In vitro bactericidal activity of 465–470 nm blue light phototherapy and aminolevulinic acid on Staphylococcus pseudintermedius          下载免费PDF全文

Seulgi Bae  Taeho Oh 《Veterinary dermatology》2018,29(4):296-e102

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Whole-genome sequencing identifies missense mutation in GRM6 as the likely cause of congenital stationary night blindness in a Tennessee Walking Horse     

Yael L. Hack  Elizabeth E. Crabtree  Felipe Avila  Roger B. Sutton  Robert Grahn  Annie Oh  Brian Gilger  Rebecca R. Bellone 《Equine veterinary journal》2021,53(2):316-323

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Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages   总被引：3，自引：0，他引：3  

Kim MK  Hossein MS  Oh HJ  Fibrianto HY  Jang G  Kim HJ  Hong SG  Park JE  Kang SK  Lee BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):627-632

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.  相似文献   

Screening of Human CYP1A2 and CYP3A4 Inhibitors from Seaweed In Silico and In Vitro     

Sung-Kun Yim  Kian Kim  SangHo Chun  TaeHawn Oh  WooHuk Jung  KyooJin Jung  Chul-Ho Yun 《Marine drugs》2020,18(12)

Phenolic compounds and carotenoids are potential inhibitors of cytochrome P450s. Sixteen known compounds, phenolic compounds and carotenoids from seaweed were examined for potential inhibitory capacity against CYP1A2 and CYP3A4 in silico and in vitro. Morin, quercetin, and fucoxanthin inhibited the enzyme activity of CYP1A2 and CYP3A4 in a dose-dependent manner. The IC₅₀ values of morin, quercetin, and fucoxanthin were 41.8, 22.5, and 30.3 μM for CYP1A2 and 86.6, 16.1, and 24.4 μM for CYP3A4, respectively. Siphonaxanthin and hesperidin did not show any significant effect on CYP1A2, but they slightly inhibited CYP3A4 activity at high concentrations. In silico modeling of CYP’s binding site revealed that the potential inhibitors bound in the cavity located above the distal surface of the heme prosthetic group through the 2a or 2f channel of CYPs. This study presents an approach for quickly predicting CYP inhibitory activity and shows the potential interactions of compounds and CYPs through in silico modeling.  相似文献   

Symbiosis disruption in the olive fruit fly,Bactrocera oleae (Rossi), as a potential tool for sustainable control     

Martina Sinno  Annie Bzier  Francesco Vinale  David Giron  Stefania Laudonia  Antonio P Garonna  Francesco Pennacchio 《Pest management science》2020,76(9):3199-3207

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Saccharobisindole,Neoasterric Methyl Ester,and 7-Chloro-4(1H)-quinolone: Three New Compounds Isolated from the Marine Bacterium Saccharomonospora sp.     

Sohee Kim  Tu Cam Le  Sang-Ah Han  Prima F. Hillman  Ahreum Hong  Sunghoon Hwang  Young Eun Du  Hiyoung Kim  Dong-Chan Oh  Sun-Shin Cha  Jihye Lee  Sang-Jip Nam  William Fenical 《Marine drugs》2022,20(1)

Analysis of the chemical components from the culture broth of the marine bacterium Saccharomonospora sp. CNQ-490 has yielded three novel compounds: saccharobisindole (1), neoasterric methyl ester (2), and 7-chloro-4(1H)-quinolone (3), in addition to acremonidine E (4), pinselin (5), penicitrinon A (6), and penicitrinon E (7). The chemical structures of the three novel compounds were elucidated by the interpretation of 1D, 2D nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS) data. Compound 2 generated weak inhibition activity against Bacillus subtilis KCTC2441 and Staphylococcus aureus KCTC1927 at concentrations of 32 μg/mL and 64 μg/mL, respectively, whereas compounds 1 and 3 did not have any observable effects. In addition, compound 2 displayed weak anti-quorum sensing (QS) effects against S. aureus KCTC1927 and Micrococcus luteus SCO560.  相似文献   

Compensatory growth and body composition of juvenile black rockfish <Emphasis Type="Italic">Sebastes schlegeli</Emphasis> following feed deprivation   总被引：1，自引：0，他引：1  

Sung-Yong Oh  Choong Hwan Noh  Rae-Seon Kang  Chong-Kwan Kim  Sung Hwoan Cho  Jae-Yoon Jo 《Fisheries Science》2008,74(4):846-852

