effects. Lately, more interest has been directed towards ways to stimulate the innate immune mechanisms of the animal for better prevention and treatment of mastitis. The objectives of this study were to investigate if intramammary infusion at drying off with the immune modulator beta1,3-glucan can make the udder more resistant to experimental intra mammary S. aureus infection at this time, and to study if intramammary infusion of beta1,3-glucan into lactating udder quarters with chronic subclinical S. aureus infection can stimulate the clearing of the infection. Another aim was to evaluate the effect of beta1,3-glucan on the expression of major histocompatibility complex (MHC class II) on mammary leucocytes, measured by flow cytometry, during these circumstances. The results indicated a slight, but not statistically significant, positive effect of beta1,3-glucan at drying off on the clinical and anti-bacterial response to S. aureus infection, but no therapeutic effect of beta1,3-glucan treatment of udder quarters with chronic subclinical S. aureus mastitis. However, the proportion of MHCII+ milk lymphocytes tended to increase after glucan infusion in those udder quarters indicating a stimulation of the antigen presenting ability. To further evaluate a possible preventive effect of beta1,3-glucan infusion at drying off more studies are needed involving a larger number of animals. 相似文献
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Anders Berglund Olof Broman Johan Oja Anders Grönlund 《Scandinavian Journal of Forest Research》2015,30(1):87-97
To define new grading rules, or to customize the ones in use in a rule-based automatic grading (RBAG) system of boards, is a time-consuming job for a sawmill engineer. This has the effect that changes are rarely made. The objective of this study was to continue the development of a method that replaces the calibration of grading rule settings by a holistic-subjective automatic grading, using multivariate models. The objective was also to investigate if this approach can improve sawmill profitability and at the same time have a satisfied customer. For the study, 323 Scots pine (Pinus sylvestris L.) boards were manually graded according to the preferences of an important customer. That is, a customer that regularly purchases significant volumes of sawn timber. This manual grading was seen as reference grading in this work. The same boards were also scanned and graded by a RBAG system, calibrated for the same customer. Multivariate models for prediction of board grade based on aggregated knot variables, obtained from the scanning, were calibrated using partial least squares regression. The results show that prediction of board grades by the multivariate models were more correct, with respect to the manual grading, than the grading by the RBAG system. The prediction of board grades based on multivariate models resulted in 76–87% of the boards graded correctly, according to the manual grading, while the corresponding number was 63% for the RBAG system. 相似文献
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The fibrillar orientation in the S2-layer of wood fibres as determined by X-ray diffraction analysis
Summary It is the fibrillar orientation in the S2-layer which to a great extent determines the mechanical properties of the wood fibre, with regard both to strength and stiffness and to swelling properties. Measurements of the average fibril angle of fibres are not however easy and the results differ between the methods used. In order to evaluate in more detail how the fibril angle varies in spruce wood, an X-ray method based on diffraction from the 040-plane was developed. By comparison with microscopic examination it is concluded that reliable results relating to the fibrillar orientation in the S2-layer are obtained with the X-ray technique. It is shown that the fibril angle of mature wood is rather constant with regard to both age of the annual ring and its position in the height of the tree. The fibril angle of the earlywood is found to be only slightly higher than that of latewood fibres. It is also shown that compression wood may be easily identified by virtue of the fact that its fibril angle is much higher than that of normal mature wood.The authors thank Ms Ulla Jonsson for the microscopic measurements and Dr Anthony Bristow for the linguistic revision 相似文献
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关注林权:中国集体林政策研究国际研讨会综述 总被引:1,自引:0,他引:1
公共资源面对的挑战、风险与机遇受到国际社会的关注。文章以“国际公共资源研究协会第十届大会”为背景,对中国集体林研究课题的成果、相关国际会议内容及专家发言作了介绍。研究成果主要形成8个专题报告,国际会议分“中国集体林政策和法律框架的总体回顾”和“中国集体林与生态保护、公司 社区模式”两个部分展开,并形成了5点结论。 相似文献
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Olaf Anders 《Journal of pest science》1958,31(7):101-107
Ohne ZusammenfassungDie vorliegenden Untersuchungen wurden auf Anregung von Herrn Prof. Dr.-Ing. E. Schimitschek und mit Unterstützung der Landesforstverwaltungen Hessen und Schleswig-Holstein durchgeführt. 相似文献
10.
Göransson A 《Tree physiology》1999,19(2):111-116
Small birch plants (Betula pendula Roth) were cultivated in a hydroponic spray solution where the relative growth rate (R(G); day(-1)) was controlled by the relative supply rate of zinc (R(Zn); day(-1)). After an adjustment phase to steady-state growth, R(G) equaled R(Zn). The R(Zn) treatments were 0.05, 0.125 and 0.20 day(-1) with free access to all other nutrients. In an additional treatment, there was free access to all nutrients, including zinc (FA treatment). The pH of the nutrient solution was approximately 4.5 and conductivity was 100 &mgr;S cm(-1). The duration of each treatment depended on R(Zn) and ranged from 4 (FA treatment) to 10 weeks (at R(Zn) = 0.05 day(-1)). The plants showed persistent and typical zinc-deficiency symptoms at steady-state growth when R(G) was 0.05 and 0.125 day(-1), whereas there were few symptoms when R(G) was 0.2 day(-1). The Zn concentration of the plants ranged from 8 (at R(Zn) = 0.05 day(-1)) to 21 &mgr;g g(DM) (-1) (DM = dry mass) (at R(Zn) = 0.2 day(-1)) and was approximately 42 &mgr;g g(DM) (-1) in the FA treatment. Uptake rates of Zn, calculated per root growth rate (&mgr;mol g(DM, root) (-1)), were about 2.8 times higher at R(Zn) = 0.20 day(-1) than at R(Zn)= 0.05 day(-1). The root and stem biomass fractions were approximately constant at all supply rates of Zn, whereas the leaf biomass fraction tended to increase with increasing supply rate of Zn. Net assimilation rate was constant from FA to an R(Zn) of 0.125 day(-1) but decreased by a factor of about 2 at R(Zn) = 0.05 day(-1). Leaf area ratio and specific leaf area were smaller at low supply rates of zinc than at high supply rates. 相似文献