ABSTRACT:   Compensatory growth, feeding rate, feed efficiency and chemical composition of juvenile black rockfish (mean weight 1.43 g) were investigated for 35 days after a 14-day feed deprivation treatment under four feeding conditions: one group continuously fed (control) and the other three groups fasted for 5 days (F5), 10 days (F10) and 14 days (F14). All fasted fish were re-fed from day 15. Only F5 achieved the same body weight as the control, indicating that complete compensation occurred in F5. The specific growth rate (SGR) of F5 was the highest at day 21 and then decreased thereafter, showing higher values than the control at days 21, 28 and 42. In contrast, although SGRs of F10 and F14 were higher than that of the control during the whole refeeding period except day 21, they did not catch up the control in body mass, indicating that only partial compensation occurred in F10 and F14. The feeding rate (FR) of all groups except F14 changed in a pattern similar to SGR (Spearman's rank correlation, r _s > 0.9), suggesting that SGR varied depending on FR. Similar feeding efficiencies (FEs) were found in the four groups and they did not vary significantly during the whole refeeding period, suggesting that FE was not the factor affecting SGR. At day 14, the ratios of lipid to lean body mass in F10 and F14 were lower than those in the control and F5, and there was no difference between the control and F5. At day 49, however, only F14 showed a lower value than the other three groups, and there was no difference among the three groups. These results indicate that juvenile black rockfish fasted for 5–14 days can exhibit compensatory growth after refeeding, but timing and degree vary depending on the duration of feed deprivation.  相似文献   

The effect of bentonite concentration on the drug delivery efficacy of a pH-sensitive alginate/bentonite hydrogel     

Seung-Taek Oh  Oh-Jin Kwon  Byoung-Chul Chun  Jae-Whan Cho  Jong-Shin Park 《Fibers and Polymers》2009,10(1):21-26

We produced a protein loaded, pH-sensitive alginate-bentonite hydrogel for wound dressings. Alginate is a nontoxic polysaccharide with favorable pH-sensitive properties that make it useful for the intestinal delivery of protein drugs. However, the use of alginate for drug delivery is limited by drug leaching and rapid dissolution of alginate at the higher pH, which may result in lower entrapment efficiency and a burst in the release of entrapped protein drugs. To overcome these problems, we created a novel cross-linked alginate-bentonite hydrogel by combining mineral-rich bentonite with the alginate matrix along with an additive to ensure controlled release. We analyzed the gel in the drug loading process in an aqueous environment by looking at the release profiles of a model protein drug (BSA) from the hydrogel at pH values of 4.5, 5.2 (skin area) and 7.4, 9.2 (wound area). The swelling ratio decreased with bentonite concentration, but did not fall below 6. The rate of drug release was slowest at a pH value of 4.5 and fastest at a pH value of 9.2. The rate of drug release decreased with bentonite concentration. The presence of bentonite prevents the rapid dissolution of alginate at the higher pH, ensuring the controlled release of the entrapped drug.  相似文献   

Utilization of biochar impregnated with anaerobically digested slurry as slow‐release fertilizer          下载免费PDF全文

Taek‐Keun Oh  Yoshiyuki Shinogi  Seung‐Joo Lee  Bongsu Choi 《植物养料与土壤学杂志》2014,177(1):97-103

We examined the possibility of an environment‐friendly slow‐release fertilizer (SRF) made of biochar impregnated by anaerobically digested slurry. The biochar materials were produced from three types of feedstocks (orange peel, residual wood, water‐treatment sludge) at different temperatures of 300°C, 500°C, and 700°C via pyrolysis. The release behaviors of the water‐soluble K⁺, Ca²⁺, and Mg²⁺ were similar for all impregnated biochars and the commercial SRF used. The water‐retention capacity was greatly improved by mixing the biochar‐SRF with the soil. The yield of lettuce was lower for the biochar‐SRF applications of 3.7 to 34.2 t ha^–1 than for the commercial SRF application of 51.4 t ha^–1. This might be due to excessive increase of soil pH for the biochar‐SRF application. Based on these results, the authors concluded that the biochar impregnated with nutrients could become an effective slow‐release K⁺ fertilizer.  相似文献   

Mechanical properties and water purification characteristics of natural jute fiber-reinforced non-cement alkali-activated porous vegetation blocks     

Oh  Ri-On  Cha  Sang-Sun  Park  Seong-Yong  Lee  Hyung-Jun  Park  Sang-Woo  Park  Chan-Gi 《Paddy and Water Environment》2014,12(1):149-156

This study reports the effects of the volume fraction of natural jute fiber and the content of the alkali activator on the physical and mechanical properties, sulfate ion resistance, and water purification characteristics of non-cement porous vegetation blocks. The volume fractions of the natural jute fiber were 0.0, 0.1, and 0.2 %, and the alkali activator was applied by replacing 5, 6, 7, 8, 9, and 10 % by weight of the blast-furnace slag. Void ratio, compressive strength, sulfate resistance, and water purification characteristics were characterized. The results indicate that increasing natural jute fiber and the alkali activator content increased the void ratio and improved compressive strength and sulfate resistance. pH was not affected by natural jute fiber content but increased with alkali activator content. At alkali activator contents of 9–10 %, the observed compressive strength was similar to that of cement blocks, whereas mixes with alkali activator contents of 8–10 % showed similar or greater void ratios than those of cement blocks. The compressive strength of the cement blocks decreased following immersion in sulfate solutions; however, the compressive strength of the mixes with the alkali activator and blast-furnace slag increased following exposure to sulfates. Water purification characteristics were examined by allowing water to filter through the blocks; the non-cement porous vegetation blocks reduced the suspended solids, 5-day biological oxygen demand, chemical oxygen demand, total nitrogen, and total phosphorous in the water by >40 %.  相似文献